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The map label for this gene is fadA

Identifier: 209696367

GI number: 209696367

Start: 3219285

End: 3220448

Strand: Reverse

Name: fadA

Synonym: VSAL_I2973

Alternate gene names: 209696367

Gene position: 3220448-3219285 (Counterclockwise)

Preceding gene: 209696368

Following gene: 209696366

Centisome position: 96.85

GC content: 45.02

Gene sequence:

>1164_bases
ATGAAAAATGTCGTTATTGTTGATTGCATTCGAACACCGATGGGACGCTCTAAAAATGGGGTATTTCGTCATACTCGTGC
CGAAGATTTATCCGCGCACCTCATGAAAGGGTTATTAGAAAGAAACCCAAACGTTGACCCAAACCAAATTGAAGATATCT
ATTGGGGTTGTGTGCAGCAAACCTTAGAACAAGGCTTTAATATTGCTCGTAATGCATCGTTATTAGCAGGATTACCCAAA
TCCATTGCCGCAACAACAGTTAACCGCTTATGTGGTTCATCGATGCAGGCTCTACATGATGCCTCTCGTGCCATTATGGT
TGGAGATGCCGACATCTGCATTATTGGTGGTGTCGAGCACATGGGTCACGTACCAATGAACCATGGTGTTGATTTCCACT
CTGGCTTATCAAAAAACGTAGCTAAGGCTTCGGGTATGATGGGGTTAACGGCTGAAATGCTCGGTAAGATGCATGGTATC
TCTCGTGAACAACAAGATGCCTTTGCTTTTGCTTCTCATCAAAAAGCACATAGAGCGACGATTGAAGGGCACTTCGATTC
TGAGATTCTTCCGATGGAAGGTCACGATGAGAATGGGGCGCTAACGCTAGTGAAACACGATGAAGTCATTCGTCCAGAAA
CAACCCTTGAAGGTTTGGCTGCTCTGAGACCGGCTTTTGATCCCGCTAACGGTACCGTTACCGCAGGTAGTTCATCTGCA
TTATCGGATGGTGCTTCCGCCATGCTGATCATGAGCGAAGAAAAAGCAAATGAACTAGGATTAACCATTCGAGCGAAAAT
TCGTTCAATGGCGGTCTCTGGCTGCGATCCTGCCATTATGGGTTATGGACCTGTTCCTGCAACCAAAAAAGCATTAAAAC
GTGCCGGCCTATCCATTGATGATATTGACCTGTTTGAGCTGAACGAAGCTTTTGCCGCTCAATCACTGCCTTGCATTAAA
GACTTAGGTTTATTTGATGTGATGGAAGAAAAGATAAACTTAAATGGAGGGGCAATTGCGTTAGGTCATCCATTGGGTTG
CTCAGGTTCTCGTATTGCCACCACATTAATTAATAATATGGAGCGTACAGGTGCAAAACTGGGCGTAGCCACCATGTGTA
TTGGTTTAGGGCAAGGTATTGCAACGGTCTTTGAACGACCTTAG

Upstream 100 bases:

>100_bases
GTGCCTGACAGCATCAAACAAAAAGCAGCGGCTGGAGAGTGTTATTACCCAGCCCCGAAATCATCTGTTTCATCACCATC
GGTTTAAGGAAGAATTTATC

Downstream 100 bases:

>100_bases
GAATAGATTGATACGCTAGAAACTAAAAAAGTCGCCTTCTGGGCGACTTTTTTCGAGTTGATACCTTATTCGGTATCGCT
TGTTATCTTATTTTTTTAAG

Product: 3-ketoacyl-CoA thiolase

Products: NA

Alternate protein names: Acetyl-CoA acyltransferase; Beta-ketothiolase; Fatty acid oxidation complex subunit beta

Number of amino acids: Translated: 387; Mature: 387

Protein sequence:

>387_residues
MKNVVIVDCIRTPMGRSKNGVFRHTRAEDLSAHLMKGLLERNPNVDPNQIEDIYWGCVQQTLEQGFNIARNASLLAGLPK
SIAATTVNRLCGSSMQALHDASRAIMVGDADICIIGGVEHMGHVPMNHGVDFHSGLSKNVAKASGMMGLTAEMLGKMHGI
SREQQDAFAFASHQKAHRATIEGHFDSEILPMEGHDENGALTLVKHDEVIRPETTLEGLAALRPAFDPANGTVTAGSSSA
LSDGASAMLIMSEEKANELGLTIRAKIRSMAVSGCDPAIMGYGPVPATKKALKRAGLSIDDIDLFELNEAFAAQSLPCIK
DLGLFDVMEEKINLNGGAIALGHPLGCSGSRIATTLINNMERTGAKLGVATMCIGLGQGIATVFERP

Sequences:

>Translated_387_residues
MKNVVIVDCIRTPMGRSKNGVFRHTRAEDLSAHLMKGLLERNPNVDPNQIEDIYWGCVQQTLEQGFNIARNASLLAGLPK
SIAATTVNRLCGSSMQALHDASRAIMVGDADICIIGGVEHMGHVPMNHGVDFHSGLSKNVAKASGMMGLTAEMLGKMHGI
SREQQDAFAFASHQKAHRATIEGHFDSEILPMEGHDENGALTLVKHDEVIRPETTLEGLAALRPAFDPANGTVTAGSSSA
LSDGASAMLIMSEEKANELGLTIRAKIRSMAVSGCDPAIMGYGPVPATKKALKRAGLSIDDIDLFELNEAFAAQSLPCIK
DLGLFDVMEEKINLNGGAIALGHPLGCSGSRIATTLINNMERTGAKLGVATMCIGLGQGIATVFERP
>Mature_387_residues
MKNVVIVDCIRTPMGRSKNGVFRHTRAEDLSAHLMKGLLERNPNVDPNQIEDIYWGCVQQTLEQGFNIARNASLLAGLPK
SIAATTVNRLCGSSMQALHDASRAIMVGDADICIIGGVEHMGHVPMNHGVDFHSGLSKNVAKASGMMGLTAEMLGKMHGI
SREQQDAFAFASHQKAHRATIEGHFDSEILPMEGHDENGALTLVKHDEVIRPETTLEGLAALRPAFDPANGTVTAGSSSA
LSDGASAMLIMSEEKANELGLTIRAKIRSMAVSGCDPAIMGYGPVPATKKALKRAGLSIDDIDLFELNEAFAAQSLPCIK
DLGLFDVMEEKINLNGGAIALGHPLGCSGSRIATTLINNMERTGAKLGVATMCIGLGQGIATVFERP

Specific function: Catalyzes the final step of fatty acid oxidation in which acetyl-CoA is released and the CoA ester of a fatty acid two carbons shorter is formed

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family

Homologues:

Organism=Homo sapiens, GI4501853, Length=394, Percent_Identity=43.4010152284264, Blast_Score=284, Evalue=9e-77,
Organism=Homo sapiens, GI167614485, Length=398, Percent_Identity=40.7035175879397, Blast_Score=271, Evalue=1e-72,
Organism=Homo sapiens, GI148539872, Length=396, Percent_Identity=39.8989898989899, Blast_Score=257, Evalue=1e-68,
Organism=Homo sapiens, GI4504327, Length=431, Percent_Identity=34.5707656612529, Blast_Score=200, Evalue=2e-51,
Organism=Homo sapiens, GI4557237, Length=397, Percent_Identity=33.5012594458438, Blast_Score=200, Evalue=2e-51,
Organism=Homo sapiens, GI194353979, Length=390, Percent_Identity=32.8205128205128, Blast_Score=166, Evalue=3e-41,
Organism=Escherichia coli, GI48994986, Length=386, Percent_Identity=73.3160621761658, Blast_Score=593, Evalue=1e-171,
Organism=Escherichia coli, GI1787663, Length=404, Percent_Identity=45.7920792079208, Blast_Score=332, Evalue=2e-92,
Organism=Escherichia coli, GI87082165, Length=401, Percent_Identity=42.8927680798005, Blast_Score=287, Evalue=6e-79,
Organism=Escherichia coli, GI1788554, Length=400, Percent_Identity=45, Blast_Score=279, Evalue=2e-76,
Organism=Escherichia coli, GI1788683, Length=421, Percent_Identity=33.0166270783848, Blast_Score=185, Evalue=4e-48,
Organism=Caenorhabditis elegans, GI133906874, Length=400, Percent_Identity=37, Blast_Score=249, Evalue=2e-66,
Organism=Caenorhabditis elegans, GI17535921, Length=399, Percent_Identity=35.3383458646617, Blast_Score=204, Evalue=6e-53,
Organism=Caenorhabditis elegans, GI17551802, Length=431, Percent_Identity=34.8027842227378, Blast_Score=187, Evalue=1e-47,
Organism=Caenorhabditis elegans, GI25147385, Length=396, Percent_Identity=33.0808080808081, Blast_Score=183, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI17535917, Length=403, Percent_Identity=28.0397022332506, Blast_Score=123, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6322031, Length=391, Percent_Identity=38.618925831202, Blast_Score=238, Evalue=1e-63,
Organism=Saccharomyces cerevisiae, GI6325229, Length=402, Percent_Identity=36.318407960199, Blast_Score=219, Evalue=5e-58,
Organism=Drosophila melanogaster, GI24655093, Length=402, Percent_Identity=42.0398009950249, Blast_Score=281, Evalue=7e-76,
Organism=Drosophila melanogaster, GI17648125, Length=351, Percent_Identity=40.7407407407407, Blast_Score=234, Evalue=1e-61,
Organism=Drosophila melanogaster, GI17137578, Length=429, Percent_Identity=34.4988344988345, Blast_Score=199, Evalue=2e-51,
Organism=Drosophila melanogaster, GI24640423, Length=397, Percent_Identity=32.7455919395466, Blast_Score=187, Evalue=9e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FADA_ALISL (B6EGU1)

Other databases:

- EMBL:   FM178379
- RefSeq:   YP_002264298.1
- GeneID:   6988003
- GenomeReviews:   FM178379_GR
- KEGG:   vsa:VSAL_I2973
- HOGENOM:   HBG370930
- OMA:   MLVMEEE
- ProtClustDB:   PRK08947
- GO:   GO:0005737
- HAMAP:   MF_01620
- InterPro:   IPR012805
- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616
- Gene3D:   G3DSA:3.40.47.10
- PANTHER:   PTHR18919
- PIRSF:   PIRSF000429
- TIGRFAMs:   TIGR01930
- TIGRFAMs:   TIGR02445

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N; SSF53901 Thiolase-like

EC number: =2.3.1.16

Molecular weight: Translated: 41061; Mature: 41061

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: PS00098 THIOLASE_1; PS00737 THIOLASE_2; PS00099 THIOLASE_3

Important sites: ACT_SITE 91-91 ACT_SITE 343-343 ACT_SITE 373-373

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
7.0 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
7.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNVVIVDCIRTPMGRSKNGVFRHTRAEDLSAHLMKGLLERNPNVDPNQIEDIYWGCVQQ
CCCEEEEEECCCCCCCCCCCCEECCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
TLEQGFNIARNASLLAGLPKSIAATTVNRLCGSSMQALHDASRAIMVGDADICIIGGVEH
HHHHCCCHHCCCHHHHCCCHHHHHHHHHHHHCHHHHHHHHCCCEEEECCCCEEEECCHHH
MGHVPMNHGVDFHSGLSKNVAKASGMMGLTAEMLGKMHGISREQQDAFAFASHQKAHRAT
HCCCCCCCCCCHHCCCHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHE
IEGHFDSEILPMEGHDENGALTLVKHDEVIRPETTLEGLAALRPAFDPANGTVTAGSSSA
ECCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHCCCCCCCCCEEECCCCCC
LSDGASAMLIMSEEKANELGLTIRAKIRSMAVSGCDPAIMGYGPVPATKKALKRAGLSID
CCCCCCEEEEEECCCCCCCCEEHEEHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCC
DIDLFELNEAFAAQSLPCIKDLGLFDVMEEKINLNGGAIALGHPLGCSGSRIATTLINNM
CCHHHHHHHHHHHCCCCHHHHCCHHHHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHH
ERTGAKLGVATMCIGLGQGIATVFERP
HHHCHHHHHHHHHHHHCCHHHHHHCCC
>Mature Secondary Structure
MKNVVIVDCIRTPMGRSKNGVFRHTRAEDLSAHLMKGLLERNPNVDPNQIEDIYWGCVQQ
CCCEEEEEECCCCCCCCCCCCEECCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
TLEQGFNIARNASLLAGLPKSIAATTVNRLCGSSMQALHDASRAIMVGDADICIIGGVEH
HHHHCCCHHCCCHHHHCCCHHHHHHHHHHHHCHHHHHHHHCCCEEEECCCCEEEECCHHH
MGHVPMNHGVDFHSGLSKNVAKASGMMGLTAEMLGKMHGISREQQDAFAFASHQKAHRAT
HCCCCCCCCCCHHCCCHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHE
IEGHFDSEILPMEGHDENGALTLVKHDEVIRPETTLEGLAALRPAFDPANGTVTAGSSSA
ECCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHCCCCCCCCCEEECCCCCC
LSDGASAMLIMSEEKANELGLTIRAKIRSMAVSGCDPAIMGYGPVPATKKALKRAGLSID
CCCCCCEEEEEECCCCCCCCEEHEEHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCC
DIDLFELNEAFAAQSLPCIKDLGLFDVMEEKINLNGGAIALGHPLGCSGSRIATTLINNM
CCHHHHHHHHHHHCCCCHHHHCCHHHHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHH
ERTGAKLGVATMCIGLGQGIATVFERP
HHHCHHHHHHHHHHHHCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA