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The map label for this gene is metF [H]

Identifier: 209696168

GI number: 209696168

Start: 2992910

End: 2993806

Strand: Reverse

Name: metF [H]

Synonym: VSAL_I2762

Alternate gene names: 209696168

Gene position: 2993806-2992910 (Counterclockwise)

Preceding gene: 209696169

Following gene: 209696167

Centisome position: 90.03

GC content: 40.13

Gene sequence:

>897_bases
ATGGCCTATTCACACGCAAGCCACTTAGAGTCGTTAAATCAAAATATTGCAGAGCTTAATGGCTGTATCAATGTATCTTT
TGAGTTTTTTCCACCAAGTTCAGAGAAGATGGAAGAAACGCTCTGGAACTCAATTCATCGTTTAAAAACACTAAAACCAA
AATTTGTGTCTGTAACGTATGGGGCTAACTCTGGTGAACGTGATCGTACCCATTCCATTATTAAAGAAATTAAAGAGCAA
ACGGGTCTTATTGCTGCTCCGCATTTAACGTGTATTGATGCGAGCCGTTCAGAGTTAATCGACATTGCGAATGATTACTG
GGCAAATGGTATTAAAGATATTGTTGCATTGCGTGGTGATATTCCAGCGGGTGGTGGTGCACCAGATATGTATGCTTCAG
ATTTGGTTGAGTTACTAAAATCACAACATGATTTTGATATTTCAGTGGCTGCATTTCCTGAAGTACATCCAGAAGCCAAA
AGCGCACAAGCGGATTTATTAAACCTAAAACGTAAAGTAGATGCGGGTGCTAACCGTGCGATTACTCAGTTTTTCTTTGA
TGTGGAAAGCTATTTACGTTTCCGTGATCGCTGTGTTTCTGCGGGCATTGATGTTGAAATTATTCCAGGTATTTTACCCG
TTTCAAATTTCAAGCAAGCCTCACGTTTTGCTAAAATGAATAACGTAAGAATCCCAGGTTGGATGGCTCAGCAATTTGAA
GGCTTGGATGATGATCCTGTGACTCGTCAAATGGTTGGGGCAAGTCACGCGATTGATATGACGCGAATTTTAAGTCGTGA
AGGCGTAAAAGATTTTCACTTCTATACGTTAAATCGTGCAGAACATACTTATGCTCTTTGTCATACATTAGGGGTTCGTC
CTGACGTTAATCGTTAA

Upstream 100 bases:

>100_bases
ATAACATACATTCTGTAGACATATAGACGTCCAAACATGATGTCACGGATGATAGAGTTTTGTAGTATTCCTTTTAATAC
TACAGCAGGGAGAGCAAGAC

Downstream 100 bases:

>100_bases
TGATAATGTATTAAGAAAAACCGCCATTTGTGGCGGTTTTTTTGTTTGTATAAGAGGATCTTTTAACGTAAGCGTGCGGG
GAACTACACCTAACAAATAA

Product: 5,10-methylenetetrahydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 298; Mature: 297

Protein sequence:

>298_residues
MAYSHASHLESLNQNIAELNGCINVSFEFFPPSSEKMEETLWNSIHRLKTLKPKFVSVTYGANSGERDRTHSIIKEIKEQ
TGLIAAPHLTCIDASRSELIDIANDYWANGIKDIVALRGDIPAGGGAPDMYASDLVELLKSQHDFDISVAAFPEVHPEAK
SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFKQASRFAKMNNVRIPGWMAQQFE
GLDDDPVTRQMVGASHAIDMTRILSREGVKDFHFYTLNRAEHTYALCHTLGVRPDVNR

Sequences:

>Translated_298_residues
MAYSHASHLESLNQNIAELNGCINVSFEFFPPSSEKMEETLWNSIHRLKTLKPKFVSVTYGANSGERDRTHSIIKEIKEQ
TGLIAAPHLTCIDASRSELIDIANDYWANGIKDIVALRGDIPAGGGAPDMYASDLVELLKSQHDFDISVAAFPEVHPEAK
SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFKQASRFAKMNNVRIPGWMAQQFE
GLDDDPVTRQMVGASHAIDMTRILSREGVKDFHFYTLNRAEHTYALCHTLGVRPDVNR
>Mature_297_residues
AYSHASHLESLNQNIAELNGCINVSFEFFPPSSEKMEETLWNSIHRLKTLKPKFVSVTYGANSGERDRTHSIIKEIKEQT
GLIAAPHLTCIDASRSELIDIANDYWANGIKDIVALRGDIPAGGGAPDMYASDLVELLKSQHDFDISVAAFPEVHPEAKS
AQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFKQASRFAKMNNVRIPGWMAQQFEG
LDDDPVTRQMVGASHAIDMTRILSREGVKDFHFYTLNRAEHTYALCHTLGVRPDVNR

Specific function: Methionine biosynthesis. [C]

COG id: COG0685

COG function: function code E; 5,10-methylenetetrahydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methylenetetrahydrofolate reductase family [H]

Homologues:

Organism=Homo sapiens, GI87240000, Length=281, Percent_Identity=33.0960854092527, Blast_Score=139, Evalue=3e-33,
Organism=Escherichia coli, GI1790377, Length=294, Percent_Identity=78.9115646258503, Blast_Score=489, Evalue=1e-140,
Organism=Caenorhabditis elegans, GI25147436, Length=278, Percent_Identity=31.6546762589928, Blast_Score=145, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI25147441, Length=278, Percent_Identity=31.6546762589928, Blast_Score=144, Evalue=4e-35,
Organism=Saccharomyces cerevisiae, GI6325234, Length=279, Percent_Identity=33.3333333333333, Blast_Score=143, Evalue=3e-35,
Organism=Saccharomyces cerevisiae, GI6321313, Length=300, Percent_Identity=29.3333333333333, Blast_Score=131, Evalue=1e-31,
Organism=Drosophila melanogaster, GI24662658, Length=225, Percent_Identity=24.4444444444444, Blast_Score=65, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR004620 [H]

Pfam domain/function: PF02219 MTHFR [H]

EC number: =1.5.1.20 [H]

Molecular weight: Translated: 33277; Mature: 33146

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAYSHASHLESLNQNIAELNGCINVSFEFFPPSSEKMEETLWNSIHRLKTLKPKFVSVTY
CCCCHHHHHHHHHHHHHHHCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCEEEEEE
GANSGERDRTHSIIKEIKEQTGLIAAPHLTCIDASRSELIDIANDYWANGIKDIVALRGD
CCCCCCCHHHHHHHHHHHHHCCCEECCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCC
IPAGGGAPDMYASDLVELLKSQHDFDISVAAFPEVHPEAKSAQADLLNLKRKVDAGANRA
CCCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCHH
ITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFKQASRFAKMNNVRIPGWMAQQFE
HHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHC
GLDDDPVTRQMVGASHAIDMTRILSREGVKDFHFYTLNRAEHTYALCHTLGVRPDVNR
CCCCCHHHHHHHCCHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
AYSHASHLESLNQNIAELNGCINVSFEFFPPSSEKMEETLWNSIHRLKTLKPKFVSVTY
CCCHHHHHHHHHHHHHHHCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCEEEEEE
GANSGERDRTHSIIKEIKEQTGLIAAPHLTCIDASRSELIDIANDYWANGIKDIVALRGD
CCCCCCCHHHHHHHHHHHHHCCCEECCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCC
IPAGGGAPDMYASDLVELLKSQHDFDISVAAFPEVHPEAKSAQADLLNLKRKVDAGANRA
CCCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCHH
ITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFKQASRFAKMNNVRIPGWMAQQFE
HHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHC
GLDDDPVTRQMVGASHAIDMTRILSREGVKDFHFYTLNRAEHTYALCHTLGVRPDVNR
CCCCCHHHHHHHCCHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA