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The map label for this gene is lpdA [H]

Identifier: 209696033

GI number: 209696033

Start: 2836169

End: 2837596

Strand: Reverse

Name: lpdA [H]

Synonym: VSAL_I2623

Alternate gene names: 209696033

Gene position: 2837596-2836169 (Counterclockwise)

Preceding gene: 209696034

Following gene: 209696032

Centisome position: 85.34

GC content: 42.23

Gene sequence:

>1428_bases
ATGAGCAAAGAAATTAAAGCACAAGTTGTCGTACTTGGTGCCGGCCCTGCTGGTTACTCTGCCGCATTCCGTTGTGCAGA
TTTAGGTTTAGAAACAGTTATCGTTGAACGTTACAACACGCTTGGTGGTGTATGTTTGAATGTGGGTTGTATCCCATCAA
AAGCCTTGCTTCACGTAGCTAAAGTTATCGAAGAAGCAAAAGCGATGGCCGCTCACGGTATCGTATTTGGTGAACCTCAA
ACTGACATCGATAAGATCCGTTTGTGGAAAGAAAAAGTAGTAACACAACTAACTGGCGGTCTTGGCGGTATGGCCAAGAT
GCGTAAAGTAACGGTAGTTAATGGCTTTGGTAAATTTACTGGTGCTAATACTATTGAAGTGACTGCTGAAGATGGCAATA
CAACAATCAATTTTGATAATGCGATTGTTGCTGCTGGTTCTCGTCCAATTAAACTGCCATTCATCCCACATGAAGATCCA
CGTATTTGGGATTCAACGGATGCACTTGAGCTAAAAGAAGTCCCTAAGAAGCTGCTTATTATGGGCGGTGGTATTATCGG
TCTAGAAATGGGTACGGTTTACCATGCTCTGGGTTCTCAAGTTGATGTTGTTGAGATGTTTGACCAGGTTATTCCTGCGG
CAGATAAAGATATCGTTAAAGTTTACACTAAACGTATCAAAGACAAATTCAATCTAATGCTTGAAACGAAAGTGACAGCA
GTAGAAGCAAAAGAAGACGGTATTTACGTATCAATGGAAGGCAAAAAAGCACCAGCAGAAGCTGAGCGTTATGATGCTGT
TCTTGTTGCTATCGGCCGTGTTCCTAATGGTGCTTTATTAGACGTTGAAAAAGCAGGAGTTGCTGTTGATGAGCGTGGTT
TCATCAATGTAGATAAGCAAATGCGTACTAATGTACCTCATATTCATGCCATTGGTGATATCGTAGGTCAACCTATGCTT
GCACACAAAGGCGTGCATGAAGGTCATGTTGCTGCGGAAGTTATTTCTGGTAAGAAGCACTACTTCGATCCTAAAGTAAT
TCCTTCAATTGCGTACACGGAGCCCGAAGTTGCTTGGGTTGGTAAAACAGAGAAAGAAGCAAAAGCTGAAGGCATCAACT
ACGAAGCTGCAAGCTTCCCTTGGGCCGCATCAGGTCGTGCAATCGCTTCAGATTGTGCAGATGGTCTTACTAAGCTTCTT
TTTGATAAAGATACTAACCGTGTTATCGGTGGTGCTATTGTTGGTACTAACGCTGGTGAGCTTCTTGGTGAAATCGGTCT
TGCAATTGAAATGGGTTGTGATGCTGAAGATATCGCATTAACTATCCATGCTCACCCAACATTGCATGAATCAATTGGTA
TGGCTGCTGAAATTTATGAAGGTTCTATTACTGACCTTCCAAATGCAAAAGCCGTTAAGAAAAAATAA

Upstream 100 bases:

>100_bases
TGCTTTCACACTGTTAACATCTATGTAAAATAAAGTCAGTCTGAAAACAATAATAGAATATCGGTCAGCCTGTTAGGGAA
ATGACTATAACGAGGTCAAA

Downstream 100 bases:

>100_bases
TTTTCTTTTAAACCAATAGAATTTAAATATAAAAAAACCGCTGACTTATCAGCGGTTTTTTATTATCTTCTGAATCTACT
TAGTAAGCGTGCGGGGAACT

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 475; Mature: 474

Protein sequence:

>475_residues
MSKEIKAQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGVCLNVGCIPSKALLHVAKVIEEAKAMAAHGIVFGEPQ
TDIDKIRLWKEKVVTQLTGGLGGMAKMRKVTVVNGFGKFTGANTIEVTAEDGNTTINFDNAIVAAGSRPIKLPFIPHEDP
RIWDSTDALELKEVPKKLLIMGGGIIGLEMGTVYHALGSQVDVVEMFDQVIPAADKDIVKVYTKRIKDKFNLMLETKVTA
VEAKEDGIYVSMEGKKAPAEAERYDAVLVAIGRVPNGALLDVEKAGVAVDERGFINVDKQMRTNVPHIHAIGDIVGQPML
AHKGVHEGHVAAEVISGKKHYFDPKVIPSIAYTEPEVAWVGKTEKEAKAEGINYEAASFPWAASGRAIASDCADGLTKLL
FDKDTNRVIGGAIVGTNAGELLGEIGLAIEMGCDAEDIALTIHAHPTLHESIGMAAEIYEGSITDLPNAKAVKKK

Sequences:

>Translated_475_residues
MSKEIKAQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGVCLNVGCIPSKALLHVAKVIEEAKAMAAHGIVFGEPQ
TDIDKIRLWKEKVVTQLTGGLGGMAKMRKVTVVNGFGKFTGANTIEVTAEDGNTTINFDNAIVAAGSRPIKLPFIPHEDP
RIWDSTDALELKEVPKKLLIMGGGIIGLEMGTVYHALGSQVDVVEMFDQVIPAADKDIVKVYTKRIKDKFNLMLETKVTA
VEAKEDGIYVSMEGKKAPAEAERYDAVLVAIGRVPNGALLDVEKAGVAVDERGFINVDKQMRTNVPHIHAIGDIVGQPML
AHKGVHEGHVAAEVISGKKHYFDPKVIPSIAYTEPEVAWVGKTEKEAKAEGINYEAASFPWAASGRAIASDCADGLTKLL
FDKDTNRVIGGAIVGTNAGELLGEIGLAIEMGCDAEDIALTIHAHPTLHESIGMAAEIYEGSITDLPNAKAVKKK
>Mature_474_residues
SKEIKAQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGVCLNVGCIPSKALLHVAKVIEEAKAMAAHGIVFGEPQT
DIDKIRLWKEKVVTQLTGGLGGMAKMRKVTVVNGFGKFTGANTIEVTAEDGNTTINFDNAIVAAGSRPIKLPFIPHEDPR
IWDSTDALELKEVPKKLLIMGGGIIGLEMGTVYHALGSQVDVVEMFDQVIPAADKDIVKVYTKRIKDKFNLMLETKVTAV
EAKEDGIYVSMEGKKAPAEAERYDAVLVAIGRVPNGALLDVEKAGVAVDERGFINVDKQMRTNVPHIHAIGDIVGQPMLA
HKGVHEGHVAAEVISGKKHYFDPKVIPSIAYTEPEVAWVGKTEKEAKAEGINYEAASFPWAASGRAIASDCADGLTKLLF
DKDTNRVIGGAIVGTNAGELLGEIGLAIEMGCDAEDIALTIHAHPTLHESIGMAAEIYEGSITDLPNAKAVKKK

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransfer

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=457, Percent_Identity=43.7636761487965, Blast_Score=350, Evalue=2e-96,
Organism=Homo sapiens, GI50301238, Length=450, Percent_Identity=27.3333333333333, Blast_Score=142, Evalue=6e-34,
Organism=Homo sapiens, GI33519430, Length=427, Percent_Identity=28.1030444964871, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI33519428, Length=427, Percent_Identity=28.1030444964871, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI33519426, Length=427, Percent_Identity=28.1030444964871, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI148277065, Length=428, Percent_Identity=28.0373831775701, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI148277071, Length=428, Percent_Identity=28.0373831775701, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI22035672, Length=428, Percent_Identity=28.5046728971963, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI291045266, Length=452, Percent_Identity=27.6548672566372, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI291045268, Length=443, Percent_Identity=26.6365688487585, Blast_Score=100, Evalue=4e-21,
Organism=Escherichia coli, GI1786307, Length=474, Percent_Identity=87.7637130801688, Blast_Score=857, Evalue=0.0,
Organism=Escherichia coli, GI87082354, Length=467, Percent_Identity=28.2655246252677, Blast_Score=188, Evalue=7e-49,
Organism=Escherichia coli, GI87081717, Length=455, Percent_Identity=28.7912087912088, Blast_Score=184, Evalue=1e-47,
Organism=Escherichia coli, GI1789915, Length=446, Percent_Identity=27.3542600896861, Blast_Score=136, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI32565766, Length=451, Percent_Identity=41.2416851441242, Blast_Score=328, Evalue=5e-90,
Organism=Caenorhabditis elegans, GI17557007, Length=479, Percent_Identity=28.8100208768267, Blast_Score=139, Evalue=5e-33,
Organism=Caenorhabditis elegans, GI71983429, Length=439, Percent_Identity=25.5125284738041, Blast_Score=115, Evalue=7e-26,
Organism=Caenorhabditis elegans, GI71983419, Length=439, Percent_Identity=25.5125284738041, Blast_Score=114, Evalue=8e-26,
Organism=Caenorhabditis elegans, GI71982272, Length=438, Percent_Identity=26.027397260274, Blast_Score=113, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6321091, Length=457, Percent_Identity=42.4507658643326, Blast_Score=320, Evalue=5e-88,
Organism=Saccharomyces cerevisiae, GI6325240, Length=469, Percent_Identity=27.2921108742004, Blast_Score=172, Evalue=7e-44,
Organism=Saccharomyces cerevisiae, GI6325166, Length=455, Percent_Identity=27.9120879120879, Blast_Score=152, Evalue=2e-37,
Organism=Drosophila melanogaster, GI21358499, Length=453, Percent_Identity=41.7218543046358, Blast_Score=337, Evalue=7e-93,
Organism=Drosophila melanogaster, GI24640549, Length=481, Percent_Identity=28.4823284823285, Blast_Score=123, Evalue=3e-28,
Organism=Drosophila melanogaster, GI24640553, Length=481, Percent_Identity=28.4823284823285, Blast_Score=122, Evalue=5e-28,
Organism=Drosophila melanogaster, GI24640551, Length=481, Percent_Identity=28.4823284823285, Blast_Score=122, Evalue=8e-28,
Organism=Drosophila melanogaster, GI17737741, Length=475, Percent_Identity=25.6842105263158, Blast_Score=107, Evalue=2e-23,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 50664; Mature: 50533

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKEIKAQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGVCLNVGCIPSKALLHVA
CCCCCEEEEEEEECCCCCCHHHHHHHHCCHHEEEEHHHHCCCCEEEEECCCCHHHHHHHH
KVIEEAKAMAAHGIVFGEPQTDIDKIRLWKEKVVTQLTGGLGGMAKMRKVTVVNGFGKFT
HHHHHHHHHHHCCEEECCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHEEEEEEECCCCCC
GANTIEVTAEDGNTTINFDNAIVAAGSRPIKLPFIPHEDPRIWDSTDALELKEVPKKLLI
CCCEEEEEECCCCEEEEECCEEEECCCCCEEECCCCCCCCCCCCCCCCHHHHHCCHHEEE
MGGGIIGLEMGTVYHALGSQVDVVEMFDQVIPAADKDIVKVYTKRIKDKFNLMLETKVTA
ECCCEEEEEHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCEEEEEEEEE
VEAKEDGIYVSMEGKKAPAEAERYDAVLVAIGRVPNGALLDVEKAGVAVDERGFINVDKQ
EEECCCCEEEEECCCCCCCHHHHHCEEEEEEECCCCCCEEEEHHCCEEECCCCCEEECHH
MRTNVPHIHAIGDIVGQPMLAHKGVHEGHVAAEVISGKKHYFDPKVIPSIAYTEPEVAWV
HHCCCCEEEEHHHHHCCCHHHHCCCCCCHHHHHHHCCCCCCCCCCCCCCEECCCCCEEEE
GKTEKEAKAEGINYEAASFPWAASGRAIASDCADGLTKLLFDKDTNRVIGGAIVGTNAGE
CCCCHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCHHH
LLGEIGLAIEMGCDAEDIALTIHAHPTLHESIGMAAEIYEGSITDLPNAKAVKKK
HHHHCCCEEEECCCCCCEEEEEECCCCHHHHHCCEEEECCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SKEIKAQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGVCLNVGCIPSKALLHVA
CCCCEEEEEEEECCCCCCHHHHHHHHCCHHEEEEHHHHCCCCEEEEECCCCHHHHHHHH
KVIEEAKAMAAHGIVFGEPQTDIDKIRLWKEKVVTQLTGGLGGMAKMRKVTVVNGFGKFT
HHHHHHHHHHHCCEEECCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHEEEEEEECCCCCC
GANTIEVTAEDGNTTINFDNAIVAAGSRPIKLPFIPHEDPRIWDSTDALELKEVPKKLLI
CCCEEEEEECCCCEEEEECCEEEECCCCCEEECCCCCCCCCCCCCCCCHHHHHCCHHEEE
MGGGIIGLEMGTVYHALGSQVDVVEMFDQVIPAADKDIVKVYTKRIKDKFNLMLETKVTA
ECCCEEEEEHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCEEEEEEEEE
VEAKEDGIYVSMEGKKAPAEAERYDAVLVAIGRVPNGALLDVEKAGVAVDERGFINVDKQ
EEECCCCEEEEECCCCCCCHHHHHCEEEEEEECCCCCCEEEEHHCCEEECCCCCEEECHH
MRTNVPHIHAIGDIVGQPMLAHKGVHEGHVAAEVISGKKHYFDPKVIPSIAYTEPEVAWV
HHCCCCEEEEHHHHHCCCHHHHCCCCCCHHHHHHHCCCCCCCCCCCCCCEECCCCCEEEE
GKTEKEAKAEGINYEAASFPWAASGRAIASDCADGLTKLLFDKDTNRVIGGAIVGTNAGE
CCCCHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCHHH
LLGEIGLAIEMGCDAEDIALTIHAHPTLHESIGMAAEIYEGSITDLPNAKAVKKK
HHHHCCCEEEECCCCCCEEEEEECCCCHHHHHCCEEEECCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]