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The map label for this gene is speB [C]

Identifier: 212223571

GI number: 212223571

Start: 397738

End: 398601

Strand: Reverse

Name: speB [C]

Synonym: TON_0425

Alternate gene names: 212223571

Gene position: 398601-397738 (Counterclockwise)

Preceding gene: 212223572

Following gene: 212223567

Centisome position: 21.57

GC content: 53.01

Gene sequence:

>864_bases
ATGGACTTCCTCTACACTTACGAAACACTTAAGCTCGAATTCCCTCTCGTTGAGCCTGAAGAAGCAAAATTCGTACTCCT
CGGTGTCCCCTTCGATGGAACCACGAGCTTCAAGCCAGGAACAAGATTCGGGCCCACACTCATAAGGCACGCCACGCTCA
ACCTCGAGAGCTACATCCTTGACTATGACATAGACATAGCCGGGCTTCCAATAGCTGATATAGGAGATATCGCAGTCATA
GCAGGTGATGCAAAAAAAACTGCCGAGCGCGTTAGGGAGACCATCGAGGAGCTGAAAAGAGTGAATCCGGAAGCGATACC
GATAACCCTCGGCGGTGAACATTCAATAACCCTTGGTCCAGTGGAAGCGCTGAATCCGGCAAGCTACGTTGTCTTCGATG
CTCACCTCGACCTTCGCGACCAGTACGAAGACAACCCCTACAGCCACGCCTGCGTCGCCAGAAGAATAAGCGAACTCGGC
GTCAATGAGGCAATGTTTGGCATAAGGAGCGGGACAAAGGAGGAAGTCGAGTACGCCGAAGAGAACGGCATCGCTTGGGT
TCACGCCAGGGACTACGATTTCGATGCATTCGTCGAACTCGTGAAGCCCCTGCCGGAGCCGGTTTACCTATCCATCGATA
TCGATGTTTTCGACCTCTCGCTCGTTCCATCAACCGGAACACCCGAAGCTGGCGGCTTGGCCTTCTGGGACATCATCGAG
GCTATCGAGTGGCTGGCAGAAAACAAGAAAATTGCTGGCTTCGATATCATGGAAGTCGCCGGAACCGAACTGGGGGATGT
AACTGCACTCACAGCGGCAAAGCTGCTCTTTTACTTCATTGGGGCTATGGGGAAGGGGCCATAG

Upstream 100 bases:

>100_bases
GATCATGAAGATAAAGGGCGAGTGATTCTCTTCTCGTCTTTCCTTCCATTTCTGCGCAACCTTTTAAACAGCTTATTTAA
TTCTCTCCGGAGGTGAGGGT

Downstream 100 bases:

>100_bases
GGTTAGGAGGAAAGCCTGTAGCCGGCCGCCCTCATCTCTTTTTCAATGCTCCTTACCTCGAGAAGCCGTTTCCTGAGGAA
GGGAACCCAGAGCGTTTCCA

Product: Agmatinase

Products: Putrescine; Urea [C]

Alternate protein names: NA

Number of amino acids: Translated: 287; Mature: 287

Protein sequence:

>287_residues
MDFLYTYETLKLEFPLVEPEEAKFVLLGVPFDGTTSFKPGTRFGPTLIRHATLNLESYILDYDIDIAGLPIADIGDIAVI
AGDAKKTAERVRETIEELKRVNPEAIPITLGGEHSITLGPVEALNPASYVVFDAHLDLRDQYEDNPYSHACVARRISELG
VNEAMFGIRSGTKEEVEYAEENGIAWVHARDYDFDAFVELVKPLPEPVYLSIDIDVFDLSLVPSTGTPEAGGLAFWDIIE
AIEWLAENKKIAGFDIMEVAGTELGDVTALTAAKLLFYFIGAMGKGP

Sequences:

>Translated_287_residues
MDFLYTYETLKLEFPLVEPEEAKFVLLGVPFDGTTSFKPGTRFGPTLIRHATLNLESYILDYDIDIAGLPIADIGDIAVI
AGDAKKTAERVRETIEELKRVNPEAIPITLGGEHSITLGPVEALNPASYVVFDAHLDLRDQYEDNPYSHACVARRISELG
VNEAMFGIRSGTKEEVEYAEENGIAWVHARDYDFDAFVELVKPLPEPVYLSIDIDVFDLSLVPSTGTPEAGGLAFWDIIE
AIEWLAENKKIAGFDIMEVAGTELGDVTALTAAKLLFYFIGAMGKGP
>Mature_287_residues
MDFLYTYETLKLEFPLVEPEEAKFVLLGVPFDGTTSFKPGTRFGPTLIRHATLNLESYILDYDIDIAGLPIADIGDIAVI
AGDAKKTAERVRETIEELKRVNPEAIPITLGGEHSITLGPVEALNPASYVVFDAHLDLRDQYEDNPYSHACVARRISELG
VNEAMFGIRSGTKEEVEYAEENGIAWVHARDYDFDAFVELVKPLPEPVYLSIDIDVFDLSLVPSTGTPEAGGLAFWDIIE
AIEWLAENKKIAGFDIMEVAGTELGDVTALTAAKLLFYFIGAMGKGP

Specific function: Catalyzes The Formation Of Putrescine From Agmatine. [C]

COG id: COG0010

COG function: function code E; Arginase/agmatinase/formimionoglutamate hydrolase, arginase family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the arginase family [H]

Homologues:

Organism=Homo sapiens, GI37537722, Length=290, Percent_Identity=32.0689655172414, Blast_Score=119, Evalue=4e-27,
Organism=Escherichia coli, GI1789306, Length=254, Percent_Identity=30.3149606299213, Blast_Score=95, Evalue=5e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005925
- InterPro:   IPR006035
- InterPro:   IPR020855 [H]

Pfam domain/function: PF00491 Arginase [H]

EC number: 3.5.3.11 [C]

Molecular weight: Translated: 31562; Mature: 31562

Theoretical pI: Translated: 4.09; Mature: 4.09

Prosite motif: PS00147 ARGINASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFLYTYETLKLEFPLVEPEEAKFVLLGVPFDGTTSFKPGTRFGPTLIRHATLNLESYIL
CCEEEEEEEEEEEECCCCCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHEEECHHHEEE
DYDIDIAGLPIADIGDIAVIAGDAKKTAERVRETIEELKRVNPEAIPITLGGEHSITLGP
EECCEECCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEECC
VEALNPASYVVFDAHLDLRDQYEDNPYSHACVARRISELGVNEAMFGIRSGTKEEVEYAE
HHHCCCCEEEEEEEEECCHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHCCCCHHHHHHHH
ENGIAWVHARDYDFDAFVELVKPLPEPVYLSIDIDVFDLSLVPSTGTPEAGGLAFWDIIE
CCCEEEEEECCCCHHHHHHHHHCCCCCEEEEEEEEEEEEEEECCCCCCCCCCCHHHHHHH
AIEWLAENKKIAGFDIMEVAGTELGDVTALTAAKLLFYFIGAMGKGP
HHHHHHCCCCCCCEEHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MDFLYTYETLKLEFPLVEPEEAKFVLLGVPFDGTTSFKPGTRFGPTLIRHATLNLESYIL
CCEEEEEEEEEEEECCCCCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHEEECHHHEEE
DYDIDIAGLPIADIGDIAVIAGDAKKTAERVRETIEELKRVNPEAIPITLGGEHSITLGP
EECCEECCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEECC
VEALNPASYVVFDAHLDLRDQYEDNPYSHACVARRISELGVNEAMFGIRSGTKEEVEYAE
HHHCCCCEEEEEEEEECCHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHCCCCHHHHHHHH
ENGIAWVHARDYDFDAFVELVKPLPEPVYLSIDIDVFDLSLVPSTGTPEAGGLAFWDIIE
CCCEEEEEECCCCHHHHHHHHHCCCCCEEEEEEEEEEEEEEECCCCCCCCCCCHHHHHHH
AIEWLAENKKIAGFDIMEVAGTELGDVTALTAAKLLFYFIGAMGKGP
HHHHHHCCCCCCCEEHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Agmatine; H2O [C]

Specific reaction: Agmatine + H2O --> Putrescine + Urea [C]

General reaction: Amidine hydrolysis [C]

Inhibitor: EDTA; EGTA [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2377617 [H]