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The map label for this gene is pheT [C]

Identifier: 212223592

GI number: 212223592

Start: 418875

End: 419600

Strand: Reverse

Name: pheT [C]

Synonym: TON_0446

Alternate gene names: 212223592

Gene position: 419600-418875 (Counterclockwise)

Preceding gene: 212223593

Following gene: 212223591

Centisome position: 22.71

GC content: 56.61

Gene sequence:

>726_bases
ATGTGGGACACGAGCAAGGACTACCGCTTACTGGTGGCGGAGAAGAGCGTGGAGCTGTTCCTGAAGACGATTGAGGGAGC
AAAGTTCAAGGGCAAGTGGGACAAGAAGAAGGCCATCCAGCTTGCCAAGGAGATGATCCCCGAGATACAGGCCATGCGCT
ACTCCTATATCGAGCCGAAGGAACTCATAGAGACTCCGCAGATGCAGGCTCTGAAGGAGAAGGCCAACGGCATAATCGAG
GCTCTTGGTGGAGACGACTGGCATCACAAGTTCCTCAGCCTGGCCGACAAGAGCGAGCGCGAGAAGGTTGAGGAGGCTGT
GGCAAAAATCAGGTTTTTCCTCAACACTATCCTCAACCTTGACAAGCGCCTAGCATTAGGTAAGATAAACGATCCGGTCA
TAGCGGTGGACATAAGGGTCGGCGAGGTCATGAGCGTCGGGAAGCACCCCAACGCGGACAGGCTTTTGGTTACGAACGTC
AACATCGGCGAGAGGGCAATAACTGTGGTCACCAACGACCTCACCGTTAAGGAGGGTAACCGCGTTGCCGTTGCCCTGCT
CCCGCCGGCGAACTTCCGCGGAATAGTCAGCGAGGGGATGTTCCTCGGCGCCGGTGAAGGCGTCCTCAAGGACGTCAAGG
GAGAGATCGGCGGCCTGCCGAAGGGAATCCCGCTCGAGGCATTCAACGAGACGAGGAACCTTGTAGAGGCGTTTTTGAAG
GGGTGA

Upstream 100 bases:

>100_bases
GCACGCTGGGGAGACAAAGCGAAGGCCCGTTCTATAGCTGAGGGAATAAGGGACGAAAGCGTTAAGAACCGCGTTCTGAA
AGCTCTGGAGGGTGAAGAAG

Downstream 100 bases:

>100_bases
AATATTATCCCCCAGACTAGAATTTTGAGGTATTCTTTTTTCTTCTAAGGTTTCTGTGACATTTCTGGTGTATTCCACCT
CTTTCACATTTTACATTTAT

Product: putative tRNA-binding protein

Products: AMP; diphosphate; L-phenylalanyl-tRNAPhe [C]

Alternate protein names: None

Number of amino acids: Translated: 241; Mature: 241

Protein sequence:

>241_residues
MWDTSKDYRLLVAEKSVELFLKTIEGAKFKGKWDKKKAIQLAKEMIPEIQAMRYSYIEPKELIETPQMQALKEKANGIIE
ALGGDDWHHKFLSLADKSEREKVEEAVAKIRFFLNTILNLDKRLALGKINDPVIAVDIRVGEVMSVGKHPNADRLLVTNV
NIGERAITVVTNDLTVKEGNRVAVALLPPANFRGIVSEGMFLGAGEGVLKDVKGEIGGLPKGIPLEAFNETRNLVEAFLK
G

Sequences:

>Translated_241_residues
MWDTSKDYRLLVAEKSVELFLKTIEGAKFKGKWDKKKAIQLAKEMIPEIQAMRYSYIEPKELIETPQMQALKEKANGIIE
ALGGDDWHHKFLSLADKSEREKVEEAVAKIRFFLNTILNLDKRLALGKINDPVIAVDIRVGEVMSVGKHPNADRLLVTNV
NIGERAITVVTNDLTVKEGNRVAVALLPPANFRGIVSEGMFLGAGEGVLKDVKGEIGGLPKGIPLEAFNETRNLVEAFLK
G
>Mature_241_residues
MWDTSKDYRLLVAEKSVELFLKTIEGAKFKGKWDKKKAIQLAKEMIPEIQAMRYSYIEPKELIETPQMQALKEKANGIIE
ALGGDDWHHKFLSLADKSEREKVEEAVAKIRFFLNTILNLDKRLALGKINDPVIAVDIRVGEVMSVGKHPNADRLLVTNV
NIGERAITVVTNDLTVKEGNRVAVALLPPANFRGIVSEGMFLGAGEGVLKDVKGEIGGLPKGIPLEAFNETRNLVEAFLK
G

Specific function: Unknown

COG id: COG2517

COG function: function code R; Predicted RNA-binding protein containing a C-terminal EMAP domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 6.1.1.20 [C]

Molecular weight: Translated: 26734; Mature: 26734

Theoretical pI: Translated: 8.81; Mature: 8.81

Prosite motif: PS50886 TRBD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWDTSKDYRLLVAEKSVELFLKTIEGAKFKGKWDKKKAIQLAKEMIPEIQAMRYSYIEPK
CCCCCCCCEEEEEHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHH
ELIETPQMQALKEKANGIIEALGGDDWHHKFLSLADKSEREKVEEAVAKIRFFLNTILNL
HHHCCCHHHHHHHHHHHHEECCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHH
DKRLALGKINDPVIAVDIRVGEVMSVGKHPNADRLLVTNVNIGERAITVVTNDLTVKEGN
HHHHHCCCCCCCEEEEEEEHHHHHHCCCCCCCCEEEEEECCCCCEEEEEEECCEEEECCC
RVAVALLPPANFRGIVSEGMFLGAGEGVLKDVKGEIGGLPKGIPLEAFNETRNLVEAFLK
EEEEEEECCCCCCHHHHCCEEEECCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHC
G
C
>Mature Secondary Structure
MWDTSKDYRLLVAEKSVELFLKTIEGAKFKGKWDKKKAIQLAKEMIPEIQAMRYSYIEPK
CCCCCCCCEEEEEHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHH
ELIETPQMQALKEKANGIIEALGGDDWHHKFLSLADKSEREKVEEAVAKIRFFLNTILNL
HHHCCCHHHHHHHHHHHHEECCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHH
DKRLALGKINDPVIAVDIRVGEVMSVGKHPNADRLLVTNVNIGERAITVVTNDLTVKEGN
HHHHHCCCCCCCEEEEEEEHHHHHHCCCCCCCCEEEEEECCCCCEEEEEEECCEEEECCC
RVAVALLPPANFRGIVSEGMFLGAGEGVLKDVKGEIGGLPKGIPLEAFNETRNLVEAFLK
EEEEEEECCCCCCHHHHCCEEEECCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHC
G
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Mg2+; Zn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.125 {2'-deoxyadenosine} 0.009 {ATP}} 0.2 {2-chloroadenosine} 0.11 {2-chloroadenosine} [C]

Substrates: ATP; @PHEN01.txt*phenylalanine!; tRNAPhe [C]

Specific reaction: ATP + @PHEN01.txt*phenylalanine! + tRNAPhe = AMP + diphosphate + L-phenylalanyl-tRNAPhe [C]

General reaction: Aminoacylation; Esterification [C]

Inhibitor: 3'-Deoxyadenosine 5'-triphosphate; 3'-Methyl adenosine 5'-triphosphate; Zn2+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA