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The map label for this gene is ghrA [H]

Identifier: 209696253

GI number: 209696253

Start: 3087597

End: 3088511

Strand: Direct

Name: ghrA [H]

Synonym: VSAL_I2849

Alternate gene names: 209696253

Gene position: 3087597-3088511 (Clockwise)

Preceding gene: 209696251

Following gene: 209696256

Centisome position: 92.86

GC content: 35.19

Gene sequence:

>915_bases
ATGAATAAATTACTTATCATTTCACAAGAAAAAGAGAAATATATCTCTCTTTTTGAGCAATCAGATCTTCCTGATCTTGA
AATTTGTCATGATCTGAAAAAAGCTGACATTATTCTTGCCGATCCACCATTGATTAAAGACCTTCTTAGTCAACTTAAAT
CAGTCAAATGGATCCAATCAACCTTCGCAGGGATTGATACGTTAATACAACCACATTTAAGACAAGACTACGATCTTACT
AATGTAAAAGGGATCTTCGGCCAACAAATTTCAGAGTATGTTTTAGGTTATTCTATTCAGTATTTCCAACACTTCCAACA
CTATGCTAAACAACAAAATAACAAAGAATGGTTAGTTCATTCTTATTCTAGTTTATCGGAAAAAAGCATGGTGATTTTAG
GTACGGGATCGATAGGTTGTTATTTAGCAAAGACAGCAAAAGCACTCGGTTTTAGTGTTTCTGGCATTAATCGCTCAGGT
ATCCCACCGAAAAACTCGCCATTTAATCAAGTATTTCATATCTCAGAAATAAAAAATGCGCTTAAAACGGCCGATGTCAT
TGTAAGTACTCTTCCTAAAACGGAACAAACAATAGACTTACTCAACATAGATACATTAGAAAATTGCAATCAGGCCATAT
TATTTAACGTTGGTCGTGGAGAAGTGTTGGATAACTATGGATTGTTGAATGCTCTCACTGCTGGATCGTTATCCCATGCT
TTTCTTGATGTGTTTAATAATGAGCCTTTATCACAGGAGTGCCCGTATTGGCACCACCCAAACATTACGATTACCCCTCA
TATCGCTGCACTTAGTTTTCCTGAGCAAGTATTTCAGCAATTCTCAGAAAACTATTTACGTTGGCATGATGGTTTTTCAT
TACTTAATCAAATTGATTTTGATAAAGGCTATTAA

Upstream 100 bases:

>100_bases
AAACGAACTTGGATAGGATTGCGTAACATATACGATTCTACACTATAAAAAAATGACGCTTTAAGTGTACTTGATAACCA
CGAATGACAAAAGGAAGGTT

Downstream 100 bases:

>100_bases
TCTCAAGCTTTCATTCTATTCACATATCGTTATGCGAATATTATTCACTCGCTTTTTTGGCTGCTAGTTTTGCTCGGATA
AATGCTGAATGTTCAATATA

Product: D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain

Products: NA

Alternate protein names: 2-ketoacid reductase [H]

Number of amino acids: Translated: 304; Mature: 304

Protein sequence:

>304_residues
MNKLLIISQEKEKYISLFEQSDLPDLEICHDLKKADIILADPPLIKDLLSQLKSVKWIQSTFAGIDTLIQPHLRQDYDLT
NVKGIFGQQISEYVLGYSIQYFQHFQHYAKQQNNKEWLVHSYSSLSEKSMVILGTGSIGCYLAKTAKALGFSVSGINRSG
IPPKNSPFNQVFHISEIKNALKTADVIVSTLPKTEQTIDLLNIDTLENCNQAILFNVGRGEVLDNYGLLNALTAGSLSHA
FLDVFNNEPLSQECPYWHHPNITITPHIAALSFPEQVFQQFSENYLRWHDGFSLLNQIDFDKGY

Sequences:

>Translated_304_residues
MNKLLIISQEKEKYISLFEQSDLPDLEICHDLKKADIILADPPLIKDLLSQLKSVKWIQSTFAGIDTLIQPHLRQDYDLT
NVKGIFGQQISEYVLGYSIQYFQHFQHYAKQQNNKEWLVHSYSSLSEKSMVILGTGSIGCYLAKTAKALGFSVSGINRSG
IPPKNSPFNQVFHISEIKNALKTADVIVSTLPKTEQTIDLLNIDTLENCNQAILFNVGRGEVLDNYGLLNALTAGSLSHA
FLDVFNNEPLSQECPYWHHPNITITPHIAALSFPEQVFQQFSENYLRWHDGFSLLNQIDFDKGY
>Mature_304_residues
MNKLLIISQEKEKYISLFEQSDLPDLEICHDLKKADIILADPPLIKDLLSQLKSVKWIQSTFAGIDTLIQPHLRQDYDLT
NVKGIFGQQISEYVLGYSIQYFQHFQHYAKQQNNKEWLVHSYSSLSEKSMVILGTGSIGCYLAKTAKALGFSVSGINRSG
IPPKNSPFNQVFHISEIKNALKTADVIVSTLPKTEQTIDLLNIDTLENCNQAILFNVGRGEVLDNYGLLNALTAGSLSHA
FLDVFNNEPLSQECPYWHHPNITITPHIAALSFPEQVFQQFSENYLRWHDGFSLLNQIDFDKGY

Specific function: Catalyzes the NADPH-dependent reduction of glyoxylate and hydroxypyruvate into glycolate and glycerate, respectively [H]

COG id: COG0111

COG function: function code HE; Phosphoglycerate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. GhrA subfamily [H]

Homologues:

Organism=Escherichia coli, GI87081824, Length=221, Percent_Identity=32.579185520362, Blast_Score=125, Evalue=4e-30,
Organism=Escherichia coli, GI87082289, Length=202, Percent_Identity=25.2475247524752, Blast_Score=69, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17532191, Length=257, Percent_Identity=22.9571984435798, Blast_Score=72, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6321253, Length=221, Percent_Identity=31.2217194570136, Blast_Score=80, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6325144, Length=158, Percent_Identity=32.2784810126582, Blast_Score=71, Evalue=2e-13,
Organism=Drosophila melanogaster, GI19921140, Length=298, Percent_Identity=25.1677852348993, Blast_Score=73, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.79; =1.1.1.81 [H]

Molecular weight: Translated: 34423; Mature: 34423

Theoretical pI: Translated: 5.94; Mature: 5.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKLLIISQEKEKYISLFEQSDLPDLEICHDLKKADIILADPPLIKDLLSQLKSVKWIQS
CCCEEEEECCHHHHHHHHHHCCCCHHHHHHHHHHCCEEEECCHHHHHHHHHHHHHHHHHH
TFAGIDTLIQPHLRQDYDLTNVKGIFGQQISEYVLGYSIQYFQHFQHYAKQQNNKEWLVH
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
SYSSLSEKSMVILGTGSIGCYLAKTAKALGFSVSGINRSGIPPKNSPFNQVFHISEIKNA
CHHCCCCCCEEEEECCCCHHHHHHHHHHHCCEECCCCCCCCCCCCCCHHHHEEHHHHHHH
LKTADVIVSTLPKTEQTIDLLNIDTLENCNQAILFNVGRGEVLDNYGLLNALTAGSLSHA
HHHHHHHHHHCCCCHHHEEEECCCCHHCCCCEEEEECCCCCCHHCCCHHHHHHHCCHHHH
FLDVFNNEPLSQECPYWHHPNITITPHIAALSFPEQVFQQFSENYLRWHDGFSLLNQIDF
HHHHHCCCCCCCCCCCCCCCCEEECCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
DKGY
CCCC
>Mature Secondary Structure
MNKLLIISQEKEKYISLFEQSDLPDLEICHDLKKADIILADPPLIKDLLSQLKSVKWIQS
CCCEEEEECCHHHHHHHHHHCCCCHHHHHHHHHHCCEEEECCHHHHHHHHHHHHHHHHHH
TFAGIDTLIQPHLRQDYDLTNVKGIFGQQISEYVLGYSIQYFQHFQHYAKQQNNKEWLVH
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
SYSSLSEKSMVILGTGSIGCYLAKTAKALGFSVSGINRSGIPPKNSPFNQVFHISEIKNA
CHHCCCCCCEEEEECCCCHHHHHHHHHHHCCEECCCCCCCCCCCCCCHHHHEEHHHHHHH
LKTADVIVSTLPKTEQTIDLLNIDTLENCNQAILFNVGRGEVLDNYGLLNALTAGSLSHA
HHHHHHHHHHCCCCHHHEEEECCCCHHCCCCEEEEECCCCCCHHCCCHHHHHHHCCHHHH
FLDVFNNEPLSQECPYWHHPNITITPHIAALSFPEQVFQQFSENYLRWHDGFSLLNQIDF
HHHHHCCCCCCCCCCCCCCCCEEECCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
DKGY
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA