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The map label for this gene is murE [H]

Identifier: 209696057

GI number: 209696057

Start: 2867283

End: 2868764

Strand: Reverse

Name: murE [H]

Synonym: VSAL_I2649

Alternate gene names: 209696057

Gene position: 2868764-2867283 (Counterclockwise)

Preceding gene: 209696058

Following gene: 209696056

Centisome position: 86.27

GC content: 41.5

Gene sequence:

>1482_bases
ATGATGCAGTTATCTGAATTATTGTTGCCTTGGTGTGAATTAAATCAAGTCGTAGAAATATCATCCATGACATTGGACAG
TCGTAAAGTAACCACTGGTTGCCTATTTGTTGCGATTAAAGGCCACGCGGTCGATGGCCGACAATTTATCCCTGTTGCGA
TTAAGCAAGGTGCATCGGCGGTATTGGCTCAAAGTGATGAGGTTAATAAGACGGGAACCATTAGTTTTCTCGATCAGACC
CCTATTATTTATCTCGATGCGCTATCGATAAAACTCAGCGCGGTCGCTGGGCGTTTTTATCACGCTGAACAAAGAAAAAT
GCAGTTGGTTGGCATTACTGGAACCAATGGAAAAACAACCATATCGCAAATTATTGCTCAATGGGCCACATTACTTGGTA
AACCAGCTGGAGTCATGGGGACCACGGGCAATGGATTATTAACCGATCTGAAACCTGCGTTAAATACGACGGGTAGCCCA
ATCGAGATCCAACAGACATTAACGGATCTTGAAAATATAGGGGCAAAGCTAACCGCATTAGAAATATCTTCTCATGGGTT
GGTTCAAGGGCGTGTTAATGCCTTGAATTTTGATGCTGCTGTATTCACTAATTTAAGTCGTGATCACTTAGATTATCATG
GTGATATGGCAAATTATGCTGAAGCAAAAAAACTGTTGTTTACCTCTCACAGTCCTAAATATTCAATCATTAATGCCGAT
GATACTGTGGGTGCTTCTTGGTTAAAAGAATTGCCTAATGCTGTCGGGGTATCATTGAACGAAGGTAATCTTTCTTTTCA
TTCAGGGAAAAAAGTGTTTGCAACTTCTGTCATTTATTCTACGGATGGAATTCAAATATCTCTGAATTCTGATTGGGGAA
CCGCGGATCTTTCTGTTCCGTTGATCGGTGCGTTTAATGCCAATAATATTTTAGTTGCTTTTGCTACTCTTCTATCGCTT
GGTTACGACATAACTGAATTAGCGGACGCCGCTCCTAAATTAACCGCGGTTATTGGACGAATGGAGCTTTTCAGTTCTGA
AGGTAATGCGAAAGTGGTTGTGGACTATGCACATACTCCAGATGCTTTAGATAAAGCCTTGCAAGCCCTTCGAGTTCATT
GTGAAGGCAAGCTGTGGTGTATATTTGGTTGTGGCGGTGATCGAGATACGGGCAAGCGTCCGATGATGGCTAGTATTGCT
GAGCAACAAGCTGATGTGATTATTTTAAGTGATGATAATCCTCGTTCAGAAGATCCAAGCTTGATTACTAAAGACATGTT
AGCTGGTTTGAACCAACCAGACATTGCACACGTGATACATCAGCGTTTTGATGCATGCCAGTTTGCTCTCTCTCAAGCAA
GTAAGCATGATATTATTTTATTGGCAGGCAAGGGGCATGAAGAATATCAAGTGCTTGCTACAGAAACGATTCATTATTCT
GATCGTGAAACTGCGCAGAAATTATTAGGACTATCATCATGA

Upstream 100 bases:

>100_bases
CCTATTTTTGGTCAGGTGATGAAAGATGCATTACAAATATTAAATGTAGCCCCAGACGAAAGTACCGTAAAACATATCGA
TGTATTGAATTAGGAAAAGG

Downstream 100 bases:

>100_bases
TCTTAATGTCATTGAATGAAGTGGCGAACATTTGTCAGGGACGTTTGAATGGGGTAAATACCTCGATTTCAAGCGTGTCG
ACTGACAGTCGAAATATTGA

Product: UDP-N-acetylmuramoylalanyl-D-glutamate--2,6-dia minopimelate ligase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase [H]

Number of amino acids: Translated: 493; Mature: 493

Protein sequence:

>493_residues
MMQLSELLLPWCELNQVVEISSMTLDSRKVTTGCLFVAIKGHAVDGRQFIPVAIKQGASAVLAQSDEVNKTGTISFLDQT
PIIYLDALSIKLSAVAGRFYHAEQRKMQLVGITGTNGKTTISQIIAQWATLLGKPAGVMGTTGNGLLTDLKPALNTTGSP
IEIQQTLTDLENIGAKLTALEISSHGLVQGRVNALNFDAAVFTNLSRDHLDYHGDMANYAEAKKLLFTSHSPKYSIINAD
DTVGASWLKELPNAVGVSLNEGNLSFHSGKKVFATSVIYSTDGIQISLNSDWGTADLSVPLIGAFNANNILVAFATLLSL
GYDITELADAAPKLTAVIGRMELFSSEGNAKVVVDYAHTPDALDKALQALRVHCEGKLWCIFGCGGDRDTGKRPMMASIA
EQQADVIILSDDNPRSEDPSLITKDMLAGLNQPDIAHVIHQRFDACQFALSQASKHDIILLAGKGHEEYQVLATETIHYS
DRETAQKLLGLSS

Sequences:

>Translated_493_residues
MMQLSELLLPWCELNQVVEISSMTLDSRKVTTGCLFVAIKGHAVDGRQFIPVAIKQGASAVLAQSDEVNKTGTISFLDQT
PIIYLDALSIKLSAVAGRFYHAEQRKMQLVGITGTNGKTTISQIIAQWATLLGKPAGVMGTTGNGLLTDLKPALNTTGSP
IEIQQTLTDLENIGAKLTALEISSHGLVQGRVNALNFDAAVFTNLSRDHLDYHGDMANYAEAKKLLFTSHSPKYSIINAD
DTVGASWLKELPNAVGVSLNEGNLSFHSGKKVFATSVIYSTDGIQISLNSDWGTADLSVPLIGAFNANNILVAFATLLSL
GYDITELADAAPKLTAVIGRMELFSSEGNAKVVVDYAHTPDALDKALQALRVHCEGKLWCIFGCGGDRDTGKRPMMASIA
EQQADVIILSDDNPRSEDPSLITKDMLAGLNQPDIAHVIHQRFDACQFALSQASKHDIILLAGKGHEEYQVLATETIHYS
DRETAQKLLGLSS
>Mature_493_residues
MMQLSELLLPWCELNQVVEISSMTLDSRKVTTGCLFVAIKGHAVDGRQFIPVAIKQGASAVLAQSDEVNKTGTISFLDQT
PIIYLDALSIKLSAVAGRFYHAEQRKMQLVGITGTNGKTTISQIIAQWATLLGKPAGVMGTTGNGLLTDLKPALNTTGSP
IEIQQTLTDLENIGAKLTALEISSHGLVQGRVNALNFDAAVFTNLSRDHLDYHGDMANYAEAKKLLFTSHSPKYSIINAD
DTVGASWLKELPNAVGVSLNEGNLSFHSGKKVFATSVIYSTDGIQISLNSDWGTADLSVPLIGAFNANNILVAFATLLSL
GYDITELADAAPKLTAVIGRMELFSSEGNAKVVVDYAHTPDALDKALQALRVHCEGKLWCIFGCGGDRDTGKRPMMASIA
EQQADVIILSDDNPRSEDPSLITKDMLAGLNQPDIAHVIHQRFDACQFALSQASKHDIILLAGKGHEEYQVLATETIHYS
DRETAQKLLGLSS

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan [H]

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786273, Length=489, Percent_Identity=51.9427402862986, Blast_Score=479, Evalue=1e-136,
Organism=Escherichia coli, GI1786274, Length=327, Percent_Identity=25.9938837920489, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.13 [H]

Molecular weight: Translated: 53007; Mature: 53007

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMQLSELLLPWCELNQVVEISSMTLDSRKVTTGCLFVAIKGHAVDGRQFIPVAIKQGASA
CCCHHHHHCCHHHHCCEEEEHHEEECCCEEEEEEEEEEEECCCCCCCEEEEHEECCCCCE
VLAQSDEVNKTGTISFLDQTPIIYLDALSIKLSAVAGRFYHAEQRKMQLVGITGTNGKTT
EEECCCCCCCCEEEEEECCCCEEEEEEEEEEEEHHHHHHHCCCCCEEEEEEEECCCCCHH
ISQIIAQWATLLGKPAGVMGTTGNGLLTDLKPALNTTGSPIEIQQTLTDLENIGAKLTAL
HHHHHHHHHHHHCCCCCCEECCCCCCHHCCCHHHCCCCCCEEHHHHHHHHHHCCCEEEEE
EISSHGLVQGRVNALNFDAAVFTNLSRDHLDYHGDMANYAEAKKLLFTSHSPKYSIINAD
EECCCCEEECCCEEEECCHHHHHCCCHHHCCCCCCHHHHHHHHHHEEECCCCCEEEECCC
DTVGASWLKELPNAVGVSLNEGNLSFHSGKKVFATSVIYSTDGIQISLNSDWGTADLSVP
CCCCHHHHHHCCHHHCCEECCCCEEEECCCEEEEEEEEEECCCEEEEECCCCCCCEECEE
LIGAFNANNILVAFATLLSLGYDITELADAAPKLTAVIGRMELFSSEGNAKVVVDYAHTP
EEEECCCCCHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHHHHHCCCCCEEEEEEECCCC
DALDKALQALRVHCEGKLWCIFGCGGDRDTGKRPMMASIAEQQADVIILSDDNPRSEDPS
HHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCHHHHHHHCCCCEEEECCCCCCCCCCC
LITKDMLAGLNQPDIAHVIHQRFDACQFALSQASKHDIILLAGKGHEEYQVLATETIHYS
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCEEEEEEEEEECC
DRETAQKLLGLSS
CHHHHHHHHCCCC
>Mature Secondary Structure
MMQLSELLLPWCELNQVVEISSMTLDSRKVTTGCLFVAIKGHAVDGRQFIPVAIKQGASA
CCCHHHHHCCHHHHCCEEEEHHEEECCCEEEEEEEEEEEECCCCCCCEEEEHEECCCCCE
VLAQSDEVNKTGTISFLDQTPIIYLDALSIKLSAVAGRFYHAEQRKMQLVGITGTNGKTT
EEECCCCCCCCEEEEEECCCCEEEEEEEEEEEEHHHHHHHCCCCCEEEEEEEECCCCCHH
ISQIIAQWATLLGKPAGVMGTTGNGLLTDLKPALNTTGSPIEIQQTLTDLENIGAKLTAL
HHHHHHHHHHHHCCCCCCEECCCCCCHHCCCHHHCCCCCCEEHHHHHHHHHHCCCEEEEE
EISSHGLVQGRVNALNFDAAVFTNLSRDHLDYHGDMANYAEAKKLLFTSHSPKYSIINAD
EECCCCEEECCCEEEECCHHHHHCCCHHHCCCCCCHHHHHHHHHHEEECCCCCEEEECCC
DTVGASWLKELPNAVGVSLNEGNLSFHSGKKVFATSVIYSTDGIQISLNSDWGTADLSVP
CCCCHHHHHHCCHHHCCEECCCCEEEECCCEEEEEEEEEECCCEEEEECCCCCCCEECEE
LIGAFNANNILVAFATLLSLGYDITELADAAPKLTAVIGRMELFSSEGNAKVVVDYAHTP
EEEECCCCCHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHHHHHCCCCCEEEEEEECCCC
DALDKALQALRVHCEGKLWCIFGCGGDRDTGKRPMMASIAEQQADVIILSDDNPRSEDPS
HHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCHHHHHHHCCCCEEEECCCCCCCCCCC
LITKDMLAGLNQPDIAHVIHQRFDACQFALSQASKHDIILLAGKGHEEYQVLATETIHYS
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCEEEEEEEEEECC
DRETAQKLLGLSS
CHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]