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The map label for this gene is ptsI [H]

Identifier: 209695784

GI number: 209695784

Start: 2532621

End: 2534345

Strand: Direct

Name: ptsI [H]

Synonym: VSAL_I2358

Alternate gene names: 209695784

Gene position: 2532621-2534345 (Clockwise)

Preceding gene: 209695783

Following gene: 209695785

Centisome position: 76.17

GC content: 40.46

Gene sequence:

>1725_bases
ATGATTTCTGGCATTCTAGCATCTCCGGGTATTGCTTTTGGTAAAGCATTATTACTTCAGGAAGATGAAATTGTCCTAAA
CAAAACTCCAATCTCTGAATCTCAAGTAGAAACAGAAATTCAATGTTTCTTTGACGCTAGAGCTAAGTCTTCTGAGCAAT
TAGAAGCGGTTAAAGCGAAAGCGCTAGCAACATTTGGCGAAGAAAAAGAAGCGATTTTTGAGGGGCATATAATGCTTCTT
GAAGATGAAGAGCTAGAAGAAGAAATCTTAGCCCTTATAAGAGACGACAAACTTTCTGCTGATTTTGCAATTTACTCTGT
AATTGAAGAGCAAGCGTGTGCACTTGAATCTTTAGATGATGACTATCTAAAAGAGCGCGCTACAGATATCCGTGATATCG
GTAGCCGCTTTGTTAAAAATGCACTAGGCATTACAATTGTTTCTTTAAGCAACATTAATGAAGAAGTTATCCTGGTTGCT
AAAGATTTAACACCGTCTGAAACGGCACAAATAAACCTAGATTACGTTCTTGGTTTTGCTTGTGACATTGGCGGACGTAC
TTCTCATACTTCTATCATGGCACGTTCTCTTGAACTTCCTGCTATCGTTGGTACTAACGATATCACTAAGCAAGTGAAAA
ACGGTGACATGTTGATTCTTGATGCGGTTAACAACAGAATTGTTATCAACCCATCAGAAGCAGAACTGGCTAAATCGAAA
AAAATTCGTGACGACTTAGTTGCTGAAAAAGAAGAGCTAGCAAAACTAAAAGACCTACCTGCTATCACTCTAGATGGCCA
CCAAGTTGAAGTTTGCGGTAACATCGGCACAGTAAAAGACTGTGAAGGTATTATTCGTAACGGCGGTGAAGGTGTTGGTC
TGTATCGTACAGAATTCCTATTCATGGATCGTGATGCACTTCCTACTGAAGAAGAGCAATATGTTGCTTATAAAGAAGTA
GCAGAAGCAATGCATGGTGAATCAGTGATTATCCGTACTATGGATATCGGTGGTGATAAAGATCTACCATACATGGATCT
TCCTGAAGAAATGAACCCATTCTTAGGATGGCGTGCAATCCGTATCAGTTTAGACCGTCGTGAAATTCTACGTGACCAAT
TACGTGGTATTTTACGTGCATCAGCACACGGCAAACTGCGTATCATGTTCCCAATGATCATTTCTGTTGAAGAAATCCGT
GAGCTGAAAAAAGCAATCGAAGAATACAAAGTAGAACTTCGAGCTGAAGGCCTAGCGTTTGACGAAAGCATCGAGATCGG
TGTTATGGTTGAAACGCCAGCAGCCGCTGCCATTGCACATCATTTAGCGAAAGAAGTTAGCTTCTTCTCTATCGGTACTA
ACGATTTAACACAATATACTCTTGCTGTTGACCGTGGTAACGAAATGATTTCTCACCTATACAACCCACTATCTCCAGCG
GTTCTGCTTGTAATCAAGCAAGTAATCGATGCTTCTCATAAAGAAGGCAAATGGACAGGTATGTGTGGCGAGTTAGCGGG
TGATGATCGTGCAAGCTTACTTCTTCTTGGTATGGGCTTAGACGAATTCAGCATGAGCGGTATTTCTATCCCTCTTGTTA
AGAAAATCGTTCGTAACTCAAACTTTGCAGCAGTAAAAGCAATGGCAGATGAAGCACTTCAAATGCCTACAGCAGCAGAA
ATTGAAGCACACGTTGAAAAATTTATCGCAGAAAATACTAAGTAA

Upstream 100 bases:

>100_bases
AGCAGTAGACCACCTAGTTAAACTAATGGACGAGCTGCATTAATCAGCTTTTTCTTTAATTATTCTCATTCTCTAATTTG
ATCAATGTTAAGGTAAAGCT

Downstream 100 bases:

>100_bases
TAGTATAGTTGCAAAGAATTAAATATAGCCACTAAGTTTTAGTGGCTATTTAATACAAACTTTAGGGAGCAACACAATGG
GTCTGTTTGACAAAATTAAA

Product: phosphoenolpyruvate-protein phosphotransferase

Products: NA

Alternate protein names: Phosphotransferase system, enzyme I [H]

Number of amino acids: Translated: 574; Mature: 574

Protein sequence:

>574_residues
MISGILASPGIAFGKALLLQEDEIVLNKTPISESQVETEIQCFFDARAKSSEQLEAVKAKALATFGEEKEAIFEGHIMLL
EDEELEEEILALIRDDKLSADFAIYSVIEEQACALESLDDDYLKERATDIRDIGSRFVKNALGITIVSLSNINEEVILVA
KDLTPSETAQINLDYVLGFACDIGGRTSHTSIMARSLELPAIVGTNDITKQVKNGDMLILDAVNNRIVINPSEAELAKSK
KIRDDLVAEKEELAKLKDLPAITLDGHQVEVCGNIGTVKDCEGIIRNGGEGVGLYRTEFLFMDRDALPTEEEQYVAYKEV
AEAMHGESVIIRTMDIGGDKDLPYMDLPEEMNPFLGWRAIRISLDRREILRDQLRGILRASAHGKLRIMFPMIISVEEIR
ELKKAIEEYKVELRAEGLAFDESIEIGVMVETPAAAAIAHHLAKEVSFFSIGTNDLTQYTLAVDRGNEMISHLYNPLSPA
VLLVIKQVIDASHKEGKWTGMCGELAGDDRASLLLLGMGLDEFSMSGISIPLVKKIVRNSNFAAVKAMADEALQMPTAAE
IEAHVEKFIAENTK

Sequences:

>Translated_574_residues
MISGILASPGIAFGKALLLQEDEIVLNKTPISESQVETEIQCFFDARAKSSEQLEAVKAKALATFGEEKEAIFEGHIMLL
EDEELEEEILALIRDDKLSADFAIYSVIEEQACALESLDDDYLKERATDIRDIGSRFVKNALGITIVSLSNINEEVILVA
KDLTPSETAQINLDYVLGFACDIGGRTSHTSIMARSLELPAIVGTNDITKQVKNGDMLILDAVNNRIVINPSEAELAKSK
KIRDDLVAEKEELAKLKDLPAITLDGHQVEVCGNIGTVKDCEGIIRNGGEGVGLYRTEFLFMDRDALPTEEEQYVAYKEV
AEAMHGESVIIRTMDIGGDKDLPYMDLPEEMNPFLGWRAIRISLDRREILRDQLRGILRASAHGKLRIMFPMIISVEEIR
ELKKAIEEYKVELRAEGLAFDESIEIGVMVETPAAAAIAHHLAKEVSFFSIGTNDLTQYTLAVDRGNEMISHLYNPLSPA
VLLVIKQVIDASHKEGKWTGMCGELAGDDRASLLLLGMGLDEFSMSGISIPLVKKIVRNSNFAAVKAMADEALQMPTAAE
IEAHVEKFIAENTK
>Mature_574_residues
MISGILASPGIAFGKALLLQEDEIVLNKTPISESQVETEIQCFFDARAKSSEQLEAVKAKALATFGEEKEAIFEGHIMLL
EDEELEEEILALIRDDKLSADFAIYSVIEEQACALESLDDDYLKERATDIRDIGSRFVKNALGITIVSLSNINEEVILVA
KDLTPSETAQINLDYVLGFACDIGGRTSHTSIMARSLELPAIVGTNDITKQVKNGDMLILDAVNNRIVINPSEAELAKSK
KIRDDLVAEKEELAKLKDLPAITLDGHQVEVCGNIGTVKDCEGIIRNGGEGVGLYRTEFLFMDRDALPTEEEQYVAYKEV
AEAMHGESVIIRTMDIGGDKDLPYMDLPEEMNPFLGWRAIRISLDRREILRDQLRGILRASAHGKLRIMFPMIISVEEIR
ELKKAIEEYKVELRAEGLAFDESIEIGVMVETPAAAAIAHHLAKEVSFFSIGTNDLTQYTLAVDRGNEMISHLYNPLSPA
VLLVIKQVIDASHKEGKWTGMCGELAGDDRASLLLLGMGLDEFSMSGISIPLVKKIVRNSNFAAVKAMADEALQMPTAAE
IEAHVEKFIAENTK

Specific function: General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their tr

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=573, Percent_Identity=73.2984293193717, Blast_Score=840, Evalue=0.0,
Organism=Escherichia coli, GI1788726, Length=577, Percent_Identity=38.1282495667244, Blast_Score=368, Evalue=1e-103,
Organism=Escherichia coli, GI48994992, Length=502, Percent_Identity=38.0478087649402, Blast_Score=332, Evalue=3e-92,
Organism=Escherichia coli, GI1789193, Length=576, Percent_Identity=33.5069444444444, Blast_Score=301, Evalue=8e-83,
Organism=Escherichia coli, GI1787994, Length=388, Percent_Identity=26.2886597938144, Blast_Score=104, Evalue=2e-23,
Organism=Escherichia coli, GI226510935, Length=174, Percent_Identity=29.8850574712644, Blast_Score=75, Evalue=9e-15,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 63267; Mature: 63267

Theoretical pI: Translated: 4.38; Mature: 4.38

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISGILASPGIAFGKALLLQEDEIVLNKTPISESQVETEIQCFFDARAKSSEQLEAVKAK
CCCCCCCCCCHHHHHHHEEECCCEEEECCCCCHHHHHHHHHEEEECCCCCHHHHHHHHHH
ALATFGEEKEAIFEGHIMLLEDEELEEEILALIRDDKLSADFAIYSVIEEQACALESLDD
HHHHCCCCHHHEECCCEEEEECHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCH
DYLKERATDIRDIGSRFVKNALGITIVSLSNINEEVILVAKDLTPSETAQINLDYVLGFA
HHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCEEEEEEECCCCCCCEEEEEEEEEEEE
CDIGGRTSHTSIMARSLELPAIVGTNDITKQVKNGDMLILDAVNNRIVINPSEAELAKSK
EECCCCCCHHHHHHHHCCCCEEECCCHHHHHHCCCCEEEEEECCCEEEECCCHHHHHHHH
KIRDDLVAEKEELAKLKDLPAITLDGHQVEVCGNIGTVKDCEGIIRNGGEGVGLYRTEFL
HHHHHHHHHHHHHHHHHCCCEEEECCCEEEEECCCCCHHHHHHHHHCCCCCCCEEEEEEE
FMDRDALPTEEEQYVAYKEVAEAMHGESVIIRTMDIGGDKDLPYMDLPEEMNPFLGWRAI
EEECCCCCCCHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCHHCCHHHCEEEE
RISLDRREILRDQLRGILRASAHGKLRIMFPMIISVEEIRELKKAIEEYKVELRAEGLAF
EEECCHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
DESIEIGVMVETPAAAAIAHHLAKEVSFFSIGTNDLTQYTLAVDRGNEMISHLYNPLSPA
CCCEEEEEEEECCHHHHHHHHHHHHCEEEEECCCCCEEEEEEEECCHHHHHHHHCCCCHH
VLLVIKQVIDASHKEGKWTGMCGELAGDDRASLLLLGMGLDEFSMSGISIPLVKKIVRNS
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCHHCCCCCCHHHHHHHHCCC
NFAAVKAMADEALQMPTAAEIEAHVEKFIAENTK
CCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MISGILASPGIAFGKALLLQEDEIVLNKTPISESQVETEIQCFFDARAKSSEQLEAVKAK
CCCCCCCCCCHHHHHHHEEECCCEEEECCCCCHHHHHHHHHEEEECCCCCHHHHHHHHHH
ALATFGEEKEAIFEGHIMLLEDEELEEEILALIRDDKLSADFAIYSVIEEQACALESLDD
HHHHCCCCHHHEECCCEEEEECHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCH
DYLKERATDIRDIGSRFVKNALGITIVSLSNINEEVILVAKDLTPSETAQINLDYVLGFA
HHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCEEEEEEECCCCCCCEEEEEEEEEEEE
CDIGGRTSHTSIMARSLELPAIVGTNDITKQVKNGDMLILDAVNNRIVINPSEAELAKSK
EECCCCCCHHHHHHHHCCCCEEECCCHHHHHHCCCCEEEEEECCCEEEECCCHHHHHHHH
KIRDDLVAEKEELAKLKDLPAITLDGHQVEVCGNIGTVKDCEGIIRNGGEGVGLYRTEFL
HHHHHHHHHHHHHHHHHCCCEEEECCCEEEEECCCCCHHHHHHHHHCCCCCCCEEEEEEE
FMDRDALPTEEEQYVAYKEVAEAMHGESVIIRTMDIGGDKDLPYMDLPEEMNPFLGWRAI
EEECCCCCCCHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCHHCCHHHCEEEE
RISLDRREILRDQLRGILRASAHGKLRIMFPMIISVEEIRELKKAIEEYKVELRAEGLAF
EEECCHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
DESIEIGVMVETPAAAAIAHHLAKEVSFFSIGTNDLTQYTLAVDRGNEMISHLYNPLSPA
CCCEEEEEEEECCHHHHHHHHHHHHCEEEEECCCCCEEEEEEEECCHHHHHHHHCCCCHH
VLLVIKQVIDASHKEGKWTGMCGELAGDDRASLLLLGMGLDEFSMSGISIPLVKKIVRNS
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCHHCCCCCCHHHHHHHHCCC
NFAAVKAMADEALQMPTAAEIEAHVEKFIAENTK
CCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2457575; 2960675; 9205837; 9278503; 2411636; 3290198; 9298646; 7876255; 8805571; 9054557; 9541412; 10048929 [H]