Click here to switch to the map view.

The map label for this gene is endA

Identifier: 212224568

GI number: 212224568

Start: 1293365

End: 1293880

Strand: Reverse

Name: endA

Synonym: TON_1417

Alternate gene names: 212224568

Gene position: 1293880-1293365 (Counterclockwise)

Preceding gene: 212224569

Following gene: 212224567

Centisome position: 70.03

GC content: 53.1

Gene sequence:

>516_bases
TTGAAGGAGCCGATAGAGTTCAAGCTCAGCGGTGACAGGGCCTTCAGCGAGAGGGAGAAGGCAATAAACCAGCTCTACAA
CAGGCGCTATTTCGGCGAGGTCGTCAACGGAAAGCTCTTTCTTTCGCTCATAGAGGCGGCATACCTGATGGAGAGGGGCA
AGATAAAGGTTCTTGACGGTGGTAAGGAGCTTTCCTTTGAGGAGCTCTTTGAACTCGGCCGGAAAAAAGACGACCAGTTC
GACATCAAATACCTCGTTTACAAAGACCTGCGCGACAGGGGCTATATCGTCAAGTCGGCCCTTAAGTTCGGCTCCCACTT
CAGGGTTTACAGGCGCGGAATGGACGAGCACTCCCAGTGGCTGATATGGGTCGTTCCGGAGAACCTCCGCTTCAGCGCCA
ACGACATAACGGCCCGAGTGAGGGTCGCCCACGGCGTCAGGAAGAACATGGTTATGGCGGTCGTTGATGAGGACAACGAT
GTCGTGTATTATAAGATTGAGTGGGTGAAGTTTTGA

Upstream 100 bases:

>100_bases
GCTTCGGCTTTAGAAAGGTTAAAAGGATAATAGGCTACTACGCCGACGGCGAGGATGCCTACTACATGCTTATGCCGGCC
GACGAGTGGGGTGGAAGGAA

Downstream 100 bases:

>100_bases
TGGTACCTCTCATTGCGCTCTTTCTGTGGCCTTTTCTCTGGTTCCTCCTTCGTGTCGCCTATCACCGTATCTTCCTCGGT
TCCTGGCCGGACCGGGGTTC

Product: tRNA-splicing endonuclease subunit alpha

Products: NA

Alternate protein names: tRNA-intron endonuclease

Number of amino acids: Translated: 171; Mature: 171

Protein sequence:

>171_residues
MKEPIEFKLSGDRAFSEREKAINQLYNRRYFGEVVNGKLFLSLIEAAYLMERGKIKVLDGGKELSFEELFELGRKKDDQF
DIKYLVYKDLRDRGYIVKSALKFGSHFRVYRRGMDEHSQWLIWVVPENLRFSANDITARVRVAHGVRKNMVMAVVDEDND
VVYYKIEWVKF

Sequences:

>Translated_171_residues
MKEPIEFKLSGDRAFSEREKAINQLYNRRYFGEVVNGKLFLSLIEAAYLMERGKIKVLDGGKELSFEELFELGRKKDDQF
DIKYLVYKDLRDRGYIVKSALKFGSHFRVYRRGMDEHSQWLIWVVPENLRFSANDITARVRVAHGVRKNMVMAVVDEDND
VVYYKIEWVKF
>Mature_171_residues
MKEPIEFKLSGDRAFSEREKAINQLYNRRYFGEVVNGKLFLSLIEAAYLMERGKIKVLDGGKELSFEELFELGRKKDDQF
DIKYLVYKDLRDRGYIVKSALKFGSHFRVYRRGMDEHSQWLIWVVPENLRFSANDITARVRVAHGVRKNMVMAVVDEDND
VVYYKIEWVKF

Specific function: Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate

COG id: COG1676

COG function: function code J; tRNA splicing endonuclease

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA-intron endonuclease family. Archaeal short subfamily

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ENDA_THEON (B6YXU4)

Other databases:

- EMBL:   CP000855
- RefSeq:   YP_002307804.1
- GeneID:   7018451
- GenomeReviews:   CP000855_GR
- KEGG:   ton:TON_1417
- HOGENOM:   HBG297289
- OMA:   DFRLYER
- ProtClustDB:   PRK09297
- HAMAP:   MF_01833
- InterPro:   IPR011856
- InterPro:   IPR006676
- InterPro:   IPR006677
- InterPro:   IPR006678
- InterPro:   IPR016442
- Gene3D:   G3DSA:3.40.1350.10
- PIRSF:   PIRSF005285
- TIGRFAMs:   TIGR00324

Pfam domain/function: PF01974 tRNA_int_endo; PF02778 tRNA_int_endo_N; SSF53032 tRNA_int_endo_C; SSF55267 tRNA_int_endo_N

EC number: =3.1.27.9

Molecular weight: Translated: 20287; Mature: 20287

Theoretical pI: Translated: 9.59; Mature: 9.59

Prosite motif: NA

Important sites: ACT_SITE 110-110 ACT_SITE 117-117 ACT_SITE 148-148

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEPIEFKLSGDRAFSEREKAINQLYNRRYFGEVVNGKLFLSLIEAAYLMERGKIKVLDG
CCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCEEEEEC
GKELSFEELFELGRKKDDQFDIKYLVYKDLRDRGYIVKSALKFGSHFRVYRRGMDEHSQW
CCCCCHHHHHHHCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHCHHHHHHHCCCCCCCCE
LIWVVPENLRFSANDITARVRVAHGVRKNMVMAVVDEDNDVVYYKIEWVKF
EEEEECCCCEECCCHHEEEEHHHHCCCCCEEEEEEECCCCEEEEEEEEEEC
>Mature Secondary Structure
MKEPIEFKLSGDRAFSEREKAINQLYNRRYFGEVVNGKLFLSLIEAAYLMERGKIKVLDG
CCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCEEEEEC
GKELSFEELFELGRKKDDQFDIKYLVYKDLRDRGYIVKSALKFGSHFRVYRRGMDEHSQW
CCCCCHHHHHHHCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHCHHHHHHHCCCCCCCCE
LIWVVPENLRFSANDITARVRVAHGVRKNMVMAVVDEDNDVVYYKIEWVKF
EEEEECCCCEECCCHHEEEEHHHHCCCCCEEEEEEECCCCEEEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA