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The map label for this gene is btuB [H]

Identifier: 209696285

GI number: 209696285

Start: 3130386

End: 3132242

Strand: Reverse

Name: btuB [H]

Synonym: VSAL_I2886

Alternate gene names: 209696285

Gene position: 3132242-3130386 (Counterclockwise)

Preceding gene: 209696287

Following gene: 209696284

Centisome position: 94.2

GC content: 38.02

Gene sequence:

>1857_bases
ATGAAAAAAACTGCCCTTGCGACTGCAATAGTGTCGCTGCTTTCTCACGCCTATACCTCTCCTGCATTTGCAAAAGATTC
AGTATCTGAAACAGACGAAGTGATGGTGGTTACTGCCAATCGATTTGAGCAGTCGGCTAAAGATGTGATTGCACCAATGA
GTGTCGTTACAAAAGAAGATATTGTTCAGATTCAAGCTAAAACAATTACAGATATTCTCAGACTTCAACCAGGTATTGAG
GTCGCGAGTAATGGTGGCATTGGTCAGAATGCATCTATATTCATGAGAGGGACTAACTCTGATCATGTTCTTGTTTTAAT
CGACGGTTTTAGAATGAACAGTTCCATTAAAAGTGGAATGAATATTAATAAAATACCGGTTAATCAAATTGAGCGAATAG
AAATTATTCGAGGCTCAGGCGCTGTTATGTATGGTTCTGATGCGGTAGGTGGTGTAATTAATATTATTACTCGCACCGCT
ACGAATGATTTGTCAAAATCAGCAACGATTGGTGCAGGGAGTAATAAGTATAAAGAAGGAAACTTTGCGGCTAACACTAA
TGTTGGAGATAAAGGGCATTTAAAGATTGCTGCTGGCTTCACTCAAACGGAAGGGTATAACGTAAACCCGCAACCGGGAT
TGAATGATGGTGACAAACATGGTTTTGAAAATAACCAGTTTATGCTGAACTATGAACATCAATTTAGCTCAAACTTAAGT
TTATTCACCGGTATTCGTTGGTTCAACAGCACTTCTGAATACAATCATTATACAAACACAAAATCTCAGTCTGAATCCGA
ATCTATGGCGTACTCAGCAAAAGTCTCTTATAAAAAAGATGCGTTGTTATCTGATTTAAGTTTCAGTTACCAAGATAATG
AAACGTTAGAGTACCTTGAGAATGGGTCTTTAGATTCAGAACAAAAAATAGATCAACTTAATGCGCAATGGACAAACCAA
TATAAGTTAATGGATTCATGGTTTGTGAATGCAGGGATTGATTGGACAAATGAACAAGTAAAAGCTGATATTACTAACTT
TTCGCATCAAGTCATTATCGATGAAGGTGAGAGTCGAATGTCGACGGGTGTGTATTTAGGGACCGAGTATCAGCTAGAGA
AGTTAACCTTAGAAGCGAACACTCGATTTGATAAACATGATAAACATGATAAATACGATGAGTATGTAACTTGGGGAGTT
GGTGCAAGCTATCAGATTAACGAAATTTATCGTATCAGTAGTGCGTATAATACGGCATTTAAAGCCCCATCGTTCTATGA
TTTATCAACGGCACCTGATTTAGAACCAGAAACATCAGATAATATTGATGTTGGGTTAGCGGCTGAATATGCATTGGTAA
GTGTTAATTTGTCATTGTATAAAAACAAAGTAGATAATTTGATTATTTACTATGACGCACCAAACTACATGGGTTATAGC
GCTAATGTAGATGCGGATATCAAAGGTGCAGAACTGGAATTGCTATTTGATACTGGTATTGTGTCTCATACCGTGATTGC
TGAGTATAAAGATCACGAAGATACACAAGGACAACAGTTAGCACGAAGAGCGAAGCAAAACTATAAATGGATTAGTAACG
TGAGTTTCGGTGATTTAGATATGAACTTGTCTTATATATACACGGGGAAACGTTCTGATCTTCCTTCTGCCCCTGGTGTA
GCAACGAATTATTTAGATCCTTATTCTTTATGGACATTGGCGGGCGCTTATTGGGTAACTACCGAATTAGCAGTGCGTGG
TCGAGTGGAAAATTTGCTTGATGAAGAATATGAAACCGCGGGAGGGTACAAACCTGCAGAGCGTTCTTACTATGTCAGCT
TAGATTATCAATTTTAA

Upstream 100 bases:

>100_bases
CGCCTAAGTTCATTATTCTCAAGTCCGAAGACCTGCCAACAATAAGCAATACATGATGTATTGCTATATGGACTTAACGC
GATGTTAGGAAAACACGGAA

Downstream 100 bases:

>100_bases
GCATTATTATTAACCGCCTTTATTGGCGGTTTTTCTTTTCTGAGATTTATATGAAGAAGAAAGTTATTGTTAGTTGGTCA
TCAGGAAAAGATGCTACGTT

Product: outer membrane associated TonB dependent receptor

Products: NA

Alternate protein names: Cobalamin receptor; Outer membrane cobalamin translocator [H]

Number of amino acids: Translated: 618; Mature: 618

Protein sequence:

>618_residues
MKKTALATAIVSLLSHAYTSPAFAKDSVSETDEVMVVTANRFEQSAKDVIAPMSVVTKEDIVQIQAKTITDILRLQPGIE
VASNGGIGQNASIFMRGTNSDHVLVLIDGFRMNSSIKSGMNINKIPVNQIERIEIIRGSGAVMYGSDAVGGVINIITRTA
TNDLSKSATIGAGSNKYKEGNFAANTNVGDKGHLKIAAGFTQTEGYNVNPQPGLNDGDKHGFENNQFMLNYEHQFSSNLS
LFTGIRWFNSTSEYNHYTNTKSQSESESMAYSAKVSYKKDALLSDLSFSYQDNETLEYLENGSLDSEQKIDQLNAQWTNQ
YKLMDSWFVNAGIDWTNEQVKADITNFSHQVIIDEGESRMSTGVYLGTEYQLEKLTLEANTRFDKHDKHDKYDEYVTWGV
GASYQINEIYRISSAYNTAFKAPSFYDLSTAPDLEPETSDNIDVGLAAEYALVSVNLSLYKNKVDNLIIYYDAPNYMGYS
ANVDADIKGAELELLFDTGIVSHTVIAEYKDHEDTQGQQLARRAKQNYKWISNVSFGDLDMNLSYIYTGKRSDLPSAPGV
ATNYLDPYSLWTLAGAYWVTTELAVRGRVENLLDEEYETAGGYKPAERSYYVSLDYQF

Sequences:

>Translated_618_residues
MKKTALATAIVSLLSHAYTSPAFAKDSVSETDEVMVVTANRFEQSAKDVIAPMSVVTKEDIVQIQAKTITDILRLQPGIE
VASNGGIGQNASIFMRGTNSDHVLVLIDGFRMNSSIKSGMNINKIPVNQIERIEIIRGSGAVMYGSDAVGGVINIITRTA
TNDLSKSATIGAGSNKYKEGNFAANTNVGDKGHLKIAAGFTQTEGYNVNPQPGLNDGDKHGFENNQFMLNYEHQFSSNLS
LFTGIRWFNSTSEYNHYTNTKSQSESESMAYSAKVSYKKDALLSDLSFSYQDNETLEYLENGSLDSEQKIDQLNAQWTNQ
YKLMDSWFVNAGIDWTNEQVKADITNFSHQVIIDEGESRMSTGVYLGTEYQLEKLTLEANTRFDKHDKHDKYDEYVTWGV
GASYQINEIYRISSAYNTAFKAPSFYDLSTAPDLEPETSDNIDVGLAAEYALVSVNLSLYKNKVDNLIIYYDAPNYMGYS
ANVDADIKGAELELLFDTGIVSHTVIAEYKDHEDTQGQQLARRAKQNYKWISNVSFGDLDMNLSYIYTGKRSDLPSAPGV
ATNYLDPYSLWTLAGAYWVTTELAVRGRVENLLDEEYETAGGYKPAERSYYVSLDYQF
>Mature_618_residues
MKKTALATAIVSLLSHAYTSPAFAKDSVSETDEVMVVTANRFEQSAKDVIAPMSVVTKEDIVQIQAKTITDILRLQPGIE
VASNGGIGQNASIFMRGTNSDHVLVLIDGFRMNSSIKSGMNINKIPVNQIERIEIIRGSGAVMYGSDAVGGVINIITRTA
TNDLSKSATIGAGSNKYKEGNFAANTNVGDKGHLKIAAGFTQTEGYNVNPQPGLNDGDKHGFENNQFMLNYEHQFSSNLS
LFTGIRWFNSTSEYNHYTNTKSQSESESMAYSAKVSYKKDALLSDLSFSYQDNETLEYLENGSLDSEQKIDQLNAQWTNQ
YKLMDSWFVNAGIDWTNEQVKADITNFSHQVIIDEGESRMSTGVYLGTEYQLEKLTLEANTRFDKHDKHDKYDEYVTWGV
GASYQINEIYRISSAYNTAFKAPSFYDLSTAPDLEPETSDNIDVGLAAEYALVSVNLSLYKNKVDNLIIYYDAPNYMGYS
ANVDADIKGAELELLFDTGIVSHTVIAEYKDHEDTQGQQLARRAKQNYKWISNVSFGDLDMNLSYIYTGKRSDLPSAPGV
ATNYLDPYSLWTLAGAYWVTTELAVRGRVENLLDEEYETAGGYKPAERSYYVSLDYQF

Specific function: Involved in the active translocation of vitamin B12 (cyanocobalamin) across the outer membrane to the periplasmic space. It derives its energy for transport by interacting with the trans-periplasmic membrane protein TonB [H]

COG id: COG4206

COG function: function code H; Outer membrane cobalamin receptor protein

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TonB-dependent receptor family. BtuB (TC 1.B.14.3.1) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790405, Length=639, Percent_Identity=33.9593114241002, Blast_Score=305, Evalue=7e-84,
Organism=Escherichia coli, GI1788478, Length=680, Percent_Identity=24.5588235294118, Blast_Score=139, Evalue=4e-34,
Organism=Escherichia coli, GI1786798, Length=231, Percent_Identity=28.5714285714286, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1790743, Length=124, Percent_Identity=29.8387096774194, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1787024, Length=239, Percent_Identity=25.1046025104602, Blast_Score=63, Evalue=5e-11,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012910
- InterPro:   IPR000531
- InterPro:   IPR010916
- InterPro:   IPR010917 [H]

Pfam domain/function: PF07715 Plug; PF00593 TonB_dep_Rec [H]

EC number: NA

Molecular weight: Translated: 68804; Mature: 68804

Theoretical pI: Translated: 4.52; Mature: 4.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKTALATAIVSLLSHAYTSPAFAKDSVSETDEVMVVTANRFEQSAKDVIAPMSVVTKED
CCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHCHHH
IVQIQAKTITDILRLQPGIEVASNGGIGQNASIFMRGTNSDHVLVLIDGFRMNSSIKSGM
HHEEHHHHHHHHHHCCCCCEEECCCCCCCCCEEEEEECCCCEEEEEEECCEECCHHHCCC
NINKIPVNQIERIEIIRGSGAVMYGSDAVGGVINIITRTATNDLSKSATIGAGSNKYKEG
CCCCCCCCCCEEEEEEECCCEEEECCCCHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCC
NFAANTNVGDKGHLKIAAGFTQTEGYNVNPQPGLNDGDKHGFENNQFMLNYEHQFSSNLS
CEEECCCCCCCCCEEEEECCEECCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCE
LFTGIRWFNSTSEYNHYTNTKSQSESESMAYSAKVSYKKDALLSDLSFSYQDNETLEYLE
EEEEEEEECCCCCCCCCCCCCCCCCHHHEEEEEECCCCHHHHHHHCCCCCCCCCHHHHHH
NGSLDSEQKIDQLNAQWTNQYKLMDSWFVNAGIDWTNEQVKADITNFSHQVIIDEGESRM
CCCCCCHHHHHHHCCHHCCCEEEEEEEEEECCCCCCCCHHEEHHCCCCEEEEEECCCCCC
STGVYLGTEYQLEKLTLEANTRFDKHDKHDKYDEYVTWGVGASYQINEIYRISSAYNTAF
CCCEEECCCEEEEEEEEECCCCCCCCCCCCCCCCEEEECCCCCEEECCEEEEHHHHHHHC
KAPSFYDLSTAPDLEPETSDNIDVGLAAEYALVSVNLSLYKNKVDNLIIYYDAPNYMGYS
CCCCCEECCCCCCCCCCCCCCCEEEEEEEEEEEEEEHHHHHCCCCCEEEEECCCCCCCCC
ANVDADIKGAELELLFDTGIVSHTVIAEYKDHEDTQGQQLARRAKQNYKWISNVSFGDLD
CCCCCCCCCCEEEEEEECCCHHEEEEEECCCCCCCHHHHHHHHHHHCCEEECCCCCCCCC
MNLSYIYTGKRSDLPSAPGVATNYLDPYSLWTLAGAYWVTTELAVRGRVENLLDEEYETA
CCEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHCCEEEEEHHHHHHHHHHHHHHHHHHC
GGYKPAERSYYVSLDYQF
CCCCCCCCEEEEEEEECC
>Mature Secondary Structure
MKKTALATAIVSLLSHAYTSPAFAKDSVSETDEVMVVTANRFEQSAKDVIAPMSVVTKED
CCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHCHHH
IVQIQAKTITDILRLQPGIEVASNGGIGQNASIFMRGTNSDHVLVLIDGFRMNSSIKSGM
HHEEHHHHHHHHHHCCCCCEEECCCCCCCCCEEEEEECCCCEEEEEEECCEECCHHHCCC
NINKIPVNQIERIEIIRGSGAVMYGSDAVGGVINIITRTATNDLSKSATIGAGSNKYKEG
CCCCCCCCCCEEEEEEECCCEEEECCCCHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCC
NFAANTNVGDKGHLKIAAGFTQTEGYNVNPQPGLNDGDKHGFENNQFMLNYEHQFSSNLS
CEEECCCCCCCCCEEEEECCEECCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCE
LFTGIRWFNSTSEYNHYTNTKSQSESESMAYSAKVSYKKDALLSDLSFSYQDNETLEYLE
EEEEEEEECCCCCCCCCCCCCCCCCHHHEEEEEECCCCHHHHHHHCCCCCCCCCHHHHHH
NGSLDSEQKIDQLNAQWTNQYKLMDSWFVNAGIDWTNEQVKADITNFSHQVIIDEGESRM
CCCCCCHHHHHHHCCHHCCCEEEEEEEEEECCCCCCCCHHEEHHCCCCEEEEEECCCCCC
STGVYLGTEYQLEKLTLEANTRFDKHDKHDKYDEYVTWGVGASYQINEIYRISSAYNTAF
CCCEEECCCEEEEEEEEECCCCCCCCCCCCCCCCEEEECCCCCEEECCEEEEHHHHHHHC
KAPSFYDLSTAPDLEPETSDNIDVGLAAEYALVSVNLSLYKNKVDNLIIYYDAPNYMGYS
CCCCCEECCCCCCCCCCCCCCCEEEEEEEEEEEEEEHHHHHCCCCCEEEEECCCCCCCCC
ANVDADIKGAELELLFDTGIVSHTVIAEYKDHEDTQGQQLARRAKQNYKWISNVSFGDLD
CCCCCCCCCCEEEEEEECCCHHEEEEEECCCCCCCHHHHHHHHHHHCCEEECCCCCCCCC
MNLSYIYTGKRSDLPSAPGVATNYLDPYSLWTLAGAYWVTTELAVRGRVENLLDEEYETA
CCEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHCCEEEEEHHHHHHHHHHHHHHHHHHC
GGYKPAERSYYVSLDYQF
CCCCCCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA