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The map label for this gene is thrS

Identifier: 212223522

GI number: 212223522

Start: 348859

End: 350742

Strand: Reverse

Name: thrS

Synonym: TON_0376

Alternate gene names: 212223522

Gene position: 350742-348859 (Counterclockwise)

Preceding gene: 212223528

Following gene: 212223517

Centisome position: 18.98

GC content: 54.09

Gene sequence:

>1884_bases
ATGAGAATGCTTCTAATACACAGCGACTATTTGGAGTACGAGGTCAAAGACAAGGCCTTGAAAAACCCAGAGCCAATAAG
CGAGGAGCAGAAGAAAGGCCGCCTGGAGGAGGTTCTGGCGGTTTTCATAAGCGTTGAAAAAGTCGATGAGACCAATCCCG
ATGAGGTTGTCGAGAAGGCCGTTAACGAAATCAAAGATGTTGCCTCCCAGGTTAAAGCCGAGAATGTCTTCGTCTATCCC
TTTGCCCACCTCAGCAGTGAGCTGGCCAAGCCAGACATTGCCCTGGAGGTTCTTAGGGAGGTCGAAGAGAAGCTCAGGGA
GGAGGGCTTCAATGTCAAGCGCGCCCCCTTCGGCTACTACAAGGCCTTCAAGCTCTCTTGTAAGGGCCACCCACTGGCAG
AACTCAGCAGGACGATAGTCCCGAGCGGCGAAGCCAAGGCCGAGGAGGAGATTCCAGAGGCCCTCAAGAAAGAGGAGGAA
GAACTCGTCAGCTACTGGTACATCCTAACCCCCGAAGGTGAGCTCATAGAGGTTGACAAGTTCGACTTCACCGGCCACGA
GAACCTCAGAAAGTTCGCCAACTACGAGATAAGCAAGAGCCGTATCGCTGACAGGGAGCCACCACACGTCAGGATAATGC
TCGAGCAGGAGCTCGTTGACTACGAACCAGGAAGCGACCCCGGAAACCTACGCTACTACCCGAAGGGCAGGCTCATCAAG
GGACTGCTGGAGCAGTACGTCACGGAGAAGGTCGTTGAGTACGGGGCGATGGAAGTCGAGACCCCGATTATGTACGACTT
CGAGCACCCAGCGCTTGAAAAGTACCTCAACCGCTTCCCGGCGAGGCAGTACATCGTTAAGAGCGGTGACAAGAAGTTCT
TCCTCCGCTTTGCTGCCTGTTTCGGCCAGTTCCTCATCAAGAAGGATGCCATTATAAGCTACCGCAACCTGCCGTTGAGG
ATGTATGAACTGACTAGATACTCTTTCAGGCGCGAGAAGAGCGGTGAGCTTTCCGGCCTCAGAAGACTTAGGGCCTTCAC
GATGCCCGATATGCACACCGTCGCCAGGGACCTCAAACAGGCCATGGCCGAGTTCAAGAAGCAGTACAAACTCAGTATGG
AAGTTCTTAAGGGCGTTGGACTAACTCCAGAGGACTACGAGGTGGCCATAAGGTTCACCGAAGACTTCTGGAACGAGAAC
AGAGATTTCATCGTCGAGCTGGCAAAGATAATAGGCAAGCCCGTCCTGATAGAGATGTGGAAGCAGAGGTTCTTCTACTT
CATACTCAAGTTTGAGTTCAACTTCGTGGACAACCTCGACAAGGCTGCTGCCCTTAGCACCGTCCAGATAGACGTTGAGA
ACGCCGAAAGGTTCGGCATAACCTACTACGACGAGGACGGCAAGGAGAAGTACCCGCTCATCCTTCACTGCTCGCCGAGC
GGCGCCATCGAGAGGGTTATGTACGCAATCCTCGAGAAGCAGGCCAAGCTCCAGTCCAAGGGCATCAAACCGATGTTCCC
GCTCTGGCTCAGCCCGATACAGGTCCGTGTCATTCCTGTCAGCGAGGAAGTTCTCGACTACGCACTCTACGTTGCAGGAA
AGCTCGAGGGTGCCAAGATTCGCGTCGACGTTGATGACACCTCCGACAGGCTCAACAAGAAGATAAGAAAGGCCGAGAAG
GAGTGGATTCCCTACGTTATCGTCGTCGGCAGAAACGAGAAGGAGCAGAACACGATAACCGTCAGAAGAAGGAGCGACGG
AAAGCAGGGTGAGATGCAGCTCGAGGACCTCATAAGGGAGATAAGGAGCCAGACTGAGGGCTTCCCCTACAAGCCGAGGC
CGCTGCCGCTTCTCCTCTCGAAGAGGCCCAAGTTCAGGGGCTAA

Upstream 100 bases:

>100_bases
TCCCGTCAGGATTTTTTAAGTCTTTGCCACGCGGGAGAATTTATAAAGCCAAGGGGAAAAGTTTTAGTAATTCCAATTGA
GCCTATGGTGGTGGTTAAGG

Downstream 100 bases:

>100_bases
TCTTTCGTACCTTTAGCTTTTCTATTCCTTTGTCTTTTGTGACTCTGAAGGAATAGGCCGTTGGCTCAAACTCTTCGTAT
GGAGCTGTTATCAGATAAAG

Product: threonyl-tRNA synthetase

Products: NA

Alternate protein names: Threonine--tRNA ligase; ThrRS

Number of amino acids: Translated: 627; Mature: 627

Protein sequence:

>627_residues
MRMLLIHSDYLEYEVKDKALKNPEPISEEQKKGRLEEVLAVFISVEKVDETNPDEVVEKAVNEIKDVASQVKAENVFVYP
FAHLSSELAKPDIALEVLREVEEKLREEGFNVKRAPFGYYKAFKLSCKGHPLAELSRTIVPSGEAKAEEEIPEALKKEEE
ELVSYWYILTPEGELIEVDKFDFTGHENLRKFANYEISKSRIADREPPHVRIMLEQELVDYEPGSDPGNLRYYPKGRLIK
GLLEQYVTEKVVEYGAMEVETPIMYDFEHPALEKYLNRFPARQYIVKSGDKKFFLRFAACFGQFLIKKDAIISYRNLPLR
MYELTRYSFRREKSGELSGLRRLRAFTMPDMHTVARDLKQAMAEFKKQYKLSMEVLKGVGLTPEDYEVAIRFTEDFWNEN
RDFIVELAKIIGKPVLIEMWKQRFFYFILKFEFNFVDNLDKAAALSTVQIDVENAERFGITYYDEDGKEKYPLILHCSPS
GAIERVMYAILEKQAKLQSKGIKPMFPLWLSPIQVRVIPVSEEVLDYALYVAGKLEGAKIRVDVDDTSDRLNKKIRKAEK
EWIPYVIVVGRNEKEQNTITVRRRSDGKQGEMQLEDLIREIRSQTEGFPYKPRPLPLLLSKRPKFRG

Sequences:

>Translated_627_residues
MRMLLIHSDYLEYEVKDKALKNPEPISEEQKKGRLEEVLAVFISVEKVDETNPDEVVEKAVNEIKDVASQVKAENVFVYP
FAHLSSELAKPDIALEVLREVEEKLREEGFNVKRAPFGYYKAFKLSCKGHPLAELSRTIVPSGEAKAEEEIPEALKKEEE
ELVSYWYILTPEGELIEVDKFDFTGHENLRKFANYEISKSRIADREPPHVRIMLEQELVDYEPGSDPGNLRYYPKGRLIK
GLLEQYVTEKVVEYGAMEVETPIMYDFEHPALEKYLNRFPARQYIVKSGDKKFFLRFAACFGQFLIKKDAIISYRNLPLR
MYELTRYSFRREKSGELSGLRRLRAFTMPDMHTVARDLKQAMAEFKKQYKLSMEVLKGVGLTPEDYEVAIRFTEDFWNEN
RDFIVELAKIIGKPVLIEMWKQRFFYFILKFEFNFVDNLDKAAALSTVQIDVENAERFGITYYDEDGKEKYPLILHCSPS
GAIERVMYAILEKQAKLQSKGIKPMFPLWLSPIQVRVIPVSEEVLDYALYVAGKLEGAKIRVDVDDTSDRLNKKIRKAEK
EWIPYVIVVGRNEKEQNTITVRRRSDGKQGEMQLEDLIREIRSQTEGFPYKPRPLPLLLSKRPKFRG
>Mature_627_residues
MRMLLIHSDYLEYEVKDKALKNPEPISEEQKKGRLEEVLAVFISVEKVDETNPDEVVEKAVNEIKDVASQVKAENVFVYP
FAHLSSELAKPDIALEVLREVEEKLREEGFNVKRAPFGYYKAFKLSCKGHPLAELSRTIVPSGEAKAEEEIPEALKKEEE
ELVSYWYILTPEGELIEVDKFDFTGHENLRKFANYEISKSRIADREPPHVRIMLEQELVDYEPGSDPGNLRYYPKGRLIK
GLLEQYVTEKVVEYGAMEVETPIMYDFEHPALEKYLNRFPARQYIVKSGDKKFFLRFAACFGQFLIKKDAIISYRNLPLR
MYELTRYSFRREKSGELSGLRRLRAFTMPDMHTVARDLKQAMAEFKKQYKLSMEVLKGVGLTPEDYEVAIRFTEDFWNEN
RDFIVELAKIIGKPVLIEMWKQRFFYFILKFEFNFVDNLDKAAALSTVQIDVENAERFGITYYDEDGKEKYPLILHCSPS
GAIERVMYAILEKQAKLQSKGIKPMFPLWLSPIQVRVIPVSEEVLDYALYVAGKLEGAKIRVDVDDTSDRLNKKIRKAEK
EWIPYVIVVGRNEKEQNTITVRRRSDGKQGEMQLEDLIREIRSQTEGFPYKPRPLPLLLSKRPKFRG

Specific function: Thrs Is Also A Translational Repressor Protein, It Controls The Translation Of Its Own Gene By Binding To Its mRNA. [C]

COG id: COG0441

COG function: function code J; Threonyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI38570111, Length=408, Percent_Identity=28.921568627451, Blast_Score=140, Evalue=4e-33,
Organism=Homo sapiens, GI38202255, Length=412, Percent_Identity=27.1844660194175, Blast_Score=129, Evalue=1e-29,
Organism=Homo sapiens, GI20070344, Length=418, Percent_Identity=26.7942583732057, Blast_Score=124, Evalue=3e-28,
Organism=Escherichia coli, GI1788013, Length=389, Percent_Identity=27.7634961439589, Blast_Score=144, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI71984192, Length=410, Percent_Identity=27.3170731707317, Blast_Score=143, Evalue=4e-34,
Organism=Caenorhabditis elegans, GI71984184, Length=410, Percent_Identity=27.3170731707317, Blast_Score=142, Evalue=4e-34,
Organism=Saccharomyces cerevisiae, GI6322112, Length=411, Percent_Identity=27.007299270073, Blast_Score=115, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6322654, Length=412, Percent_Identity=25.7281553398058, Blast_Score=107, Evalue=6e-24,
Organism=Drosophila melanogaster, GI24583842, Length=410, Percent_Identity=29.5121951219512, Blast_Score=157, Evalue=3e-38,
Organism=Drosophila melanogaster, GI28574141, Length=410, Percent_Identity=29.5121951219512, Blast_Score=157, Evalue=3e-38,
Organism=Drosophila melanogaster, GI24583839, Length=410, Percent_Identity=29.5121951219512, Blast_Score=156, Evalue=4e-38,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYT_THEON (B6YTH4)

Other databases:

- EMBL:   CP000855
- RefSeq:   YP_002306758.1
- GeneID:   7016671
- GenomeReviews:   CP000855_GR
- KEGG:   ton:TON_0376
- HOGENOM:   HBG392335
- OMA:   ETPVMYD
- ProtClustDB:   PRK03991
- GO:   GO:0005737
- HAMAP:   MF_00184_A
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002320
- InterPro:   IPR015011
- Gene3D:   G3DSA:3.40.50.800
- PRINTS:   PR01047
- ProDom:   PD016189
- TIGRFAMs:   TIGR00418

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF08915 tRNA-Thr_ED; SSF52954 Anticodon_bd

EC number: =6.1.1.3

Molecular weight: Translated: 73068; Mature: 73068

Theoretical pI: Translated: 6.21; Mature: 6.21

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRMLLIHSDYLEYEVKDKALKNPEPISEEQKKGRLEEVLAVFISVEKVDETNPDEVVEKA
CEEEEEECCCHHEEEHHHHCCCCCCCCHHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHH
VNEIKDVASQVKAENVFVYPFAHLSSELAKPDIALEVLREVEEKLREEGFNVKRAPFGYY
HHHHHHHHHHHHHCCEEEEEHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCE
KAFKLSCKGHPLAELSRTIVPSGEAKAEEEIPEALKKEEEELVSYWYILTPEGELIEVDK
EEEEEECCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEECCCCCEEEEEC
FDFTGHENLRKFANYEISKSRIADREPPHVRIMLEQELVDYEPGSDPGNLRYYPKGRLIK
CCCCCHHHHHHHHCCCCCHHHCCCCCCCEEEEEEECHHHCCCCCCCCCCEEECCCCHHHH
GLLEQYVTEKVVEYGAMEVETPIMYDFEHPALEKYLNRFPARQYIVKSGDKKFFLRFAAC
HHHHHHHHHHHHHHCCEEECCCEEECCCCHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHH
FGQFLIKKDAIISYRNLPLRMYELTRYSFRREKSGELSGLRRLRAFTMPDMHTVARDLKQ
HHHHHHHHCHHEEECCCCEEHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHH
AMAEFKKQYKLSMEVLKGVGLTPEDYEVAIRFTEDFWNENRDFIVELAKIIGKPVLIEMW
HHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEHHHHCCCHHHHHHHHHHHCCHHHHHHH
KQRFFYFILKFEFNFVDNLDKAAALSTVQIDVENAERFGITYYDEDGKEKYPLILHCSPS
HHHHEEEEEEEEECHHCCCHHHHHEEEEEEEECCCCEECEEEECCCCCCCCCEEEEECCC
GAIERVMYAILEKQAKLQSKGIKPMFPLWLSPIQVRVIPVSEEVLDYALYVAGKLEGAKI
HHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCEEEEEEECHHHHHHHHHHHHCCCCCCEE
RVDVDDTSDRLNKKIRKAEKEWIPYVIVVGRNEKEQNTITVRRRSDGKQGEMQLEDLIRE
EEECCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEECCCCCCCCHHHHHHHHH
IRSQTEGFPYKPRPLPLLLSKRPKFRG
HHHHCCCCCCCCCCCCHHCCCCCCCCC
>Mature Secondary Structure
MRMLLIHSDYLEYEVKDKALKNPEPISEEQKKGRLEEVLAVFISVEKVDETNPDEVVEKA
CEEEEEECCCHHEEEHHHHCCCCCCCCHHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHH
VNEIKDVASQVKAENVFVYPFAHLSSELAKPDIALEVLREVEEKLREEGFNVKRAPFGYY
HHHHHHHHHHHHHCCEEEEEHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCE
KAFKLSCKGHPLAELSRTIVPSGEAKAEEEIPEALKKEEEELVSYWYILTPEGELIEVDK
EEEEEECCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEECCCCCEEEEEC
FDFTGHENLRKFANYEISKSRIADREPPHVRIMLEQELVDYEPGSDPGNLRYYPKGRLIK
CCCCCHHHHHHHHCCCCCHHHCCCCCCCEEEEEEECHHHCCCCCCCCCCEEECCCCHHHH
GLLEQYVTEKVVEYGAMEVETPIMYDFEHPALEKYLNRFPARQYIVKSGDKKFFLRFAAC
HHHHHHHHHHHHHHCCEEECCCEEECCCCHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHH
FGQFLIKKDAIISYRNLPLRMYELTRYSFRREKSGELSGLRRLRAFTMPDMHTVARDLKQ
HHHHHHHHCHHEEECCCCEEHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHH
AMAEFKKQYKLSMEVLKGVGLTPEDYEVAIRFTEDFWNENRDFIVELAKIIGKPVLIEMW
HHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEHHHHCCCHHHHHHHHHHHCCHHHHHHH
KQRFFYFILKFEFNFVDNLDKAAALSTVQIDVENAERFGITYYDEDGKEKYPLILHCSPS
HHHHEEEEEEEEECHHCCCHHHHHEEEEEEEECCCCEECEEEECCCCCCCCCEEEEECCC
GAIERVMYAILEKQAKLQSKGIKPMFPLWLSPIQVRVIPVSEEVLDYALYVAGKLEGAKI
HHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCEEEEEEECHHHHHHHHHHHHCCCCCCEE
RVDVDDTSDRLNKKIRKAEKEWIPYVIVVGRNEKEQNTITVRRRSDGKQGEMQLEDLIRE
EEECCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEECCCCCCCCHHHHHHHHH
IRSQTEGFPYKPRPLPLLLSKRPKFRG
HHHHCCCCCCCCCCCCHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA