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The map label for this gene is wcaL [C]

Identifier: 212225010

GI number: 212225010

Start: 1739589

End: 1740710

Strand: Reverse

Name: wcaL [C]

Synonym: TON_1858

Alternate gene names: 212225010

Gene position: 1740710-1739589 (Counterclockwise)

Preceding gene: 212225011

Following gene: 212225009

Centisome position: 94.21

GC content: 46.52

Gene sequence:

>1122_bases
ATGAAGATAGCAATGCTAATCTCAACGCCGTTTCCACCGGAGGAAGGGATTGGGTATTATACGTATAACCTTTCCAAGAA
GCTAATTGAAAGAGGGCACGAGGTTACGGTAATAACCAGAGGAAGCCCCAGAAACATCGAGCACTTCTATTTCGATGGAA
TCGAAGTGTATAAGCCTCAGTTCTTTCCGGTTTATCCGCTCCACGTTCATATTCACAAGCTGTTTATAGAGAGGTTCTTC
AGAAAATTGGGGAAAGATTTTGATGTTATCCACATACATTCGCCGCTTTCTCCCTTCATCAAACACCCTTTAAACGACAT
CCCAATCGTCTCTACTGTCCATACCTCACTAATTGAGGACATAAAACACTACCAAGTCCACGACTTGAACGCTATGGGAC
AAAAATTAACAACGTATGCAGTGGGATATCCTCTGACAATGGAGCTCCTAGAGAGTTCCGATGTAGTGACAACGGTATCC
TCCGCGGTGGCCAGGGAGATCCAAGAATATTACGGCAGAACCCCGCTAATCTTAGGGAATGGGGTTGATGAAGGCAAGTT
TTACCCGGCAGACAGCAGAGAGGGTGGTTACCTACTCTACGTGGGCAGGCTTGACTACAGAAAAGGTGTGATTGACCTCA
TAAAAGCGGCCCAGATTTTAAAGGAGAACACCACGACCACTAAAATTCTCATTGTAGGCAAAGGACCATTGTACAATGAA
ATCAAGGCAATGATTACCAGGGATAACCTAGACAACGTAGAACTCCTCGGTCACGTTCCCTGGGAACAACTCCTGTGGCT
ATACCGCAACGCGGAGGTCTTCATCTTCCCATCTCATTACGAGGGTCTACCGACGGTGGTCCTTGAGGCTATGGCCTCCG
GCCTTCCAGTTGTTGCCAGTGATATTCCCGCCCATAGGGATGTCATAATAAATGGACACAACGGCCTTCTTTCGAAGAGG
GGCTCTCCAGAGAGTATAGCTGAGAATGTCCTAACACTCCTTGAAAATGAGAAGCTCCAAAGAAAGCTCGGGAGAAACGC
AAGAAAAACCATCGAGAGAAAGTTCACCTGGGGCAAGATAGGCAGGAAATTTGAGAGAATTTACGAGTCAGCCACTAGCT
AA

Upstream 100 bases:

>100_bases
AGGATGCACTAAAAAGGGCCATGTCGATGAAATTCAGGCCACTTAAGAGAAACAGGAAGCTTGTCAATTTCCTGAAAGAA
TACGTGCGAGGGCTCGAAAC

Downstream 100 bases:

>100_bases
GGTGATAGAACTATGAAAGTGGCAATAATCTGCTTCGATTTCAAGGAGTCAAACCTCAGAAAGCAACCCTGGAGATATGT
GTATGAAATTGCAAAGGGGC

Product: phosphatidylinositol glycantransferase-class A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 373; Mature: 373

Protein sequence:

>373_residues
MKIAMLISTPFPPEEGIGYYTYNLSKKLIERGHEVTVITRGSPRNIEHFYFDGIEVYKPQFFPVYPLHVHIHKLFIERFF
RKLGKDFDVIHIHSPLSPFIKHPLNDIPIVSTVHTSLIEDIKHYQVHDLNAMGQKLTTYAVGYPLTMELLESSDVVTTVS
SAVAREIQEYYGRTPLILGNGVDEGKFYPADSREGGYLLYVGRLDYRKGVIDLIKAAQILKENTTTTKILIVGKGPLYNE
IKAMITRDNLDNVELLGHVPWEQLLWLYRNAEVFIFPSHYEGLPTVVLEAMASGLPVVASDIPAHRDVIINGHNGLLSKR
GSPESIAENVLTLLENEKLQRKLGRNARKTIERKFTWGKIGRKFERIYESATS

Sequences:

>Translated_373_residues
MKIAMLISTPFPPEEGIGYYTYNLSKKLIERGHEVTVITRGSPRNIEHFYFDGIEVYKPQFFPVYPLHVHIHKLFIERFF
RKLGKDFDVIHIHSPLSPFIKHPLNDIPIVSTVHTSLIEDIKHYQVHDLNAMGQKLTTYAVGYPLTMELLESSDVVTTVS
SAVAREIQEYYGRTPLILGNGVDEGKFYPADSREGGYLLYVGRLDYRKGVIDLIKAAQILKENTTTTKILIVGKGPLYNE
IKAMITRDNLDNVELLGHVPWEQLLWLYRNAEVFIFPSHYEGLPTVVLEAMASGLPVVASDIPAHRDVIINGHNGLLSKR
GSPESIAENVLTLLENEKLQRKLGRNARKTIERKFTWGKIGRKFERIYESATS
>Mature_373_residues
MKIAMLISTPFPPEEGIGYYTYNLSKKLIERGHEVTVITRGSPRNIEHFYFDGIEVYKPQFFPVYPLHVHIHKLFIERFF
RKLGKDFDVIHIHSPLSPFIKHPLNDIPIVSTVHTSLIEDIKHYQVHDLNAMGQKLTTYAVGYPLTMELLESSDVVTTVS
SAVAREIQEYYGRTPLILGNGVDEGKFYPADSREGGYLLYVGRLDYRKGVIDLIKAAQILKENTTTTKILIVGKGPLYNE
IKAMITRDNLDNVELLGHVPWEQLLWLYRNAEVFIFPSHYEGLPTVVLEAMASGLPVVASDIPAHRDVIINGHNGLLSKR
GSPESIAENVLTLLENEKLQRKLGRNARKTIERKFTWGKIGRKFERIYESATS

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1788357, Length=169, Percent_Identity=28.9940828402367, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001296
- InterPro:   IPR013534 [H]

Pfam domain/function: PF08323 Glyco_transf_5; PF00534 Glycos_transf_1 [H]

EC number: 2.-.-.- [C]

Molecular weight: Translated: 42458; Mature: 42458

Theoretical pI: Translated: 8.55; Mature: 8.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIAMLISTPFPPEEGIGYYTYNLSKKLIERGHEVTVITRGSPRNIEHFYFDGIEVYKPQ
CEEEEEEECCCCCCCCCEEEEEHHHHHHHHCCCEEEEEECCCCCCCCEEEECCEEEECCC
FFPVYPLHVHIHKLFIERFFRKLGKDFDVIHIHSPLSPFIKHPLNDIPIVSTVHTSLIED
CCEEEHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHCCCCCCCHHHHHHHHHHHH
IKHYQVHDLNAMGQKLTTYAVGYPLTMELLESSDVVTTVSSAVAREIQEYYGRTPLILGN
HHHHHHHCHHHHHHHHHHEECCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCEEEEC
GVDEGKFYPADSREGGYLLYVGRLDYRKGVIDLIKAAQILKENTTTTKILIVGKGPLYNE
CCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCHHH
IKAMITRDNLDNVELLGHVPWEQLLWLYRNAEVFIFPSHYEGLPTVVLEAMASGLPVVAS
HHHHHCCCCCCCEEEEECCCHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHCCCCEEEC
DIPAHRDVIINGHNGLLSKRGSPESIAENVLTLLENEKLQRKLGRNARKTIERKFTWGKI
CCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHCHHHHHHHCCHHHHHHHHHCCHHHH
GRKFERIYESATS
HHHHHHHHHHCCC
>Mature Secondary Structure
MKIAMLISTPFPPEEGIGYYTYNLSKKLIERGHEVTVITRGSPRNIEHFYFDGIEVYKPQ
CEEEEEEECCCCCCCCCEEEEEHHHHHHHHCCCEEEEEECCCCCCCCEEEECCEEEECCC
FFPVYPLHVHIHKLFIERFFRKLGKDFDVIHIHSPLSPFIKHPLNDIPIVSTVHTSLIED
CCEEEHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHCCCCCCCHHHHHHHHHHHH
IKHYQVHDLNAMGQKLTTYAVGYPLTMELLESSDVVTTVSSAVAREIQEYYGRTPLILGN
HHHHHHHCHHHHHHHHHHEECCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCEEEEC
GVDEGKFYPADSREGGYLLYVGRLDYRKGVIDLIKAAQILKENTTTTKILIVGKGPLYNE
CCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCHHH
IKAMITRDNLDNVELLGHVPWEQLLWLYRNAEVFIFPSHYEGLPTVVLEAMASGLPVVAS
HHHHHCCCCCCCEEEEECCCHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHCCCCEEEC
DIPAHRDVIINGHNGLLSKRGSPESIAENVLTLLENEKLQRKLGRNARKTIERKFTWGKI
CCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHCHHHHHHHCCHHHHHHHHHCCHHHH
GRKFERIYESATS
HHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]