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The map label for this gene is ypfI [C]

Identifier: 212224117

GI number: 212224117

Start: 890696

End: 893128

Strand: Reverse

Name: ypfI [C]

Synonym: TON_0968

Alternate gene names: 212224117

Gene position: 893128-890696 (Counterclockwise)

Preceding gene: 212224122

Following gene: 212224115

Centisome position: 48.34

GC content: 55.2

Gene sequence:

>2433_bases
GTGACCGTCAAGGTCCGCTTTGATAAGGAAGTGAGAGACTACGCCAAGGGCGAGAAGGTTAAGGATTCCGTTCTCAAGCT
CACCGAGACGGCCCTTGCTCAAGCTTTGGAGAAGTTCCACCGGAGAATGATCGTCATCGAAGGGGACACCCTGAGGAAGG
CCGAGCTGGCCGGAATTCTTGCGGGAGCCTCGGCCAAGGTTCTGAGCGACGTTATAGACGAGCTGAAGAAGAAACGTCTC
CGCGATGAAAGCGAGGATAAAATTGATGTCCTCTACGCCACCGATGCCCTCGGAGAGGAAACCTTCGGAAGGAAGCGCTA
CGAGTCTTTTAGAAAGCACTTCGACGTTTTGGCTGGTTCTAATGTTAACGTTAAAGCCGTAACCTTCAAGCACACCCGGG
ACATACTCGGAAGAACCTATGACCTCCTCGTTCTTGACCTCAGCTACGACTACTCCCCCAACGACCTCGGAAGGATAATC
GAGACGGTTAGGGGAGGCGGCCTAATATTCGTGCTGGCCCACCCATTCGAGAAGTGGAAGAACATGTGGACGGGCTTCCA
CAAGAGCCTTGTTACTCCCCCATACACCATCGACGACGTCAAGAAGCGCTTCAACAGGCGTCTAATCAGAAAGTTCACGG
AGCACGATGGCATCTACATAATTACCGAGAACGGGAAGGTCCGGAAGAAGCCCAAACGGAACAAGAGCCAGGCCAAGATA
AAGGGCAGAAAGGGCGTCGATATTCCCGAAGAAACTATCTTCCCGCGCGAGCTCTACGAGATGGCCTTAACTGAAGGTCA
AGTTGAAGTCCTGAAGGCCTTCGAAGAGCTCGTTGAGAACGAGGGTATGGTCGTTCTCACCGCTGACAGGGGCAGGGGTA
AGAGTGTCTCCGTTGGAATAGCGGCGATAGGCCTTGCCCTGGCACTTAAGAAGCGCACAAGGATAGTAGTCACCGCCCCC
GAACCAGAGAACGTGCAGAGCCTCTTCCGCTTCGCCAAAAGGGCCCTTGAGAGGCTCGGCTTCAAGCCCCACGTCGTTGA
GGAGAAGGGTCTGATAAAGGAGCTCTACGCGAGGAAGATAGGTCTGCGCTATTATCCGCCGACCGAGGGCTACAGAAAAA
GCGCCGACCTCTACATCCTCGACGAGGCCGCCGGAATCCACGTGCCCATACTCCACAAGTATCTGAACAAGCCGCGCGTA
GTTTATTCCTCCACGATTCACGGTTATGAAGGTGCAGGTAGGGGCTTCTCGGTCAAGTTCCTCAAGAAAGCCCGCGAAAA
GAGGGAATTCAAGGAGCTCCACATGGACGAGCCGATACGCTACGCCGAGGGTGATCCCATAGAGAAGTGGCTCTTCGACG
TTCTGCTCCTTGATGCCGAGCCGGTCGAGCTTACCGACGAGGATTACGAGCTGATAAAGAAGAAAGAGGTTTACTTCGAG
GAGCCCGACCTGGACGACTGGTTCGAGAACGACAGGGAAGACCTCAGGAACTTCGTTGGAATCTACATCCTCGCCCACTA
CCGCAACAGGCCGAGCGATGTAGCTTTACTTGCCGATGCCCCACACCACGAGGCCCGCGTTCTCCGCCTGAAGAACGGAA
AGATAGTCACGGCGATACAGATAGCCAAGGAGGGCAACATTCCGAAGAAGGTCATAGATAAGATGGCAAAGGGCTACAAG
CCCCGTGGGAACATAATTCCGGACATGATGGTCAAGCACCACTATTTGAAGGAGTTCGCCAAGCTGAAAGGCTACAGGAT
AGTTAGAATCGCCACCCATCCAGATGCGATGGATATGGGGCTTGGAAGCAAGGCTCTTGAGCTGCTCGAAGAGGAGGCGA
GAAAGAAGGGCCTTGACTGGATAGGCTCGGGCTTTGGAGCGAGCGAGGAGCTCGTCCGCTTCTGGGTTAGGAACGGCTTT
GCGGTGGTCCACCTCAGCCCTGCCAGAAACCCCGTCAGCGGTGAATTCACGGCGATAGTCCTTAAGCCCCTCAGTGAGAA
GGCCAAGAAGCTCATAAAGAAGGCCAACGACGAGTTCCGCATAAGGCTCACCGAGTGGCTGGGCGACACCCACCGCGACC
TCGAACCGGAGATAGCCCGCTGGCTCTTTGAGAGTCCCTTTGGAGAGGCCGTTGACTACCCCATACACCTGACCGAGGTC
CAGAGGAAGCGCCTCGACGCCTTCACGGGTAAGGTGCTCACCTACGATACCGTCGTCGATGCAGTTAAGCCCATCGTCAA
ACTCTACTTCCTCGACGGCTGGATGAAGCCCTACCTCGACGAGCGCCAGATAAAGCTCCTCATCTACCGCGTCCTGCAGG
CCCACAGCTGGGAAGAGACTGCCAAGCTCATTGACAGAACCGAGACCTTCACCATGATAGAGGTGCGCGACATAATCAGG
GGGCTCTGGTACTACTACAAGCGGCTCCTGTGA

Upstream 100 bases:

>100_bases
TTCGTATAAAAGCTTAGCGTTTCGATGATTATCGAGAGACTCTCTGAACCTCCTAACCTTTTTAAGCCCCCTACCCTCTC
TTCTCTGGGAGGTGACGGCC

Downstream 100 bases:

>100_bases
CCGGGCTCTGGTTCTCAGTCTTTTGTTTGTCCTTGGCTATTCCTTGTTCTCTTTCATTCCGCCCCGCCGTTCTGTCTGTT
CTCCGATATTCTGCCGGCAG

Product: ATPase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 810; Mature: 809

Protein sequence:

>810_residues
MTVKVRFDKEVRDYAKGEKVKDSVLKLTETALAQALEKFHRRMIVIEGDTLRKAELAGILAGASAKVLSDVIDELKKKRL
RDESEDKIDVLYATDALGEETFGRKRYESFRKHFDVLAGSNVNVKAVTFKHTRDILGRTYDLLVLDLSYDYSPNDLGRII
ETVRGGGLIFVLAHPFEKWKNMWTGFHKSLVTPPYTIDDVKKRFNRRLIRKFTEHDGIYIITENGKVRKKPKRNKSQAKI
KGRKGVDIPEETIFPRELYEMALTEGQVEVLKAFEELVENEGMVVLTADRGRGKSVSVGIAAIGLALALKKRTRIVVTAP
EPENVQSLFRFAKRALERLGFKPHVVEEKGLIKELYARKIGLRYYPPTEGYRKSADLYILDEAAGIHVPILHKYLNKPRV
VYSSTIHGYEGAGRGFSVKFLKKAREKREFKELHMDEPIRYAEGDPIEKWLFDVLLLDAEPVELTDEDYELIKKKEVYFE
EPDLDDWFENDREDLRNFVGIYILAHYRNRPSDVALLADAPHHEARVLRLKNGKIVTAIQIAKEGNIPKKVIDKMAKGYK
PRGNIIPDMMVKHHYLKEFAKLKGYRIVRIATHPDAMDMGLGSKALELLEEEARKKGLDWIGSGFGASEELVRFWVRNGF
AVVHLSPARNPVSGEFTAIVLKPLSEKAKKLIKKANDEFRIRLTEWLGDTHRDLEPEIARWLFESPFGEAVDYPIHLTEV
QRKRLDAFTGKVLTYDTVVDAVKPIVKLYFLDGWMKPYLDERQIKLLIYRVLQAHSWEETAKLIDRTETFTMIEVRDIIR
GLWYYYKRLL

Sequences:

>Translated_810_residues
MTVKVRFDKEVRDYAKGEKVKDSVLKLTETALAQALEKFHRRMIVIEGDTLRKAELAGILAGASAKVLSDVIDELKKKRL
RDESEDKIDVLYATDALGEETFGRKRYESFRKHFDVLAGSNVNVKAVTFKHTRDILGRTYDLLVLDLSYDYSPNDLGRII
ETVRGGGLIFVLAHPFEKWKNMWTGFHKSLVTPPYTIDDVKKRFNRRLIRKFTEHDGIYIITENGKVRKKPKRNKSQAKI
KGRKGVDIPEETIFPRELYEMALTEGQVEVLKAFEELVENEGMVVLTADRGRGKSVSVGIAAIGLALALKKRTRIVVTAP
EPENVQSLFRFAKRALERLGFKPHVVEEKGLIKELYARKIGLRYYPPTEGYRKSADLYILDEAAGIHVPILHKYLNKPRV
VYSSTIHGYEGAGRGFSVKFLKKAREKREFKELHMDEPIRYAEGDPIEKWLFDVLLLDAEPVELTDEDYELIKKKEVYFE
EPDLDDWFENDREDLRNFVGIYILAHYRNRPSDVALLADAPHHEARVLRLKNGKIVTAIQIAKEGNIPKKVIDKMAKGYK
PRGNIIPDMMVKHHYLKEFAKLKGYRIVRIATHPDAMDMGLGSKALELLEEEARKKGLDWIGSGFGASEELVRFWVRNGF
AVVHLSPARNPVSGEFTAIVLKPLSEKAKKLIKKANDEFRIRLTEWLGDTHRDLEPEIARWLFESPFGEAVDYPIHLTEV
QRKRLDAFTGKVLTYDTVVDAVKPIVKLYFLDGWMKPYLDERQIKLLIYRVLQAHSWEETAKLIDRTETFTMIEVRDIIR
GLWYYYKRLL
>Mature_809_residues
TVKVRFDKEVRDYAKGEKVKDSVLKLTETALAQALEKFHRRMIVIEGDTLRKAELAGILAGASAKVLSDVIDELKKKRLR
DESEDKIDVLYATDALGEETFGRKRYESFRKHFDVLAGSNVNVKAVTFKHTRDILGRTYDLLVLDLSYDYSPNDLGRIIE
TVRGGGLIFVLAHPFEKWKNMWTGFHKSLVTPPYTIDDVKKRFNRRLIRKFTEHDGIYIITENGKVRKKPKRNKSQAKIK
GRKGVDIPEETIFPRELYEMALTEGQVEVLKAFEELVENEGMVVLTADRGRGKSVSVGIAAIGLALALKKRTRIVVTAPE
PENVQSLFRFAKRALERLGFKPHVVEEKGLIKELYARKIGLRYYPPTEGYRKSADLYILDEAAGIHVPILHKYLNKPRVV
YSSTIHGYEGAGRGFSVKFLKKAREKREFKELHMDEPIRYAEGDPIEKWLFDVLLLDAEPVELTDEDYELIKKKEVYFEE
PDLDDWFENDREDLRNFVGIYILAHYRNRPSDVALLADAPHHEARVLRLKNGKIVTAIQIAKEGNIPKKVIDKMAKGYKP
RGNIIPDMMVKHHYLKEFAKLKGYRIVRIATHPDAMDMGLGSKALELLEEEARKKGLDWIGSGFGASEELVRFWVRNGFA
VVHLSPARNPVSGEFTAIVLKPLSEKAKKLIKKANDEFRIRLTEWLGDTHRDLEPEIARWLFESPFGEAVDYPIHLTEVQ
RKRLDAFTGKVLTYDTVVDAVKPIVKLYFLDGWMKPYLDERQIKLLIYRVLQAHSWEETAKLIDRTETFTMIEVRDIIRG
LWYYYKRLL

Specific function: Catalyzes the formation of N(4)-acetylcytidine (ac(4)C) at the wobble position of tRNA(Met), by using acetyl-CoA as an acetyl donor and ATP (or GTP) [H]

COG id: COG1444

COG function: function code R; Predicted P-loop ATPase fused to an acetyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 N-acetyltransferase domain [H]

Homologues:

Organism=Homo sapiens, GI221316723, Length=814, Percent_Identity=26.044226044226, Blast_Score=230, Evalue=3e-60,
Organism=Homo sapiens, GI221316741, Length=814, Percent_Identity=26.044226044226, Blast_Score=230, Evalue=4e-60,
Organism=Escherichia coli, GI1788818, Length=585, Percent_Identity=25.6410256410256, Blast_Score=180, Evalue=3e-46,
Organism=Caenorhabditis elegans, GI17507621, Length=565, Percent_Identity=29.5575221238938, Blast_Score=219, Evalue=3e-57,
Organism=Saccharomyces cerevisiae, GI6324197, Length=932, Percent_Identity=26.3948497854077, Blast_Score=263, Evalue=1e-70,
Organism=Drosophila melanogaster, GI24640662, Length=825, Percent_Identity=27.3939393939394, Blast_Score=260, Evalue=3e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000182
- InterPro:   IPR016181
- InterPro:   IPR013562
- InterPro:   IPR007807 [H]

Pfam domain/function: PF08351 DUF1726; PF05127 DUF699 [H]

EC number: NA

Molecular weight: Translated: 93474; Mature: 93343

Theoretical pI: Translated: 9.76; Mature: 9.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVKVRFDKEVRDYAKGEKVKDSVLKLTETALAQALEKFHRRMIVIEGDTLRKAELAGIL
CEEEEEECHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCHHHHHHHH
AGASAKVLSDVIDELKKKRLRDESEDKIDVLYATDALGEETFGRKRYESFRKHFDVLAGS
CCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCC
NVNVKAVTFKHTRDILGRTYDLLVLDLSYDYSPNDLGRIIETVRGGGLIFVLAHPFEKWK
CCEEEEEEEHHHHHHHCCCEEEEEEEEECCCCCHHHHHHHHHHCCCCEEEEEECCHHHHH
NMWTGFHKSLVTPPYTIDDVKKRFNRRLIRKFTEHDGIYIITENGKVRKKPKRNKSQAKI
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCHHHC
KGRKGVDIPEETIFPRELYEMALTEGQVEVLKAFEELVENEGMVVLTADRGRGKSVSVGI
CCCCCCCCCHHCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCEEEHHH
AAIGLALALKKRTRIVVTAPEPENVQSLFRFAKRALERLGFKPHVVEEKGLIKELYARKI
HHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCEECCCHHHHHHHHHHC
GLRYYPPTEGYRKSADLYILDEAAGIHVPILHKYLNKPRVVYSSTIHGYEGAGRGFSVKF
CCEECCCCCCCCCCCCEEEEECCCCCCHHHHHHHCCCCCEEEECCCCCCCCCCCCCCHHH
LKKAREKREFKELHMDEPIRYAEGDPIEKWLFDVLLLDAEPVELTDEDYELIKKKEVYFE
HHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCC
EPDLDDWFENDREDLRNFVGIYILAHYRNRPSDVALLADAPHHEARVLRLKNGKIVTAIQ
CCCCHHHHCCCHHHHHHHHHHHHEEECCCCCCCEEEEECCCCCCEEEEEECCCCEEEEEE
IAKEGNIPKKVIDKMAKGYKPRGNIIPDMMVKHHYLKEFAKLKGYRIVRIATHPDAMDMG
EECCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHCC
LGSKALELLEEEARKKGLDWIGSGFGASEELVRFWVRNGFAVVHLSPARNPVSGEFTAIV
CCHHHHHHHHHHHHHCCCHHHCCCCCCHHHHHHHHHHCCEEEEEECCCCCCCCCCEEEEE
LKPLSEKAKKLIKKANDEFRIRLTEWLGDTHRDLEPEIARWLFESPFGEAVDYPIHLTEV
EHHHHHHHHHHHHHCCCCEEEEEHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCEEHHHH
QRKRLDAFTGKVLTYDTVVDAVKPIVKLYFLDGWMKPYLDERQIKLLIYRVLQAHSWEET
HHHHHHHHHCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHH
AKLIDRTETFTMIEVRDIIRGLWYYYKRLL
HHHHHCCHHEEHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure 
TVKVRFDKEVRDYAKGEKVKDSVLKLTETALAQALEKFHRRMIVIEGDTLRKAELAGIL
EEEEEECHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCHHHHHHHH
AGASAKVLSDVIDELKKKRLRDESEDKIDVLYATDALGEETFGRKRYESFRKHFDVLAGS
CCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCC
NVNVKAVTFKHTRDILGRTYDLLVLDLSYDYSPNDLGRIIETVRGGGLIFVLAHPFEKWK
CCEEEEEEEHHHHHHHCCCEEEEEEEEECCCCCHHHHHHHHHHCCCCEEEEEECCHHHHH
NMWTGFHKSLVTPPYTIDDVKKRFNRRLIRKFTEHDGIYIITENGKVRKKPKRNKSQAKI
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCHHHC
KGRKGVDIPEETIFPRELYEMALTEGQVEVLKAFEELVENEGMVVLTADRGRGKSVSVGI
CCCCCCCCCHHCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCEEEHHH
AAIGLALALKKRTRIVVTAPEPENVQSLFRFAKRALERLGFKPHVVEEKGLIKELYARKI
HHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCEECCCHHHHHHHHHHC
GLRYYPPTEGYRKSADLYILDEAAGIHVPILHKYLNKPRVVYSSTIHGYEGAGRGFSVKF
CCEECCCCCCCCCCCCEEEEECCCCCCHHHHHHHCCCCCEEEECCCCCCCCCCCCCCHHH
LKKAREKREFKELHMDEPIRYAEGDPIEKWLFDVLLLDAEPVELTDEDYELIKKKEVYFE
HHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCC
EPDLDDWFENDREDLRNFVGIYILAHYRNRPSDVALLADAPHHEARVLRLKNGKIVTAIQ
CCCCHHHHCCCHHHHHHHHHHHHEEECCCCCCCEEEEECCCCCCEEEEEECCCCEEEEEE
IAKEGNIPKKVIDKMAKGYKPRGNIIPDMMVKHHYLKEFAKLKGYRIVRIATHPDAMDMG
EECCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHCC
LGSKALELLEEEARKKGLDWIGSGFGASEELVRFWVRNGFAVVHLSPARNPVSGEFTAIV
CCHHHHHHHHHHHHHCCCHHHCCCCCCHHHHHHHHHHCCEEEEEECCCCCCCCCCEEEEE
LKPLSEKAKKLIKKANDEFRIRLTEWLGDTHRDLEPEIARWLFESPFGEAVDYPIHLTEV
EHHHHHHHHHHHHHCCCCEEEEEHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCEEHHHH
QRKRLDAFTGKVLTYDTVVDAVKPIVKLYFLDGWMKPYLDERQIKLLIYRVLQAHSWEET
HHHHHHHHHCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHH
AKLIDRTETFTMIEVRDIIRGLWYYYKRLL
HHHHHCCHHEEHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12622808 [H]