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The map label for this gene is mrcB [H]

Identifier: 209696016

GI number: 209696016

Start: 2815769

End: 2818144

Strand: Reverse

Name: mrcB [H]

Synonym: VSAL_I2602

Alternate gene names: 209696016

Gene position: 2818144-2815769 (Counterclockwise)

Preceding gene: 209696017

Following gene: 209696015

Centisome position: 84.75

GC content: 39.77

Gene sequence:

>2376_bases
ATGGTTGAAAAAAAGAAAACACCAGTAAAGGCTGCACCAAAAAAGCCGGTAGCAAAAAAGAAAACGCGAACAAAGCGTAA
AGCGACGGCAAAAAAGAAAATACAGAATAAAAATTCGTTTTGGAAAGCTCTATTTATTCTTGGTTTGAAGTTAGGGTTAG
TCGTACTCGCTGTTTTAGTTATCGCGGGTATTTATTTAGATACTGTGGTTAAAAATCGATTTGATGGTCAATTATTTAAA
TTGCCTACGGTTGTTTATAGTCGAGTATTGTCGTTATCTCCTGGGCAAAATATTAGTTTAAAATCCGTTAAAAGTGAATT
GGATTTTTTAAAATACCAAAAAGTAGGACAACCACAGCGATCTGGTGAATATTCATCATCATCAACAAAAATTGAATTGA
TCCGTCGACCTTTTGAATTCCAACAAGGTCCAGAACCGGATCGTCATGTCATGTTGTATTTCAATTCTAATGGTTTACAA
AAAATAGTTAATTTGAGTGCGAAAAAGCAAATGGGCTTTTTACAAATAGAGCCTCAATTTTTAGGTATGCTAGATGCGGA
TGATGACCAACAACGTTTGTTTTTACGTAGAGAGCAATTTCCAGAAGTTATGGTCGATGCGTTATTGGCAACAGAAGATC
GTGATTTTTATCAACATGATGGCGTTTCGCCTTTAGCCATTGCAAGGGCGATGGTGGTAAATATTAAAGCAGGTAGAACA
GTACAAGGCGGAAGTACCTTGACTCAACAACTTGCTAAAAACTTATTTTTATCAAGTGAGCGAACATTGTGGCGAAAAGT
GAGAGAAGCCTACATCGCACTTATCTTGGATTATCGTTACAGTAAAGACCGTATACTAGAAGCGTATTTAAATGAGGTTT
ATTTAGGGCAAACAGGAGGCGAAGCGGTTCATGGCTTTGGTTTGGCTGCTCAAGTTTATTTTGGACGTCCAATTGAAGAG
TTAAGAATTGATCAACTTGCTATGCTTGTTGGTATGGTGAAAGGACCTTCTTATTATAATCCGATGCGCTTCCCTGAAAG
AGTAAAAGAGCGTCGAGATTTGGTATTACGTTTGATGATGCAAGAAGAGATCTTATCACCTCGTCAATATGAAATGGCAG
CGACTCGTCCTTTGGATGTAAAAAAGAGAGCGACGATATCTACCCGCCAACCTGCGTACTTTCAGCAACTGAAATGGGAA
TTGAAAGAAAAAGCAGGGGAGGCGTATCAAAAAGGCGAAGGACTACGAGTCTTTACTACACTTGACCCATTATCACAGCA
AAAAGCAGAACAAGCGGTAGAACGAACGGTTCCTCAGCTAGAGAAGCGAGCAGGTAAGGGATTAGAGGCTGCAATGGTTG
TGGTGGATCGTCAAAGTGGTGAAATTAGAGCGATGATTGGTGGTAGTCGAACGGGATTTGATGGATTTAATCGAGCGATT
AATGCGAAGCGTCCTATTGGTTCGTTAGTAAAACCGGCTATTTATTTATCTGCCTTAGAAAGTCCTGAGAAGTTTTCATT
GGCGAGTACGCTAGATGACAAACCGATCGAATTAAAAGGAAGCAAAGGAACTACGTGGACTCCGAGAAATTTTGATCGCA
AATTCAGAGGGGAAGTCCCTCTTTATTATGCATTTTCGCGTTCGTTAAATGTGCCAACGGTCAACCTTGGCTTAATGGTT
GGTTTAAATAAAGTAACAGATACCTTGGTCAAGCTTGGTATTGAAGCGAGTGAAATTAATCAGGTTCCTTCAATGTTATT
GGGATCATTAAATTTATCTCCTTATCAAGTGGCTCAAATGTATCAAACATTAGGCAACGGTGGGCGAAAGTCACCTTTAA
CGGCACTTAAAGCCGTTGTGAATAGTGATGGAGAGTTACTTTATGAAAACTTCCCTCGATCTTCTCTGGTTGTCTCTGAA
CAAGCGGTTTGGTTAACTATTTATGGGATGAAGAAAGTCGTTAGTGAAGGAACGGCAAGATTTTTAAACAGTAAATACAA
TTGGGCCACTCTAGCGGGTAAAACAGGTACCAGTAATGACTCTAGAGACAGCTGGTATGCAGGGGTTGATGGAAGAGAAG
TCGCGATCACATGGCTTGGAAGAGATGACAATAAGCCGATGAAGTTGACTGGTTCTAGTGGGGCATTAAGGGTGTACGCA
GATTATCTTAGCTTAAGAACGCCAGAAGCTTATCAATTACCTTGGCCTAAAGAAGTGACAACGGCCAGATTTGACTTAGA
AAGTAATAAAACATTAGAGCCGGATTGTTCTGGAAGCGTTAAGTTACCTATCTGGGATAAGAGTGGACAATATAAAGTTG
AATGTGAGAAGAAAAACAGTCCTGCTAGATGGTTAACTGATATTTTTGGGTTGTAA

Upstream 100 bases:

>100_bases
AAGACATATTTGGCCTGATGATCCTGCATCCCATGCAGCAACAAAGAAAACAAAACGTCATTTTAATTAATTTTTATTCT
CTAATTTAAAAAGAACTCTC

Downstream 100 bases:

>100_bases
GTCGTAGTTAATCGAGATTAATGATGGTTTTCAAGAGATGTAATAATTGCGAATGAAGCCTGTGTTTGATGTTGTATTTT
AAGTGCTATGTTTAATGAAA

Product: penicillin-binding protein transpeptidase

Products: NA

Alternate protein names: PBP-1b; PBP1b; Murein polymerase; Penicillin-insensitive transglycosylase; Peptidoglycan TGase; Peptidoglycan glycosyltransferase; Penicillin-sensitive transpeptidase; DD-transpeptidase [H]

Number of amino acids: Translated: 791; Mature: 791

Protein sequence:

>791_residues
MVEKKKTPVKAAPKKPVAKKKTRTKRKATAKKKIQNKNSFWKALFILGLKLGLVVLAVLVIAGIYLDTVVKNRFDGQLFK
LPTVVYSRVLSLSPGQNISLKSVKSELDFLKYQKVGQPQRSGEYSSSSTKIELIRRPFEFQQGPEPDRHVMLYFNSNGLQ
KIVNLSAKKQMGFLQIEPQFLGMLDADDDQQRLFLRREQFPEVMVDALLATEDRDFYQHDGVSPLAIARAMVVNIKAGRT
VQGGSTLTQQLAKNLFLSSERTLWRKVREAYIALILDYRYSKDRILEAYLNEVYLGQTGGEAVHGFGLAAQVYFGRPIEE
LRIDQLAMLVGMVKGPSYYNPMRFPERVKERRDLVLRLMMQEEILSPRQYEMAATRPLDVKKRATISTRQPAYFQQLKWE
LKEKAGEAYQKGEGLRVFTTLDPLSQQKAEQAVERTVPQLEKRAGKGLEAAMVVVDRQSGEIRAMIGGSRTGFDGFNRAI
NAKRPIGSLVKPAIYLSALESPEKFSLASTLDDKPIELKGSKGTTWTPRNFDRKFRGEVPLYYAFSRSLNVPTVNLGLMV
GLNKVTDTLVKLGIEASEINQVPSMLLGSLNLSPYQVAQMYQTLGNGGRKSPLTALKAVVNSDGELLYENFPRSSLVVSE
QAVWLTIYGMKKVVSEGTARFLNSKYNWATLAGKTGTSNDSRDSWYAGVDGREVAITWLGRDDNKPMKLTGSSGALRVYA
DYLSLRTPEAYQLPWPKEVTTARFDLESNKTLEPDCSGSVKLPIWDKSGQYKVECEKKNSPARWLTDIFGL

Sequences:

>Translated_791_residues
MVEKKKTPVKAAPKKPVAKKKTRTKRKATAKKKIQNKNSFWKALFILGLKLGLVVLAVLVIAGIYLDTVVKNRFDGQLFK
LPTVVYSRVLSLSPGQNISLKSVKSELDFLKYQKVGQPQRSGEYSSSSTKIELIRRPFEFQQGPEPDRHVMLYFNSNGLQ
KIVNLSAKKQMGFLQIEPQFLGMLDADDDQQRLFLRREQFPEVMVDALLATEDRDFYQHDGVSPLAIARAMVVNIKAGRT
VQGGSTLTQQLAKNLFLSSERTLWRKVREAYIALILDYRYSKDRILEAYLNEVYLGQTGGEAVHGFGLAAQVYFGRPIEE
LRIDQLAMLVGMVKGPSYYNPMRFPERVKERRDLVLRLMMQEEILSPRQYEMAATRPLDVKKRATISTRQPAYFQQLKWE
LKEKAGEAYQKGEGLRVFTTLDPLSQQKAEQAVERTVPQLEKRAGKGLEAAMVVVDRQSGEIRAMIGGSRTGFDGFNRAI
NAKRPIGSLVKPAIYLSALESPEKFSLASTLDDKPIELKGSKGTTWTPRNFDRKFRGEVPLYYAFSRSLNVPTVNLGLMV
GLNKVTDTLVKLGIEASEINQVPSMLLGSLNLSPYQVAQMYQTLGNGGRKSPLTALKAVVNSDGELLYENFPRSSLVVSE
QAVWLTIYGMKKVVSEGTARFLNSKYNWATLAGKTGTSNDSRDSWYAGVDGREVAITWLGRDDNKPMKLTGSSGALRVYA
DYLSLRTPEAYQLPWPKEVTTARFDLESNKTLEPDCSGSVKLPIWDKSGQYKVECEKKNSPARWLTDIFGL
>Mature_791_residues
MVEKKKTPVKAAPKKPVAKKKTRTKRKATAKKKIQNKNSFWKALFILGLKLGLVVLAVLVIAGIYLDTVVKNRFDGQLFK
LPTVVYSRVLSLSPGQNISLKSVKSELDFLKYQKVGQPQRSGEYSSSSTKIELIRRPFEFQQGPEPDRHVMLYFNSNGLQ
KIVNLSAKKQMGFLQIEPQFLGMLDADDDQQRLFLRREQFPEVMVDALLATEDRDFYQHDGVSPLAIARAMVVNIKAGRT
VQGGSTLTQQLAKNLFLSSERTLWRKVREAYIALILDYRYSKDRILEAYLNEVYLGQTGGEAVHGFGLAAQVYFGRPIEE
LRIDQLAMLVGMVKGPSYYNPMRFPERVKERRDLVLRLMMQEEILSPRQYEMAATRPLDVKKRATISTRQPAYFQQLKWE
LKEKAGEAYQKGEGLRVFTTLDPLSQQKAEQAVERTVPQLEKRAGKGLEAAMVVVDRQSGEIRAMIGGSRTGFDGFNRAI
NAKRPIGSLVKPAIYLSALESPEKFSLASTLDDKPIELKGSKGTTWTPRNFDRKFRGEVPLYYAFSRSLNVPTVNLGLMV
GLNKVTDTLVKLGIEASEINQVPSMLLGSLNLSPYQVAQMYQTLGNGGRKSPLTALKAVVNSDGELLYENFPRSSLVVSE
QAVWLTIYGMKKVVSEGTARFLNSKYNWATLAGKTGTSNDSRDSWYAGVDGREVAITWLGRDDNKPMKLTGSSGALRVYA
DYLSLRTPEAYQLPWPKEVTTARFDLESNKTLEPDCSGSVKLPIWDKSGQYKVECEKKNSPARWLTDIFGL

Specific function: Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell inner membrane; Single-pass type II membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786343, Length=728, Percent_Identity=47.6648351648352, Blast_Score=689, Evalue=0.0,
Organism=Escherichia coli, GI87082258, Length=259, Percent_Identity=38.6100386100386, Blast_Score=187, Evalue=2e-48,
Organism=Escherichia coli, GI1788867, Length=510, Percent_Identity=29.0196078431373, Blast_Score=172, Evalue=8e-44,
Organism=Escherichia coli, GI1789601, Length=169, Percent_Identity=34.9112426035503, Blast_Score=84, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011813
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: =2.4.1.129 [H]

Molecular weight: Translated: 88922; Mature: 88922

Theoretical pI: Translated: 10.24; Mature: 10.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVEKKKTPVKAAPKKPVAKKKTRTKRKATAKKKIQNKNSFWKALFILGLKLGLVVLAVLV
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
IAGIYLDTVVKNRFDGQLFKLPTVVYSRVLSLSPGQNISLKSVKSELDFLKYQKVGQPQR
HHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCC
SGEYSSSSTKIELIRRPFEFQQGPEPDRHVMLYFNSNGLQKIVNLSAKKQMGFLQIEPQF
CCCCCCCCCHHHHHHCCHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCEEEECHHH
LGMLDADDDQQRLFLRREQFPEVMVDALLATEDRDFYQHDGVSPLAIARAMVVNIKAGRT
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCHHHHHHHHEEEECCCCE
VQGGSTLTQQLAKNLFLSSERTLWRKVREAYIALILDYRYSKDRILEAYLNEVYLGQTGG
ECCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHEEECCCCC
EAVHGFGLAAQVYFGRPIEELRIDQLAMLVGMVKGPSYYNPMRFPERVKERRDLVLRLMM
CHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHH
QEEILSPRQYEMAATRPLDVKKRATISTRQPAYFQQLKWELKEKAGEAYQKGEGLRVFTT
HHHHCCCCHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEE
LDPLSQQKAEQAVERTVPQLEKRAGKGLEAAMVVVDRQSGEIRAMIGGSRTGFDGFNRAI
CCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCEEEEECCCCCCCCHHHHHC
NAKRPIGSLVKPAIYLSALESPEKFSLASTLDDKPIELKGSKGTTWTPRNFDRKFRGEVP
CCCCCHHHHHHHHHHHHHHCCCCCEEHHHHCCCCCEEEECCCCCCCCCCCCCHHHCCCCC
LYYAFSRSLNVPTVNLGLMVGLNKVTDTLVKLGIEASEINQVPSMLLGSLNLSPYQVAQM
EEEEEECCCCCCEEEEHHEECCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHH
YQTLGNGGRKSPLTALKAVVNSDGELLYENFPRSSLVVSEQAVWLTIYGMKKVVSEGTAR
HHHHCCCCCCCHHHHHHHHHCCCCCEEECCCCCCCEEEECCEEEEEEHHHHHHHHHHHHH
FLNSKYNWATLAGKTGTSNDSRDSWYAGVDGREVAITWLGRDDNKPMKLTGSSGALRVYA
HHCCCCCEEEEECCCCCCCCCCCCEEECCCCCEEEEEEECCCCCCCEEEECCCCCEEEEE
DYLSLRTPEAYQLPWPKEVTTARFDLESNKTLEPDCSGSVKLPIWDKSGQYKVECEKKNS
HHHHCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCEEEEEECCCCCEEEEECCCCC
PARWLTDIFGL
CHHHHHHHHCC
>Mature Secondary Structure
MVEKKKTPVKAAPKKPVAKKKTRTKRKATAKKKIQNKNSFWKALFILGLKLGLVVLAVLV
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
IAGIYLDTVVKNRFDGQLFKLPTVVYSRVLSLSPGQNISLKSVKSELDFLKYQKVGQPQR
HHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCC
SGEYSSSSTKIELIRRPFEFQQGPEPDRHVMLYFNSNGLQKIVNLSAKKQMGFLQIEPQF
CCCCCCCCCHHHHHHCCHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCEEEECHHH
LGMLDADDDQQRLFLRREQFPEVMVDALLATEDRDFYQHDGVSPLAIARAMVVNIKAGRT
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCHHHHHHHHEEEECCCCE
VQGGSTLTQQLAKNLFLSSERTLWRKVREAYIALILDYRYSKDRILEAYLNEVYLGQTGG
ECCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHEEECCCCC
EAVHGFGLAAQVYFGRPIEELRIDQLAMLVGMVKGPSYYNPMRFPERVKERRDLVLRLMM
CHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHH
QEEILSPRQYEMAATRPLDVKKRATISTRQPAYFQQLKWELKEKAGEAYQKGEGLRVFTT
HHHHCCCCHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEE
LDPLSQQKAEQAVERTVPQLEKRAGKGLEAAMVVVDRQSGEIRAMIGGSRTGFDGFNRAI
CCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCEEEEECCCCCCCCHHHHHC
NAKRPIGSLVKPAIYLSALESPEKFSLASTLDDKPIELKGSKGTTWTPRNFDRKFRGEVP
CCCCCHHHHHHHHHHHHHHCCCCCEEHHHHCCCCCEEEECCCCCCCCCCCCCHHHCCCCC
LYYAFSRSLNVPTVNLGLMVGLNKVTDTLVKLGIEASEINQVPSMLLGSLNLSPYQVAQM
EEEEEECCCCCCEEEEHHEECCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHH
YQTLGNGGRKSPLTALKAVVNSDGELLYENFPRSSLVVSEQAVWLTIYGMKKVVSEGTAR
HHHHCCCCCCCHHHHHHHHHCCCCCEEECCCCCCCEEEECCEEEEEEHHHHHHHHHHHHH
FLNSKYNWATLAGKTGTSNDSRDSWYAGVDGREVAITWLGRDDNKPMKLTGSSGALRVYA
HHCCCCCEEEEECCCCCCCCCCCCEEECCCCCEEEEEEECCCCCCCEEEECCCCCEEEEE
DYLSLRTPEAYQLPWPKEVTTARFDLESNKTLEPDCSGSVKLPIWDKSGQYKVECEKKNS
HHHHCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCEEEEEECCCCCEEEEECCCCC
PARWLTDIFGL
CHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]