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The map label for this gene is lpdA [H]

Identifier: 209696119

GI number: 209696119

Start: 2936668

End: 2938134

Strand: Reverse

Name: lpdA [H]

Synonym: VSAL_I2712

Alternate gene names: 209696119

Gene position: 2938134-2936668 (Counterclockwise)

Preceding gene: 209696120

Following gene: 209696118

Centisome position: 88.36

GC content: 43.56

Gene sequence:

>1467_bases
ATGAAACAAGTCAATGTTGATGTAGCTGTTATCGGTGGTGGTACAGCAGGTTTAGGCTCTTACCGTGCAGCAAAAGCACA
TACTGACAGTGTTGTCATGATCGAAGGCGGCCCTTACGGTACCACTTGTGCTCGTGTTGGTTGCATGCCATCAAAACTGT
TAATTGCAGCGGCAGAAAGCGTTCACCAAATTGAAAAAGCACCTCAGTTCGGAATTCACCCTCAAGGTGACATCGTAATT
AACGGCCGCGAAGTCATGGCCCGTGTTAAATTTGAACGTGACCGTTTTGTTGGGTTTGTTTTAGAAGGCGTTGATGAAAT
CCCAGCGGAAGATAAAATCTCTGGCTACGCTAAATTTTTAAACGACAATACATTGCAAGTCGATGATCATACGATCATTA
CAGCAAAACGTATTGTTATTGCAACAGGCTCTCGCCCTGCTTACCCTGCGGTATGGAATGAATTAGGTGATCGCCTTATC
ATCAATGATGACGTATTTGATTGGGATGATTTACCGACATCAGTCGCCGTATTTGGTCCTGGCGTTATTGGTCTTGAACT
TGGACAATCACTTCACCGTTTAGGCGTTGAAACTAAGCTTTTTGGTTTAGGTGGTCAAGTCGGTCCGGTAACAGATCCTG
AAGTAATGGCTTATGCAAACAAGGCCTTCAACGAAGAGTTTTACCTTGATGCCGATGTGAAAATAGAGAGCATGAAACGC
ATATCTCTTAATGATGATGAAGATAAAGTTGAAATCAAATTCATCAATAAACAAGGCGAACAAGAAACGATCCTAGTTGA
GTATGTACTTGCAGCAACCGGTCGTCGTCCAAACACCGACAAATTGGGCTTAGAAAACACATTGGTTGAATTAGACGAAC
GCGGTGTACCAACAGCCGATCACTACACATTACAAACATCATTACCAACCATCTTCATTGCTGGTGATGCCAGTAATCAA
CTGCCCCTATTGCACGAGGCCGCTGATCAAGCACGTATTGCTGGTGATAATGCAGGACGTTTCCCAGAGATCCGCGCAGG
CCTTCGTCGCTCGAAAATCTCAGCGGTATTCTCAGATCCACAAATCGCAATGGTTGGAGAAAGCTATAAAGAAATCACAA
CTCGTTTAGGGACTTGTGGTTGTTTCGCAACTGGTGAGGTTTCTTTTGAAAATCAAGGCCGTTCACGAGTAATGCTGCGG
AACAAAGGAATTCTTCATGTATACGGTGAACAAGGAACGGGGCGTTTCTTAGGCGCTGAAATGATGGGACCAAATGCAGA
GCATTTAGCGCACCTATTAGCTTGGGCACACCAAAATAAAATGACTATTTCTGAAATGCTAGATATGCCATTTTATCACC
CAGTAATCGAAGAAGGTATTCGTACTGCACTTCGTGATTTAAATGCGAAACTGCATTTAGGCCCTGAAATGATTAAACAT
TGTATGGATTGTGGTCCTGGTTGTTAA

Upstream 100 bases:

>100_bases
TCCTAGCTTAATCTCTCGCTCAAAATGAATAAAAATGGCCCACGACACCGTGGGCCATTTCAAAACTTCTTCTAAAATGA
ATCTACGTGAGATATGAATT

Downstream 100 bases:

>100_bases
TCACCAGCAACTAACATAAAAACAAAATAAAAAAGGTCGATGTGAAAACATCGACCTTTTTTATTTCTATCTTACCCATC
ACTCGTTAAAATAACGGCCA

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 488; Mature: 488

Protein sequence:

>488_residues
MKQVNVDVAVIGGGTAGLGSYRAAKAHTDSVVMIEGGPYGTTCARVGCMPSKLLIAAAESVHQIEKAPQFGIHPQGDIVI
NGREVMARVKFERDRFVGFVLEGVDEIPAEDKISGYAKFLNDNTLQVDDHTIITAKRIVIATGSRPAYPAVWNELGDRLI
INDDVFDWDDLPTSVAVFGPGVIGLELGQSLHRLGVETKLFGLGGQVGPVTDPEVMAYANKAFNEEFYLDADVKIESMKR
ISLNDDEDKVEIKFINKQGEQETILVEYVLAATGRRPNTDKLGLENTLVELDERGVPTADHYTLQTSLPTIFIAGDASNQ
LPLLHEAADQARIAGDNAGRFPEIRAGLRRSKISAVFSDPQIAMVGESYKEITTRLGTCGCFATGEVSFENQGRSRVMLR
NKGILHVYGEQGTGRFLGAEMMGPNAEHLAHLLAWAHQNKMTISEMLDMPFYHPVIEEGIRTALRDLNAKLHLGPEMIKH
CMDCGPGC

Sequences:

>Translated_488_residues
MKQVNVDVAVIGGGTAGLGSYRAAKAHTDSVVMIEGGPYGTTCARVGCMPSKLLIAAAESVHQIEKAPQFGIHPQGDIVI
NGREVMARVKFERDRFVGFVLEGVDEIPAEDKISGYAKFLNDNTLQVDDHTIITAKRIVIATGSRPAYPAVWNELGDRLI
INDDVFDWDDLPTSVAVFGPGVIGLELGQSLHRLGVETKLFGLGGQVGPVTDPEVMAYANKAFNEEFYLDADVKIESMKR
ISLNDDEDKVEIKFINKQGEQETILVEYVLAATGRRPNTDKLGLENTLVELDERGVPTADHYTLQTSLPTIFIAGDASNQ
LPLLHEAADQARIAGDNAGRFPEIRAGLRRSKISAVFSDPQIAMVGESYKEITTRLGTCGCFATGEVSFENQGRSRVMLR
NKGILHVYGEQGTGRFLGAEMMGPNAEHLAHLLAWAHQNKMTISEMLDMPFYHPVIEEGIRTALRDLNAKLHLGPEMIKH
CMDCGPGC
>Mature_488_residues
MKQVNVDVAVIGGGTAGLGSYRAAKAHTDSVVMIEGGPYGTTCARVGCMPSKLLIAAAESVHQIEKAPQFGIHPQGDIVI
NGREVMARVKFERDRFVGFVLEGVDEIPAEDKISGYAKFLNDNTLQVDDHTIITAKRIVIATGSRPAYPAVWNELGDRLI
INDDVFDWDDLPTSVAVFGPGVIGLELGQSLHRLGVETKLFGLGGQVGPVTDPEVMAYANKAFNEEFYLDADVKIESMKR
ISLNDDEDKVEIKFINKQGEQETILVEYVLAATGRRPNTDKLGLENTLVELDERGVPTADHYTLQTSLPTIFIAGDASNQ
LPLLHEAADQARIAGDNAGRFPEIRAGLRRSKISAVFSDPQIAMVGESYKEITTRLGTCGCFATGEVSFENQGRSRVMLR
NKGILHVYGEQGTGRFLGAEMMGPNAEHLAHLLAWAHQNKMTISEMLDMPFYHPVIEEGIRTALRDLNAKLHLGPEMIKH
CMDCGPGC

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransfer

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=481, Percent_Identity=27.2349272349272, Blast_Score=139, Evalue=5e-33,
Organism=Escherichia coli, GI1786307, Length=476, Percent_Identity=24.5798319327731, Blast_Score=131, Evalue=8e-32,
Organism=Escherichia coli, GI87082354, Length=490, Percent_Identity=25.1020408163265, Blast_Score=118, Evalue=9e-28,
Organism=Escherichia coli, GI1789915, Length=359, Percent_Identity=28.6908077994429, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI87081717, Length=462, Percent_Identity=23.1601731601732, Blast_Score=84, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI32565766, Length=479, Percent_Identity=26.5135699373695, Blast_Score=141, Evalue=8e-34,
Organism=Caenorhabditis elegans, GI71983419, Length=375, Percent_Identity=26.4, Blast_Score=88, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI71983429, Length=375, Percent_Identity=26.4, Blast_Score=88, Evalue=9e-18,
Organism=Saccharomyces cerevisiae, GI6321091, Length=488, Percent_Identity=27.8688524590164, Blast_Score=136, Evalue=8e-33,
Organism=Saccharomyces cerevisiae, GI6325166, Length=392, Percent_Identity=27.2959183673469, Blast_Score=92, Evalue=2e-19,
Organism=Drosophila melanogaster, GI21358499, Length=476, Percent_Identity=27.3109243697479, Blast_Score=133, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24640551, Length=499, Percent_Identity=24.8496993987976, Blast_Score=92, Evalue=5e-19,
Organism=Drosophila melanogaster, GI24640549, Length=499, Percent_Identity=24.8496993987976, Blast_Score=92, Evalue=6e-19,
Organism=Drosophila melanogaster, GI24640553, Length=499, Percent_Identity=24.8496993987976, Blast_Score=92, Evalue=7e-19,
Organism=Drosophila melanogaster, GI17737741, Length=499, Percent_Identity=22.4448897795591, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 53259; Mature: 53259

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQVNVDVAVIGGGTAGLGSYRAAKAHTDSVVMIEGGPYGTTCARVGCMPSKLLIAAAES
CCEEEEEEEEECCCCCCCCCCCCCCCCCCCEEEECCCCCCCHHHHHCCCCHHHHHHHHHH
VHQIEKAPQFGIHPQGDIVINGREVMARVKFERDRFVGFVLEGVDEIPAEDKISGYAKFL
HHHHHHCCCCCCCCCCCEEECCHHHEEEEHHHHHHHHHHHHHCHHHCCCCCHHHHHHHHH
NDNTLQVDDHTIITAKRIVIATGSRPAYPAVWNELGDRLIINDDVFDWDDLPTSVAVFGP
CCCEEEECCCEEEEEEEEEEEECCCCCCHHHHHHHCCEEEEECCCCCCCCCCCEEEEECC
GVIGLELGQSLHRLGVETKLFGLGGQVGPVTDPEVMAYANKAFNEEFYLDADVKIESMKR
CEEEHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHCCCEEEEECCCEEEEEEE
ISLNDDEDKVEIKFINKQGEQETILVEYVLAATGRRPNTDKLGLENTLVELDERGVPTAD
ECCCCCCCEEEEEEECCCCCCCEEHHEEHHHHCCCCCCCCCCCCHHHHHHHHCCCCCCCC
HYTLQTSLPTIFIAGDASNQLPLLHEAADQARIAGDNAGRFPEIRAGLRRSKISAVFSDP
CEEEEECCCEEEEECCCCCCCCHHHHHHHHHEECCCCCCCCHHHHHHHHHHHHHHEECCC
QIAMVGESYKEITTRLGTCGCFATGEVSFENQGRSRVMLRNKGILHVYGEQGTGRFLGAE
CEEEECCHHHHHHHHHCCCEEEEECEEEECCCCCEEEEEECCCEEEEECCCCCCCEEEHH
MMGPNAEHLAHLLAWAHQNKMTISEMLDMPFYHPVIEEGIRTALRDLNAKLHLGPEMIKH
HCCCCHHHHHHHHHHHHCCCCCHHHHHCCCCCHHHHHHHHHHHHHHCCCEEEECHHHHHH
CMDCGPGC
HHHCCCCC
>Mature Secondary Structure
MKQVNVDVAVIGGGTAGLGSYRAAKAHTDSVVMIEGGPYGTTCARVGCMPSKLLIAAAES
CCEEEEEEEEECCCCCCCCCCCCCCCCCCCEEEECCCCCCCHHHHHCCCCHHHHHHHHHH
VHQIEKAPQFGIHPQGDIVINGREVMARVKFERDRFVGFVLEGVDEIPAEDKISGYAKFL
HHHHHHCCCCCCCCCCCEEECCHHHEEEEHHHHHHHHHHHHHCHHHCCCCCHHHHHHHHH
NDNTLQVDDHTIITAKRIVIATGSRPAYPAVWNELGDRLIINDDVFDWDDLPTSVAVFGP
CCCEEEECCCEEEEEEEEEEEECCCCCCHHHHHHHCCEEEEECCCCCCCCCCCEEEEECC
GVIGLELGQSLHRLGVETKLFGLGGQVGPVTDPEVMAYANKAFNEEFYLDADVKIESMKR
CEEEHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHCCCEEEEECCCEEEEEEE
ISLNDDEDKVEIKFINKQGEQETILVEYVLAATGRRPNTDKLGLENTLVELDERGVPTAD
ECCCCCCCEEEEEEECCCCCCCEEHHEEHHHHCCCCCCCCCCCCHHHHHHHHCCCCCCCC
HYTLQTSLPTIFIAGDASNQLPLLHEAADQARIAGDNAGRFPEIRAGLRRSKISAVFSDP
CEEEEECCCEEEEECCCCCCCCHHHHHHHHHEECCCCCCCCHHHHHHHHHHHHHHEECCC
QIAMVGESYKEITTRLGTCGCFATGEVSFENQGRSRVMLRNKGILHVYGEQGTGRFLGAE
CEEEECCHHHHHHHHHCCCEEEEECEEEECCCCCEEEEEECCCEEEEECCCCCCCEEEHH
MMGPNAEHLAHLLAWAHQNKMTISEMLDMPFYHPVIEEGIRTALRDLNAKLHLGPEMIKH
HCCCCHHHHHHHHHHHHCCCCCHHHHHCCCCCHHHHHHHHHHHHHHCCCEEEECHHHHHH
CMDCGPGC
HHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362 [H]