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The map label for this gene is glnA [H]

Identifier: 212224923

GI number: 212224923

Start: 1637501

End: 1638832

Strand: Reverse

Name: glnA [H]

Synonym: TON_1772

Alternate gene names: 212224923

Gene position: 1638832-1637501 (Counterclockwise)

Preceding gene: 212224927

Following gene: 212224914

Centisome position: 88.7

GC content: 51.95

Gene sequence:

>1332_bases
ATGAACGAAATTGTCAAGGCGGAGGGAACAGATGAAGTCTACACCAAATCCCCAAGATTCATTCAGCTGATATTCGTGGA
CATCAATGGGGTTCCAAAGGGGATGGAGATACCGGCGAGCAGGTACGAGGAGGCAATAACAGATGGAATAACCTTTGATG
GTTCCTCAATCCCAGGCTTTCAGGGAATAGAGGACAGCGACCTCATCTTCAAGGCAGACCCAAGCACCTACGCCGAAATT
CCATGGGAGGGCATAGCCAGAGTTTACGGCTACATCTACAAGGATGAAAAGCCCTATCACGCAGACCCGAGGGGAGTTCT
CAGGAAAGTCCTGGAAAGGCTCGAAAAGGAGGGGTTCACGGCCTACATCGGCCCAGAACCAGAGTTCTACTTATTCAAAA
AGAACGGTTCGTGGGAACTCCACATACCAGACAGCGGAGGCTACTTCGACTTGATAACCCTCGACAAGGCAAGGAGTCTG
AGGAGAGAGATAGCCCTTTACATGCCAGTGTTCGGCCTCGTTCCAGAGGTGCTCCACCACGAGGTTGGGAAGGCTCAGCA
CGAGATAGACTTCAGATTTGACGAGGCACTAAAGACGGCAGACAACATCGTGAGCTTTAAGTACATCGTCAAAGCTATAG
CCGAGATGCGCGGACTCTACGCGACTTTCATGCCCAAACCACTCTACGGCATGCCCGGCAACGGAATGCACCTTCACATA
AGCCTCTGGAAGGGCGGAGAGAACGCTTTCAAAGGTGAGGATGAGCTCAGTGAAACGGCCCTGTACTTCCTCGGGGGAAT
ACTCAAGCACGCAAAGGCTCTAGCTGCAATCACGAACCCCACAGTGAACAGCTACAAGCGTCTGGTTCCGGGTTATGAAG
CTCCAGTCTACATAAGCTGGGGCTACAGGAACAGGAGCGCACTGGTAAGGGTTCCGGCCTTCTGGGGTGACGGTGCAAGG
ATTGAATACCGCTGCCCGGACCCGAGCGCAAACCCATACTTCGCGTTTGCAGCGATATTGATGGCGGGACTTGATGGCAT
TAAGCGGAAGATCGAGCCTGATGCATACGTTGAAGAGAACGTCTACAAGATGAGTGAAAGCAGAAGAGAGGAACTTGGCA
TCGATACACTACCCGGAAGCCTGGGTGAAGCCCTCGAAGAGCTCAGGAAGGACAAAGTTGTCAAAGAGGCCCTCGGAAAG
GCCTACGAAAACTTCATATCTTACAAAGAGCTCGAGTGGGAACGCTACGTTGAATACCTCGAATCAAAGGAGCTGCCACT
GGAGACGAAAAAGGTAACGGAGTGGGAACTGGAGAGGTACTTCCACATCTAA

Upstream 100 bases:

>100_bases
CGTCATAATGACAAGAAATCAACAAAAATGCTTTTATTGGATAAATTGTTCATCAAGTTGTCATTAAAACTGAACACTAG
AACAGTAAATGGTGGTAAAA

Downstream 100 bases:

>100_bases
TGCACTTCCTCGAGGACAACTTCTTCAGGTTTTTCTTCCTCCTTCATGTGAATGAGCATCGAGCCGCCTATGATAAGGGC
TGCCCCTGCGAGTTGTTTTA

Product: glnA glutamine synthetase

Products: NA

Alternate protein names: GS; Glutamate--ammonia ligase [H]

Number of amino acids: Translated: 443; Mature: 443

Protein sequence:

>443_residues
MNEIVKAEGTDEVYTKSPRFIQLIFVDINGVPKGMEIPASRYEEAITDGITFDGSSIPGFQGIEDSDLIFKADPSTYAEI
PWEGIARVYGYIYKDEKPYHADPRGVLRKVLERLEKEGFTAYIGPEPEFYLFKKNGSWELHIPDSGGYFDLITLDKARSL
RREIALYMPVFGLVPEVLHHEVGKAQHEIDFRFDEALKTADNIVSFKYIVKAIAEMRGLYATFMPKPLYGMPGNGMHLHI
SLWKGGENAFKGEDELSETALYFLGGILKHAKALAAITNPTVNSYKRLVPGYEAPVYISWGYRNRSALVRVPAFWGDGAR
IEYRCPDPSANPYFAFAAILMAGLDGIKRKIEPDAYVEENVYKMSESRREELGIDTLPGSLGEALEELRKDKVVKEALGK
AYENFISYKELEWERYVEYLESKELPLETKKVTEWELERYFHI

Sequences:

>Translated_443_residues
MNEIVKAEGTDEVYTKSPRFIQLIFVDINGVPKGMEIPASRYEEAITDGITFDGSSIPGFQGIEDSDLIFKADPSTYAEI
PWEGIARVYGYIYKDEKPYHADPRGVLRKVLERLEKEGFTAYIGPEPEFYLFKKNGSWELHIPDSGGYFDLITLDKARSL
RREIALYMPVFGLVPEVLHHEVGKAQHEIDFRFDEALKTADNIVSFKYIVKAIAEMRGLYATFMPKPLYGMPGNGMHLHI
SLWKGGENAFKGEDELSETALYFLGGILKHAKALAAITNPTVNSYKRLVPGYEAPVYISWGYRNRSALVRVPAFWGDGAR
IEYRCPDPSANPYFAFAAILMAGLDGIKRKIEPDAYVEENVYKMSESRREELGIDTLPGSLGEALEELRKDKVVKEALGK
AYENFISYKELEWERYVEYLESKELPLETKKVTEWELERYFHI
>Mature_443_residues
MNEIVKAEGTDEVYTKSPRFIQLIFVDINGVPKGMEIPASRYEEAITDGITFDGSSIPGFQGIEDSDLIFKADPSTYAEI
PWEGIARVYGYIYKDEKPYHADPRGVLRKVLERLEKEGFTAYIGPEPEFYLFKKNGSWELHIPDSGGYFDLITLDKARSL
RREIALYMPVFGLVPEVLHHEVGKAQHEIDFRFDEALKTADNIVSFKYIVKAIAEMRGLYATFMPKPLYGMPGNGMHLHI
SLWKGGENAFKGEDELSETALYFLGGILKHAKALAAITNPTVNSYKRLVPGYEAPVYISWGYRNRSALVRVPAFWGDGAR
IEYRCPDPSANPYFAFAAILMAGLDGIKRKIEPDAYVEENVYKMSESRREELGIDTLPGSLGEALEELRKDKVVKEALGK
AYENFISYKELEWERYVEYLESKELPLETKKVTEWELERYFHI

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=443, Percent_Identity=25.5079006772009, Blast_Score=115, Evalue=9e-26,
Organism=Escherichia coli, GI1790301, Length=440, Percent_Identity=41.3636363636364, Blast_Score=291, Evalue=4e-80,
Organism=Escherichia coli, GI87081870, Length=451, Percent_Identity=25.4988913525499, Blast_Score=137, Evalue=1e-33,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 50518; Mature: 50518

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS00180 GLNA_1 ; PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEIVKAEGTDEVYTKSPRFIQLIFVDINGVPKGMEIPASRYEEAITDGITFDGSSIPGF
CCCCCCCCCCCHHHCCCCCEEEEEEEEECCCCCCCCCCHHHHHHHHHCCCEECCCCCCCC
QGIEDSDLIFKADPSTYAEIPWEGIARVYGYIYKDEKPYHADPRGVLRKVLERLEKEGFT
CCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCE
AYIGPEPEFYLFKKNGSWELHIPDSGGYFDLITLDKARSLRREIALYMPVFGLVPEVLHH
EEECCCCCEEEEEECCCEEEEECCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHH
EVGKAQHEIDFRFDEALKTADNIVSFKYIVKAIAEMRGLYATFMPKPLYGMPGNGMHLHI
HHCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEE
SLWKGGENAFKGEDELSETALYFLGGILKHAKALAAITNPTVNSYKRLVPGYEAPVYISW
EEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCEEEEE
GYRNRSALVRVPAFWGDGARIEYRCPDPSANPYFAFAAILMAGLDGIKRKIEPDAYVEEN
CCCCCCEEEEEEEEECCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
VYKMSESRREELGIDTLPGSLGEALEELRKDKVVKEALGKAYENFISYKELEWERYVEYL
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ESKELPLETKKVTEWELERYFHI
HCCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNEIVKAEGTDEVYTKSPRFIQLIFVDINGVPKGMEIPASRYEEAITDGITFDGSSIPGF
CCCCCCCCCCCHHHCCCCCEEEEEEEEECCCCCCCCCCHHHHHHHHHCCCEECCCCCCCC
QGIEDSDLIFKADPSTYAEIPWEGIARVYGYIYKDEKPYHADPRGVLRKVLERLEKEGFT
CCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCE
AYIGPEPEFYLFKKNGSWELHIPDSGGYFDLITLDKARSLRREIALYMPVFGLVPEVLHH
EEECCCCCEEEEEECCCEEEEECCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHH
EVGKAQHEIDFRFDEALKTADNIVSFKYIVKAIAEMRGLYATFMPKPLYGMPGNGMHLHI
HHCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEE
SLWKGGENAFKGEDELSETALYFLGGILKHAKALAAITNPTVNSYKRLVPGYEAPVYISW
EEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCEEEEE
GYRNRSALVRVPAFWGDGARIEYRCPDPSANPYFAFAAILMAGLDGIKRKIEPDAYVEEN
CCCCCCEEEEEEEEECCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
VYKMSESRREELGIDTLPGSLGEALEELRKDKVVKEALGKAYENFISYKELEWERYVEYL
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ESKELPLETKKVTEWELERYFHI
HCCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12622808 [H]