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The map label for this gene is purA [H]

Identifier: 209696178

GI number: 209696178

Start: 3001857

End: 3003173

Strand: Reverse

Name: purA [H]

Synonym: VSAL_I2772

Alternate gene names: 209696178

Gene position: 3003173-3001857 (Counterclockwise)

Preceding gene: 209696179

Following gene: 209696177

Centisome position: 90.32

GC content: 42.37

Gene sequence:

>1317_bases
ATGGGAAATAACGTAGTCGTTCTGGGCACCCAATGGGGTGACGAAGGTAAAGGTAAAATAGTAGATCTTTTAACTGAAGA
TGCAAAGTATGTGGTTCGCTATCAAGGCGGTCACAATGCAGGCCATACTCTAGTTATCGACGGTGAGAAAACCGTTCTAC
ACTTAATTCCATCAGGTATTTTACGCAATAATGTTGAATGCATTATCGGTAATGGTGTAGTGCTTTCACCTGATGCCTTA
CTAAAAGAAATGGCGCCACTTGAAGCGCGTGGCATTCCAGTTCGTGAACGCCTATTCATTTCTGAAGCTTGTCCTCTAAT
TCTTCCTTACCACATCGCATTAGACCAAGCGCGTGAGCTTGCTCGTGGTAATAAAGCAATTGGCACAACTGGTCGCGGTA
TCGGTCCAGCGTACGAAGATAAAGTATCTCGTCGTGGTCTACGTGTTGGCGATCTGTTTGATAAAGTGACTTTTGCAGAA
AAACTAAAAGAAGTAATGGAATACCATAACTTCCAATTAGTAAATTACTATAAAGTAGAAGAAGTAAACTACGAAGAAGT
ACTTGAGCAAGTGATGAGCTATGCTGATCTACTTGTATCGATGGTTATTGATGTTACTGATACTCTTGATGCAGCGCGTA
AGCGTGGCGACAAAATCATGTTTGAAGGTGCTCAAGGTACGCTTCTAGATATCGACCACGGTACATACCCATATGTAACT
TCTTCGAACACAACCGCTGGTGGTGTTGCTGCTGGTTCTGGTTTTGGTCCTCGTCACTTAGGTTACATTCTAGGTATTAC
CAAAGCATATTGTACTCGTGTAGGTTCAGGTCCATTCCCTACAGAGCTATATGACGGTCTAGATAAACAAGACCCAATTG
GTAAGCACTTAGGCGATGTTGGTCATGAGTTTGGTGCAACAACAGGCCGTTTACGCCGTTGTGGTTGGTTCGATGCTGTT
GCTATGCGTCGTGCAATTCAAATCAACTCATTATCTGGAATTTGTCTAACTAAACTAGACGTTTTAGATGGCCTAAAAGA
GCTAAAAATCTGTACTGGTTACAAGATGAAAGATGGCTCTTTATTAGAAGTTTCTCCAATGGCTGCTGAAGCATTTGAAG
AAGCGACGCCAGTATATGAAACTATGCCTGGTTGGTCTGAGAATACATTCGGTGCACAAACAATCGAAGCGCTTCCACAA
GCGGCTCTAGATTACATTAAGCGTATCGAAGAACTAACAGGTGTTCCAATTGACATTATCTCTACTGGTCCTGACCGTAA
TGAGACAATCGTTAAAGTACACCCATTCGAAGTTTAA

Upstream 100 bases:

>100_bases
AGCGCTTTATTGTGAAAGTGAGATTTCGCTTAAAAATAACTGAAACATTTTTCTATCAATGCTAGAATCCATTTTTAACT
AACAAGCAGACTTTGGAAAG

Downstream 100 bases:

>100_bases
TAACGAACGAATGAATAAAAGCCAGCTCATGTAGTTGGTTTTTTTGTGCCTGAAATTTGAGAATAGATATAGAATTCGAT
CTAATCGGTAGCGTGATTTT

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase [H]

Number of amino acids: Translated: 438; Mature: 437

Protein sequence:

>438_residues
MGNNVVVLGTQWGDEGKGKIVDLLTEDAKYVVRYQGGHNAGHTLVIDGEKTVLHLIPSGILRNNVECIIGNGVVLSPDAL
LKEMAPLEARGIPVRERLFISEACPLILPYHIALDQARELARGNKAIGTTGRGIGPAYEDKVSRRGLRVGDLFDKVTFAE
KLKEVMEYHNFQLVNYYKVEEVNYEEVLEQVMSYADLLVSMVIDVTDTLDAARKRGDKIMFEGAQGTLLDIDHGTYPYVT
SSNTTAGGVAAGSGFGPRHLGYILGITKAYCTRVGSGPFPTELYDGLDKQDPIGKHLGDVGHEFGATTGRLRRCGWFDAV
AMRRAIQINSLSGICLTKLDVLDGLKELKICTGYKMKDGSLLEVSPMAAEAFEEATPVYETMPGWSENTFGAQTIEALPQ
AALDYIKRIEELTGVPIDIISTGPDRNETIVKVHPFEV

Sequences:

>Translated_438_residues
MGNNVVVLGTQWGDEGKGKIVDLLTEDAKYVVRYQGGHNAGHTLVIDGEKTVLHLIPSGILRNNVECIIGNGVVLSPDAL
LKEMAPLEARGIPVRERLFISEACPLILPYHIALDQARELARGNKAIGTTGRGIGPAYEDKVSRRGLRVGDLFDKVTFAE
KLKEVMEYHNFQLVNYYKVEEVNYEEVLEQVMSYADLLVSMVIDVTDTLDAARKRGDKIMFEGAQGTLLDIDHGTYPYVT
SSNTTAGGVAAGSGFGPRHLGYILGITKAYCTRVGSGPFPTELYDGLDKQDPIGKHLGDVGHEFGATTGRLRRCGWFDAV
AMRRAIQINSLSGICLTKLDVLDGLKELKICTGYKMKDGSLLEVSPMAAEAFEEATPVYETMPGWSENTFGAQTIEALPQ
AALDYIKRIEELTGVPIDIISTGPDRNETIVKVHPFEV
>Mature_437_residues
GNNVVVLGTQWGDEGKGKIVDLLTEDAKYVVRYQGGHNAGHTLVIDGEKTVLHLIPSGILRNNVECIIGNGVVLSPDALL
KEMAPLEARGIPVRERLFISEACPLILPYHIALDQARELARGNKAIGTTGRGIGPAYEDKVSRRGLRVGDLFDKVTFAEK
LKEVMEYHNFQLVNYYKVEEVNYEEVLEQVMSYADLLVSMVIDVTDTLDAARKRGDKIMFEGAQGTLLDIDHGTYPYVTS
SNTTAGGVAAGSGFGPRHLGYILGITKAYCTRVGSGPFPTELYDGLDKQDPIGKHLGDVGHEFGATTGRLRRCGWFDAVA
MRRAIQINSLSGICLTKLDVLDGLKELKICTGYKMKDGSLLEVSPMAAEAFEEATPVYETMPGWSENTFGAQTIEALPQA
ALDYIKRIEELTGVPIDIISTGPDRNETIVKVHPFEV

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP [H]

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family [H]

Homologues:

Organism=Homo sapiens, GI22748717, Length=431, Percent_Identity=42.6914153132251, Blast_Score=324, Evalue=1e-88,
Organism=Homo sapiens, GI34577063, Length=438, Percent_Identity=42.0091324200913, Blast_Score=315, Evalue=6e-86,
Organism=Homo sapiens, GI40316944, Length=402, Percent_Identity=40.547263681592, Blast_Score=281, Evalue=1e-75,
Organism=Escherichia coli, GI1790620, Length=437, Percent_Identity=75.7437070938215, Blast_Score=683, Evalue=0.0,
Organism=Caenorhabditis elegans, GI25146555, Length=437, Percent_Identity=43.7070938215103, Blast_Score=330, Evalue=1e-90,
Organism=Caenorhabditis elegans, GI25146553, Length=437, Percent_Identity=43.7070938215103, Blast_Score=329, Evalue=2e-90,
Organism=Saccharomyces cerevisiae, GI6324109, Length=438, Percent_Identity=41.0958904109589, Blast_Score=323, Evalue=3e-89,
Organism=Drosophila melanogaster, GI21356033, Length=431, Percent_Identity=41.5313225058005, Blast_Score=326, Evalue=2e-89,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018220
- InterPro:   IPR001114 [H]

Pfam domain/function: PF00709 Adenylsucc_synt [H]

EC number: =6.3.4.4 [H]

Molecular weight: Translated: 47849; Mature: 47718

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1 ; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGNNVVVLGTQWGDEGKGKIVDLLTEDAKYVVRYQGGHNAGHTLVIDGEKTVLHLIPSGI
CCCCEEEEEECCCCCCCCCEEEEECCCCEEEEEECCCCCCCCEEEECCCCEEEEECCHHH
LRNNVECIIGNGVVLSPDALLKEMAPLEARGIPVRERLFISEACPLILPYHIALDQAREL
HHCCCEEEEECCEEECHHHHHHHHCCCCCCCCCHHHHHHHHHCCCEEEHHHHHHHHHHHH
ARGNKAIGTTGRGIGPAYEDKVSRRGLRVGDLFDKVTFAEKLKEVMEYHNFQLVNYYKVE
HCCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEEC
EVNYEEVLEQVMSYADLLVSMVIDVTDTLDAARKRGDKIMFEGAQGTLLDIDHGTYPYVT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEECCCCCCCEEE
SSNTTAGGVAAGSGFGPRHLGYILGITKAYCTRVGSGPFPTELYDGLDKQDPIGKHLGDV
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCHHHHHHHH
GHEFGATTGRLRRCGWFDAVAMRRAIQINSLSGICLTKLDVLDGLKELKICTGYKMKDGS
HHHHCCCHHHHHHCCHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHCCCEECCCC
LLEVSPMAAEAFEEATPVYETMPGWSENTFGAQTIEALPQAALDYIKRIEELTGVPIDII
EEEECHHHHHHHHHCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
STGPDRNETIVKVHPFEV
ECCCCCCCEEEEEECCCC
>Mature Secondary Structure 
GNNVVVLGTQWGDEGKGKIVDLLTEDAKYVVRYQGGHNAGHTLVIDGEKTVLHLIPSGI
CCCEEEEEECCCCCCCCCEEEEECCCCEEEEEECCCCCCCCEEEECCCCEEEEECCHHH
LRNNVECIIGNGVVLSPDALLKEMAPLEARGIPVRERLFISEACPLILPYHIALDQAREL
HHCCCEEEEECCEEECHHHHHHHHCCCCCCCCCHHHHHHHHHCCCEEEHHHHHHHHHHHH
ARGNKAIGTTGRGIGPAYEDKVSRRGLRVGDLFDKVTFAEKLKEVMEYHNFQLVNYYKVE
HCCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEEC
EVNYEEVLEQVMSYADLLVSMVIDVTDTLDAARKRGDKIMFEGAQGTLLDIDHGTYPYVT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEECCCCCCCEEE
SSNTTAGGVAAGSGFGPRHLGYILGITKAYCTRVGSGPFPTELYDGLDKQDPIGKHLGDV
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCHHHHHHHH
GHEFGATTGRLRRCGWFDAVAMRRAIQINSLSGICLTKLDVLDGLKELKICTGYKMKDGS
HHHHCCCHHHHHHCCHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHCCCEECCCC
LLEVSPMAAEAFEEATPVYETMPGWSENTFGAQTIEALPQAALDYIKRIEELTGVPIDII
EEEECHHHHHHHHHCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
STGPDRNETIVKVHPFEV
ECCCCCCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA