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The map label for this gene is glmU

Identifier: 209696441

GI number: 209696441

Start: 3312114

End: 3313472

Strand: Reverse

Name: glmU

Synonym: VSAL_I3057

Alternate gene names: 209696441

Gene position: 3313472-3312114 (Counterclockwise)

Preceding gene: 209696442

Following gene: 209696439

Centisome position: 99.65

GC content: 41.21

Gene sequence:

>1359_bases
ATGAACTTCAGTGCAGTAATTCTTGCAGCGGGTAAAGGCACTCGCATGTATTCAAATAAACCTAAAGTACTTCATACTTT
AGCGGGTAAACCTATGGCGAAACATGTGATCGATACATGTGAAGGTTTAGGGGCTAAAAATATTCACCTTGTTTATGGTC
ACGGTGGTGATCAAATGCAAGCTGAGTTAGGCCAAGAGAGAGTAACTTGGGTACTTCAAGCTGAACAACTGGGTACAGGT
CATGCCGTTAATCAAGCATCACCAGAATTTTCTGATGATGAAAAAGTATTGATCCTTTATGGCGATGTTCCATTGATCAG
TACTGCAACGGTTGAAAACTTATTAGATGCTCAACCTACTGGTGGTATTGCTCTTTTGACTGTCGTTTTAGATAACCCGA
TGGGTTATGGTCGTATTATTCGTCGTAATGGCCCTGTCATTGCGATTGTAGAGCAAAAAGACGCGACAGAAGAGCAAAAG
CTCATTAAAGAGATTAACACCGGTGTGATGGTTGCTACGGGCGGTGATTTAAAGCGCTGGTTAGCGGCGTTGAAAAATGA
AAATGCACAAGGTGAGTATTATCTAACGGATATCATTGCTGCTGCTCATGATGAAGGTCGTGCAGTTGAAGCGGTACACC
CTGTTAGCCCTATTGAAGTTGAAGGGGTTAATGATCGTGCACAATTGGCTCGTTTAGAGAGAGCATATCAAGCAGCGCAA
GCTCAGAAATTGCTAGAGCAAGGCGTAATGCTTCGTGATCCTAGTCGTTTCGATCTTCGAGGATCATTGCAATGTGGTAT
GGATATTGAGATCGATGTTAATGTGATCATTGAAGGTAATGTTACCTTAGGTGACAATGTTGTCATTGGTGCTGGTTGTG
TACTTAAAGATTGTGAGATTGATGATAATACAATTATTCGTCCATACAGCGTGATTGAAGGCGCAACGGTTGGTGAGAAG
TGTACTGTTGGTCCATTTACTCGTTTACGTCCAGGTGCAGAATTGTGTAATGATGCGCATGTAGGTAATTTTGTTGAAGT
CAAAAATGTACGTTTAGGTGAAGGCTCAAAAGCAAATCATTTAACGTATTTGGGTGATGCAGAGATTGGTAAGCGTGTAA
ATGTTGGTGCTGGTGTTATTACTTGTAATTATGATGGTGCAAATAAATTTAAAACCATTATTGGTGATGATGTGTTTGTT
GGTTCAGATAGCCAATTAATTGCTCCTGTTACTATTGGTAATGGTGCAACGATTGGTGCGGGTTCTACGGTGACTAAAGA
TGTCGCCGAAAATGAATTGATGATCAGTCGTGCAAAAGAGCGCCGAATTGAACATTGGCAACGACCAACAAAAAAGTAA

Upstream 100 bases:

>100_bases
TTATGAATAATATTCATGAAGTAAATAATGGATTTAGTGTTTTTGGTATTTAATAAAGCTAGGCAAATTAAGTCAGCATA
AAAAATAGAATGGGCTTTTT

Downstream 100 bases:

>100_bases
GATGAGTTAATCAAATTAAGAAGAAGTCATTATGGCTTCTTTTTTTATGCCTATTTAAAAGGTAAGGAATTTGAATGTTG
CTGAGTTAGCATTGGCCCGT

Product: bifunctional N-acetylglucosamine-1-phosphate uridyltransferase/glucosamine-1-phosphate acetyltransferase

Products: NA

Alternate protein names: UDP-N-acetylglucosamine pyrophosphorylase; N-acetylglucosamine-1-phosphate uridyltransferase; Glucosamine-1-phosphate N-acetyltransferase

Number of amino acids: Translated: 452; Mature: 452

Protein sequence:

>452_residues
MNFSAVILAAGKGTRMYSNKPKVLHTLAGKPMAKHVIDTCEGLGAKNIHLVYGHGGDQMQAELGQERVTWVLQAEQLGTG
HAVNQASPEFSDDEKVLILYGDVPLISTATVENLLDAQPTGGIALLTVVLDNPMGYGRIIRRNGPVIAIVEQKDATEEQK
LIKEINTGVMVATGGDLKRWLAALKNENAQGEYYLTDIIAAAHDEGRAVEAVHPVSPIEVEGVNDRAQLARLERAYQAAQ
AQKLLEQGVMLRDPSRFDLRGSLQCGMDIEIDVNVIIEGNVTLGDNVVIGAGCVLKDCEIDDNTIIRPYSVIEGATVGEK
CTVGPFTRLRPGAELCNDAHVGNFVEVKNVRLGEGSKANHLTYLGDAEIGKRVNVGAGVITCNYDGANKFKTIIGDDVFV
GSDSQLIAPVTIGNGATIGAGSTVTKDVAENELMISRAKERRIEHWQRPTKK

Sequences:

>Translated_452_residues
MNFSAVILAAGKGTRMYSNKPKVLHTLAGKPMAKHVIDTCEGLGAKNIHLVYGHGGDQMQAELGQERVTWVLQAEQLGTG
HAVNQASPEFSDDEKVLILYGDVPLISTATVENLLDAQPTGGIALLTVVLDNPMGYGRIIRRNGPVIAIVEQKDATEEQK
LIKEINTGVMVATGGDLKRWLAALKNENAQGEYYLTDIIAAAHDEGRAVEAVHPVSPIEVEGVNDRAQLARLERAYQAAQ
AQKLLEQGVMLRDPSRFDLRGSLQCGMDIEIDVNVIIEGNVTLGDNVVIGAGCVLKDCEIDDNTIIRPYSVIEGATVGEK
CTVGPFTRLRPGAELCNDAHVGNFVEVKNVRLGEGSKANHLTYLGDAEIGKRVNVGAGVITCNYDGANKFKTIIGDDVFV
GSDSQLIAPVTIGNGATIGAGSTVTKDVAENELMISRAKERRIEHWQRPTKK
>Mature_452_residues
MNFSAVILAAGKGTRMYSNKPKVLHTLAGKPMAKHVIDTCEGLGAKNIHLVYGHGGDQMQAELGQERVTWVLQAEQLGTG
HAVNQASPEFSDDEKVLILYGDVPLISTATVENLLDAQPTGGIALLTVVLDNPMGYGRIIRRNGPVIAIVEQKDATEEQK
LIKEINTGVMVATGGDLKRWLAALKNENAQGEYYLTDIIAAAHDEGRAVEAVHPVSPIEVEGVNDRAQLARLERAYQAAQ
AQKLLEQGVMLRDPSRFDLRGSLQCGMDIEIDVNVIIEGNVTLGDNVVIGAGCVLKDCEIDDNTIIRPYSVIEGATVGEK
CTVGPFTRLRPGAELCNDAHVGNFVEVKNVRLGEGSKANHLTYLGDAEIGKRVNVGAGVITCNYDGANKFKTIIGDDVFV
GSDSQLIAPVTIGNGATIGAGSTVTKDVAENELMISRAKERRIEHWQRPTKK

Specific function: Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-GlcNAc. Responsible for the acetylation of Glc-N-1-P to give GlcNAc-1-P and for the uridyl transfer from UTP to GlcNAc-1-P which produces UDP-GlcNAc

COG id: COG1207

COG function: function code M; N-acetylglucosamine-1-phosphate uridyltransferase (contains nucleotidyltransferase and I-patch acetyltransferase domains)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transferase hexapeptide repeat family

Homologues:

Organism=Escherichia coli, GI1790168, Length=450, Percent_Identity=67.5555555555556, Blast_Score=631, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLMU_ALISL (B6EHG2)

Other databases:

- EMBL:   FM178379
- RefSeq:   YP_002264372.1
- ProteinModelPortal:   B6EHG2
- GeneID:   6988058
- GenomeReviews:   FM178379_GR
- KEGG:   vsa:VSAL_I3057
- HOGENOM:   HBG688195
- OMA:   GSKVNHL
- ProtClustDB:   PRK09451
- GO:   GO:0005737
- HAMAP:   MF_01631
- InterPro:   IPR005882
- InterPro:   IPR001451
- InterPro:   IPR018357
- InterPro:   IPR005835
- InterPro:   IPR011004
- TIGRFAMs:   TIGR01173

Pfam domain/function: PF00132 Hexapep; PF00483 NTP_transferase; SSF51161 Trimer_LpxA_like

EC number: =2.7.7.23; =2.3.1.157

Molecular weight: Translated: 48593; Mature: 48593

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: PS00101 HEXAPEP_TRANSFERASES

Important sites: ACT_SITE 360-360 BINDING 73-73 BINDING 137-137 BINDING 151-151 BINDING 166-166 BINDING 384-384 BINDING 402-402 BINDING 420-420 BINDING 437-437

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFSAVILAAGKGTRMYSNKPKVLHTLAGKPMAKHVIDTCEGLGAKNIHLVYGHGGDQMQ
CCCEEEEEECCCCCEEECCCCCEEEECCCCHHHHHHHHHHHCCCCCEEEEEECCCCCHHH
AELGQERVTWVLQAEQLGTGHAVNQASPEFSDDEKVLILYGDVPLISTATVENLLDAQPT
HHHCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEECCCCEEEHHHHHHHHCCCCC
GGIALLTVVLDNPMGYGRIIRRNGPVIAIVEQKDATEEQKLIKEINTGVMVATGGDLKRW
CCEEEEEEEECCCCCCCEEEECCCCEEEEECCCCCCHHHHHHHHHCCCEEEEECCHHHHH
LAALKNENAQGEYYLTDIIAAAHDEGRAVEAVHPVSPIEVEGVNDRAQLARLERAYQAAQ
HHHHHCCCCCCCEEEEEHHHHCCCCCCEEEEECCCCCEEEECCCHHHHHHHHHHHHHHHH
AQKLLEQGVMLRDPSRFDLRGSLQCGMDIEIDVNVIIEGNVTLGDNVVIGAGCVLKDCEI
HHHHHHCCCEEECCCCCCCCCCEECCEEEEEEEEEEEECCEEECCCEEEECCEEEEECCC
DDNTIIRPYSVIEGATVGEKCTVGPFTRLRPGAELCNDAHVGNFVEVKNVRLGEGSKANH
CCCCEECCHHHHCCCCCCCCCCCCCCHHCCCCHHHHCCCCCCCEEEEEEEEECCCCCCCC
LTYLGDAEIGKRVNVGAGVITCNYDGANKFKTIIGDDVFVGSDSQLIAPVTIGNGATIGA
EEEECCCCCCCEEECCCEEEEEECCCCCHHHHEECCEEEECCCCCEEEEEEECCCCEECC
GSTVTKDVAENELMISRAKERRIEHWQRPTKK
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MNFSAVILAAGKGTRMYSNKPKVLHTLAGKPMAKHVIDTCEGLGAKNIHLVYGHGGDQMQ
CCCEEEEEECCCCCEEECCCCCEEEECCCCHHHHHHHHHHHCCCCCEEEEEECCCCCHHH
AELGQERVTWVLQAEQLGTGHAVNQASPEFSDDEKVLILYGDVPLISTATVENLLDAQPT
HHHCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEECCCCEEEHHHHHHHHCCCCC
GGIALLTVVLDNPMGYGRIIRRNGPVIAIVEQKDATEEQKLIKEINTGVMVATGGDLKRW
CCEEEEEEEECCCCCCCEEEECCCCEEEEECCCCCCHHHHHHHHHCCCEEEEECCHHHHH
LAALKNENAQGEYYLTDIIAAAHDEGRAVEAVHPVSPIEVEGVNDRAQLARLERAYQAAQ
HHHHHCCCCCCCEEEEEHHHHCCCCCCEEEEECCCCCEEEECCCHHHHHHHHHHHHHHHH
AQKLLEQGVMLRDPSRFDLRGSLQCGMDIEIDVNVIIEGNVTLGDNVVIGAGCVLKDCEI
HHHHHHCCCEEECCCCCCCCCCEECCEEEEEEEEEEEECCEEECCCEEEECCEEEEECCC
DDNTIIRPYSVIEGATVGEKCTVGPFTRLRPGAELCNDAHVGNFVEVKNVRLGEGSKANH
CCCCEECCHHHHCCCCCCCCCCCCCCHHCCCCHHHHCCCCCCCEEEEEEEEECCCCCCCC
LTYLGDAEIGKRVNVGAGVITCNYDGANKFKTIIGDDVFVGSDSQLIAPVTIGNGATIGA
EEEECCCCCCCEEECCCEEEEEECCCCCHHHHEECCEEEECCCCCEEEEEEECCCCEECC
GSTVTKDVAENELMISRAKERRIEHWQRPTKK
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA