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The map label for this gene is nuoN [H]

Identifier: 212224477

GI number: 212224477

Start: 1203674

End: 1205176

Strand: Reverse

Name: nuoN [H]

Synonym: TON_1326

Alternate gene names: 212224477

Gene position: 1205176-1203674 (Counterclockwise)

Preceding gene: 212224478

Following gene: 212224475

Centisome position: 65.23

GC content: 53.63

Gene sequence:

>1503_bases
GTGATTTCGCTTGTGGTGGCGTTTCCGCTCCTCTTTGCCTTCTTCATCGTCCTGTTGGACACGCTGAGGGTGAAAAGGCA
TATAATACAGGTTTCCTTCCTGCTCGGTGCCATCCTCCCTGCCACGGTTCTCTTCATGGGCATCCCGAAGGGAGAGATAG
TTGGCGGCTGGAACCGGATAAGCGGCATAGAGGTGGGAATAAGCGAACTGAACCTGCCGTTCATCGTAGGGGAGCTCATA
CTCTTTGCCTTTGTGGCACTTTACTCAATATCCTACTTCGACTTCAGGGACAAGAAAACACCCAAGGCTTTAGCTTTGCT
ACTGCTTCTCCACGCCGGCCTTCTGGGAGCGTTCATAGCGGGAGACCTCTTTAACTTCTACATCTACATGGAGATTGCAT
CTGTCTCGGCCTTCGCGCTCATAACCTTCTCGGGCGAGCCGAACTCCAGGAGAGCTGCCTTCAAGTACCTCATGCTCTCG
CTTCTGGCTTCATACTTCTTCGTCTTCGCTATAGGGCTGATATACATGGAGACGGGCTATCTAAACCTGATCCTTATATC
CGAGAACGCTGTCCCCTCAAAAGAGTTGAACGCGGCCGTTGGGATAGCCTTCGCCTCGCTGCTCCTGAAGGCAGGCATCT
TTCCGCTCCACTCATGGCTTCCGGATGCCCATTCGAGTGCTCCCACTCCGGTCAGCGCGGTTCTCTCGGGGGCGGTGGTT
AAAGCTCCTGCCTACGGCTTGATTCTCATGTTCTTAGCCCTTCCTGTAGGAGGGACCTTAAGGACCCTCGCCATGGGTAC
GGCAGTCGTGTCCATTTTCTTTGGCATAACTATGGCGCTCTTACAAAGGGACGCGAAGAGGCTGCTCGCTTACCACACAG
TTTCCCAGATGGGCTATGTGCTTCTCGGGATAGCGAGTCTCAACTTCCTCGGGGCGGCATACTATGCGCTCGCTCATTCA
CTCTTCAAGGGTGGCCTTTTCCTGAGCGTTGGTGCTCTAGGAAGAAAAACTCGGGAGCTGGGGAAGTTCGGCTACAGGAA
CGTGCCAATCATGGCGCTTTCCGTGGTCATGCTCAGTCTGGCCATAGGCGGCGTTTCTCCCTTCATAGGCTCCTACGCGA
AGGGTTTGATCTCTTCTGGCCTCGATGGGGCATGGAAGTATGTCCTCTACGCCGGAAGCGTCGGCACGCTGATTTCCTTC
ACAAAGCTCAACTACTACCTGCTAAGGGGCACCTCTGAGGACATTAAGGCTGTCTGGAAGTTTTCTTCCATCTTCCTCGC
CTTCATCACCTTAGGCTCTGGGCTGTATTTAGGAGCCGTCTTTGATCCAAAGGACATCATATATGTAGTCCTGGCCATGG
TTCTGTTCTTCCTCCTGAAGGCAGCGGATATCTTCAAGAAGAGAGCTCACGTCGGCTTCGGGGAAATAGGGGAGGAAGTG
AACGTCCTCACGGCGGTTTTTATACTGGCAATTCTGGCATCGGTCTTACTCCAGGGGGCGTAG

Upstream 100 bases:

>100_bases
ATCCGCTCCCAGCGACGCTTATGCTCACCACCCTTGTAGTTGATGTCGCGATAACTTCCCTGGCCTTGGCGATTATAATG
CGCATGCGGAGGGATGGGCA

Downstream 100 bases:

>100_bases
AACTACTTCCTTAACTTCTTTCAGCGCCTCAAGTTCTCCTCTCAGTTCTTCCAGCGTGCCACTCATCCCGCTCACGTCTA
TGACCGCAACTATCCCAGCC

Product: putative monovalent cation/H+ antiporter subunit D

Products: NA

Alternate protein names: NADH dehydrogenase I subunit N; NDH-1 subunit N [H]

Number of amino acids: Translated: 500; Mature: 500

Protein sequence:

>500_residues
MISLVVAFPLLFAFFIVLLDTLRVKRHIIQVSFLLGAILPATVLFMGIPKGEIVGGWNRISGIEVGISELNLPFIVGELI
LFAFVALYSISYFDFRDKKTPKALALLLLLHAGLLGAFIAGDLFNFYIYMEIASVSAFALITFSGEPNSRRAAFKYLMLS
LLASYFFVFAIGLIYMETGYLNLILISENAVPSKELNAAVGIAFASLLLKAGIFPLHSWLPDAHSSAPTPVSAVLSGAVV
KAPAYGLILMFLALPVGGTLRTLAMGTAVVSIFFGITMALLQRDAKRLLAYHTVSQMGYVLLGIASLNFLGAAYYALAHS
LFKGGLFLSVGALGRKTRELGKFGYRNVPIMALSVVMLSLAIGGVSPFIGSYAKGLISSGLDGAWKYVLYAGSVGTLISF
TKLNYYLLRGTSEDIKAVWKFSSIFLAFITLGSGLYLGAVFDPKDIIYVVLAMVLFFLLKAADIFKKRAHVGFGEIGEEV
NVLTAVFILAILASVLLQGA

Sequences:

>Translated_500_residues
MISLVVAFPLLFAFFIVLLDTLRVKRHIIQVSFLLGAILPATVLFMGIPKGEIVGGWNRISGIEVGISELNLPFIVGELI
LFAFVALYSISYFDFRDKKTPKALALLLLLHAGLLGAFIAGDLFNFYIYMEIASVSAFALITFSGEPNSRRAAFKYLMLS
LLASYFFVFAIGLIYMETGYLNLILISENAVPSKELNAAVGIAFASLLLKAGIFPLHSWLPDAHSSAPTPVSAVLSGAVV
KAPAYGLILMFLALPVGGTLRTLAMGTAVVSIFFGITMALLQRDAKRLLAYHTVSQMGYVLLGIASLNFLGAAYYALAHS
LFKGGLFLSVGALGRKTRELGKFGYRNVPIMALSVVMLSLAIGGVSPFIGSYAKGLISSGLDGAWKYVLYAGSVGTLISF
TKLNYYLLRGTSEDIKAVWKFSSIFLAFITLGSGLYLGAVFDPKDIIYVVLAMVLFFLLKAADIFKKRAHVGFGEIGEEV
NVLTAVFILAILASVLLQGA
>Mature_500_residues
MISLVVAFPLLFAFFIVLLDTLRVKRHIIQVSFLLGAILPATVLFMGIPKGEIVGGWNRISGIEVGISELNLPFIVGELI
LFAFVALYSISYFDFRDKKTPKALALLLLLHAGLLGAFIAGDLFNFYIYMEIASVSAFALITFSGEPNSRRAAFKYLMLS
LLASYFFVFAIGLIYMETGYLNLILISENAVPSKELNAAVGIAFASLLLKAGIFPLHSWLPDAHSSAPTPVSAVLSGAVV
KAPAYGLILMFLALPVGGTLRTLAMGTAVVSIFFGITMALLQRDAKRLLAYHTVSQMGYVLLGIASLNFLGAAYYALAHS
LFKGGLFLSVGALGRKTRELGKFGYRNVPIMALSVVMLSLAIGGVSPFIGSYAKGLISSGLDGAWKYVLYAGSVGTLISF
TKLNYYLLRGTSEDIKAVWKFSSIFLAFITLGSGLYLGAVFDPKDIIYVVLAMVLFFLLKAADIFKKRAHVGFGEIGEEV
NVLTAVFILAILASVLLQGA

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be a menaquinone. Couples the redox reaction to proton translo

COG id: COG0651

COG function: function code CP; Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 2 family [H]

Homologues:

Organism=Homo sapiens, GI251831116, Length=249, Percent_Identity=28.9156626506024, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI251831117, Length=365, Percent_Identity=23.5616438356164, Blast_Score=77, Evalue=4e-14,
Organism=Escherichia coli, GI1788613, Length=292, Percent_Identity=31.1643835616438, Blast_Score=113, Evalue=4e-26,
Organism=Escherichia coli, GI1788827, Length=318, Percent_Identity=29.874213836478, Blast_Score=109, Evalue=4e-25,
Organism=Escherichia coli, GI1788829, Length=325, Percent_Identity=30.1538461538462, Blast_Score=106, Evalue=4e-24,
Organism=Escherichia coli, GI145693160, Length=382, Percent_Identity=30.1047120418848, Blast_Score=102, Evalue=8e-23,
Organism=Escherichia coli, GI1788831, Length=279, Percent_Identity=32.258064516129, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1788614, Length=252, Percent_Identity=29.7619047619048, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI2367154, Length=331, Percent_Identity=28.0966767371601, Blast_Score=80, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010096
- InterPro:   IPR001750
- InterPro:   IPR003918 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 53842; Mature: 53842

Theoretical pI: Translated: 9.87; Mature: 9.87

Prosite motif: PS00591 GLYCOSYL_HYDROL_F10

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISLVVAFPLLFAFFIVLLDTLRVKRHIIQVSFLLGAILPATVLFMGIPKGEIVGGWNRI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCHHH
SGIEVGISELNLPFIVGELILFAFVALYSISYFDFRDKKTPKALALLLLLHAGLLGAFIA
CCCEECHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
GDLFNFYIYMEIASVSAFALITFSGEPNSRRAAFKYLMLSLLASYFFVFAIGLIYMETGY
HHHHHHHHHEEECCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
LNLILISENAVPSKELNAAVGIAFASLLLKAGIFPLHSWLPDAHSSAPTPVSAVLSGAVV
EEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCHHHHHCCHHH
KAPAYGLILMFLALPVGGTLRTLAMGTAVVSIFFGITMALLQRDAKRLLAYHTVSQMGYV
CCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLGIASLNFLGAAYYALAHSLFKGGLFLSVGALGRKTRELGKFGYRNVPIMALSVVMLSL
HHHHHHHHHHHHHHHHHHHHHHHCCHHEEHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
AIGGVSPFIGSYAKGLISSGLDGAWKYVLYAGSVGTLISFTKLNYYLLRGTSEDIKAVWK
HHCCHHHHHHHHHHHHHHCCCCHHEEHHEEECCHHHHHHHHHHEEEEEECCCHHHHHHHH
FSSIFLAFITLGSGLYLGAVFDPKDIIYVVLAMVLFFLLKAADIFKKRAHVGFGEIGEEV
HHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
NVLTAVFILAILASVLLQGA
HHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MISLVVAFPLLFAFFIVLLDTLRVKRHIIQVSFLLGAILPATVLFMGIPKGEIVGGWNRI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCHHH
SGIEVGISELNLPFIVGELILFAFVALYSISYFDFRDKKTPKALALLLLLHAGLLGAFIA
CCCEECHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
GDLFNFYIYMEIASVSAFALITFSGEPNSRRAAFKYLMLSLLASYFFVFAIGLIYMETGY
HHHHHHHHHEEECCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
LNLILISENAVPSKELNAAVGIAFASLLLKAGIFPLHSWLPDAHSSAPTPVSAVLSGAVV
EEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCHHHHHCCHHH
KAPAYGLILMFLALPVGGTLRTLAMGTAVVSIFFGITMALLQRDAKRLLAYHTVSQMGYV
CCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLGIASLNFLGAAYYALAHSLFKGGLFLSVGALGRKTRELGKFGYRNVPIMALSVVMLSL
HHHHHHHHHHHHHHHHHHHHHHHCCHHEEHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
AIGGVSPFIGSYAKGLISSGLDGAWKYVLYAGSVGTLISFTKLNYYLLRGTSEDIKAVWK
HHCCHHHHHHHHHHHHHHCCCCHHEEHHEEECCHHHHHHHHHHEEEEEECCCHHHHHHHH
FSSIFLAFITLGSGLYLGAVFDPKDIIYVVLAMVLFFLLKAADIFKKRAHVGFGEIGEEV
HHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
NVLTAVFILAILASVLLQGA
HHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA