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The map label for this gene is mrcA [H]

Identifier: 209696122

GI number: 209696122

Start: 2940107

End: 2942605

Strand: Reverse

Name: mrcA [H]

Synonym: VSAL_I2715

Alternate gene names: 209696122

Gene position: 2942605-2940107 (Counterclockwise)

Preceding gene: 209696127

Following gene: 209696120

Centisome position: 88.5

GC content: 41.66

Gene sequence:

>2499_bases
GTGAAGTTCATAAAGGCACTGCTCATAGTTACATTTTTTTGCATAATTATTGGGGTCACTACAATTTTTGGTTTTTACCA
ATATGTGAAACCAGAATTACCAGATGTCGCGACATTGAAAGACGTTCAGCTACAAACGCCAATGCAAGTTTATAGCCGCG
ATGGAAAGTTGATCGCTCAATTTGGTGAAAAACGCCGTATTCCTTTAAAATTGGAAGACATGCCCCCTCATTTACTTGAG
GCCATCATTGCAACTGAAGACAGTCGTTTCTATAGCCATTATGGTTTTGATCCTATTGGTATTACTCGTGCCGCATTTGC
AGTACTTGCTTCAGGTTCCGCAAAACAAGGGGCGAGTACCATTACTCAGCAACTTGCGCGTAATTTCTTCTTATCAAATG
AGAAGAAAATCATGCGAAAAATCAAAGAGATTTTTATCGCGGTTCACATTGAGCAATTGCTAACAAAACAAGAAATTTTA
GAACTCTATTTAAATAAAATTTACCTTGGTTACCGTTCATACGGTGTTGGTGCTGCCGCTCATGTTTATTTTGGTAAAGA
AGTAAACGAATTAACGCTAGGTGAAGTCGCTATCATTGCTGGCTTACCAAAAGCGCCTTCAACGATGAACCCTATTTATT
CAGTTGACCGTGCTACGACTCGTCGAAATGTCGTATTGGCTCGTATGCTCGATGAAGGCTATATCACAAAACAAGAATTT
GAAGACGCAAAAGCGGAAGTGGTTATTTCTAAATATCACGGCGCAGAAATTAAACTACAAGCCCCTTATGTTGCAGAAAT
AGCTCGTGCATGGATGGTGAATAAGTACGGTGAAGAAGCGGCTTATACATCAGGTATGAACATCTACACGACTGTTGATT
CAAAAATGCAAGCCGCTGCAAATAAAGCATCCATCGATAACTTACTTGCTTATGACGAACGCCATGGTTTCCGTGGTGCA
GTCAAAACAGTCTGGGAACCGAAAGCCACACCGCTTAATGAAGACGCGATGGCAAAACACTTGAAAAAAGAACCTTCGTA
CGGTGAGTTAATGCCTGCAATTGTGACTCAAGTGAAAGGAAAAACAGTCACGGTTAATATCAAAAATAACGGTACAGCAA
CCATTCCCTGGGATGGATTAAAATGGGCTCGTCGTTTCAAAACAGATAAACGCCAAGGTTCTGCACCTCGTCGTGCCTCT
GATATTTTGAGTGTTGGTGAGAAAATTTACGTTCGCCCTCTCAGCGAAGAAACAAAAGACGACGTTACGACTATCGTCTG
GAAACTAAGCCAAGTTCCTGCAGCGAATACCGCATTTGTTGCAATGGATCCTAATGACGGAGCGATTACCTCTCTGGTTG
GTGGTTTCAACTTTGTTCATAATAAATTTAACCGTGCGACTCAATCTGTTCGTCAAGTTGGTTCGAGTATTAAACCATTT
ATTTATTCCGCGGCGTTGTATCACGGTAAAACACTCGCAAGCTTAATTACCGATGCACCGATCAATACATGGGATGAAAG
CCAAGGTACGGCGTGGAGACCTAAAAACTCACCACCAACCTACATTGGTCCTGCTCGTTTACGTATTGGTTTAGCTCAAT
CGAAAAATGTAATGGCTGTTCGCGTTTTGCGTGATGTCGGCTTAGATGAAACGATTGATTATCTTACTCGTTTTGGTTTC
AAGAAAGATGAGTTACCTCGCTCAGATACCATTGCTCTTGGTGCAGGCAGTTTAAGCCCAGTGCAAATGGTACAAGGCTT
TTCAGTCTTTGCTAATAATGGTTACTATGTTGAACCTTATTATATAGACCATATTGAAAGCCCATACGGCGATGAGGTTT
ACAAAGCAACGCCAAAACTTATCTGCCAAAAAGAGTGCGATCACCAAAATGATGAAAACTCACCTTATGCGAAAAAAGTT
ATCTCAGCTCAAAATGCGTTCCTCACAAAACAGATGATGTATAGCAATATCTGGGGTGGTGGCGTATGGAGCAAAGGTAC
CGGTTGGAATGGTACAGGCTGGCGAGCACAAGTGCTGAAACGTCGTGATATTGGTGGTAAAACCGGTACAACCAATGACT
CTGTTGATGCATGGTATAACGGTTACGGTCCAAACGTTGTTGCAACGGCTTGGGTTGGTTTTGATAACCCATCACACCAT
TTAGGCAGCACAAGTAAAAATGACAACATGCCTAAAGATGAGATGCTAACTGGCGGAGAAGCTGGCGGTAAAACAGCAAT
CTACGCTTGGGTTAATTTCATGAAGGTTGCTTTAGAAGGTGTGCCTGAAGAAAACTTAAGACTTCCAGCCAACATCATTA
AAGTAAAAATAGACCGTGAAACCGGCCTATTAAGTAATAAAAATGATGAAACGAGTATGTGGGAATATTTTGCTGGAGGT
ACAGAGCCAACTGAATACGTAAAACAAGATTTCCAAGATACTATCTATTCATCAGGTGATGCTGATGGCGACGGCGAAGA
AGACGAAAGCTTATTCTAA

Upstream 100 bases:

>100_bases
TTAAGCGTTAATTTACGTTATTTTTCGTTGTGTTATATTTATACTACTCAGTTATTGGTTAGAAACGACAATACAGATTA
AAGTAAACGGGAAATATCAG

Downstream 100 bases:

>100_bases
GTACTTAGTTTAAAGTAAAGAACAAGGGACGCTCACTTAGCGTCCCTTTTTTTATTCGTATTTTATCTATAAAACAGTTT
GATGGATAACGTCAGCCATT

Product: penicillin-binding protein 1A

Products: NA

Alternate protein names: PBP-1a; PBP1a; Penicillin-insensitive transglycosylase; Peptidoglycan TGase; Penicillin-sensitive transpeptidase; DD-transpeptidase [H]

Number of amino acids: Translated: 832; Mature: 832

Protein sequence:

>832_residues
MKFIKALLIVTFFCIIIGVTTIFGFYQYVKPELPDVATLKDVQLQTPMQVYSRDGKLIAQFGEKRRIPLKLEDMPPHLLE
AIIATEDSRFYSHYGFDPIGITRAAFAVLASGSAKQGASTITQQLARNFFLSNEKKIMRKIKEIFIAVHIEQLLTKQEIL
ELYLNKIYLGYRSYGVGAAAHVYFGKEVNELTLGEVAIIAGLPKAPSTMNPIYSVDRATTRRNVVLARMLDEGYITKQEF
EDAKAEVVISKYHGAEIKLQAPYVAEIARAWMVNKYGEEAAYTSGMNIYTTVDSKMQAAANKASIDNLLAYDERHGFRGA
VKTVWEPKATPLNEDAMAKHLKKEPSYGELMPAIVTQVKGKTVTVNIKNNGTATIPWDGLKWARRFKTDKRQGSAPRRAS
DILSVGEKIYVRPLSEETKDDVTTIVWKLSQVPAANTAFVAMDPNDGAITSLVGGFNFVHNKFNRATQSVRQVGSSIKPF
IYSAALYHGKTLASLITDAPINTWDESQGTAWRPKNSPPTYIGPARLRIGLAQSKNVMAVRVLRDVGLDETIDYLTRFGF
KKDELPRSDTIALGAGSLSPVQMVQGFSVFANNGYYVEPYYIDHIESPYGDEVYKATPKLICQKECDHQNDENSPYAKKV
ISAQNAFLTKQMMYSNIWGGGVWSKGTGWNGTGWRAQVLKRRDIGGKTGTTNDSVDAWYNGYGPNVVATAWVGFDNPSHH
LGSTSKNDNMPKDEMLTGGEAGGKTAIYAWVNFMKVALEGVPEENLRLPANIIKVKIDRETGLLSNKNDETSMWEYFAGG
TEPTEYVKQDFQDTIYSSGDADGDGEEDESLF

Sequences:

>Translated_832_residues
MKFIKALLIVTFFCIIIGVTTIFGFYQYVKPELPDVATLKDVQLQTPMQVYSRDGKLIAQFGEKRRIPLKLEDMPPHLLE
AIIATEDSRFYSHYGFDPIGITRAAFAVLASGSAKQGASTITQQLARNFFLSNEKKIMRKIKEIFIAVHIEQLLTKQEIL
ELYLNKIYLGYRSYGVGAAAHVYFGKEVNELTLGEVAIIAGLPKAPSTMNPIYSVDRATTRRNVVLARMLDEGYITKQEF
EDAKAEVVISKYHGAEIKLQAPYVAEIARAWMVNKYGEEAAYTSGMNIYTTVDSKMQAAANKASIDNLLAYDERHGFRGA
VKTVWEPKATPLNEDAMAKHLKKEPSYGELMPAIVTQVKGKTVTVNIKNNGTATIPWDGLKWARRFKTDKRQGSAPRRAS
DILSVGEKIYVRPLSEETKDDVTTIVWKLSQVPAANTAFVAMDPNDGAITSLVGGFNFVHNKFNRATQSVRQVGSSIKPF
IYSAALYHGKTLASLITDAPINTWDESQGTAWRPKNSPPTYIGPARLRIGLAQSKNVMAVRVLRDVGLDETIDYLTRFGF
KKDELPRSDTIALGAGSLSPVQMVQGFSVFANNGYYVEPYYIDHIESPYGDEVYKATPKLICQKECDHQNDENSPYAKKV
ISAQNAFLTKQMMYSNIWGGGVWSKGTGWNGTGWRAQVLKRRDIGGKTGTTNDSVDAWYNGYGPNVVATAWVGFDNPSHH
LGSTSKNDNMPKDEMLTGGEAGGKTAIYAWVNFMKVALEGVPEENLRLPANIIKVKIDRETGLLSNKNDETSMWEYFAGG
TEPTEYVKQDFQDTIYSSGDADGDGEEDESLF
>Mature_832_residues
MKFIKALLIVTFFCIIIGVTTIFGFYQYVKPELPDVATLKDVQLQTPMQVYSRDGKLIAQFGEKRRIPLKLEDMPPHLLE
AIIATEDSRFYSHYGFDPIGITRAAFAVLASGSAKQGASTITQQLARNFFLSNEKKIMRKIKEIFIAVHIEQLLTKQEIL
ELYLNKIYLGYRSYGVGAAAHVYFGKEVNELTLGEVAIIAGLPKAPSTMNPIYSVDRATTRRNVVLARMLDEGYITKQEF
EDAKAEVVISKYHGAEIKLQAPYVAEIARAWMVNKYGEEAAYTSGMNIYTTVDSKMQAAANKASIDNLLAYDERHGFRGA
VKTVWEPKATPLNEDAMAKHLKKEPSYGELMPAIVTQVKGKTVTVNIKNNGTATIPWDGLKWARRFKTDKRQGSAPRRAS
DILSVGEKIYVRPLSEETKDDVTTIVWKLSQVPAANTAFVAMDPNDGAITSLVGGFNFVHNKFNRATQSVRQVGSSIKPF
IYSAALYHGKTLASLITDAPINTWDESQGTAWRPKNSPPTYIGPARLRIGLAQSKNVMAVRVLRDVGLDETIDYLTRFGF
KKDELPRSDTIALGAGSLSPVQMVQGFSVFANNGYYVEPYYIDHIESPYGDEVYKATPKLICQKECDHQNDENSPYAKKV
ISAQNAFLTKQMMYSNIWGGGVWSKGTGWNGTGWRAQVLKRRDIGGKTGTTNDSVDAWYNGYGPNVVATAWVGFDNPSHH
LGSTSKNDNMPKDEMLTGGEAGGKTAIYAWVNFMKVALEGVPEENLRLPANIIKVKIDRETGLLSNKNDETSMWEYFAGG
TEPTEYVKQDFQDTIYSSGDADGDGEEDESLF

Specific function: Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal

COG id: COG5009

COG function: function code M; Membrane carboxypeptidase/penicillin-binding protein

Gene ontology:

Cell location: Cell inner membrane; Single-pass type II membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI87082258, Length=867, Percent_Identity=49.3656286043829, Blast_Score=864, Evalue=0.0,
Organism=Escherichia coli, GI1786343, Length=287, Percent_Identity=32.404181184669, Blast_Score=139, Evalue=7e-34,
Organism=Escherichia coli, GI1788867, Length=251, Percent_Identity=33.4661354581673, Blast_Score=135, Evalue=1e-32,
Organism=Escherichia coli, GI1789601, Length=165, Percent_Identity=32.7272727272727, Blast_Score=84, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.2.-; 3.4.-.- [C]

Molecular weight: Translated: 92428; Mature: 92428

Theoretical pI: Translated: 8.03; Mature: 8.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFIKALLIVTFFCIIIGVTTIFGFYQYVKPELPDVATLKDVQLQTPMQVYSRDGKLIAQ
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHCCCHHHHH
FGEKRRIPLKLEDMPPHLLEAIIATEDSRFYSHYGFDPIGITRAAFAVLASGSAKQGAST
HCCCCCCCEEECCCCHHHHHHHHHCCCCHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHH
ITQQLARNFFLSNEKKIMRKIKEIFIAVHIEQLLTKQEILELYLNKIYLGYRSYGVGAAA
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEE
HVYFGKEVNELTLGEVAIIAGLPKAPSTMNPIYSVDRATTRRNVVLARMLDEGYITKQEF
EEEECCCCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
EDAKAEVVISKYHGAEIKLQAPYVAEIARAWMVNKYGEEAAYTSGMNIYTTVDSKMQAAA
HCCHHEEEEEEECCCEEEECCCHHHHHHHHHHHHHCCCHHHHHCCCEEEEECCHHHHHHH
NKASIDNLLAYDERHGFRGAVKTVWEPKATPLNEDAMAKHLKKEPSYGELMPAIVTQVKG
HHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCC
KTVTVNIKNNGTATIPWDGLKWARRFKTDKRQGSAPRRASDILSVGEKIYVRPLSEETKD
CEEEEEEECCCEEEECCCHHHHHHHHCCHHHCCCCCHHHHHHHHCCCEEEEEECCCCHHH
DVTTIVWKLSQVPAANTAFVAMDPNDGAITSLVGGFNFVHNKFNRATQSVRQVGSSIKPF
HHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
IYSAALYHGKTLASLITDAPINTWDESQGTAWRPKNSPPTYIGPARLRIGLAQSKNVMAV
HHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEECHHHEEEEECCCCCHHHH
RVLRDVGLDETIDYLTRFGFKKDELPRSDTIALGAGSLSPVQMVQGFSVFANNGYYVEPY
HHHHHCCCHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCHHHHHCCCEEEECCCEEECCE
YIDHIESPYGDEVYKATPKLICQKECDHQNDENSPYAKKVISAQNAFLTKQMMYSNIWGG
EECCCCCCCCCHHHHHCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
GVWSKGTGWNGTGWRAQVLKRRDIGGKTGTTNDSVDAWYNGYGPNVVATAWVGFDNPSHH
CCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHCCCCCCCEEEEEEECCCCCCCC
LGSTSKNDNMPKDEMLTGGEAGGKTAIYAWVNFMKVALEGVPEENLRLPANIIKVKIDRE
CCCCCCCCCCCHHHHCCCCCCCCCEEHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEECC
TGLLSNKNDETSMWEYFAGGTEPTEYVKQDFQDTIYSSGDADGDGEEDESLF
CCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKFIKALLIVTFFCIIIGVTTIFGFYQYVKPELPDVATLKDVQLQTPMQVYSRDGKLIAQ
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHCCCHHHHH
FGEKRRIPLKLEDMPPHLLEAIIATEDSRFYSHYGFDPIGITRAAFAVLASGSAKQGAST
HCCCCCCCEEECCCCHHHHHHHHHCCCCHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHH
ITQQLARNFFLSNEKKIMRKIKEIFIAVHIEQLLTKQEILELYLNKIYLGYRSYGVGAAA
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEE
HVYFGKEVNELTLGEVAIIAGLPKAPSTMNPIYSVDRATTRRNVVLARMLDEGYITKQEF
EEEECCCCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
EDAKAEVVISKYHGAEIKLQAPYVAEIARAWMVNKYGEEAAYTSGMNIYTTVDSKMQAAA
HCCHHEEEEEEECCCEEEECCCHHHHHHHHHHHHHCCCHHHHHCCCEEEEECCHHHHHHH
NKASIDNLLAYDERHGFRGAVKTVWEPKATPLNEDAMAKHLKKEPSYGELMPAIVTQVKG
HHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCC
KTVTVNIKNNGTATIPWDGLKWARRFKTDKRQGSAPRRASDILSVGEKIYVRPLSEETKD
CEEEEEEECCCEEEECCCHHHHHHHHCCHHHCCCCCHHHHHHHHCCCEEEEEECCCCHHH
DVTTIVWKLSQVPAANTAFVAMDPNDGAITSLVGGFNFVHNKFNRATQSVRQVGSSIKPF
HHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
IYSAALYHGKTLASLITDAPINTWDESQGTAWRPKNSPPTYIGPARLRIGLAQSKNVMAV
HHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEECHHHEEEEECCCCCHHHH
RVLRDVGLDETIDYLTRFGFKKDELPRSDTIALGAGSLSPVQMVQGFSVFANNGYYVEPY
HHHHHCCCHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCHHHHHCCCEEEECCCEEECCE
YIDHIESPYGDEVYKATPKLICQKECDHQNDENSPYAKKVISAQNAFLTKQMMYSNIWGG
EECCCCCCCCCHHHHHCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
GVWSKGTGWNGTGWRAQVLKRRDIGGKTGTTNDSVDAWYNGYGPNVVATAWVGFDNPSHH
CCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHCCCCCCCEEEEEEECCCCCCCC
LGSTSKNDNMPKDEMLTGGEAGGKTAIYAWVNFMKVALEGVPEENLRLPANIIKVKIDRE
CCCCCCCCCCCHHHHCCCCCCCCCEEHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEECC
TGLLSNKNDETSMWEYFAGGTEPTEYVKQDFQDTIYSSGDADGDGEEDESLF
CCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]