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The map label for this gene is hcaT [H]

Identifier: 209696234

GI number: 209696234

Start: 3067539

End: 3068720

Strand: Reverse

Name: hcaT [H]

Synonym: VSAL_I2830

Alternate gene names: 209696234

Gene position: 3068720-3067539 (Counterclockwise)

Preceding gene: 209696244

Following gene: 209696233

Centisome position: 92.29

GC content: 43.49

Gene sequence:

>1182_bases
ATGACGGCCTCTTTTTTATTCCCTTTGGACATTGTCATGGTACGCGTTTCTCCCTTTGCTTGGATCTCTCAATATTTTGG
AGGGTTCTTTTTTGCCTATGGTGTGTATTTGCCTTTTTGGGCGCTGTGGTTTGCCGACCAAGGGGTATCAGCAGGAGATA
TTGGTGTTTTAATTGGTTTGGGATTTGCGACGCGTTGTGTGGCGAACTTAGTGATCACTCCTCGCTTACATAAAGTTGAA
TATTTAATTCCGGCCCTGCGTGCATTAACGTTTGCCTCCTTGATTGCACTTGGGTTGTTTCTTATTAATGGCGGCAACTT
CTGGTGGATGGCATTTGTCACCGTGTTGTTTAATTTGTCCTGTGGTCCGGCGATTCCTCTCTCCGATGCAATGACGAATT
ATTATGCGAAAAATAAATTATTAGATTATGGTCGAACTCGTTTATGGGGTTCAGTCAGCTTTATTGCCGGCTCAACGGTC
GTGGGTTACCTAGTGTCAGGCTTTGGCACCGACATGATTGTGTACACTGCGATTGCTGGTATTTTAGCGGCATTATTCTT
AGTGATGCGTAATCCAAATCCAATGCCAGTGTCAGTCTCGGAAGAAGACCAAACTCACCCTAAACTATTGGCCTTACTTC
GTGAAGCTCCGGTATTAAAATTTATTATTTTGGTGTCGTTAATTCAAGGTAGCCATGCCGCGTATTACAGTTTCAGCTCT
ATTTATTGGAAAGCGTCAGGCTATGAAGAAAGTACCATTGGTTATTTGTGGAGCTTAGGTGTCGTCGCTGAAGTGTTGGT
GTTTGCTTTTAGTAGACAATTGTTCGCGGGATGGAAAGTCTCGACTTTATTTAAAGTGGCAGCCGTTGGTGTCATGATCC
GCTGGGGATTAACGGCGGCAACGACAGAGTTATCGGCATTAATATTAGTTCAAGCACTGCATGGTGTGACCTTTGCGATG
GCACATTTGGCCGCCATTCAATACATTCAAAAATTCCCTCAAAACAAAATGATCCCACTACAAGCCTTATATAATGCGAT
TCCGCTTGGTGCCTTTATTGCCTTAATGACAACATTAAGTGGTTGGGGTTATGAAGTGTGGGGAGCGCATATTTTCTGGG
GTATGGCAGTGATGGGTGCCTTTGCCTTAATGATTAAGCTGGATAAAGTAAAAGATAAATAG

Upstream 100 bases:

>100_bases
TCTACCCATAAAGTTCTAAAATCTCGGGTTTATCACATTTTAAAAGGTGCATTTTTCTTCTGTTCTGTTTTCCCTTTTTA
TAAATCAATAGGATGCTATA

Downstream 100 bases:

>100_bases
GTCCTAATCACATTTGTTGGCTTGGATAAAAGCGAAGTAATAATAAAACGTTAGACTGGTGCGACAGAATATTCGTACCA
CTCTGGCGTTTTTTATGCTC

Product: 3-phenylpropionic acid transporter

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 393; Mature: 392

Protein sequence:

>393_residues
MTASFLFPLDIVMVRVSPFAWISQYFGGFFFAYGVYLPFWALWFADQGVSAGDIGVLIGLGFATRCVANLVITPRLHKVE
YLIPALRALTFASLIALGLFLINGGNFWWMAFVTVLFNLSCGPAIPLSDAMTNYYAKNKLLDYGRTRLWGSVSFIAGSTV
VGYLVSGFGTDMIVYTAIAGILAALFLVMRNPNPMPVSVSEEDQTHPKLLALLREAPVLKFIILVSLIQGSHAAYYSFSS
IYWKASGYEESTIGYLWSLGVVAEVLVFAFSRQLFAGWKVSTLFKVAAVGVMIRWGLTAATTELSALILVQALHGVTFAM
AHLAAIQYIQKFPQNKMIPLQALYNAIPLGAFIALMTTLSGWGYEVWGAHIFWGMAVMGAFALMIKLDKVKDK

Sequences:

>Translated_393_residues
MTASFLFPLDIVMVRVSPFAWISQYFGGFFFAYGVYLPFWALWFADQGVSAGDIGVLIGLGFATRCVANLVITPRLHKVE
YLIPALRALTFASLIALGLFLINGGNFWWMAFVTVLFNLSCGPAIPLSDAMTNYYAKNKLLDYGRTRLWGSVSFIAGSTV
VGYLVSGFGTDMIVYTAIAGILAALFLVMRNPNPMPVSVSEEDQTHPKLLALLREAPVLKFIILVSLIQGSHAAYYSFSS
IYWKASGYEESTIGYLWSLGVVAEVLVFAFSRQLFAGWKVSTLFKVAAVGVMIRWGLTAATTELSALILVQALHGVTFAM
AHLAAIQYIQKFPQNKMIPLQALYNAIPLGAFIALMTTLSGWGYEVWGAHIFWGMAVMGAFALMIKLDKVKDK
>Mature_392_residues
TASFLFPLDIVMVRVSPFAWISQYFGGFFFAYGVYLPFWALWFADQGVSAGDIGVLIGLGFATRCVANLVITPRLHKVEY
LIPALRALTFASLIALGLFLINGGNFWWMAFVTVLFNLSCGPAIPLSDAMTNYYAKNKLLDYGRTRLWGSVSFIAGSTVV
GYLVSGFGTDMIVYTAIAGILAALFLVMRNPNPMPVSVSEEDQTHPKLLALLREAPVLKFIILVSLIQGSHAAYYSFSSI
YWKASGYEESTIGYLWSLGVVAEVLVFAFSRQLFAGWKVSTLFKVAAVGVMIRWGLTAATTELSALILVQALHGVTFAMA
HLAAIQYIQKFPQNKMIPLQALYNAIPLGAFIALMTTLSGWGYEVWGAHIFWGMAVMGAFALMIKLDKVKDK

Specific function: Probable permease involved in the uptake of 3- phenylpropionic acid [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lacY/rafB permease family. HcaT subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788886, Length=368, Percent_Identity=39.1304347826087, Blast_Score=257, Evalue=8e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022814
- InterPro:   IPR016196
- InterPro:   IPR005276 [H]

Pfam domain/function: PF01306 LacY_symp [H]

EC number: NA

Molecular weight: Translated: 43186; Mature: 43054

Theoretical pI: Translated: 9.40; Mature: 9.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTASFLFPLDIVMVRVSPFAWISQYFGGFFFAYGVYLPFWALWFADQGVSAGDIGVLIGL
CCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHH
GFATRCVANLVITPRLHKVEYLIPALRALTFASLIALGLFLINGGNFWWMAFVTVLFNLS
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCC
CGPAIPLSDAMTNYYAKNKLLDYGRTRLWGSVSFIAGSTVVGYLVSGFGTDMIVYTAIAG
CCCCCCCHHHHHHHHHHHHHHHCCCHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
ILAALFLVMRNPNPMPVSVSEEDQTHPKLLALLREAPVLKFIILVSLIQGSHAAYYSFSS
HHHHHHHHHCCCCCCCEECCCCCCCHHHHHHHHHHCHHHHHHHHHHHHCCCCHHEEEHHH
IYWKASGYEESTIGYLWSLGVVAEVLVFAFSRQLFAGWKVSTLFKVAAVGVMIRWGLTAA
EEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHH
TTELSALILVQALHGVTFAMAHLAAIQYIQKFPQNKMIPLQALYNAIPLGAFIALMTTLS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHC
GWGYEVWGAHIFWGMAVMGAFALMIKLDKVKDK
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TASFLFPLDIVMVRVSPFAWISQYFGGFFFAYGVYLPFWALWFADQGVSAGDIGVLIGL
CCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHH
GFATRCVANLVITPRLHKVEYLIPALRALTFASLIALGLFLINGGNFWWMAFVTVLFNLS
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCC
CGPAIPLSDAMTNYYAKNKLLDYGRTRLWGSVSFIAGSTVVGYLVSGFGTDMIVYTAIAG
CCCCCCCHHHHHHHHHHHHHHHCCCHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
ILAALFLVMRNPNPMPVSVSEEDQTHPKLLALLREAPVLKFIILVSLIQGSHAAYYSFSS
HHHHHHHHHCCCCCCCEECCCCCCCHHHHHHHHHHCHHHHHHHHHHHHCCCCHHEEEHHH
IYWKASGYEESTIGYLWSLGVVAEVLVFAFSRQLFAGWKVSTLFKVAAVGVMIRWGLTAA
EEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHH
TTELSALILVQALHGVTFAMAHLAAIQYIQKFPQNKMIPLQALYNAIPLGAFIALMTTLS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHC
GWGYEVWGAHIFWGMAVMGAFALMIKLDKVKDK
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9603882; 9205837; 9278503 [H]