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The map label for this gene is prf1 [H]

Identifier: 212223655

GI number: 212223655

Start: 466735

End: 467982

Strand: Reverse

Name: prf1 [H]

Synonym: TON_0507

Alternate gene names: 212223655

Gene position: 467982-466735 (Counterclockwise)

Preceding gene: 212223656

Following gene: 212223652

Centisome position: 25.33

GC content: 53.12

Gene sequence:

>1248_bases
ATGTCTCACAAATCAGCCGAAATGTATGAGCTCAAGAAAAAAGTGGAGGAGCTGAAGAGCTATCGAGGCCGAGCGACCGA
GCTTGTAAGCCTCTACATCCCAGCGGGCTACGACATAAACAAGGTCATGCAACAGCTTAGGGAGGAGTATGGAACTGCCC
AGAATATTAAATCAAAATCCACCCGAAAGAACGTTCTTGGTGCCCTTGAGAGAGCCATGCAGCACCTCAAGCTCTATCGA
AAAACCCCCGAGAACGGTTTGGCCCTTTTCGTTGGAAATGTGAGTGAGCAGGAGGGAGTGAGCGATATAAAACTTTGGGC
CATAGTTCCACCGGAACCCCTTAAGGTTCGCCTTTACCGCTGCGACCAGACCTTTGTAACGGAGCCTCTTGAGGAAATGC
TCCGCGTTAAGGACGCCTACGGTCTAATAACCGTCGAGAAGAACGAAGCCACGATAGGCCTTCTGAGGGGTAAGAGGATT
GAGGTTATCGATGATCTCACCTCAAACGTCCCAGGAAAAACGAGGGCAGGTGGTCAGTCGGCGAGGCGTTACGAGAGGAT
TCGTGAGCAGGAAACCCACGAGTTCATGAAGAGAATCGCCGAGCACGCCGCCAAGGCCTTCCTACCGCTTCTTGAAAAGG
GTGAGCTCAAGGGAATCATCATAGGCGGCCCAGGGCCGACCAAGGAAGAGTTCGTTGATGGCGACTACCTACACCATGAG
CTCAGAAAGAAGGTAATCGGAGTCGTTGACATCAGCTACCACGGCGAATATGGCCTCAGAGAGCTCGTTGAGAAGGCCAG
CGACATACTCAGCGAGCACGAAGCTGTGAAGGAAAGGAAGCTCATCCAGGACTTCTTCAGGCACCTCGTCAAGGACACTG
GGCTGATAACCTACGGCGAGAGGGAAGTCAGGAAGGCTCTAGAGCTCGGTGCCGTTGACACGCTCCTCATCAGCGAGGGC
TACGACAAGGTCAGGGTTAAGGCGAAGTGCAATGCCTGCGGCTGGGAGGAGCTCAAGACAATGAGCGAGCAGGAGTTCCA
TGTTTACAAGAAGAAGCTTACCCACTGCCCGAAGTGCGGCAGTCAGAACATAAGCTTCGAGAAGTGGGACGTCGCCGAGG
AGCTCATAAAGCTGGCAGAAGAGAGCGGTGCCGAGGTTGAGATAATATCCCTCGACACCGAAGAGGGTCAGCAGTTCTAC
AAGGCCTTTGGCGGTCTTGGAGCCTTCTTGAGGTACAAGATTCAGTAA

Upstream 100 bases:

>100_bases
TAGCTCCCTCCAGATTTCGATTTTAGCCTCTATCTAAATCTTTATAAATTCCCTTTTCGTGCCCTTCTTGGGGCGAGAGA
AGTTTTGGAGGGTGATGCTC

Downstream 100 bases:

>100_bases
AGCACCTCCCGGAGGAGCTTTCTGATCTCTTCTTTTACTTCATCTCCGTCGATGATATACGGGGGTCTAAGGACAGGCTT
TATTGAGAAGCCTCTGAGGC

Product: peptide chain release factor 1

Products: NA

Alternate protein names: Translation termination factor aRF1 [H]

Number of amino acids: Translated: 415; Mature: 414

Protein sequence:

>415_residues
MSHKSAEMYELKKKVEELKSYRGRATELVSLYIPAGYDINKVMQQLREEYGTAQNIKSKSTRKNVLGALERAMQHLKLYR
KTPENGLALFVGNVSEQEGVSDIKLWAIVPPEPLKVRLYRCDQTFVTEPLEEMLRVKDAYGLITVEKNEATIGLLRGKRI
EVIDDLTSNVPGKTRAGGQSARRYERIREQETHEFMKRIAEHAAKAFLPLLEKGELKGIIIGGPGPTKEEFVDGDYLHHE
LRKKVIGVVDISYHGEYGLRELVEKASDILSEHEAVKERKLIQDFFRHLVKDTGLITYGEREVRKALELGAVDTLLISEG
YDKVRVKAKCNACGWEELKTMSEQEFHVYKKKLTHCPKCGSQNISFEKWDVAEELIKLAEESGAEVEIISLDTEEGQQFY
KAFGGLGAFLRYKIQ

Sequences:

>Translated_415_residues
MSHKSAEMYELKKKVEELKSYRGRATELVSLYIPAGYDINKVMQQLREEYGTAQNIKSKSTRKNVLGALERAMQHLKLYR
KTPENGLALFVGNVSEQEGVSDIKLWAIVPPEPLKVRLYRCDQTFVTEPLEEMLRVKDAYGLITVEKNEATIGLLRGKRI
EVIDDLTSNVPGKTRAGGQSARRYERIREQETHEFMKRIAEHAAKAFLPLLEKGELKGIIIGGPGPTKEEFVDGDYLHHE
LRKKVIGVVDISYHGEYGLRELVEKASDILSEHEAVKERKLIQDFFRHLVKDTGLITYGEREVRKALELGAVDTLLISEG
YDKVRVKAKCNACGWEELKTMSEQEFHVYKKKLTHCPKCGSQNISFEKWDVAEELIKLAEESGAEVEIISLDTEEGQQFY
KAFGGLGAFLRYKIQ
>Mature_414_residues
SHKSAEMYELKKKVEELKSYRGRATELVSLYIPAGYDINKVMQQLREEYGTAQNIKSKSTRKNVLGALERAMQHLKLYRK
TPENGLALFVGNVSEQEGVSDIKLWAIVPPEPLKVRLYRCDQTFVTEPLEEMLRVKDAYGLITVEKNEATIGLLRGKRIE
VIDDLTSNVPGKTRAGGQSARRYERIREQETHEFMKRIAEHAAKAFLPLLEKGELKGIIIGGPGPTKEEFVDGDYLHHEL
RKKVIGVVDISYHGEYGLRELVEKASDILSEHEAVKERKLIQDFFRHLVKDTGLITYGEREVRKALELGAVDTLLISEGY
DKVRVKAKCNACGWEELKTMSEQEFHVYKKKLTHCPKCGSQNISFEKWDVAEELIKLAEESGAEVEIISLDTEEGQQFYK
AFGGLGAFLRYKIQ

Specific function: Directs the termination of nascent peptide synthesis (translation) in response to the termination codons UAA, UAG and UGA [H]

COG id: COG1503

COG function: function code J; Peptide chain release factor 1 (eRF1)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the eukaryotic release factor 1 family [H]

Homologues:

Organism=Homo sapiens, GI4759034, Length=415, Percent_Identity=33.2530120481928, Blast_Score=252, Evalue=4e-67,
Organism=Caenorhabditis elegans, GI72000368, Length=413, Percent_Identity=33.6561743341404, Blast_Score=244, Evalue=8e-65,
Organism=Saccharomyces cerevisiae, GI6319619, Length=412, Percent_Identity=33.7378640776699, Blast_Score=240, Evalue=3e-64,
Organism=Drosophila melanogaster, GI28574632, Length=417, Percent_Identity=34.7721822541966, Blast_Score=246, Evalue=2e-65,
Organism=Drosophila melanogaster, GI21356433, Length=417, Percent_Identity=34.7721822541966, Blast_Score=246, Evalue=2e-65,
Organism=Drosophila melanogaster, GI24667392, Length=417, Percent_Identity=34.7721822541966, Blast_Score=246, Evalue=2e-65,
Organism=Drosophila melanogaster, GI24667388, Length=417, Percent_Identity=34.7721822541966, Blast_Score=246, Evalue=2e-65,
Organism=Drosophila melanogaster, GI24667380, Length=417, Percent_Identity=34.7721822541966, Blast_Score=246, Evalue=2e-65,
Organism=Drosophila melanogaster, GI24667384, Length=417, Percent_Identity=34.7721822541966, Blast_Score=246, Evalue=2e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005140
- InterPro:   IPR005141
- InterPro:   IPR005142
- InterPro:   IPR020918
- InterPro:   IPR004403 [H]

Pfam domain/function: PF03463 eRF1_1; PF03464 eRF1_2; PF03465 eRF1_3 [H]

EC number: NA

Molecular weight: Translated: 47282; Mature: 47151

Theoretical pI: Translated: 7.34; Mature: 7.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHKSAEMYELKKKVEELKSYRGRATELVSLYIPAGYDINKVMQQLREEYGTAQNIKSKS
CCCCHHHHHHHHHHHHHHHHHCCCHHEEEEEEECCCCCHHHHHHHHHHHHCCHHHHHHHH
TRKNVLGALERAMQHLKLYRKTPENGLALFVGNVSEQEGVSDIKLWAIVPPEPLKVRLYR
HHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCEEEEEECCCCCCEEEEEE
CDQTFVTEPLEEMLRVKDAYGLITVEKNEATIGLLRGKRIEVIDDLTSNVPGKTRAGGQS
CCHHHHHHHHHHHHHHHHHCCEEEEECCCCEEEEECCCCHHHHHHHHCCCCCCCCCCCHH
ARRYERIREQETHEFMKRIAEHAAKAFLPLLEKGELKGIIIGGPGPTKEEFVDGDYLHHE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHCCHHHHHHH
LRKKVIGVVDISYHGEYGLRELVEKASDILSEHEAVKERKLIQDFFRHLVKDTGLITYGE
HHHHHHEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECH
REVRKALELGAVDTLLISEGYDKVRVKAKCNACGWEELKTMSEQEFHVYKKKLTHCPKCG
HHHHHHHHHCCHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
SQNISFEKWDVAEELIKLAEESGAEVEIISLDTEEGQQFYKAFGGLGAFLRYKIQ
CCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHCHHHHHEEECC
>Mature Secondary Structure 
SHKSAEMYELKKKVEELKSYRGRATELVSLYIPAGYDINKVMQQLREEYGTAQNIKSKS
CCCHHHHHHHHHHHHHHHHHCCCHHEEEEEEECCCCCHHHHHHHHHHHHCCHHHHHHHH
TRKNVLGALERAMQHLKLYRKTPENGLALFVGNVSEQEGVSDIKLWAIVPPEPLKVRLYR
HHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCEEEEEECCCCCCEEEEEE
CDQTFVTEPLEEMLRVKDAYGLITVEKNEATIGLLRGKRIEVIDDLTSNVPGKTRAGGQS
CCHHHHHHHHHHHHHHHHHCCEEEEECCCCEEEEECCCCHHHHHHHHCCCCCCCCCCCHH
ARRYERIREQETHEFMKRIAEHAAKAFLPLLEKGELKGIIIGGPGPTKEEFVDGDYLHHE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHCCHHHHHHH
LRKKVIGVVDISYHGEYGLRELVEKASDILSEHEAVKERKLIQDFFRHLVKDTGLITYGE
HHHHHHEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECH
REVRKALELGAVDTLLISEGYDKVRVKAKCNACGWEELKTMSEQEFHVYKKKLTHCPKCG
HHHHHHHHHCCHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
SQNISFEKWDVAEELIKLAEESGAEVEIISLDTEEGQQFYKAFGGLGAFLRYKIQ
CCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHCHHHHHEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA