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The map label for this gene is cca

Identifier: 209696097

GI number: 209696097

Start: 2911481

End: 2912704

Strand: Direct

Name: cca

Synonym: VSAL_I2689

Alternate gene names: 209696097

Gene position: 2911481-2912704 (Clockwise)

Preceding gene: 209696091

Following gene: 209696101

Centisome position: 87.56

GC content: 40.6

Gene sequence:

>1224_bases
ATGCAAATTTATTTAGTAGGTGGTGCAGTTCGAGATTCACTCCTCAATATTGACGTAAAAGATAAAGATTGGGTCGTTGT
TGGTTCTACACCCACTCACATGAATTCGCTTGGCTACCAAAGCGTAGGGCAAGACTTTCCTGTTTTCTTACACCCAAGAA
CAAAAGAAGAATACGCCTTAGCCAGAACAGAACGAAAAAGTGGTCAAGGATATAAAGGATTCACTTGTTACGCAGAAGCA
GATGTCACATTAGAAGAAGATTTGTTGCGTCGAGATTTGACGATTAATGCGATAGCTCAATCAGATAAGGGCCTATTAAT
TGATCCTTACCATGGGCAAGAAGATTTAAAGAATCGGTTATTACGCCATGTATCCAATGCCTTTGTCGAAGACCCGTTAC
GGGTACTACGAGTTGCTCGTTTTGCCGCTCGCTTTCATTATCTTGGTTTTACAATTGCACCAGAAACAATGCAATTAATG
AAAACACTTGTTTCGAGTGGCGAGCTCTCTCATTTAACCCCAGAACGAGTATGGCAAGAATGGGAAAAATCATTATCAAG
TAAACACCCAAATATTTTTCTTTCTGTACTCAAAGAATGTGGTGCATTAGCGGTCGTATTGCCGGAAATTGATGCTTTAT
TTGGCGTTCCTCAACCAGAACAATGGCACCCAGAAATTGATACTGGAATGCATACATTGATGGTTTCTCAGCAAGCATCA
TTACTAAGTACCTCACTTCCAATACGCTTTGCAGCGCAAGTTCATGATTTAGGTAAGGGGATTACTCCTGAATCAGAATG
GCCAAGCCACAAAATGCATTGCCATACAGGGATAAAAGTAATCAAGAATTTGTGTAAGCGAGTTCGAGTCCCTAATGAAT
ACAAAGAGCTAGCCTTATTGGTTTGTGAGCATCACACAAATGTCCACAGAGCTTCAGAACTACGAGCACAAACCTTTATC
AAGATATTCGATAAAATGGACTTATGGCGTAAACCAGAACGTTTAGAATCGATTCTATTGTGCTGCCAAGCCGATCATGC
AGGGCGACTAGGGTTAGAGTTAAATCCATACCCTCAAAAAGAACGAATGGAATCCGCATTTAATGCAGCACAACATGTAG
AGGTAAAAGAAGTGGTCGCAGCAGGGTTTAAAGGACCAGAAATCAGAGATGAATTAACCAAGAGAAGAATTGAAGCTGTC
AAAGTGGCACTTTTAATAAACTAG

Upstream 100 bases:

>100_bases
TCTTCGAATAAAAAGTCGAAATATTATCATTGCACGGTATCATTGGAAGTCAGAAAGTAAAGAAAGTTCTCTTTATCTTG
ATTGTGTGTAGGAAAAACAG

Downstream 100 bases:

>100_bases
AATTAAAAAGTGGTTGTTAATAAGTTCATCACTTACAACAACCACCTTAATCTAGACTCTAATTATTACGTTAACGCTAA
CCAAGCAAGTAGGCTCGTCC

Product: multifunctional tRNA nucleotidyl transferase/2'3'-cyclic phosphodiesterase/2'nucleotidase/phosphatase

Products: NA

Alternate protein names: CCA-adding enzyme; tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl-transferase; tRNA nucleotidyltransferase; tRNA-NT; 2'-nucleotidase; 2',3'-cyclic phosphodiesterase; Phosphatase

Number of amino acids: Translated: 407; Mature: 407

Protein sequence:

>407_residues
MQIYLVGGAVRDSLLNIDVKDKDWVVVGSTPTHMNSLGYQSVGQDFPVFLHPRTKEEYALARTERKSGQGYKGFTCYAEA
DVTLEEDLLRRDLTINAIAQSDKGLLIDPYHGQEDLKNRLLRHVSNAFVEDPLRVLRVARFAARFHYLGFTIAPETMQLM
KTLVSSGELSHLTPERVWQEWEKSLSSKHPNIFLSVLKECGALAVVLPEIDALFGVPQPEQWHPEIDTGMHTLMVSQQAS
LLSTSLPIRFAAQVHDLGKGITPESEWPSHKMHCHTGIKVIKNLCKRVRVPNEYKELALLVCEHHTNVHRASELRAQTFI
KIFDKMDLWRKPERLESILLCCQADHAGRLGLELNPYPQKERMESAFNAAQHVEVKEVVAAGFKGPEIRDELTKRRIEAV
KVALLIN

Sequences:

>Translated_407_residues
MQIYLVGGAVRDSLLNIDVKDKDWVVVGSTPTHMNSLGYQSVGQDFPVFLHPRTKEEYALARTERKSGQGYKGFTCYAEA
DVTLEEDLLRRDLTINAIAQSDKGLLIDPYHGQEDLKNRLLRHVSNAFVEDPLRVLRVARFAARFHYLGFTIAPETMQLM
KTLVSSGELSHLTPERVWQEWEKSLSSKHPNIFLSVLKECGALAVVLPEIDALFGVPQPEQWHPEIDTGMHTLMVSQQAS
LLSTSLPIRFAAQVHDLGKGITPESEWPSHKMHCHTGIKVIKNLCKRVRVPNEYKELALLVCEHHTNVHRASELRAQTFI
KIFDKMDLWRKPERLESILLCCQADHAGRLGLELNPYPQKERMESAFNAAQHVEVKEVVAAGFKGPEIRDELTKRRIEAV
KVALLIN
>Mature_407_residues
MQIYLVGGAVRDSLLNIDVKDKDWVVVGSTPTHMNSLGYQSVGQDFPVFLHPRTKEEYALARTERKSGQGYKGFTCYAEA
DVTLEEDLLRRDLTINAIAQSDKGLLIDPYHGQEDLKNRLLRHVSNAFVEDPLRVLRVARFAARFHYLGFTIAPETMQLM
KTLVSSGELSHLTPERVWQEWEKSLSSKHPNIFLSVLKECGALAVVLPEIDALFGVPQPEQWHPEIDTGMHTLMVSQQAS
LLSTSLPIRFAAQVHDLGKGITPESEWPSHKMHCHTGIKVIKNLCKRVRVPNEYKELALLVCEHHTNVHRASELRAQTFI
KIFDKMDLWRKPERLESILLCCQADHAGRLGLELNPYPQKERMESAFNAAQHVEVKEVVAAGFKGPEIRDELTKRRIEAV
KVALLIN

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 1 subfamily

Homologues:

Organism=Homo sapiens, GI155030240, Length=206, Percent_Identity=31.5533980582524, Blast_Score=75, Evalue=2e-13,
Organism=Escherichia coli, GI1789436, Length=400, Percent_Identity=61.75, Blast_Score=520, Evalue=1e-149,
Organism=Escherichia coli, GI87081691, Length=339, Percent_Identity=27.7286135693215, Blast_Score=80, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6321016, Length=124, Percent_Identity=40.3225806451613, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CCA_ALISL (B6EM10)

Other databases:

- EMBL:   FM178379
- RefSeq:   YP_002264027.1
- GeneID:   6988459
- GenomeReviews:   FM178379_GR
- KEGG:   vsa:VSAL_I2689
- HOGENOM:   HBG635063
- OMA:   GHGQKGL
- ProtClustDB:   PRK10885
- HAMAP:   MF_01261
- InterPro:   IPR012006
- InterPro:   IPR003607
- InterPro:   IPR002646
- PIRSF:   PIRSF000813
- SMART:   SM00471

Pfam domain/function: PF01743 PolyA_pol

EC number: =2.7.7.21; =2.7.7.25

Molecular weight: Translated: 46148; Mature: 46148

Theoretical pI: Translated: 7.30; Mature: 7.30

Prosite motif: NA

Important sites: BINDING 8-8 BINDING 11-11 BINDING 91-91 BINDING 137-137 BINDING 140-140

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIYLVGGAVRDSLLNIDVKDKDWVVVGSTPTHMNSLGYQSVGQDFPVFLHPRTKEEYAL
CEEEEECCHHHHHHEEEEECCCCEEEECCCCCHHHHCCHHHCCCCCCEEECCCCCHHHHH
ARTERKSGQGYKGFTCYAEADVTLEEDLLRRDLTINAIAQSDKGLLIDPYHGQEDLKNRL
HHHHHHCCCCCCCEEEEEECCCCHHHHHHHHCCEEEEEECCCCCCEECCCCCHHHHHHHH
LRHVSNAFVEDPLRVLRVARFAARFHYLGFTIAPETMQLMKTLVSSGELSHLTPERVWQE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEECHHHHHHHHHHHCCCCCCCCCHHHHHHH
WEKSLSSKHPNIFLSVLKECGALAVVLPEIDALFGVPQPEQWHPEIDTGMHTLMVSQQAS
HHHHHHCCCCHHHHHHHHHCCCEEEEHHHHHHHHCCCCCCCCCCCHHHCHHHHHHHHHHH
LLSTSLPIRFAAQVHDLGKGITPESEWPSHKMHCHTGIKVIKNLCKRVRVPNEYKELALL
HHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
VCEHHTNVHRASELRAQTFIKIFDKMDLWRKPERLESILLCCQADHAGRLGLELNPYPQK
HHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCH
ERMESAFNAAQHVEVKEVVAAGFKGPEIRDELTKRRIEAVKVALLIN
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHEEEEEC
>Mature Secondary Structure
MQIYLVGGAVRDSLLNIDVKDKDWVVVGSTPTHMNSLGYQSVGQDFPVFLHPRTKEEYAL
CEEEEECCHHHHHHEEEEECCCCEEEECCCCCHHHHCCHHHCCCCCCEEECCCCCHHHHH
ARTERKSGQGYKGFTCYAEADVTLEEDLLRRDLTINAIAQSDKGLLIDPYHGQEDLKNRL
HHHHHHCCCCCCCEEEEEECCCCHHHHHHHHCCEEEEEECCCCCCEECCCCCHHHHHHHH
LRHVSNAFVEDPLRVLRVARFAARFHYLGFTIAPETMQLMKTLVSSGELSHLTPERVWQE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEECHHHHHHHHHHHCCCCCCCCCHHHHHHH
WEKSLSSKHPNIFLSVLKECGALAVVLPEIDALFGVPQPEQWHPEIDTGMHTLMVSQQAS
HHHHHHCCCCHHHHHHHHHCCCEEEEHHHHHHHHCCCCCCCCCCCHHHCHHHHHHHHHHH
LLSTSLPIRFAAQVHDLGKGITPESEWPSHKMHCHTGIKVIKNLCKRVRVPNEYKELALL
HHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
VCEHHTNVHRASELRAQTFIKIFDKMDLWRKPERLESILLCCQADHAGRLGLELNPYPQK
HHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCH
ERMESAFNAAQHVEVKEVVAAGFKGPEIRDELTKRRIEAVKVALLIN
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA