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The map label for this gene is rnz

Identifier: 212223398

GI number: 212223398

Start: 224445

End: 225380

Strand: Reverse

Name: rnz

Synonym: TON_0252

Alternate gene names: 212223398

Gene position: 225380-224445 (Counterclockwise)

Preceding gene: 212223400

Following gene: 212223397

Centisome position: 12.2

GC content: 52.78

Gene sequence:

>936_bases
ATGCTTGAAGTGATATTCCTTGGCACCGGTGGTATAATGCCTAACCGCGAGAGGAACGTCCCGGCCATAGCGCTCCGCTA
CAAAGGTGAGATTATCCTCTTCGATGTTGGAGAAGGCACGATGAGGCAGATGAGCACTGCCAAGCTCAGCCCCATGAAAG
TCGAGAAGATATTCATCACGCACTTCCACGGCGACCACTACCTCGGCTTGGCCGCGCTAATCCAGACCATGAACCTTTGG
AACAGGGAAAAGCCTCTCCACATCTACGGACCCAAGTACACCTTCCGGTTCATCCAGAATTTTCTCAACAGCGGCTTCTT
CAGGCCAGGCTTTGATATACACGTCCATGAGATAGGTGAGGTAAGGCTCAAGTTTGGTGATTACGAAATCTGGAGCTTCA
AAGTTGAGCACGGAATTCCTGCTCTGGGCTATGTCTTCAAGGAAAAGGATAAGCGTGGGAAGTTCCTCCCCGAGAAGCTC
GCAGAATACGGCCTGAGCGAGGGGCCAATTCTGGGGAAGCTTGAGAAGCAGGGTCAAATCGAGTGGAACGGTCGAATAAT
CCGCCTGGAGGACGTGACCGGACCGAGGAGGAAGGGTGTTAAGGTGGTTTACACCGGCGATACCGAGCCGTGCGAGAGGA
CGAGGCTCTTCGCCGAGAACGCTGACCTGCTCATCCACGAGGCGACCTATCTGAGGCCGGAGGATAGAGGGGACAGTTAC
CACACCACCGTTGGGGAGGCCTGTGAGATAGCCAAAAAGGCCAAGGTTAAACTTTTGGCGTTGTTCCACAGAGCGTTCCG
TTATACTTATGACGAATACATGGCAAAGGCCAGAGAATTGTGCGATGTCCCCTTTGTTATCCCTAAGGACTTCGACGTTC
TGACTTTCAAATCCGGACGCTGGGAGATGAGAAACCTTCTGGAGGATTGGCAATGA

Upstream 100 bases:

>100_bases
AATTTCTTCCATTCCCAAACACCTCATACGGATTAACACTCTCAGGTATTTAACGCTTTGGCATGCTTAGGTTTTTAATC
CCAGAATGGAACATTAGACC

Downstream 100 bases:

>100_bases
GCTACCTGCGCTACGTCAAGCTTATCGGAACCATGCATGTCTCCCCAAAGAGTAGAGAAGAGGTAATAAGTACCATCCTT
TCTGAGAAACCTCACGCAGT

Product: ribonuclease Z

Products: NA

Alternate protein names: RNase Z; tRNA 3 endonuclease; tRNase Z

Number of amino acids: Translated: 311; Mature: 311

Protein sequence:

>311_residues
MLEVIFLGTGGIMPNRERNVPAIALRYKGEIILFDVGEGTMRQMSTAKLSPMKVEKIFITHFHGDHYLGLAALIQTMNLW
NREKPLHIYGPKYTFRFIQNFLNSGFFRPGFDIHVHEIGEVRLKFGDYEIWSFKVEHGIPALGYVFKEKDKRGKFLPEKL
AEYGLSEGPILGKLEKQGQIEWNGRIIRLEDVTGPRRKGVKVVYTGDTEPCERTRLFAENADLLIHEATYLRPEDRGDSY
HTTVGEACEIAKKAKVKLLALFHRAFRYTYDEYMAKARELCDVPFVIPKDFDVLTFKSGRWEMRNLLEDWQ

Sequences:

>Translated_311_residues
MLEVIFLGTGGIMPNRERNVPAIALRYKGEIILFDVGEGTMRQMSTAKLSPMKVEKIFITHFHGDHYLGLAALIQTMNLW
NREKPLHIYGPKYTFRFIQNFLNSGFFRPGFDIHVHEIGEVRLKFGDYEIWSFKVEHGIPALGYVFKEKDKRGKFLPEKL
AEYGLSEGPILGKLEKQGQIEWNGRIIRLEDVTGPRRKGVKVVYTGDTEPCERTRLFAENADLLIHEATYLRPEDRGDSY
HTTVGEACEIAKKAKVKLLALFHRAFRYTYDEYMAKARELCDVPFVIPKDFDVLTFKSGRWEMRNLLEDWQ
>Mature_311_residues
MLEVIFLGTGGIMPNRERNVPAIALRYKGEIILFDVGEGTMRQMSTAKLSPMKVEKIFITHFHGDHYLGLAALIQTMNLW
NREKPLHIYGPKYTFRFIQNFLNSGFFRPGFDIHVHEIGEVRLKFGDYEIWSFKVEHGIPALGYVFKEKDKRGKFLPEKL
AEYGLSEGPILGKLEKQGQIEWNGRIIRLEDVTGPRRKGVKVVYTGDTEPCERTRLFAENADLLIHEATYLRPEDRGDSY
HTTVGEACEIAKKAKVKLLALFHRAFRYTYDEYMAKARELCDVPFVIPKDFDVLTFKSGRWEMRNLLEDWQ

Specific function: Zinc phosphodiesterase, which displays some tRNA 3'- processing endonuclease activity. Probably involved in tRNA maturation, by removing a 3'-trailer from precursor tRNA

COG id: COG1234

COG function: function code R; Metal-dependent hydrolases of the beta-lactamase superfamily III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase Z family

Homologues:

Organism=Homo sapiens, GI8922122, Length=327, Percent_Identity=31.1926605504587, Blast_Score=125, Evalue=5e-29,
Organism=Escherichia coli, GI87082078, Length=275, Percent_Identity=32.7272727272727, Blast_Score=141, Evalue=6e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNZ_THEON (B6YT50)

Other databases:

- EMBL:   CP000855
- RefSeq:   YP_002306634.1
- ProteinModelPortal:   B6YT50
- GeneID:   7017914
- GenomeReviews:   CP000855_GR
- KEGG:   ton:TON_0252
- HOGENOM:   HBG648406
- OMA:   HSTSTMA
- ProtClustDB:   PRK00055
- HAMAP:   MF_01818
- InterPro:   IPR001279
- InterPro:   IPR013471
- SMART:   SM00849
- TIGRFAMs:   TIGR02651

Pfam domain/function: PF00753 Lactamase_B

EC number: =3.1.26.11

Molecular weight: Translated: 36085; Mature: 36085

Theoretical pI: Translated: 8.69; Mature: 8.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEVIFLGTGGIMPNRERNVPAIALRYKGEIILFDVGEGTMRQMSTAKLSPMKVEKIFIT
CEEEEEEECCCCCCCCCCCCCEEEEEECCEEEEEECCCCHHHHHHHCCCCCEEEEEEEEE
HFHGDHYLGLAALIQTMNLWNREKPLHIYGPKYTFRFIQNFLNSGFFRPGFDIHVHEIGE
EECCCCHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHCCCCCCCCEEEEEEECE
VRLKFGDYEIWSFKVEHGIPALGYVFKEKDKRGKFLPEKLAEYGLSEGPILGKLEKQGQI
EEEEECCEEEEEEEECCCCCHHHHHHHCCCCCCCCCHHHHHHCCCCCCCEEEECCCCCEE
EWNGRIIRLEDVTGPRRKGVKVVYTGDTEPCERTRLFAENADLLIHEATYLRPEDRGDSY
EECCEEEEEECCCCCHHCCEEEEECCCCCHHHHHHHHHCCCCEEEEEEEECCCCCCCCCC
HTTVGEACEIAKKAKVKLLALFHRAFRYTYDEYMAKARELCDVPFVIPKDFDVLTFKSGR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCEEEEEECCC
WEMRNLLEDWQ
HHHHHHHHHCC
>Mature Secondary Structure
MLEVIFLGTGGIMPNRERNVPAIALRYKGEIILFDVGEGTMRQMSTAKLSPMKVEKIFIT
CEEEEEEECCCCCCCCCCCCCEEEEEECCEEEEEECCCCHHHHHHHCCCCCEEEEEEEEE
HFHGDHYLGLAALIQTMNLWNREKPLHIYGPKYTFRFIQNFLNSGFFRPGFDIHVHEIGE
EECCCCHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHCCCCCCCCEEEEEEECE
VRLKFGDYEIWSFKVEHGIPALGYVFKEKDKRGKFLPEKLAEYGLSEGPILGKLEKQGQI
EEEEECCEEEEEEEECCCCCHHHHHHHCCCCCCCCCHHHHHHCCCCCCCEEEECCCCCEE
EWNGRIIRLEDVTGPRRKGVKVVYTGDTEPCERTRLFAENADLLIHEATYLRPEDRGDSY
EECCEEEEEECCCCCHHCCEEEEECCCCCHHHHHHHHHCCCCEEEEEEEECCCCCCCCCC
HTTVGEACEIAKKAKVKLLALFHRAFRYTYDEYMAKARELCDVPFVIPKDFDVLTFKSGR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCEEEEEECCC
WEMRNLLEDWQ
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA