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The map label for this gene is ilvI [H]

Identifier: 209696113

GI number: 209696113

Start: 2929906

End: 2931627

Strand: Reverse

Name: ilvI [H]

Synonym: VSAL_I2706

Alternate gene names: 209696113

Gene position: 2931627-2929906 (Counterclockwise)

Preceding gene: 209696114

Following gene: 209696112

Centisome position: 88.16

GC content: 40.42

Gene sequence:

>1722_bases
ATGGAAATGTTATCTGGCTCAGAGATGATCGTACAGTCTTTGATTGATGAAGGTGTAGAGCACATATTCGGATACCCTGG
CGGTTCTGTTCTTGATATTTATGATGCGTTGCATATGAAAAGCGACGATATTCAACATGTATTAGTGAGACATGAGCAGG
CAGCAACACACATGGCTGATGGTTATGCACGTGCAACAGGTAAACCTGGCGTGGTTCTTGTGTGCTCAGGCCCGGGAGCA
ACGAACACGATTACAGGAATAGCAACGGCCTATATGGATTCTATTCCAATGATTGTGCTATCTGGCAACGTACCAAACAA
CATGATTGGCACTGATGCCTTTCAAGAGTGCGACATGGTAGGCGTCTCTCGCCCGATAGTTAAGCATAGTTTTTTAGTTA
AAAAAGCCGAAGACATTCCTGAAATTATAAAAAAAGCATTTTATATTGCATCGACAGGCCGACCAGGCCCTGTTGTTATT
GATATTCCTAAAGATGTCATGAGCCCTGCATTTACATTCCCTTATCAGTATCCTGAAGCGGTTTCGTTACGTTCTTATAA
TCCCACAATGAATGGCCATAAAGGGCAAATTAAAAAAGGGCTAAAAGCATTACTTTCTGCTAAGAAGCCTGTCCTTTATA
TTGGTGGTGGTGCAATTATTTCTTCCGCAGATGCTCAAATATTAACGTTAGCTGAAGCGTTAAACTTACCCGTCATAAGT
ACCTTAATGGGGCTAGGAGCATTTCCTGGTACGCATAAGAATGCATTAGGTATGTTGGGTATGCATGGTACGTATGAAGC
AAATATGACAATGCACAATGCAGATCTAATTTTTGGTATTGGCGTTAGATTCGATGATCGAACGACGAATAACTTAGAAA
AATATTGTCCAGATGCAACCATTATGCATATTGATATCGACCCTTCTTCTATTTCTAAAAATGTTCGAGTGGATTTACCT
ATTGTTGGTTCTGCTGATATTGTCCTCGACTCAATGCTGTCATTAATGAATGAACAAGATGGACATAATGATGCCGAAGC
GATGGAAGCATGGTGGAAAGATATCGCAGGGTGGCGTGCTCGTAATTGTTTAGCGTATAAAAAATTACCGGATAAAATTA
AACCGCAACAAGTGATAGAAACACTCTATAAATTAACCAATGGTGATGCTTATGTTGCTTCTGATGTAGGTCAGCATCAA
ATGTTCGCGGCATTATATTATCCATTTGATAAACCTCGTCGTTGGATAAATTCGGGTGGTTTGGGGACAATGGGCTTTGG
TTTACCTGCGGCTTTAGGGGTTAAATTTACGCATCCTGATGACGTTGTTGTGTGTGTTACCGGAGATGGCAGTATTCAGA
TGAATATCCAAGAGTTATCAACGGCGCTTCAATACGATATTCCGGTAAAAATTATTAATATTAATAACCGATTTTTAGGC
ATGGTTAAACAATGGCAGGACATCATTTATCAAGGCAGACACTCAAACTCTTACATGAGCTCTGTTCCTGATTTTGCAGC
GATTGCAGAAGCTTATGGACACGTTGGAATCCGAATTAATCACCCTGATGAATTGGAATCTGGTCTAGAAAAAGCATTAG
CAATGAAGGATCGACTGGTCTTTGTTGATATTAATGTTGACGAAACGGAACATGTTTACCCAATGCAAATTAAAGGGGAA
GCAATGGACAAGATGTGGCTAAGTAAGACGGAGAGAACGTAA

Upstream 100 bases:

>100_bases
AATTCGGTTTAGGCTAAAAATAAGCCCTAACTATGTAAAACCAATAATTTGTAGATAATTCAAATCTTCTATTTGAGGTT
TTCAGCGTCTGGAGATAAAT

Downstream 100 bases:

>100_bases
TGAGACATATAATATCACTGCTATTAGAAAATGAGTCAGGGGCGTTATCTCGTGTTGTAGGTCTGTTCTCTCAACGAGGT
TATAATATTGAGTCATTAAC

Product: acetolactate synthase 3 catalytic subunit

Products: NA

Alternate protein names: AHAS-III; ALS-III; Acetohydroxy-acid synthase III large subunit [H]

Number of amino acids: Translated: 573; Mature: 573

Protein sequence:

>573_residues
MEMLSGSEMIVQSLIDEGVEHIFGYPGGSVLDIYDALHMKSDDIQHVLVRHEQAATHMADGYARATGKPGVVLVCSGPGA
TNTITGIATAYMDSIPMIVLSGNVPNNMIGTDAFQECDMVGVSRPIVKHSFLVKKAEDIPEIIKKAFYIASTGRPGPVVI
DIPKDVMSPAFTFPYQYPEAVSLRSYNPTMNGHKGQIKKGLKALLSAKKPVLYIGGGAIISSADAQILTLAEALNLPVIS
TLMGLGAFPGTHKNALGMLGMHGTYEANMTMHNADLIFGIGVRFDDRTTNNLEKYCPDATIMHIDIDPSSISKNVRVDLP
IVGSADIVLDSMLSLMNEQDGHNDAEAMEAWWKDIAGWRARNCLAYKKLPDKIKPQQVIETLYKLTNGDAYVASDVGQHQ
MFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKFTHPDDVVVCVTGDGSIQMNIQELSTALQYDIPVKIININNRFLG
MVKQWQDIIYQGRHSNSYMSSVPDFAAIAEAYGHVGIRINHPDELESGLEKALAMKDRLVFVDINVDETEHVYPMQIKGE
AMDKMWLSKTERT

Sequences:

>Translated_573_residues
MEMLSGSEMIVQSLIDEGVEHIFGYPGGSVLDIYDALHMKSDDIQHVLVRHEQAATHMADGYARATGKPGVVLVCSGPGA
TNTITGIATAYMDSIPMIVLSGNVPNNMIGTDAFQECDMVGVSRPIVKHSFLVKKAEDIPEIIKKAFYIASTGRPGPVVI
DIPKDVMSPAFTFPYQYPEAVSLRSYNPTMNGHKGQIKKGLKALLSAKKPVLYIGGGAIISSADAQILTLAEALNLPVIS
TLMGLGAFPGTHKNALGMLGMHGTYEANMTMHNADLIFGIGVRFDDRTTNNLEKYCPDATIMHIDIDPSSISKNVRVDLP
IVGSADIVLDSMLSLMNEQDGHNDAEAMEAWWKDIAGWRARNCLAYKKLPDKIKPQQVIETLYKLTNGDAYVASDVGQHQ
MFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKFTHPDDVVVCVTGDGSIQMNIQELSTALQYDIPVKIININNRFLG
MVKQWQDIIYQGRHSNSYMSSVPDFAAIAEAYGHVGIRINHPDELESGLEKALAMKDRLVFVDINVDETEHVYPMQIKGE
AMDKMWLSKTERT
>Mature_573_residues
MEMLSGSEMIVQSLIDEGVEHIFGYPGGSVLDIYDALHMKSDDIQHVLVRHEQAATHMADGYARATGKPGVVLVCSGPGA
TNTITGIATAYMDSIPMIVLSGNVPNNMIGTDAFQECDMVGVSRPIVKHSFLVKKAEDIPEIIKKAFYIASTGRPGPVVI
DIPKDVMSPAFTFPYQYPEAVSLRSYNPTMNGHKGQIKKGLKALLSAKKPVLYIGGGAIISSADAQILTLAEALNLPVIS
TLMGLGAFPGTHKNALGMLGMHGTYEANMTMHNADLIFGIGVRFDDRTTNNLEKYCPDATIMHIDIDPSSISKNVRVDLP
IVGSADIVLDSMLSLMNEQDGHNDAEAMEAWWKDIAGWRARNCLAYKKLPDKIKPQQVIETLYKLTNGDAYVASDVGQHQ
MFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKFTHPDDVVVCVTGDGSIQMNIQELSTALQYDIPVKIININNRFLG
MVKQWQDIIYQGRHSNSYMSSVPDFAAIAEAYGHVGIRINHPDELESGLEKALAMKDRLVFVDINVDETEHVYPMQIKGE
AMDKMWLSKTERT

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=603, Percent_Identity=27.1973466003317, Blast_Score=201, Evalue=2e-51,
Organism=Homo sapiens, GI21361361, Length=508, Percent_Identity=25.3937007874016, Blast_Score=141, Evalue=1e-33,
Organism=Escherichia coli, GI87081685, Length=575, Percent_Identity=70.9565217391304, Blast_Score=890, Evalue=0.0,
Organism=Escherichia coli, GI1790104, Length=563, Percent_Identity=41.0301953818828, Blast_Score=432, Evalue=1e-122,
Organism=Escherichia coli, GI1786717, Length=535, Percent_Identity=34.7663551401869, Blast_Score=311, Evalue=8e-86,
Organism=Escherichia coli, GI1787096, Length=555, Percent_Identity=28.4684684684685, Blast_Score=195, Evalue=8e-51,
Organism=Escherichia coli, GI1788716, Length=577, Percent_Identity=23.9168110918544, Blast_Score=174, Evalue=1e-44,
Organism=Caenorhabditis elegans, GI17531299, Length=551, Percent_Identity=26.497277676951, Blast_Score=170, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI17531301, Length=551, Percent_Identity=26.497277676951, Blast_Score=170, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI17542570, Length=571, Percent_Identity=25.7443082311734, Blast_Score=139, Evalue=4e-33,
Organism=Saccharomyces cerevisiae, GI6323755, Length=583, Percent_Identity=41.1663807890223, Blast_Score=433, Evalue=1e-122,
Organism=Saccharomyces cerevisiae, GI6320816, Length=547, Percent_Identity=24.3144424131627, Blast_Score=129, Evalue=1e-30,
Organism=Drosophila melanogaster, GI19922626, Length=595, Percent_Identity=23.8655462184874, Blast_Score=180, Evalue=2e-45,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 62755; Mature: 62755

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEMLSGSEMIVQSLIDEGVEHIFGYPGGSVLDIYDALHMKSDDIQHVLVRHEQAATHMAD
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHC
GYARATGKPGVVLVCSGPGATNTITGIATAYMDSIPMIVLSGNVPNNMIGTDAFQECDMV
CHHCCCCCCCEEEEECCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHCCCC
GVSRPIVKHSFLVKKAEDIPEIIKKAFYIASTGRPGPVVIDIPKDVMSPAFTFPYQYPEA
CCCCHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCEEEECCHHHCCCCEECCCCCCCC
VSLRSYNPTMNGHKGQIKKGLKALLSAKKPVLYIGGGAIISSADAQILTLAEALNLPVIS
EEEECCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCEECCCCCHHHHHHHHHCCHHHH
TLMGLGAFPGTHKNALGMLGMHGTYEANMTMHNADLIFGIGVRFDDRTTNNLEKYCPDAT
HHHHCCCCCCCCCCCCEEECCCCEEECCEEEECCCEEEEEEEEECCCCCCCHHHHCCCCE
IMHIDIDPSSISKNVRVDLPIVGSADIVLDSMLSLMNEQDGHNDAEAMEAWWKDIAGWRA
EEEEECCHHHCCCCEEEEEEECCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHH
RNCLAYKKLPDKIKPQQVIETLYKLTNGDAYVASDVGQHQMFAALYYPFDKPRRWINSGG
CCCHHHHCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCHHEEEEEECCCHHHHHHHCCCC
LGTMGFGLPAALGVKFTHPDDVVVCVTGDGSIQMNIQELSTALQYDIPVKIININNRFLG
CCCCCCCCHHHHCEEEECCCCEEEEEECCCCEEEEHHHHHHHHEECCCEEEEEECCHHHH
MVKQWQDIIYQGRHSNSYMSSVPDFAAIAEAYGHVGIRINHPDELESGLEKALAMKDRLV
HHHHHHHHHHCCCCCCCHHHHCCHHHHHHHHHCCEEEEECCHHHHHHHHHHHHHHHCCEE
FVDINVDETEHVYPMQIKGEAMDKMWLSKTERT
EEEECCCCCCCEEEEEECCCHHHHHHHHHCCCC
>Mature Secondary Structure
MEMLSGSEMIVQSLIDEGVEHIFGYPGGSVLDIYDALHMKSDDIQHVLVRHEQAATHMAD
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHC
GYARATGKPGVVLVCSGPGATNTITGIATAYMDSIPMIVLSGNVPNNMIGTDAFQECDMV
CHHCCCCCCCEEEEECCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHCCCC
GVSRPIVKHSFLVKKAEDIPEIIKKAFYIASTGRPGPVVIDIPKDVMSPAFTFPYQYPEA
CCCCHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCEEEECCHHHCCCCEECCCCCCCC
VSLRSYNPTMNGHKGQIKKGLKALLSAKKPVLYIGGGAIISSADAQILTLAEALNLPVIS
EEEECCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCEECCCCCHHHHHHHHHCCHHHH
TLMGLGAFPGTHKNALGMLGMHGTYEANMTMHNADLIFGIGVRFDDRTTNNLEKYCPDAT
HHHHCCCCCCCCCCCCEEECCCCEEECCEEEECCCEEEEEEEEECCCCCCCHHHHCCCCE
IMHIDIDPSSISKNVRVDLPIVGSADIVLDSMLSLMNEQDGHNDAEAMEAWWKDIAGWRA
EEEEECCHHHCCCCEEEEEEECCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHH
RNCLAYKKLPDKIKPQQVIETLYKLTNGDAYVASDVGQHQMFAALYYPFDKPRRWINSGG
CCCHHHHCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCHHEEEEEECCCHHHHHHHCCCC
LGTMGFGLPAALGVKFTHPDDVVVCVTGDGSIQMNIQELSTALQYDIPVKIININNRFLG
CCCCCCCCHHHHCEEEECCCCEEEEEECCCCEEEEHHHHHHHHEECCCEEEEEECCHHHH
MVKQWQDIIYQGRHSNSYMSSVPDFAAIAEAYGHVGIRINHPDELESGLEKALAMKDRLV
HHHHHHHHHHCCCCCCCHHHHCCHHHHHHHHHCCEEEEECCHHHHHHHHHHHHHHHCCEE
FVDINVDETEHVYPMQIKGEAMDKMWLSKTERT
EEEECCCCCCCEEEEEECCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6308579; 1630901; 9278503; 3891724; 9298646 [H]