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The map label for this gene is aspA [H]

Identifier: 209696212

GI number: 209696212

Start: 3037403

End: 3038851

Strand: Direct

Name: aspA [H]

Synonym: VSAL_I2806

Alternate gene names: 209696212

Gene position: 3037403-3038851 (Clockwise)

Preceding gene: 209696210

Following gene: 209696213

Centisome position: 91.35

GC content: 40.92

Gene sequence:

>1449_bases
ATGACTCAGATGCTTGATTTAGAAAAAACAAATACCGCAACTCGTATTGAAGAAGATTTACTAGGTGAACGCCACGTTCC
CGCTGATGCTTACTACGGCATCCATACTCTTCGTGCGATGGAAAACTTCAACATTTCAAACGTCACGATCTCTGATGTTC
CAGAATTAGTTCGTGGCATGATCTATACGAAAAAAGCAGCTGCTTTAGCAAACAAAGAATTAGGTGCTATTCCTTCACAA
GTTGGTGAATACATCATCAAAGCATGTGACTTAATTCTTGAAACTGGCAAATGCATGGATCAGTTCCCATCAGATGTATA
TCAAGGTGGCGCTGGTACCTCTGTAAACATGAACGCAAATGAAGTTATCGCAAACGTTGCTCTTGAACTTATGGGCAAAG
AGAAAGGCGAATACGACATCATTAACCCTAACGATCACGTTAACCGCTCTCAATCAACTAACTGTGCATACCCAACGGGT
TTCCGCGTTGCTGTATACAACAGCATGATGAAATTGATTGAAGCGATCGAATACCTAAAAGGTGCATTCGATCTTAAAGA
TCAAGAATTCCGTAACGTTCTAAAAATGGGTCGTACTCAACTTCAAGATGCAGTTCCAATGACAGTAGGTCAAGAATTCC
ACGCTTGGGGCGTATTGCTTAAAGAAGAAGTGAAAAACCTTAATTACACAGCGCAACTTCTTTTAGAAGTAAACATCGGT
GCAACGGCAATCGGTACTGGCTTAAACGCGGCGACGGGTTACCAAGAAGCGGCAGTTCGTCATCTAGCTGAAATAACGGG
TCTAGAATGTGTTGCTGCTGAAGATTTAATCGAAGCGACTTCTGACTGTGGTGCTTACGTAATGATCCACGGTTCACTAA
AACGTACTGCGGTTAAATTATCTAAAATCTGTAACGATTTACGTCTTCTTTCTTCTGGCCCTCGTGCTGGTTTCAATGAA
TTGAACTTACCTGAGCTTCAAGCGGGTTCTTCAATCATGCCTGCAAAAGTAAACCCAGTAATTCCAGAAGTTGTAAACCA
AGTATGTTTCAAAGTAATTGGTAACGATACAACAGTGACTTTCGCTGCTGAAGCGGGCCAACTGCAATTAAACGTAATGG
AACCTGTTATTGGTCAAGCCCTATTCGAATCTATCGAAATCTTGAAAAATGCATGTGTAAACTTACGTGACAAGTGTATC
GATGGTATCACTGTAAACAAAGAAATATGTGAAGCTCATGTATTTAACTCTATCGGTATCGTTACTTACCTAAACCCATT
CATTGGCCACCACGAAGGTGACATTGTTGGTAAGATTTGTGCTGAAACAGGTAAAAACGTTCGCGATGTAGTTCTTGAAC
GTGGTCTTCTAACAGAAGAGCAACTAGATGATATCTTCTCAGTAGAAAACCTGATGCACCCACAATACAAAGCAAAACGT
TACGAATAA

Upstream 100 bases:

>100_bases
AGGTACGATATTGATCACTTCTGACGAACGGACTCTACTCGCAGGCATGGTCACTTGGAAAGAATCCAATAATTAAAATA
TAGAACCTTAAAGGCTTACT

Downstream 100 bases:

>100_bases
TTCGTATCACCTCAAATAGCGAGCTCACTGAAGCTCGCTTTTTTTATCTTCTCAATATTTTATTTACAAAACACTCAATT
AAACATTCATTCATCTTTTT

Product: aspartate ammonia-lyase

Products: NA

Alternate protein names: Aspartase [H]

Number of amino acids: Translated: 482; Mature: 481

Protein sequence:

>482_residues
MTQMLDLEKTNTATRIEEDLLGERHVPADAYYGIHTLRAMENFNISNVTISDVPELVRGMIYTKKAAALANKELGAIPSQ
VGEYIIKACDLILETGKCMDQFPSDVYQGGAGTSVNMNANEVIANVALELMGKEKGEYDIINPNDHVNRSQSTNCAYPTG
FRVAVYNSMMKLIEAIEYLKGAFDLKDQEFRNVLKMGRTQLQDAVPMTVGQEFHAWGVLLKEEVKNLNYTAQLLLEVNIG
ATAIGTGLNAATGYQEAAVRHLAEITGLECVAAEDLIEATSDCGAYVMIHGSLKRTAVKLSKICNDLRLLSSGPRAGFNE
LNLPELQAGSSIMPAKVNPVIPEVVNQVCFKVIGNDTTVTFAAEAGQLQLNVMEPVIGQALFESIEILKNACVNLRDKCI
DGITVNKEICEAHVFNSIGIVTYLNPFIGHHEGDIVGKICAETGKNVRDVVLERGLLTEEQLDDIFSVENLMHPQYKAKR
YE

Sequences:

>Translated_482_residues
MTQMLDLEKTNTATRIEEDLLGERHVPADAYYGIHTLRAMENFNISNVTISDVPELVRGMIYTKKAAALANKELGAIPSQ
VGEYIIKACDLILETGKCMDQFPSDVYQGGAGTSVNMNANEVIANVALELMGKEKGEYDIINPNDHVNRSQSTNCAYPTG
FRVAVYNSMMKLIEAIEYLKGAFDLKDQEFRNVLKMGRTQLQDAVPMTVGQEFHAWGVLLKEEVKNLNYTAQLLLEVNIG
ATAIGTGLNAATGYQEAAVRHLAEITGLECVAAEDLIEATSDCGAYVMIHGSLKRTAVKLSKICNDLRLLSSGPRAGFNE
LNLPELQAGSSIMPAKVNPVIPEVVNQVCFKVIGNDTTVTFAAEAGQLQLNVMEPVIGQALFESIEILKNACVNLRDKCI
DGITVNKEICEAHVFNSIGIVTYLNPFIGHHEGDIVGKICAETGKNVRDVVLERGLLTEEQLDDIFSVENLMHPQYKAKR
YE
>Mature_481_residues
TQMLDLEKTNTATRIEEDLLGERHVPADAYYGIHTLRAMENFNISNVTISDVPELVRGMIYTKKAAALANKELGAIPSQV
GEYIIKACDLILETGKCMDQFPSDVYQGGAGTSVNMNANEVIANVALELMGKEKGEYDIINPNDHVNRSQSTNCAYPTGF
RVAVYNSMMKLIEAIEYLKGAFDLKDQEFRNVLKMGRTQLQDAVPMTVGQEFHAWGVLLKEEVKNLNYTAQLLLEVNIGA
TAIGTGLNAATGYQEAAVRHLAEITGLECVAAEDLIEATSDCGAYVMIHGSLKRTAVKLSKICNDLRLLSSGPRAGFNEL
NLPELQAGSSIMPAKVNPVIPEVVNQVCFKVIGNDTTVTFAAEAGQLQLNVMEPVIGQALFESIEILKNACVNLRDKCID
GITVNKEICEAHVFNSIGIVTYLNPFIGHHEGDIVGKICAETGKNVRDVVLERGLLTEEQLDDIFSVENLMHPQYKAKRY
E

Specific function: Unknown

COG id: COG1027

COG function: function code E; Aspartate ammonia-lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II fumarase/aspartase family. Aspartase subfamily [H]

Homologues:

Organism=Homo sapiens, GI19743875, Length=462, Percent_Identity=38.7445887445887, Blast_Score=324, Evalue=1e-88,
Organism=Escherichia coli, GI87082375, Length=467, Percent_Identity=77.0877944325482, Blast_Score=738, Evalue=0.0,
Organism=Escherichia coli, GI1787896, Length=459, Percent_Identity=36.8191721132898, Blast_Score=306, Evalue=2e-84,
Organism=Escherichia coli, GI1790398, Length=319, Percent_Identity=25.3918495297806, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17553882, Length=462, Percent_Identity=39.1774891774892, Blast_Score=335, Evalue=4e-92,
Organism=Caenorhabditis elegans, GI32565146, Length=327, Percent_Identity=38.2262996941896, Blast_Score=238, Evalue=4e-63,
Organism=Saccharomyces cerevisiae, GI6324993, Length=472, Percent_Identity=38.135593220339, Blast_Score=331, Evalue=1e-91,
Organism=Drosophila melanogaster, GI24640177, Length=464, Percent_Identity=39.4396551724138, Blast_Score=333, Evalue=2e-91,
Organism=Drosophila melanogaster, GI24640179, Length=464, Percent_Identity=39.4396551724138, Blast_Score=332, Evalue=2e-91,
Organism=Drosophila melanogaster, GI24662684, Length=464, Percent_Identity=37.9310344827586, Blast_Score=327, Evalue=1e-89,
Organism=Drosophila melanogaster, GI78710009, Length=464, Percent_Identity=37.5, Blast_Score=324, Evalue=1e-88,
Organism=Drosophila melanogaster, GI24583245, Length=453, Percent_Identity=34.4370860927152, Blast_Score=275, Evalue=4e-74,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004708
- InterPro:   IPR003031
- InterPro:   IPR018951
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761 [H]

Pfam domain/function: PF10415 FumaraseC_C; PF00206 Lyase_1 [H]

EC number: =4.3.1.1 [H]

Molecular weight: Translated: 52835; Mature: 52704

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQMLDLEKTNTATRIEEDLLGERHVPADAYYGIHTLRAMENFNISNVTISDVPELVRGM
CCCCCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHH
IYTKKAAALANKELGAIPSQVGEYIIKACDLILETGKCMDQFPSDVYQGGAGTSVNMNAN
HHHHHHHHHHCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCEECCCHH
EVIANVALELMGKEKGEYDIINPNDHVNRSQSTNCAYPTGFRVAVYNSMMKLIEAIEYLK
HHHHHHHHHHHCCCCCCEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
GAFDLKDQEFRNVLKMGRTQLQDAVPMTVGQEFHAWGVLLKEEVKNLNYTAQLLLEVNIG
HHCCCCHHHHHHHHHHHHHHHHHCCCHHHCCHHHHHHHHHHHHHHCCCCEEEEEEEECCC
ATAIGTGLNAATGYQEAAVRHLAEITGLECVAAEDLIEATSDCGAYVMIHGSLKRTAVKL
CEECCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHH
SKICNDLRLLSSGPRAGFNELNLPELQAGSSIMPAKVNPVIPEVVNQVCFKVIGNDTTVT
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEE
FAAEAGQLQLNVMEPVIGQALFESIEILKNACVNLRDKCIDGITVNKEICEAHVFNSIGI
EEECCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCH
VTYLNPFIGHHEGDIVGKICAETGKNVRDVVLERGLLTEEQLDDIFSVENLMHPQYKAKR
HHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHCC
YE
CC
>Mature Secondary Structure 
TQMLDLEKTNTATRIEEDLLGERHVPADAYYGIHTLRAMENFNISNVTISDVPELVRGM
CCCCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHH
IYTKKAAALANKELGAIPSQVGEYIIKACDLILETGKCMDQFPSDVYQGGAGTSVNMNAN
HHHHHHHHHHCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCEECCCHH
EVIANVALELMGKEKGEYDIINPNDHVNRSQSTNCAYPTGFRVAVYNSMMKLIEAIEYLK
HHHHHHHHHHHCCCCCCEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
GAFDLKDQEFRNVLKMGRTQLQDAVPMTVGQEFHAWGVLLKEEVKNLNYTAQLLLEVNIG
HHCCCCHHHHHHHHHHHHHHHHHCCCHHHCCHHHHHHHHHHHHHHCCCCEEEEEEEECCC
ATAIGTGLNAATGYQEAAVRHLAEITGLECVAAEDLIEATSDCGAYVMIHGSLKRTAVKL
CEECCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHH
SKICNDLRLLSSGPRAGFNELNLPELQAGSSIMPAKVNPVIPEVVNQVCFKVIGNDTTVT
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEE
FAAEAGQLQLNVMEPVIGQALFESIEILKNACVNLRDKCIDGITVNKEICEAHVFNSIGI
EEECCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCH
VTYLNPFIGHHEGDIVGKICAETGKNVRDVVLERGLLTEEQLDDIFSVENLMHPQYKAKR
HHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHCC
YE
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]