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The map label for this gene is rfcL [H]

Identifier: 212224564

GI number: 212224564

Start: 1289130

End: 1290632

Strand: Reverse

Name: rfcL [H]

Synonym: TON_1413

Alternate gene names: 212224564

Gene position: 1290632-1289130 (Counterclockwise)

Preceding gene: 212224565

Following gene: 212224560

Centisome position: 69.85

GC content: 55.16

Gene sequence:

>1503_bases
ATGGCCATGCCCTCCTCCGATGTTCCCTGGGTTGAGAAGTACCGCCCGAGAAGGCTAAGCGAGATAGTGAACCAGAACAA
GGCTTTGGAGCAGGTAAGGGCATGGATAGAGGCATGGCTGCACGGTAATCCGCCGAAGAAGAAGGCTCTCCTCCTAGCTG
GATCTCCGGGGACGGGCAAGACCACCACCATCTATGCCCTGGCCAGGGAGTACGGCTTCGAGGTCATCGAGCTCAACGCG
AGCGACGAGAGGACCTACGAGAAGATAGAGCGCTATGTCCAGGCAGCCTACACGATGGACATCCTTGGAAAGAGGAGAAA
GCTGATATTCCTCGACGAGTCAGACAACATAGAACCAAGCGGAGCGAGAGAGATAGCGAAGCTCATCGACAAAGCCAGGA
ACCCGATAATCATGTCAGCCAACCACTACTGGGAGGTTCCGAGGGAGATAAGGAACAAGGCCCAGATAGTTGAATACAAG
CGCCTGAGCCAGAGGGACATAATGAAGGCCCTCATAAGGATCCTAAAGGCAGAGGGTGTAACCGTTCCGAAGGAAATCCT
CCAGGAGATAGCCAAGCGCGCCAACGGCGACTTAAGGGCAGCAATAAACGACCTTCAGACTGTTGTCTCGGGTGGAATCG
AGGACGCCAGAGAGGTTCTCGCGTACCGTGACGTGGAGAAGAGCGTCTTCCAGGCATTGGCTCAGCTGTTCGCAACGGAC
AACGCCAAACGCGCAAAGCTGGCCGTTCTCGGCGTTGACATGTTCCCCAATGAACTCCTCCAGTGGATAGACGAGAACCT
GCCTTACGTCTATTACAAGCCGGAGGACATAGCGAGGGCCTACGAAGCGCTTAGCAGAGCTGATATATACCTCGGCAGGG
CCCAGAGGACGGGTAACTACGGACTCTGGAAGTACGCCACGGACATGATGACCGCCGGCGTTGCAGTGGCTGGCGTCAAG
AAGAAGGGCTTCGTGAGGATTTACCCGCCGAAGACGATAAAGCTCCTCACCGAGAGCAAGGGGGAGCGCTCGCTCAGGGA
TTCGGTGGTCAAGAAGATAATGAGCGAGATGCACATGGCGAAGCTCGAGGCCCTTGAAACCCTCAACATCCTCAAGGCTA
TATTCGAGCACAATCCCGACATGGCGGCGCACTTCGTCGTCTTCCTCGACCTGAGTGAGAAGGAGGTCGAGTTCATAGTT
GGCGACAAGGAGAAGGCGAAGACGATATGGGGCAAGAGCCTCAACATTCACAAGCGCCTCAAGGAGGAGCGGAGCGAGCT
CAAGGAGCACGTTAAGGCAGCAGTCGAGACAGCTGAGGTGAAAGAATCCGAAGAAAAAGTCGAGGAAGCAGAAGAGCTCG
AAGAAGAGGGGATAAGCGAAGAGGAGCTCGAAAAGGCAGAGGAAGAGATGGAAGCCGTCGAGAAGGAGAAGAAGGCCGAG
AAGATCGACAAGCCCAAGAAGAAGGGCAAGCAGGCTACGCTCTTTGACTTCCTGAAGAAGTGA

Upstream 100 bases:

>100_bases
CGGACAAGATAGGCGAGTACAACTTCAGGCTCGTTGAGGGAGCGAACGAGATGATTCAGCTCGAGGCTCTGCTCGCTCAG
TTCACCCTAATGGGTAAGTG

Downstream 100 bases:

>100_bases
GGCTTTATTTCAAAATTTTCACGTCCGCACACACTTTGAACACGTGCGGCTTGAAGTCGCTCACCTTTTTTACTTTAACC
TCGCAATCTTTTCCCTGCCT

Product: replication factor C large subunit

Products: NA

Alternate protein names: RFC large subunit; Clamp loader large subunit [H]

Number of amino acids: Translated: 500; Mature: 499

Protein sequence:

>500_residues
MAMPSSDVPWVEKYRPRRLSEIVNQNKALEQVRAWIEAWLHGNPPKKKALLLAGSPGTGKTTTIYALAREYGFEVIELNA
SDERTYEKIERYVQAAYTMDILGKRRKLIFLDESDNIEPSGAREIAKLIDKARNPIIMSANHYWEVPREIRNKAQIVEYK
RLSQRDIMKALIRILKAEGVTVPKEILQEIAKRANGDLRAAINDLQTVVSGGIEDAREVLAYRDVEKSVFQALAQLFATD
NAKRAKLAVLGVDMFPNELLQWIDENLPYVYYKPEDIARAYEALSRADIYLGRAQRTGNYGLWKYATDMMTAGVAVAGVK
KKGFVRIYPPKTIKLLTESKGERSLRDSVVKKIMSEMHMAKLEALETLNILKAIFEHNPDMAAHFVVFLDLSEKEVEFIV
GDKEKAKTIWGKSLNIHKRLKEERSELKEHVKAAVETAEVKESEEKVEEAEELEEEGISEEELEKAEEEMEAVEKEKKAE
KIDKPKKKGKQATLFDFLKK

Sequences:

>Translated_500_residues
MAMPSSDVPWVEKYRPRRLSEIVNQNKALEQVRAWIEAWLHGNPPKKKALLLAGSPGTGKTTTIYALAREYGFEVIELNA
SDERTYEKIERYVQAAYTMDILGKRRKLIFLDESDNIEPSGAREIAKLIDKARNPIIMSANHYWEVPREIRNKAQIVEYK
RLSQRDIMKALIRILKAEGVTVPKEILQEIAKRANGDLRAAINDLQTVVSGGIEDAREVLAYRDVEKSVFQALAQLFATD
NAKRAKLAVLGVDMFPNELLQWIDENLPYVYYKPEDIARAYEALSRADIYLGRAQRTGNYGLWKYATDMMTAGVAVAGVK
KKGFVRIYPPKTIKLLTESKGERSLRDSVVKKIMSEMHMAKLEALETLNILKAIFEHNPDMAAHFVVFLDLSEKEVEFIV
GDKEKAKTIWGKSLNIHKRLKEERSELKEHVKAAVETAEVKESEEKVEEAEELEEEGISEEELEKAEEEMEAVEKEKKAE
KIDKPKKKGKQATLFDFLKK
>Mature_499_residues
AMPSSDVPWVEKYRPRRLSEIVNQNKALEQVRAWIEAWLHGNPPKKKALLLAGSPGTGKTTTIYALAREYGFEVIELNAS
DERTYEKIERYVQAAYTMDILGKRRKLIFLDESDNIEPSGAREIAKLIDKARNPIIMSANHYWEVPREIRNKAQIVEYKR
LSQRDIMKALIRILKAEGVTVPKEILQEIAKRANGDLRAAINDLQTVVSGGIEDAREVLAYRDVEKSVFQALAQLFATDN
AKRAKLAVLGVDMFPNELLQWIDENLPYVYYKPEDIARAYEALSRADIYLGRAQRTGNYGLWKYATDMMTAGVAVAGVKK
KGFVRIYPPKTIKLLTESKGERSLRDSVVKKIMSEMHMAKLEALETLNILKAIFEHNPDMAAHFVVFLDLSEKEVEFIVG
DKEKAKTIWGKSLNIHKRLKEERSELKEHVKAAVETAEVKESEEKVEEAEELEEEGISEEELEKAEEEMEAVEKEKKAEK
IDKPKKKGKQATLFDFLKK

Specific function: Part of the RFC clamp loader complex which loads the PCNA sliding clamp onto DNA (Probable) [H]

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the activator 1 small subunits family. RfcL subfamily [H]

Homologues:

Organism=Homo sapiens, GI31563534, Length=230, Percent_Identity=29.5652173913043, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI4506491, Length=232, Percent_Identity=31.8965517241379, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI31881687, Length=232, Percent_Identity=31.8965517241379, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI6677723, Length=211, Percent_Identity=28.9099526066351, Blast_Score=96, Evalue=6e-20,
Organism=Homo sapiens, GI194306571, Length=207, Percent_Identity=28.5024154589372, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI194306567, Length=207, Percent_Identity=28.5024154589372, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI32528306, Length=328, Percent_Identity=25.609756097561, Blast_Score=80, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17541988, Length=214, Percent_Identity=31.3084112149533, Blast_Score=101, Evalue=7e-22,
Organism=Caenorhabditis elegans, GI71986063, Length=252, Percent_Identity=29.7619047619048, Blast_Score=88, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI17563226, Length=250, Percent_Identity=26.4, Blast_Score=86, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17541368, Length=237, Percent_Identity=26.1603375527426, Blast_Score=78, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17554730, Length=285, Percent_Identity=25.9649122807018, Blast_Score=77, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6324478, Length=230, Percent_Identity=30.8695652173913, Blast_Score=117, Evalue=4e-27,
Organism=Saccharomyces cerevisiae, GI6324039, Length=210, Percent_Identity=32.3809523809524, Blast_Score=102, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6323724, Length=353, Percent_Identity=25.4957507082153, Blast_Score=89, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6322528, Length=223, Percent_Identity=27.8026905829596, Blast_Score=80, Evalue=8e-16,
Organism=Saccharomyces cerevisiae, GI6324791, Length=225, Percent_Identity=24, Blast_Score=71, Evalue=3e-13,
Organism=Drosophila melanogaster, GI17647857, Length=217, Percent_Identity=29.4930875576037, Blast_Score=102, Evalue=6e-22,
Organism=Drosophila melanogaster, GI18859927, Length=215, Percent_Identity=32.093023255814, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI19921076, Length=215, Percent_Identity=29.3023255813954, Blast_Score=96, Evalue=5e-20,
Organism=Drosophila melanogaster, GI17737765, Length=279, Percent_Identity=26.5232974910394, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI62472391, Length=301, Percent_Identity=24.5847176079734, Blast_Score=76, Evalue=5e-14,
Organism=Drosophila melanogaster, GI28574716, Length=183, Percent_Identity=27.3224043715847, Blast_Score=68, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003959 [H]

Pfam domain/function: PF00004 AAA [H]

EC number: NA

Molecular weight: Translated: 57111; Mature: 56980

Theoretical pI: Translated: 7.26; Mature: 7.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAMPSSDVPWVEKYRPRRLSEIVNQNKALEQVRAWIEAWLHGNPPKKKALLLAGSPGTGK
CCCCCCCCCHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCC
TTTIYALAREYGFEVIELNASDERTYEKIERYVQAAYTMDILGKRRKLIFLDESDNIEPS
HHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCC
GAREIAKLIDKARNPIIMSANHYWEVPREIRNKAQIVEYKRLSQRDIMKALIRILKAEGV
CHHHHHHHHHHCCCCEEEECCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
TVPKEILQEIAKRANGDLRAAINDLQTVVSGGIEDAREVLAYRDVEKSVFQALAQLFATD
CCHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
NAKRAKLAVLGVDMFPNELLQWIDENLPYVYYKPEDIARAYEALSRADIYLGRAQRTGNY
CCCCCEEEEEECCCCHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHEECCCCCCCC
GLWKYATDMMTAGVAVAGVKKKGFVRIYPPKTIKLLTESKGERSLRDSVVKKIMSEMHMA
CHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCEEHHHCCCCHHHHHHHHHHHHHHHHHHH
KLEALETLNILKAIFEHNPDMAAHFVVFLDLSEKEVEFIVGDKEKAKTIWGKSLNIHKRL
HHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCEEEEECCHHHHHHHHCCCCHHHHHH
KEERSELKEHVKAAVETAEVKESEEKVEEAEELEEEGISEEELEKAEEEMEAVEKEKKAE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
KIDKPKKKGKQATLFDFLKK
HHCCHHHCCCCHHHHHHHCC
>Mature Secondary Structure 
AMPSSDVPWVEKYRPRRLSEIVNQNKALEQVRAWIEAWLHGNPPKKKALLLAGSPGTGK
CCCCCCCCHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCC
TTTIYALAREYGFEVIELNASDERTYEKIERYVQAAYTMDILGKRRKLIFLDESDNIEPS
HHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCC
GAREIAKLIDKARNPIIMSANHYWEVPREIRNKAQIVEYKRLSQRDIMKALIRILKAEGV
CHHHHHHHHHHCCCCEEEECCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
TVPKEILQEIAKRANGDLRAAINDLQTVVSGGIEDAREVLAYRDVEKSVFQALAQLFATD
CCHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
NAKRAKLAVLGVDMFPNELLQWIDENLPYVYYKPEDIARAYEALSRADIYLGRAQRTGNY
CCCCCEEEEEECCCCHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHEECCCCCCCC
GLWKYATDMMTAGVAVAGVKKKGFVRIYPPKTIKLLTESKGERSLRDSVVKKIMSEMHMA
CHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCEEHHHCCCCHHHHHHHHHHHHHHHHHHH
KLEALETLNILKAIFEHNPDMAAHFVVFLDLSEKEVEFIVGDKEKAKTIWGKSLNIHKRL
HHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCEEEEECCHHHHHHHHCCCCHHHHHH
KEERSELKEHVKAAVETAEVKESEEKVEEAEELEEEGISEEELEKAEEEMEAVEKEKKAE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
KIDKPKKKGKQATLFDFLKK
HHCCHHHCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA