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The map label for this gene is murD

Identifier: 209696054

GI number: 209696054

Start: 2863516

End: 2864844

Strand: Reverse

Name: murD

Synonym: VSAL_I2646

Alternate gene names: 209696054

Gene position: 2864844-2863516 (Counterclockwise)

Preceding gene: 209696055

Following gene: 209696053

Centisome position: 86.16

GC content: 40.86

Gene sequence:

>1329_bases
ATGACTGGTTTTGGTGGTGTAAAAAACGTTGTTGTGGTTGGCTTAGGAATGACAGGGTTATCTGTTGTTAAGCATTTGCT
GAGACAACCAGAAGCGTTAATAGTCAAAGTTATTGATACTCGTGAAACGCCACCAGGCCAAGAACAATTGCCTGAACAGG
TTGAGTTACATTCTGGTAGTTGGCAACAAGATTGGCTGTTAGACGCCGATCTTATTGTTACTAACCCAGGTATTGCGCTT
GCTTCTTCTCAATTACAACCAGCCATTAAAAAGGGCACGCCAGTTGTTGGTGATATTGAATTGTTTGCGTGGGCAGTGAC
GGCCCCTGTGATTGCAATTACCGGCTCTAATGGCAAAAGTACGGTCACCGATTTAACCGGTGAAATGGCCAATGCTGCAG
GGGTTAAAACCGCTGTCGGTGGTAATATCGGCTTTGCTGCGTTAGATCTATTAGAACAAGATGCCGATCTTTATGTACTG
GAACTATCCAGTTTTCAACTCGAAACTACATCATCATTAAAGCTAAAAGCCGCCGCATTTTTGAATCTATCTGAAGACCA
TATGGATAGATACCAAGGTATGGAAGATTATCGTCAAGCTAAGTTGCGTGTATTTGATCATGCCGATGTTTGTATTGTTA
ATCGTGATGATAAAGAAACTTACCCAGATACAGCAGATAAAACGTTAATTAGTTTTGGGTTTGATACCAGTGATGAGTAC
GGTTGCATTGAAGAAAATGGTCGGCTGTTTTTAGCTAAAAATAACACACCTATTATTGCTGCCAATGAGCTTGGTCTTGT
TGGTAAACACAATATTGCTAACAGCCTTGTTGCTCTTGCTTTACTTGATGCTGCAGGTGTTAATACCAATAAAACATTAG
ATACGTTGAAGCGTTACAATGGATTAACGCATCGCTGCCAAGTTGTCGCTGAAATGAATGGTGTTCGATGGGTTAACGAT
TCTAAAGCAACTAATGTGGCGAGTACACTGGCTGCACTTTCTGGTCTTAGTATTGAAGGGAAACTTCATTTGTTGGTTGG
TGGTGTTGGTAAAGGGGCTGATTTTTCGGAATTATCGCCAGCATTGAACGAATTGGATTTAATGATGTATTGTTTTGGCG
AGGATGGCGTGCAATTTATGCCTTTAGATCCTCGGTCTCGATTATGCCAAACAATGGATGAGGCGATTGAGTTATTATCC
CCTGAATTAACATTTGGAGATATGGTAATGCTTTCTCCTGCATGTGCCAGTTTTGATCAATATACTAACTTTATGGCAAG
AGGCGATGCATTTACAGCATTAGCTAAACACTATTCAACTGAGAAATAA

Upstream 100 bases:

>100_bases
GAATTGAAAGGGTGGCCAGAACCGCGTGTAATCGTGCGTTTTTGGATTATCTCTATGTTATTGGTTCTTATTGCATTAGC
GACATTGAAGGTACGTTAAT

Downstream 100 bases:

>100_bases
TTTTGGTTATTGAGCGAATTAAGCACTTAGCTTCTCCATTACAAGATTGGGTATTTACCCCATCACCTAAGGTTATGTTT
GATCGTCAATTGATCTGGAT

Product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Number of amino acids: Translated: 442; Mature: 441

Protein sequence:

>442_residues
MTGFGGVKNVVVVGLGMTGLSVVKHLLRQPEALIVKVIDTRETPPGQEQLPEQVELHSGSWQQDWLLDADLIVTNPGIAL
ASSQLQPAIKKGTPVVGDIELFAWAVTAPVIAITGSNGKSTVTDLTGEMANAAGVKTAVGGNIGFAALDLLEQDADLYVL
ELSSFQLETTSSLKLKAAAFLNLSEDHMDRYQGMEDYRQAKLRVFDHADVCIVNRDDKETYPDTADKTLISFGFDTSDEY
GCIEENGRLFLAKNNTPIIAANELGLVGKHNIANSLVALALLDAAGVNTNKTLDTLKRYNGLTHRCQVVAEMNGVRWVND
SKATNVASTLAALSGLSIEGKLHLLVGGVGKGADFSELSPALNELDLMMYCFGEDGVQFMPLDPRSRLCQTMDEAIELLS
PELTFGDMVMLSPACASFDQYTNFMARGDAFTALAKHYSTEK

Sequences:

>Translated_442_residues
MTGFGGVKNVVVVGLGMTGLSVVKHLLRQPEALIVKVIDTRETPPGQEQLPEQVELHSGSWQQDWLLDADLIVTNPGIAL
ASSQLQPAIKKGTPVVGDIELFAWAVTAPVIAITGSNGKSTVTDLTGEMANAAGVKTAVGGNIGFAALDLLEQDADLYVL
ELSSFQLETTSSLKLKAAAFLNLSEDHMDRYQGMEDYRQAKLRVFDHADVCIVNRDDKETYPDTADKTLISFGFDTSDEY
GCIEENGRLFLAKNNTPIIAANELGLVGKHNIANSLVALALLDAAGVNTNKTLDTLKRYNGLTHRCQVVAEMNGVRWVND
SKATNVASTLAALSGLSIEGKLHLLVGGVGKGADFSELSPALNELDLMMYCFGEDGVQFMPLDPRSRLCQTMDEAIELLS
PELTFGDMVMLSPACASFDQYTNFMARGDAFTALAKHYSTEK
>Mature_441_residues
TGFGGVKNVVVVGLGMTGLSVVKHLLRQPEALIVKVIDTRETPPGQEQLPEQVELHSGSWQQDWLLDADLIVTNPGIALA
SSQLQPAIKKGTPVVGDIELFAWAVTAPVIAITGSNGKSTVTDLTGEMANAAGVKTAVGGNIGFAALDLLEQDADLYVLE
LSSFQLETTSSLKLKAAAFLNLSEDHMDRYQGMEDYRQAKLRVFDHADVCIVNRDDKETYPDTADKTLISFGFDTSDEYG
CIEENGRLFLAKNNTPIIAANELGLVGKHNIANSLVALALLDAAGVNTNKTLDTLKRYNGLTHRCQVVAEMNGVRWVNDS
KATNVASTLAALSGLSIEGKLHLLVGGVGKGADFSELSPALNELDLMMYCFGEDGVQFMPLDPRSRLCQTMDEAIELLSP
ELTFGDMVMLSPACASFDQYTNFMARGDAFTALAKHYSTEK

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA)

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family

Homologues:

Organism=Escherichia coli, GI1786276, Length=432, Percent_Identity=54.1666666666667, Blast_Score=427, Evalue=1e-121,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURD_ALISL (B6ELH7)

Other databases:

- EMBL:   FM178379
- RefSeq:   YP_002263984.1
- GeneID:   6988572
- GenomeReviews:   FM178379_GR
- KEGG:   vsa:VSAL_I2646
- HOGENOM:   HBG750024
- OMA:   VKYYNDS
- ProtClustDB:   PRK04663
- GO:   GO:0005737
- HAMAP:   MF_00639
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR01087

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.9

Molecular weight: Translated: 47612; Mature: 47480

Theoretical pI: Translated: 4.45; Mature: 4.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGFGGVKNVVVVGLGMTGLSVVKHLLRQPEALIVKVIDTRETPPGQEQLPEQVELHSGS
CCCCCCCCEEEEEECCCHHHHHHHHHHCCCCEEEEEEEECCCCCCCHHHCCHHHHHCCCC
WQQDWLLDADLIVTNPGIALASSQLQPAIKKGTPVVGDIELFAWAVTAPVIAITGSNGKS
CCCCEEECCCEEEECCCCEEECHHCCHHHHCCCCCEECHHHHHHEECCCEEEEECCCCCC
TVTDLTGEMANAAGVKTAVGGNIGFAALDLLEQDADLYVLELSSFQLETTSSLKLKAAAF
HHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHCCCCEEEEEECCEEEECCCCCEEEEEEE
LNLSEDHMDRYQGMEDYRQAKLRVFDHADVCIVNRDDKETYPDTADKTLISFGFDTSDEY
ECCCHHHHHHHCCHHHHHHHHEEEECCCCEEEEECCCCCCCCCCCCCEEEEECCCCCCCC
GCIEENGRLFLAKNNTPIIAANELGLVGKHNIANSLVALALLDAAGVNTNKTLDTLKRYN
CEEECCCEEEEEECCCCEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHC
GLTHRCQVVAEMNGVRWVNDSKATNVASTLAALSGLSIEGKLHLLVGGVGKGADFSELSP
CCCHHHHHEECCCCEEEECCCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCHHHHHH
ALNELDLMMYCFGEDGVQFMPLDPRSRLCQTMDEAIELLSPELTFGDMVMLSPACASFDQ
HHHHHEEEEEEECCCCCEEEECCHHHHHHHHHHHHHHHHCCCCCCCCEEEECHHHHHHHH
YTNFMARGDAFTALAKHYSTEK
HHHHHHCCHHHHHHHHHHCCCC
>Mature Secondary Structure 
TGFGGVKNVVVVGLGMTGLSVVKHLLRQPEALIVKVIDTRETPPGQEQLPEQVELHSGS
CCCCCCCEEEEEECCCHHHHHHHHHHCCCCEEEEEEEECCCCCCCHHHCCHHHHHCCCC
WQQDWLLDADLIVTNPGIALASSQLQPAIKKGTPVVGDIELFAWAVTAPVIAITGSNGKS
CCCCEEECCCEEEECCCCEEECHHCCHHHHCCCCCEECHHHHHHEECCCEEEEECCCCCC
TVTDLTGEMANAAGVKTAVGGNIGFAALDLLEQDADLYVLELSSFQLETTSSLKLKAAAF
HHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHCCCCEEEEEECCEEEECCCCCEEEEEEE
LNLSEDHMDRYQGMEDYRQAKLRVFDHADVCIVNRDDKETYPDTADKTLISFGFDTSDEY
ECCCHHHHHHHCCHHHHHHHHEEEECCCCEEEEECCCCCCCCCCCCCEEEEECCCCCCCC
GCIEENGRLFLAKNNTPIIAANELGLVGKHNIANSLVALALLDAAGVNTNKTLDTLKRYN
CEEECCCEEEEEECCCCEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHC
GLTHRCQVVAEMNGVRWVNDSKATNVASTLAALSGLSIEGKLHLLVGGVGKGADFSELSP
CCCHHHHHEECCCCEEEECCCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCHHHHHH
ALNELDLMMYCFGEDGVQFMPLDPRSRLCQTMDEAIELLSPELTFGDMVMLSPACASFDQ
HHHHHEEEEEEECCCCCEEEECCHHHHHHHHHHHHHHHHCCCCCCCCEEEECHHHHHHHH
YTNFMARGDAFTALAKHYSTEK
HHHHHHCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA