Click here to switch to the map view.

The map label for this gene is pckG [H]

Identifier: 212223338

GI number: 212223338

Start: 169996

End: 171873

Strand: Reverse

Name: pckG [H]

Synonym: TON_0192

Alternate gene names: 212223338

Gene position: 171873-169996 (Counterclockwise)

Preceding gene: 212223343

Following gene: 212223337

Centisome position: 9.3

GC content: 53.35

Gene sequence:

>1878_bases
ATGAATGCCCTCGAAAGGCTTGAAAAGCTCCTCGATAAAGAGCAATTTGAGAAGATTAAAGCCATCGACAATCCAGAGCT
TCACAAGTTTTTGGTGGAGTGGATTGAGTGGCTTGAACCAAGCAAGGTTTTCGTCTGTACTGACAACGAAGAGGACGAGT
TCTACGTCCGCTGGAAGGCGCTCTACTACGGTGAGGAAAAGATTCTCGAGACACCGAACCACACCGTCCATTACGACAAC
TACTACGACCAGGCCCGCGACAAGGCCAATACTAAGCTCCTCGTTCCTGGTGGAAAGAAGATTCCCTTCCTCAACACTAA
GGATAGGGACGAGGGGTTGAAGGAAATCCGCGAGCTGATGAAAGGAGTTATGAGGGGTAAGGAGCTCTTCGTGTGCTTCT
TCGTCCTTGGGCCGAAGAACTCGGTCTTCACGATTCCTGCCGTCCAGCTCACGGATTCTGCTTATGTAGCCCACTCAGAG
TTCATCCTTTACAGGAAGGGCTATGAAGAGTTTAAACGTCTCGGAAGAAGCGCACGCTTCTTCAAGTTTGTCCACTCAGC
TGGGGAGCTCGACGAGAGGAAGACGAGCAAGAACCTCGATAAGAGGCGTATCTACATCGACCTAGTTGATGAGACCGTTT
ACTCTGTCAACACCCAGTACGGCGGTAACACGATAGGCCTTAAGAAGCTCGCTTTCAGGCTCACCATCCAGCGCGCCGTT
AGAGAGGGCTGGCTCAGCGAACACATGTTCCTGATGGGAGTAAACGGTCCCAACGGCAGGAAGACCTACTTCACCGGCGC
TTATCCCAGCATGTGCGGAAAGACATCCACCGCCATGATATCCTGGGAGAACATCGTCGGCGATGATTTGACGTTCATCG
TACCCATGAACGGAATAGCCCGTGGAGCAAACGTCGAGAAGGGTGTCTTTGGCATAATCCAGGGCGTCAATCCCGAAGAC
GATCCGATAATCTGGCAGGTTCTCCATTCGCCCGTCGAGATAATCTTCTCCAACGTCCTCGTCAAGGACGGAAAGCCCTA
CTGGAACGAGATGGGCGTACCAATACCAGGCGAGGGTGAAAACCACAGCGGGGAGTGGTGGCGCGGAAAGAAAGACGCCG
AAGGAAACGAGATTCCGCCGAGCCACAAGAACGCTCGCTTCACAGTCAGCCTTGAGCACTTCCCGAATGTTGACCTTGAA
GCGCTTGAGGCCCCCTGCGGCGTCGAGGTTGGTGGCATGATCTTCGGAGGCAGGGACAAGGACACATGGCCGCCAGTCAG
GGAAGCCTTCGACTGGAAGCATGGCGTCATAACGATGGGTGCTGCTCTTGAGAGTGAGACAACCGCCGCAACCCTTGGAA
AGGAAGGAGTTAGAGCCTTCAACCCGATGGCTATACTCGACTTCATGAGTGTTCCCCTCGGCGAATACATCGAGAACTAC
CTTGAGTTCGAGAAGAAGCTCAGAAAAGCCCCCAAGATATTCGCGGTGAACTACTTCCTCCGCGATGAGGACGGCAACTG
GCTCAACCATAAGCTCGACAAGGCAGTCTGGCTCAAGTGGATGGAGCTCCGCGTTCACGGCGACGTCGATGCCATAGATA
CTCCCATAGGCTACATCCCGAAGTATGGGGACTTAGCAAGGCTCTTCAGGGAGGTTCTCAACAAGGACTACAGCAGGGAG
GACTACGAGAAGCAGTTCACCATTAGGGTTCCGGAACTTCTGGCGAAGATAGAACGTATCGAGAAGATCTACCGCGAACA
GGTTAAGGAAGCTCCGGAGGAGCTCTTCCAGGTTCTCGAGGAGGAGAGGAAGAGGCTGCTCGAGGCCAGGGAAAAGTACG
GTGACTATATCAGCCCCTTCCAGCTTGAGAAGGCATGA

Upstream 100 bases:

>100_bases
TGCGTACGGGGACATGACTGAACTCATAAATACACTGATGAACGAGGTTTACCAAATAAACTATTTAAGACATCGTTCAA
CTCTTTCTGGAGGCGATAAC

Downstream 100 bases:

>100_bases
GCCCTTCTCTTCTTTTCCTTCTCCGGGTGCACTTTTCTGGGTACTCCCAAAACCCTTTTTAACCCCACTCTCCACATAGT
ATCACCGAAAATGATACATA

Product: phosphoenolpyruvate carboxykinase

Products: NA

Alternate protein names: PEP carboxykinase; PEPCK; PckTk; Phosphoenolpyruvate carboxylase [H]

Number of amino acids: Translated: 625; Mature: 625

Protein sequence:

>625_residues
MNALERLEKLLDKEQFEKIKAIDNPELHKFLVEWIEWLEPSKVFVCTDNEEDEFYVRWKALYYGEEKILETPNHTVHYDN
YYDQARDKANTKLLVPGGKKIPFLNTKDRDEGLKEIRELMKGVMRGKELFVCFFVLGPKNSVFTIPAVQLTDSAYVAHSE
FILYRKGYEEFKRLGRSARFFKFVHSAGELDERKTSKNLDKRRIYIDLVDETVYSVNTQYGGNTIGLKKLAFRLTIQRAV
REGWLSEHMFLMGVNGPNGRKTYFTGAYPSMCGKTSTAMISWENIVGDDLTFIVPMNGIARGANVEKGVFGIIQGVNPED
DPIIWQVLHSPVEIIFSNVLVKDGKPYWNEMGVPIPGEGENHSGEWWRGKKDAEGNEIPPSHKNARFTVSLEHFPNVDLE
ALEAPCGVEVGGMIFGGRDKDTWPPVREAFDWKHGVITMGAALESETTAATLGKEGVRAFNPMAILDFMSVPLGEYIENY
LEFEKKLRKAPKIFAVNYFLRDEDGNWLNHKLDKAVWLKWMELRVHGDVDAIDTPIGYIPKYGDLARLFREVLNKDYSRE
DYEKQFTIRVPELLAKIERIEKIYREQVKEAPEELFQVLEEERKRLLEAREKYGDYISPFQLEKA

Sequences:

>Translated_625_residues
MNALERLEKLLDKEQFEKIKAIDNPELHKFLVEWIEWLEPSKVFVCTDNEEDEFYVRWKALYYGEEKILETPNHTVHYDN
YYDQARDKANTKLLVPGGKKIPFLNTKDRDEGLKEIRELMKGVMRGKELFVCFFVLGPKNSVFTIPAVQLTDSAYVAHSE
FILYRKGYEEFKRLGRSARFFKFVHSAGELDERKTSKNLDKRRIYIDLVDETVYSVNTQYGGNTIGLKKLAFRLTIQRAV
REGWLSEHMFLMGVNGPNGRKTYFTGAYPSMCGKTSTAMISWENIVGDDLTFIVPMNGIARGANVEKGVFGIIQGVNPED
DPIIWQVLHSPVEIIFSNVLVKDGKPYWNEMGVPIPGEGENHSGEWWRGKKDAEGNEIPPSHKNARFTVSLEHFPNVDLE
ALEAPCGVEVGGMIFGGRDKDTWPPVREAFDWKHGVITMGAALESETTAATLGKEGVRAFNPMAILDFMSVPLGEYIENY
LEFEKKLRKAPKIFAVNYFLRDEDGNWLNHKLDKAVWLKWMELRVHGDVDAIDTPIGYIPKYGDLARLFREVLNKDYSRE
DYEKQFTIRVPELLAKIERIEKIYREQVKEAPEELFQVLEEERKRLLEAREKYGDYISPFQLEKA
>Mature_625_residues
MNALERLEKLLDKEQFEKIKAIDNPELHKFLVEWIEWLEPSKVFVCTDNEEDEFYVRWKALYYGEEKILETPNHTVHYDN
YYDQARDKANTKLLVPGGKKIPFLNTKDRDEGLKEIRELMKGVMRGKELFVCFFVLGPKNSVFTIPAVQLTDSAYVAHSE
FILYRKGYEEFKRLGRSARFFKFVHSAGELDERKTSKNLDKRRIYIDLVDETVYSVNTQYGGNTIGLKKLAFRLTIQRAV
REGWLSEHMFLMGVNGPNGRKTYFTGAYPSMCGKTSTAMISWENIVGDDLTFIVPMNGIARGANVEKGVFGIIQGVNPED
DPIIWQVLHSPVEIIFSNVLVKDGKPYWNEMGVPIPGEGENHSGEWWRGKKDAEGNEIPPSHKNARFTVSLEHFPNVDLE
ALEAPCGVEVGGMIFGGRDKDTWPPVREAFDWKHGVITMGAALESETTAATLGKEGVRAFNPMAILDFMSVPLGEYIENY
LEFEKKLRKAPKIFAVNYFLRDEDGNWLNHKLDKAVWLKWMELRVHGDVDAIDTPIGYIPKYGDLARLFREVLNKDYSRE
DYEKQFTIRVPELLAKIERIEKIYREQVKEAPEELFQVLEEERKRLLEAREKYGDYISPFQLEKA

Specific function: Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle [H]

COG id: COG1274

COG function: function code C; Phosphoenolpyruvate carboxykinase (GTP)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoenolpyruvate carboxykinase [GTP] family [H]

Homologues:

Organism=Homo sapiens, GI66346721, Length=540, Percent_Identity=33.7037037037037, Blast_Score=247, Evalue=2e-65,
Organism=Homo sapiens, GI187281517, Length=596, Percent_Identity=30.7046979865772, Blast_Score=222, Evalue=9e-58,
Organism=Homo sapiens, GI66346723, Length=309, Percent_Identity=33.0097087378641, Blast_Score=139, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI17555492, Length=560, Percent_Identity=29.6428571428571, Blast_Score=214, Evalue=8e-56,
Organism=Caenorhabditis elegans, GI32564407, Length=487, Percent_Identity=31.006160164271, Blast_Score=207, Evalue=1e-53,
Organism=Caenorhabditis elegans, GI32564411, Length=557, Percent_Identity=28.5457809694794, Blast_Score=204, Evalue=1e-52,
Organism=Caenorhabditis elegans, GI71989645, Length=614, Percent_Identity=28.5016286644951, Blast_Score=202, Evalue=3e-52,
Organism=Caenorhabditis elegans, GI71989658, Length=614, Percent_Identity=28.5016286644951, Blast_Score=202, Evalue=4e-52,
Organism=Caenorhabditis elegans, GI71989653, Length=602, Percent_Identity=28.5714285714286, Blast_Score=197, Evalue=2e-50,
Organism=Caenorhabditis elegans, GI71989649, Length=491, Percent_Identity=28.7169042769857, Blast_Score=172, Evalue=4e-43,
Organism=Caenorhabditis elegans, GI32564409, Length=313, Percent_Identity=33.5463258785943, Blast_Score=148, Evalue=1e-35,
Organism=Drosophila melanogaster, GI24655085, Length=618, Percent_Identity=30.2588996763754, Blast_Score=245, Evalue=5e-65,
Organism=Drosophila melanogaster, GI24655088, Length=508, Percent_Identity=32.0866141732283, Blast_Score=241, Evalue=1e-63,
Organism=Drosophila melanogaster, GI24655082, Length=622, Percent_Identity=29.5819935691318, Blast_Score=221, Evalue=1e-57,
Organism=Drosophila melanogaster, GI161077226, Length=517, Percent_Identity=31.7214700193424, Blast_Score=217, Evalue=2e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018091
- InterPro:   IPR013035
- InterPro:   IPR008209
- InterPro:   IPR008210 [H]

Pfam domain/function: PF00821 PEPCK [H]

EC number: =4.1.1.32 [H]

Molecular weight: Translated: 72163; Mature: 72163

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNALERLEKLLDKEQFEKIKAIDNPELHKFLVEWIEWLEPSKVFVCTDNEEDEFYVRWKA
CCHHHHHHHHHCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCEEEEEEE
LYYGEEKILETPNHTVHYDNYYDQARDKANTKLLVPGGKKIPFLNTKDRDEGLKEIRELM
EEECCHHHHCCCCCEEEECCHHHHHHHCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHH
KGVMRGKELFVCFFVLGPKNSVFTIPAVQLTDSAYVAHSEFILYRKGYEEFKRLGRSARF
HHHHCCCEEEEEEEEECCCCCEEEEEEEEECCCEEEEHHEEEEECCCHHHHHHHCCHHHH
FKFVHSAGELDERKTSKNLDKRRIYIDLVDETVYSVNTQYGGNTIGLKKLAFRLTIQRAV
HHHHHHHCCHHHHHHHCCCCCEEEEEEEECCHHHEEECCCCCCCCHHHHHHHHHHHHHHH
REGWLSEHMFLMGVNGPNGRKTYFTGAYPSMCGKTSTAMISWENIVGDDLTFIVPMNGIA
HHHHHCCCEEEEECCCCCCCEEEECCCCHHHCCCCCEEEEEEHHHCCCCEEEEEECCCCC
RGANVEKGVFGIIQGVNPEDDPIIWQVLHSPVEIIFSNVLVKDGKPYWNEMGVPIPGEGE
CCCCCCCCHHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHEECCCCHHHHCCCCCCCCCC
NHSGEWWRGKKDAEGNEIPPSHKNARFTVSLEHFPNVDLEALEAPCGVEVGGMIFGGRDK
CCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHCCCCCCEECCEEECCCCC
DTWPPVREAFDWKHGVITMGAALESETTAATLGKEGVRAFNPMAILDFMSVPLGEYIENY
CCCCCHHHHCCCCCCEEEECCHHCCCHHHHHHCHHHHHHCCCHHHHHHHHCCHHHHHHHH
LEFEKKLRKAPKIFAVNYFLRDEDGNWLNHKLDKAVWLKWMELRVHGDVDAIDTPIGYIP
HHHHHHHHCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCC
KYGDLARLFREVLNKDYSREDYEKQFTIRVPELLAKIERIEKIYREQVKEAPEELFQVLE
CCHHHHHHHHHHHCCCCCCCCHHHEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EERKRLLEAREKYGDYISPFQLEKA
HHHHHHHHHHHHHCCCCCCEECCCC
>Mature Secondary Structure
MNALERLEKLLDKEQFEKIKAIDNPELHKFLVEWIEWLEPSKVFVCTDNEEDEFYVRWKA
CCHHHHHHHHHCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCEEEEEEE
LYYGEEKILETPNHTVHYDNYYDQARDKANTKLLVPGGKKIPFLNTKDRDEGLKEIRELM
EEECCHHHHCCCCCEEEECCHHHHHHHCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHH
KGVMRGKELFVCFFVLGPKNSVFTIPAVQLTDSAYVAHSEFILYRKGYEEFKRLGRSARF
HHHHCCCEEEEEEEEECCCCCEEEEEEEEECCCEEEEHHEEEEECCCHHHHHHHCCHHHH
FKFVHSAGELDERKTSKNLDKRRIYIDLVDETVYSVNTQYGGNTIGLKKLAFRLTIQRAV
HHHHHHHCCHHHHHHHCCCCCEEEEEEEECCHHHEEECCCCCCCCHHHHHHHHHHHHHHH
REGWLSEHMFLMGVNGPNGRKTYFTGAYPSMCGKTSTAMISWENIVGDDLTFIVPMNGIA
HHHHHCCCEEEEECCCCCCCEEEECCCCHHHCCCCCEEEEEEHHHCCCCEEEEEECCCCC
RGANVEKGVFGIIQGVNPEDDPIIWQVLHSPVEIIFSNVLVKDGKPYWNEMGVPIPGEGE
CCCCCCCCHHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHEECCCCHHHHCCCCCCCCCC
NHSGEWWRGKKDAEGNEIPPSHKNARFTVSLEHFPNVDLEALEAPCGVEVGGMIFGGRDK
CCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHCCCCCCEECCEEECCCCC
DTWPPVREAFDWKHGVITMGAALESETTAATLGKEGVRAFNPMAILDFMSVPLGEYIENY
CCCCCHHHHCCCCCCEEEECCHHCCCHHHHHHCHHHHHHCCCHHHHHHHHCCHHHHHHHH
LEFEKKLRKAPKIFAVNYFLRDEDGNWLNHKLDKAVWLKWMELRVHGDVDAIDTPIGYIP
HHHHHHHHCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCC
KYGDLARLFREVLNKDYSREDYEKQFTIRVPELLAKIERIEKIYREQVKEAPEELFQVLE
CCHHHHHHHHHHHCCCCCCCCHHHEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EERKRLLEAREKYGDYISPFQLEKA
HHHHHHHHHHHHHCCCCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA