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The map label for this gene is alaXS [H]

Identifier: 212224582

GI number: 212224582

Start: 1309274

End: 1309729

Strand: Reverse

Name: alaXS [H]

Synonym: TON_1431

Alternate gene names: 212224582

Gene position: 1309729-1309274 (Counterclockwise)

Preceding gene: 212224585

Following gene: 212224580

Centisome position: 70.89

GC content: 49.78

Gene sequence:

>456_bases
ATGGAGCCAGTTGAGGTTAGAGCTCACACTGCCCTCCACGTGGTCAAGGGTGCGGTCGTTAAAGTTCTTGGCGAGGGTGC
TAAGTGGACGGCGAGCGTTTATGTTGGTGGGAACCATGGAAGGTTAACCGTCAAGTTCGACAGGAAACTAACTCCCGAGG
AAGTAGCAGAGATTGAGCGCCTCGCCAACAAGAAGGTAAAAGAAAACGTTCCAATTCACATCTATGAGCTTCCGAGGAAT
GAGGCCGAAAAAAGATTTGGTGAGGATATGTACGACCTCTTTCCGATACTGCCGGAGGTCAAAACGCTCAAGGTTGCCGT
CATCGAGGACTGGAACGTAAATGCTTGCAAGGAGGAACACACGGCAACGACGGGCGAAATAGGGAGGATAAAGATAAGGA
AGGTTCGCTTTAGGAAGAGCAAGGAGCTTTTGGAGATAAGCTTTGATGTGCTCTGA

Upstream 100 bases:

>100_bases
CATTTATGAACTGAAAAACATTCAAATCAGACTGAATAATCCCTGTTTCAGTTTACCCGTAGCTTTAATAAACATATATT
CCAACCTATTAGGGTGCCTT

Downstream 100 bases:

>100_bases
GTCTGTGTACTGCTACTTCCAAGCGTATATAAAGGGCTGTGTATTTGAAATGCACAAACTCAGGGTCTCACGTAGAGCGC
CGCCCCCATACCGAAGTAGC

Product: alanyl-tRNA synthetase

Products: AMP; diphosphate; L-alanyl-tRNAAla [C]

Alternate protein names: AlaX-S; Alanyl-tRNA deacylase AlaX-S; PhoAlaX [H]

Number of amino acids: Translated: 151; Mature: 151

Protein sequence:

>151_residues
MEPVEVRAHTALHVVKGAVVKVLGEGAKWTASVYVGGNHGRLTVKFDRKLTPEEVAEIERLANKKVKENVPIHIYELPRN
EAEKRFGEDMYDLFPILPEVKTLKVAVIEDWNVNACKEEHTATTGEIGRIKIRKVRFRKSKELLEISFDVL

Sequences:

>Translated_151_residues
MEPVEVRAHTALHVVKGAVVKVLGEGAKWTASVYVGGNHGRLTVKFDRKLTPEEVAEIERLANKKVKENVPIHIYELPRN
EAEKRFGEDMYDLFPILPEVKTLKVAVIEDWNVNACKEEHTATTGEIGRIKIRKVRFRKSKELLEISFDVL
>Mature_151_residues
MEPVEVRAHTALHVVKGAVVKVLGEGAKWTASVYVGGNHGRLTVKFDRKLTPEEVAEIERLANKKVKENVPIHIYELPRN
EAEKRFGEDMYDLFPILPEVKTLKVAVIEDWNVNACKEEHTATTGEIGRIKIRKVRFRKSKELLEISFDVL

Specific function: Functions in trans to edit the amino acid moiety from mischarged charged Ser-tRNA(Ala). Has little activity against Gly- tRNA(Ala) [H]

COG id: COG2872

COG function: function code R; Predicted metal-dependent hydrolases related to alanyl-tRNA synthetase HxxxH domain

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Editing domain AlaX-S subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF07973 tRNA_SAD [H]

EC number: 6.1.1.7 [C]

Molecular weight: Translated: 17201; Mature: 17201

Theoretical pI: Translated: 9.04; Mature: 9.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEPVEVRAHTALHVVKGAVVKVLGEGAKWTASVYVGGNHGRLTVKFDRKLTPEEVAEIER
CCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCEEEEEECCCCCHHHHHHHHH
LANKKVKENVPIHIYELPRNEAEKRFGEDMYDLFPILPEVKTLKVAVIEDWNVNACKEEH
HHCCHHHCCCCEEEEECCCCHHHHHHCCHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCC
TATTGEIGRIKIRKVRFRKSKELLEISFDVL
CCCCCCCCEEEEEEEHHHCCHHHEEEEEECC
>Mature Secondary Structure
MEPVEVRAHTALHVVKGAVVKVLGEGAKWTASVYVGGNHGRLTVKFDRKLTPEEVAEIER
CCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCEEEEEECCCCCHHHHHHHHH
LANKKVKENVPIHIYELPRNEAEKRFGEDMYDLFPILPEVKTLKVAVIEDWNVNACKEEH
HHCCHHHCCCCEEEEECCCCHHHHHHCCHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCC
TATTGEIGRIKIRKVRFRKSKELLEISFDVL
CCCCCCCCEEEEEEEHHHCCHHHEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; @ALAN01.txt*L-alanine!; tRNAAla [C]

Specific reaction: ATP + @ALAN01.txt*L-alanine! + tRNAAla = AMP + diphosphate + L-alanyl-tRNAAla [C]

General reaction: Aminoacylation; Esterification [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9679194 [H]