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The map label for this gene is napF [H]

Identifier: 209695793

GI number: 209695793

Start: 2541560

End: 2542078

Strand: Reverse

Name: napF [H]

Synonym: VSAL_I2368

Alternate gene names: 209695793

Gene position: 2542078-2541560 (Counterclockwise)

Preceding gene: 209695797

Following gene: 209695792

Centisome position: 76.45

GC content: 40.85

Gene sequence:

>519_bases
ATGAGTCAAGATAATTCAGTTGACCATTCCAAGCGGGGATTTTTTCGCCGTTTGTCTCCTCAGAAAAAAATACATCCTTT
AACACAGCAGCGTTTGCCTTGGGTTCATAATGAAGCTGAGTTTGTCTCTGCTTGTACTCGCTGTGAAAAGTGCATTACCG
CATGTGAAGAAAGCATAATTGTTAAAGGCGATGGGGGTTTTCCTATCGTTGATTTTACAAAAGGTGAGTGCACTTTTTGT
GAACAATGTGCATCAGTATGCCCTGAACACTTATTTACTGTAACTGAAAAATCAGCTTTCTCTCATCGAATTACAATAAA
TGAAAGTTGTTTAGCAAAACGAGCCGTTGAATGTCGTAGTTGCAGTGATATGTGTGACACGCAAGCGATTCGTTTTCAAC
TTACGCTGGGCAGTGTAGCTCAGCCTAAACTTAATTTTGATGACTGTAATGGATGTGGTGCTTGTGTTGCAGCATGCCCA
ACTTCAGCTATCAGCATGACACTGGAAGAGCAAACATAA

Upstream 100 bases:

>100_bases
TTCCCCCCTTGTTTAACCATTATGAGGTATATCAATAAATATGACAAAATTTAAAATTCATCATATAAAAATGTTAATAG
ATGCGCGGAGTTGTGAATAG

Downstream 100 bases:

>100_bases
TGCTAGAAAATTTGCCTGAGAACGAAGTTCATATTTCAAGTGTAGTCGTACATGTCAAACCTGAATTTTTAGAACAAACA
AAAGCAAAAATATTAGAAAT

Product: ferredoxin-type protein NapF

Products: reduced acceptor; NO3- [C]

Alternate protein names: NA

Number of amino acids: Translated: 172; Mature: 171

Protein sequence:

>172_residues
MSQDNSVDHSKRGFFRRLSPQKKIHPLTQQRLPWVHNEAEFVSACTRCEKCITACEESIIVKGDGGFPIVDFTKGECTFC
EQCASVCPEHLFTVTEKSAFSHRITINESCLAKRAVECRSCSDMCDTQAIRFQLTLGSVAQPKLNFDDCNGCGACVAACP
TSAISMTLEEQT

Sequences:

>Translated_172_residues
MSQDNSVDHSKRGFFRRLSPQKKIHPLTQQRLPWVHNEAEFVSACTRCEKCITACEESIIVKGDGGFPIVDFTKGECTFC
EQCASVCPEHLFTVTEKSAFSHRITINESCLAKRAVECRSCSDMCDTQAIRFQLTLGSVAQPKLNFDDCNGCGACVAACP
TSAISMTLEEQT
>Mature_171_residues
SQDNSVDHSKRGFFRRLSPQKKIHPLTQQRLPWVHNEAEFVSACTRCEKCITACEESIIVKGDGGFPIVDFTKGECTFCE
QCASVCPEHLFTVTEKSAFSHRITINESCLAKRAVECRSCSDMCDTQAIRFQLTLGSVAQPKLNFDDCNGCGACVAACPT
SAISMTLEEQT

Specific function: Involved in electron transfer [H]

COG id: COG1145

COG function: function code C; Ferredoxin

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1788536, Length=161, Percent_Identity=45.3416149068323, Blast_Score=150, Evalue=5e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR004496 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: NA

Molecular weight: Translated: 18997; Mature: 18866

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: PS00198 4FE4S_FERREDOXIN ; PS00242 INTEGRIN_ALPHA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

9.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
11.0 %Cys+Met (Translated Protein)
9.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
10.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQDNSVDHSKRGFFRRLSPQKKIHPLTQQRLPWVHNEAEFVSACTRCEKCITACEESII
CCCCCCCCCHHHHHHHHCCCCHHCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCEE
VKGDGGFPIVDFTKGECTFCEQCASVCPEHLFTVTEKSAFSHRITINESCLAKRAVECRS
EECCCCCEEEECCCCCCHHHHHHHHHCHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHH
CSDMCDTQAIRFQLTLGSVAQPKLNFDDCNGCGACVAACPTSAISMTLEEQT
HHHHHCCCEEEEEEEECCCCCCCCCCCCCCCCHHHHHHCCCHHHEEEECCCC
>Mature Secondary Structure 
SQDNSVDHSKRGFFRRLSPQKKIHPLTQQRLPWVHNEAEFVSACTRCEKCITACEESII
CCCCCCCCHHHHHHHHCCCCHHCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCEE
VKGDGGFPIVDFTKGECTFCEQCASVCPEHLFTVTEKSAFSHRITINESCLAKRAVECRS
EECCCCCEEEECCCCCCHHHHHHHHHCHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHH
CSDMCDTQAIRFQLTLGSVAQPKLNFDDCNGCGACVAACPTSAISMTLEEQT
HHHHHCCCEEEEEEEECCCCCCCCCCCCCCCCHHHHHHCCCHHHEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Acceptor; nitrite [C]

Specific reaction: Acceptor + nitrite = reduced acceptor + NO3- [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]