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The map label for this gene is pyk [H]

Identifier: 212223934

GI number: 212223934

Start: 732788

End: 734209

Strand: Reverse

Name: pyk [H]

Synonym: TON_0785

Alternate gene names: 212223934

Gene position: 734209-732788 (Counterclockwise)

Preceding gene: 212223936

Following gene: 212223933

Centisome position: 39.74

GC content: 51.05

Gene sequence:

>1422_bases
ATGAAGCTGCCACCACACAAGACGAAGATTATAGCAACCGTCGGACCGGCGTCGCTCAATAGGAAAACCCTCAAGGCTAT
GATAAAAGCAGGAATGAGCGTTGCGCGCATAAACTTTGCTCATGGAGACTCCGAGCAGCACGCCAAGACGGTTGAGCTGA
TAAGAGAAGTCTCAGAGAGGCTTAACAGACCCGTTGCTATCCTCGGAGATCTTCCTGGAGTGAAAATACGAGTTGGTGAG
ATTCAAAATGATTCCGTGACGCTGAGGCGCTGGCAAACGATAACCCTTACCACCCGTGACGTTGTGGGCAATGAGGCAGA
GATACCTGTGCAGTTCAAGGACTTTCCTAGAATGGTATCCAAGGGGGATGTCATCTATCTCAGCGACGGCTTCATAGCAC
TTCGTGTCGAGGAGGTCCGCGATACTGACGTGGTCTGTAAAGTCCTTGTTGGGGGCACACTCTTCTCACACAAGGGTATA
AACGTCCCGAACGCAAGGATAGCAATAGATGCCGTGACGGAGAGGGATCTGAAGTTCCTTGGGTTTGCCATTGAGCACGG
TATCGATGCAGTCGGTATAAGTTTTGTTGGCTCGGCCTACGACGTCCTCAAGGTTAGGCGCTTCGTAGAGGAAAGGAACG
GAAAGATGTTTATAATTGCTAAGATCGAGAGGCCCGATGCGGTGAAGAACTTTGACCAAATATTGAGCGCTGCCGATGGG
ATAATGATAGCTAGGGGCGATTTGGGAGTAGAAATGCCAATTGAGAAGCTTCCGGTGCTTCAGAAAAAGCTCATAAAAAA
GGCCAACATGGCGGGCAAGCCTGCAATAACCGCCACCCAAATGCTTGAGTCGATGACCCAAGAGAAGCTCCCCACACGGG
CAGAGGTTACAGACGTAGCCAACGCCATTCTCGATGGAACGGACGCAGTTATGCTCTCGGAGGAGACTGCTGTGGGCAGG
TATCCCATCGATACGGTGAGGATGATGGCCAAGATAGCAAAAACCACCGAAGCCTACCGCGATTCCCTCTGGTCAGCGCG
CGTTGTGGAATGGAAGGGCCAGATGCCAAAGAAAGGGACGATAAAGGACACCATAACGAGGAGCATCATAGAGGCCCTCA
ACTCGATAGACATCAAATACATCCTAACCCCCACAAGAACTGGTGAAACCGCCCGCTTAATATCCCGTTTCAAGCCGAAG
CAGTGGATTTTAGCTTTCGCCACTGACGAGAAGGTTGCCAATAGCCTCATGTTCTCATACGGAGTCTATCCATTCCTCGT
CGAGGAGACCAGCGAGCGCGAGATACTGGGTCTTATCAAAGGTCTGGGCCTAGTGAACGAAAACGACACGGTTCTGCTGA
CGAAGGGAACGCCGATAGGCAAAACTGTGGGGACAAACACGATACGGATATTTACCGTTTGA

Upstream 100 bases:

>100_bases
TCATGCAATCACCGAGATAATTAGGTTCTCCTAATTTATTAATGTTTCGTGGGATAAGATTATTTAAGGTTAAGTTAAAG
TTCCCCCTTAGGTGAACGAC

Downstream 100 bases:

>100_bases
GACCTTTTTCTTCCCATCGACATATTTTAAAAGACTCCTGCGGTTAACACTACATTCGCACAGGGGTGGAATCTATGGAT
CTCGATCAGATAATTGGAAT

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 473; Mature: 473

Protein sequence:

>473_residues
MKLPPHKTKIIATVGPASLNRKTLKAMIKAGMSVARINFAHGDSEQHAKTVELIREVSERLNRPVAILGDLPGVKIRVGE
IQNDSVTLRRWQTITLTTRDVVGNEAEIPVQFKDFPRMVSKGDVIYLSDGFIALRVEEVRDTDVVCKVLVGGTLFSHKGI
NVPNARIAIDAVTERDLKFLGFAIEHGIDAVGISFVGSAYDVLKVRRFVEERNGKMFIIAKIERPDAVKNFDQILSAADG
IMIARGDLGVEMPIEKLPVLQKKLIKKANMAGKPAITATQMLESMTQEKLPTRAEVTDVANAILDGTDAVMLSEETAVGR
YPIDTVRMMAKIAKTTEAYRDSLWSARVVEWKGQMPKKGTIKDTITRSIIEALNSIDIKYILTPTRTGETARLISRFKPK
QWILAFATDEKVANSLMFSYGVYPFLVEETSEREILGLIKGLGLVNENDTVLLTKGTPIGKTVGTNTIRIFTV

Sequences:

>Translated_473_residues
MKLPPHKTKIIATVGPASLNRKTLKAMIKAGMSVARINFAHGDSEQHAKTVELIREVSERLNRPVAILGDLPGVKIRVGE
IQNDSVTLRRWQTITLTTRDVVGNEAEIPVQFKDFPRMVSKGDVIYLSDGFIALRVEEVRDTDVVCKVLVGGTLFSHKGI
NVPNARIAIDAVTERDLKFLGFAIEHGIDAVGISFVGSAYDVLKVRRFVEERNGKMFIIAKIERPDAVKNFDQILSAADG
IMIARGDLGVEMPIEKLPVLQKKLIKKANMAGKPAITATQMLESMTQEKLPTRAEVTDVANAILDGTDAVMLSEETAVGR
YPIDTVRMMAKIAKTTEAYRDSLWSARVVEWKGQMPKKGTIKDTITRSIIEALNSIDIKYILTPTRTGETARLISRFKPK
QWILAFATDEKVANSLMFSYGVYPFLVEETSEREILGLIKGLGLVNENDTVLLTKGTPIGKTVGTNTIRIFTV
>Mature_473_residues
MKLPPHKTKIIATVGPASLNRKTLKAMIKAGMSVARINFAHGDSEQHAKTVELIREVSERLNRPVAILGDLPGVKIRVGE
IQNDSVTLRRWQTITLTTRDVVGNEAEIPVQFKDFPRMVSKGDVIYLSDGFIALRVEEVRDTDVVCKVLVGGTLFSHKGI
NVPNARIAIDAVTERDLKFLGFAIEHGIDAVGISFVGSAYDVLKVRRFVEERNGKMFIIAKIERPDAVKNFDQILSAADG
IMIARGDLGVEMPIEKLPVLQKKLIKKANMAGKPAITATQMLESMTQEKLPTRAEVTDVANAILDGTDAVMLSEETAVGR
YPIDTVRMMAKIAKTTEAYRDSLWSARVVEWKGQMPKKGTIKDTITRSIIEALNSIDIKYILTPTRTGETARLISRFKPK
QWILAFATDEKVANSLMFSYGVYPFLVEETSEREILGLIKGLGLVNENDTVLLTKGTPIGKTVGTNTIRIFTV

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the PEP- utilizing enzyme family [H]

Homologues:

Organism=Homo sapiens, GI10835121, Length=492, Percent_Identity=38.0081300813008, Blast_Score=309, Evalue=4e-84,
Organism=Homo sapiens, GI32967597, Length=492, Percent_Identity=38.0081300813008, Blast_Score=308, Evalue=6e-84,
Organism=Homo sapiens, GI33286418, Length=498, Percent_Identity=37.7510040160643, Blast_Score=305, Evalue=7e-83,
Organism=Homo sapiens, GI33286422, Length=497, Percent_Identity=37.2233400402414, Blast_Score=304, Evalue=1e-82,
Organism=Homo sapiens, GI33286420, Length=497, Percent_Identity=37.2233400402414, Blast_Score=304, Evalue=1e-82,
Organism=Homo sapiens, GI310128732, Length=292, Percent_Identity=41.4383561643836, Blast_Score=209, Evalue=5e-54,
Organism=Homo sapiens, GI310128730, Length=292, Percent_Identity=41.4383561643836, Blast_Score=209, Evalue=5e-54,
Organism=Homo sapiens, GI310128736, Length=241, Percent_Identity=37.7593360995851, Blast_Score=150, Evalue=3e-36,
Organism=Homo sapiens, GI310128734, Length=241, Percent_Identity=37.7593360995851, Blast_Score=150, Evalue=3e-36,
Organism=Homo sapiens, GI310128738, Length=215, Percent_Identity=36.2790697674419, Blast_Score=130, Evalue=3e-30,
Organism=Escherichia coli, GI1787965, Length=466, Percent_Identity=40.343347639485, Blast_Score=328, Evalue=5e-91,
Organism=Escherichia coli, GI1788160, Length=478, Percent_Identity=36.6108786610879, Blast_Score=272, Evalue=4e-74,
Organism=Caenorhabditis elegans, GI17544584, Length=481, Percent_Identity=38.6694386694387, Blast_Score=324, Evalue=6e-89,
Organism=Caenorhabditis elegans, GI71984413, Length=493, Percent_Identity=38.7423935091278, Blast_Score=289, Evalue=3e-78,
Organism=Caenorhabditis elegans, GI17506829, Length=493, Percent_Identity=38.7423935091278, Blast_Score=289, Evalue=3e-78,
Organism=Caenorhabditis elegans, GI71984406, Length=493, Percent_Identity=38.7423935091278, Blast_Score=288, Evalue=3e-78,
Organism=Caenorhabditis elegans, GI17506831, Length=493, Percent_Identity=38.7423935091278, Blast_Score=288, Evalue=4e-78,
Organism=Saccharomyces cerevisiae, GI6319279, Length=485, Percent_Identity=37.1134020618557, Blast_Score=287, Evalue=3e-78,
Organism=Saccharomyces cerevisiae, GI6324923, Length=484, Percent_Identity=35.3305785123967, Blast_Score=276, Evalue=5e-75,
Organism=Drosophila melanogaster, GI24648964, Length=496, Percent_Identity=36.491935483871, Blast_Score=282, Evalue=3e-76,
Organism=Drosophila melanogaster, GI28571814, Length=496, Percent_Identity=36.491935483871, Blast_Score=282, Evalue=4e-76,
Organism=Drosophila melanogaster, GI24648966, Length=414, Percent_Identity=37.6811594202899, Blast_Score=267, Evalue=1e-71,
Organism=Drosophila melanogaster, GI24581235, Length=429, Percent_Identity=34.965034965035, Blast_Score=248, Evalue=5e-66,
Organism=Drosophila melanogaster, GI24646914, Length=271, Percent_Identity=35.0553505535055, Blast_Score=157, Evalue=1e-38,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 52260; Mature: 52260

Theoretical pI: Translated: 9.99; Mature: 9.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLPPHKTKIIATVGPASLNRKTLKAMIKAGMSVARINFAHGDSEQHAKTVELIREVSER
CCCCCCCEEEEEEECCCCCCHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHHH
LNRPVAILGDLPGVKIRVGEIQNDSVTLRRWQTITLTTRDVVGNEAEIPVQFKDFPRMVS
HCCCEEEEECCCCCEEEEEECCCCCEEEEEEEEEEEEEHHHCCCCCCCCEEECHHHHHHC
KGDVIYLSDGFIALRVEEVRDTDVVCKVLVGGTLFSHKGINVPNARIAIDAVTERDLKFL
CCCEEEEECCEEEEEEECCCCHHHHHHHHHCCEEECCCCCCCCCCEEEEEECCHHHHHHH
GFAIEHGIDAVGISFVGSAYDVLKVRRFVEERNGKMFIIAKIERPDAVKNFDQILSAADG
HHHHHCCCCEEHHHHHCCHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHCCC
IMIARGDLGVEMPIEKLPVLQKKLIKKANMAGKPAITATQMLESMTQEKLPTRAEVTDVA
EEEEECCCCCCCCHHHCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHH
NAILDGTDAVMLSEETAVGRYPIDTVRMMAKIAKTTEAYRDSLWSARVVEWKGQMPKKGT
HHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCC
IKDTITRSIIEALNSIDIKYILTPTRTGETARLISRFKPKQWILAFATDEKVANSLMFSY
HHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHH
GVYPFLVEETSEREILGLIKGLGLVNENDTVLLTKGTPIGKTVGTNTIRIFTV
CCCEEEEECCCCHHHHHHHHHCCEECCCCEEEEECCCCCCCCCCCCEEEEEEC
>Mature Secondary Structure
MKLPPHKTKIIATVGPASLNRKTLKAMIKAGMSVARINFAHGDSEQHAKTVELIREVSER
CCCCCCCEEEEEEECCCCCCHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHHH
LNRPVAILGDLPGVKIRVGEIQNDSVTLRRWQTITLTTRDVVGNEAEIPVQFKDFPRMVS
HCCCEEEEECCCCCEEEEEECCCCCEEEEEEEEEEEEEHHHCCCCCCCCEEECHHHHHHC
KGDVIYLSDGFIALRVEEVRDTDVVCKVLVGGTLFSHKGINVPNARIAIDAVTERDLKFL
CCCEEEEECCEEEEEEECCCCHHHHHHHHHCCEEECCCCCCCCCCEEEEEECCHHHHHHH
GFAIEHGIDAVGISFVGSAYDVLKVRRFVEERNGKMFIIAKIERPDAVKNFDQILSAADG
HHHHHCCCCEEHHHHHCCHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHCCC
IMIARGDLGVEMPIEKLPVLQKKLIKKANMAGKPAITATQMLESMTQEKLPTRAEVTDVA
EEEEECCCCCCCCHHHCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHH
NAILDGTDAVMLSEETAVGRYPIDTVRMMAKIAKTTEAYRDSLWSARVVEWKGQMPKKGT
HHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCC
IKDTITRSIIEALNSIDIKYILTPTRTGETARLISRFKPKQWILAFATDEKVANSLMFSY
HHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHH
GVYPFLVEETSEREILGLIKGLGLVNENDTVLLTKGTPIGKTVGTNTIRIFTV
CCCEEEEECCCCHHHHHHHHHCCEECCCCEEEEECCCCCCCCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8436141; 1447787 [H]