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The map label for this gene is pflB [H]

Identifier: 209695415

GI number: 209695415

Start: 2094014

End: 2096290

Strand: Reverse

Name: pflB [H]

Synonym: VSAL_I1955

Alternate gene names: 209695415

Gene position: 2096290-2094014 (Counterclockwise)

Preceding gene: 209695416

Following gene: 209695414

Centisome position: 63.04

GC content: 43.35

Gene sequence:

>2277_bases
ATGGCAGAGCAATTTGCTAAAGCTTGGACAGGTTTTGCAGACGGTGAGTGGCAAAACGAAGTTAACGTTCGCGATTTCAT
CCAAAAGAACTATGCACCTTATGAAGGTGATGAAGAATTCTTAGTTTCTGAAGGTACTGAAGCAACTAACAAGCTTTGGG
CTAAAGTAATGGAAGGCATCAAGCAGGAAAACGCAACTCATGCGCCTGTTGATTTTGATACTTCTCTTATCTCTACCATC
ACATCTCACGATGCGGGCTACATCAACCAAGACCTAGAAACAATCGTTGGTCTTCAAACTGATGCTCCTCTAAAACGTGC
AATCATCCCTAATGGTGGTGTACGTATGGTAGAAGGTTCTTGTAAAGCATACGATCGCGTTCTTGATCCTATGGTTTCAA
AAATCTATTCTGAATACCGTAAAACACACAATGCTGGTGTTTTCGATATCTACACTAAAGACATTTTAAACTGTCGTAAA
TCAGGTGTTCTAACAGGTCTTCCTGATGCTTACGGCCGTGGTCGTATCATTGGTGATTACCGTCGTGTTGCACTTTACGG
TATTGATTTCCTTATCACAGACAAACTGGCTCAATTCGCTTCTTTACAAGAACGTTTTGAAAAAGGCGAAGACCTAACAG
CAACAATGCAACTTCGTGAAGAAATTGCTGAGCAACACCGCGCTCTAGGTCAAATCAAAGTGATGGCAGCTAAATACGGC
TGTGATATTTCAGGTCCTGCTACAACTGCTCAAGAAGCAATTCAGTGGACTTACTTCGGTTACCTAGCTGCTGTTAAATC
TCAAAACGGTGCTGCAATGTCTCTAGGTCGTACTTCGACTTTCCTAGACATATACATCCAACGTGATATCGAAGCAGGTC
TTCTAACTGAAGAGCTAGCTCAAGAAATGATCGATCACTTCGTAATGAAGCTACGTATGGTTCGTTTCCTACGTACTCCT
GAATACGATGAATTGTTCTCTGGTGACCCAATCTGGGCAACAGAATCTATGGGTGGTATGGGTCTTGACGGTCGTACACT
AGTTACACGTACTAACTTCCGTTTCCTAAACAGCCTATACACAATGGGTCCTTCTCCAGAGCCAAACATCACTGTTCTTT
GGTCTGAGCAACTGCCTGTAGGCTTCAAACGTTTCTGTGCGAAGGTATCTATCGATACATCTTCTATCCAGTACGAAAAT
GATGACCTAATGCGTCCTGACCTAGGTTCTGATGATTACGCAATCGCTTGTTGTGTATCTCCAATGATCGTTGGTAAGCA
AATGCAGTTCTTCGGTGCTCGTGCTAACCTTGCGAAAACAATGCTTTACTCTATCAACGGCGGCGTTGATGAGAAACTGA
AAATCCAAATTGGTCCTAAAGAAGCACCAATGACAGATGAAGTTCTTGATTACGCTAAAGTAATGGATCGCCTAGATCAC
TTCATGGATTGGTTAGCAACTCAATACGTTACAGCATTGAACTCTATCCACTACATGCACGACAAATACAGCTACGAAGC
GTCTCTAATGGCTCTTCATGACCGTGACGTTCGTCGTACAATGGCGTGTGGTATTGCTGGTCTATCTGTTGCAGCCGATT
CACTATCTGCAATCAAATACGCGACAGTTAAACCAATTCGTGACGAAAACGGCATTGCTATCGATTTCGACATCGAAGGT
GACTACCCTAAATTTGGTAACAATGACGCTCGCGTTGATGATATCGCATGTGAACTAGTTTCTACGTTCATGGGTAAAAT
CCGTAAGCTTACTATGTACCGTAATGCTATCCCTACTCAATCAATCCTTACTATTACATCTAACGTTGTATACGGTAAGA
AAACGGGTAACACACCAGATGGTCGTCAAGCAGGTGCTCCTTTTGCTCCTGGTGCTAACCCAATGCACGGCCGTGATGAG
AAAGGTGCAGTAGCATCACTTACATCAGTAGGTAAACTACCGTTTGCTGATGCTCAAGATGGTATCTCTTACACATTCTC
TATCGTACCTAACGCACTAGGTAAAGACGATGACAACCGTCGTTCTAACCTTGCTGGCCTAATGGATGGTTACTTCCACC
ATGAAGCTAACGTAGAAGGTGGTCAACACCTTAACGTTAACGTTCTTAACCGTGAAACTCTAGAAGACGCAGTTAAGCAC
CCAGAGAACTACCCACAACTGACTATCCGTGTATCGGGTTATGCTGTGCGTTTTAACTCTCTAACGACAGAGCAACAAGC
TGATGTAATCGCACGTACTTTCACTGCGACTCTATAA

Upstream 100 bases:

>100_bases
ATTAAATATAGAGTGTACTATTTGCCCATACGGGTTGTGGTCTATTCATAACACCACTGAAAATAGTACGTATTTTTTCT
ACTAAAAAGGTAGGTATATC

Downstream 100 bases:

>100_bases
TTCAGCACTAAGTAAGTCATATTTAAGCCCTGCCATTTGGTGTAATGCCGATCATTTAAGGTAAATATGATCGGTTGACC
GATCCTTTTTAGATGTCAAA

Product: formate acetyltransferase 1

Products: NA

Alternate protein names: Pyruvate formate-lyase 1 [H]

Number of amino acids: Translated: 758; Mature: 757

Protein sequence:

>758_residues
MAEQFAKAWTGFADGEWQNEVNVRDFIQKNYAPYEGDEEFLVSEGTEATNKLWAKVMEGIKQENATHAPVDFDTSLISTI
TSHDAGYINQDLETIVGLQTDAPLKRAIIPNGGVRMVEGSCKAYDRVLDPMVSKIYSEYRKTHNAGVFDIYTKDILNCRK
SGVLTGLPDAYGRGRIIGDYRRVALYGIDFLITDKLAQFASLQERFEKGEDLTATMQLREEIAEQHRALGQIKVMAAKYG
CDISGPATTAQEAIQWTYFGYLAAVKSQNGAAMSLGRTSTFLDIYIQRDIEAGLLTEELAQEMIDHFVMKLRMVRFLRTP
EYDELFSGDPIWATESMGGMGLDGRTLVTRTNFRFLNSLYTMGPSPEPNITVLWSEQLPVGFKRFCAKVSIDTSSIQYEN
DDLMRPDLGSDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYSINGGVDEKLKIQIGPKEAPMTDEVLDYAKVMDRLDH
FMDWLATQYVTALNSIHYMHDKYSYEASLMALHDRDVRRTMACGIAGLSVAADSLSAIKYATVKPIRDENGIAIDFDIEG
DYPKFGNNDARVDDIACELVSTFMGKIRKLTMYRNAIPTQSILTITSNVVYGKKTGNTPDGRQAGAPFAPGANPMHGRDE
KGAVASLTSVGKLPFADAQDGISYTFSIVPNALGKDDDNRRSNLAGLMDGYFHHEANVEGGQHLNVNVLNRETLEDAVKH
PENYPQLTIRVSGYAVRFNSLTTEQQADVIARTFTATL

Sequences:

>Translated_758_residues
MAEQFAKAWTGFADGEWQNEVNVRDFIQKNYAPYEGDEEFLVSEGTEATNKLWAKVMEGIKQENATHAPVDFDTSLISTI
TSHDAGYINQDLETIVGLQTDAPLKRAIIPNGGVRMVEGSCKAYDRVLDPMVSKIYSEYRKTHNAGVFDIYTKDILNCRK
SGVLTGLPDAYGRGRIIGDYRRVALYGIDFLITDKLAQFASLQERFEKGEDLTATMQLREEIAEQHRALGQIKVMAAKYG
CDISGPATTAQEAIQWTYFGYLAAVKSQNGAAMSLGRTSTFLDIYIQRDIEAGLLTEELAQEMIDHFVMKLRMVRFLRTP
EYDELFSGDPIWATESMGGMGLDGRTLVTRTNFRFLNSLYTMGPSPEPNITVLWSEQLPVGFKRFCAKVSIDTSSIQYEN
DDLMRPDLGSDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYSINGGVDEKLKIQIGPKEAPMTDEVLDYAKVMDRLDH
FMDWLATQYVTALNSIHYMHDKYSYEASLMALHDRDVRRTMACGIAGLSVAADSLSAIKYATVKPIRDENGIAIDFDIEG
DYPKFGNNDARVDDIACELVSTFMGKIRKLTMYRNAIPTQSILTITSNVVYGKKTGNTPDGRQAGAPFAPGANPMHGRDE
KGAVASLTSVGKLPFADAQDGISYTFSIVPNALGKDDDNRRSNLAGLMDGYFHHEANVEGGQHLNVNVLNRETLEDAVKH
PENYPQLTIRVSGYAVRFNSLTTEQQADVIARTFTATL
>Mature_757_residues
AEQFAKAWTGFADGEWQNEVNVRDFIQKNYAPYEGDEEFLVSEGTEATNKLWAKVMEGIKQENATHAPVDFDTSLISTIT
SHDAGYINQDLETIVGLQTDAPLKRAIIPNGGVRMVEGSCKAYDRVLDPMVSKIYSEYRKTHNAGVFDIYTKDILNCRKS
GVLTGLPDAYGRGRIIGDYRRVALYGIDFLITDKLAQFASLQERFEKGEDLTATMQLREEIAEQHRALGQIKVMAAKYGC
DISGPATTAQEAIQWTYFGYLAAVKSQNGAAMSLGRTSTFLDIYIQRDIEAGLLTEELAQEMIDHFVMKLRMVRFLRTPE
YDELFSGDPIWATESMGGMGLDGRTLVTRTNFRFLNSLYTMGPSPEPNITVLWSEQLPVGFKRFCAKVSIDTSSIQYEND
DLMRPDLGSDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYSINGGVDEKLKIQIGPKEAPMTDEVLDYAKVMDRLDHF
MDWLATQYVTALNSIHYMHDKYSYEASLMALHDRDVRRTMACGIAGLSVAADSLSAIKYATVKPIRDENGIAIDFDIEGD
YPKFGNNDARVDDIACELVSTFMGKIRKLTMYRNAIPTQSILTITSNVVYGKKTGNTPDGRQAGAPFAPGANPMHGRDEK
GAVASLTSVGKLPFADAQDGISYTFSIVPNALGKDDDNRRSNLAGLMDGYFHHEANVEGGQHLNVNVLNRETLEDAVKHP
ENYPQLTIRVSGYAVRFNSLTTEQQADVIARTFTATL

Specific function: Glucose metabolism (nonoxidative conversion). [C]

COG id: COG1882

COG function: function code C; Pyruvate-formate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pyruvate formate lyase domain [H]

Homologues:

Organism=Escherichia coli, GI1787131, Length=758, Percent_Identity=80.3430079155673, Blast_Score=1309, Evalue=0.0,
Organism=Escherichia coli, GI48994926, Length=754, Percent_Identity=74.6684350132626, Blast_Score=1217, Evalue=0.0,
Organism=Escherichia coli, GI1787044, Length=595, Percent_Identity=28.4033613445378, Blast_Score=182, Evalue=8e-47,
Organism=Escherichia coli, GI1790388, Length=648, Percent_Identity=25.7716049382716, Blast_Score=131, Evalue=1e-31,
Organism=Escherichia coli, GI1788933, Length=64, Percent_Identity=87.5, Blast_Score=117, Evalue=2e-27,

Paralogues:

None

Copy number: 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005949
- InterPro:   IPR001150
- InterPro:   IPR019777
- InterPro:   IPR004184 [H]

Pfam domain/function: PF01228 Gly_radical; PF02901 PFL [H]

EC number: =2.3.1.54 [H]

Molecular weight: Translated: 84200; Mature: 84069

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS00850 GLY_RADICAL_1 ; PS51149 GLY_RADICAL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEQFAKAWTGFADGEWQNEVNVRDFIQKNYAPYEGDEEFLVSEGTEATNKLWAKVMEGI
CCHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCEEEECCCHHHHHHHHHHHHHH
KQENATHAPVDFDTSLISTITSHDAGYINQDLETIVGLQTDAPLKRAIIPNGGVRMVEGS
HHCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCHHHEECCCCCEEEEECC
CKAYDRVLDPMVSKIYSEYRKTHNAGVFDIYTKDILNCRKSGVLTGLPDAYGRGRIIGDY
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHCCCCCCCCCCCCCCCEEHHH
RRVALYGIDFLITDKLAQFASLQERFEKGEDLTATMQLREEIAEQHRALGQIKVMAAKYG
HEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHEEEEEEHHC
CDISGPATTAQEAIQWTYFGYLAAVKSQNGAAMSLGRTSTFLDIYIQRDIEAGLLTEELA
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCEEEEEEEECCCCCCHHHHHHH
QEMIDHFVMKLRMVRFLRTPEYDELFSGDPIWATESMGGMGLDGRTLVTRTNFRFLNSLY
HHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCEEECCCCCCCCCCCEEEEECCHHHHHHHH
TMGPSPEPNITVLWSEQLPVGFKRFCAKVSIDTSSIQYENDDLMRPDLGSDDYAIACCVS
HCCCCCCCCEEEEECCCCCCCHHHHHHEEEECCCCEEECCCCCCCCCCCCCCCEEEEEHH
PMIVGKQMQFFGARANLAKTMLYSINGGVDEKLKIQIGPKEAPMTDEVLDYAKVMDRLDH
HHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHH
FMDWLATQYVTALNSIHYMHDKYSYEASLMALHDRDVRRTMACGIAGLSVAADSLSAIKY
HHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHEE
ATVKPIRDENGIAIDFDIEGDYPKFGNNDARVDDIACELVSTFMGKIRKLTMYRNAIPTQ
EEECCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
SILTITSNVVYGKKTGNTPDGRQAGAPFAPGANPMHGRDEKGAVASLTSVGKLPFADAQD
HHHEECCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCC
GISYTFSIVPNALGKDDDNRRSNLAGLMDGYFHHEANVEGGQHLNVNVLNRETLEDAVKH
CCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCHHHHHHHHHC
PENYPQLTIRVSGYAVRFNSLTTEQQADVIARTFTATL
CCCCCEEEEEEEEEEEEECCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AEQFAKAWTGFADGEWQNEVNVRDFIQKNYAPYEGDEEFLVSEGTEATNKLWAKVMEGI
CHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCEEEECCCHHHHHHHHHHHHHH
KQENATHAPVDFDTSLISTITSHDAGYINQDLETIVGLQTDAPLKRAIIPNGGVRMVEGS
HHCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCHHHEECCCCCEEEEECC
CKAYDRVLDPMVSKIYSEYRKTHNAGVFDIYTKDILNCRKSGVLTGLPDAYGRGRIIGDY
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHCCCCCCCCCCCCCCCEEHHH
RRVALYGIDFLITDKLAQFASLQERFEKGEDLTATMQLREEIAEQHRALGQIKVMAAKYG
HEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHEEEEEEHHC
CDISGPATTAQEAIQWTYFGYLAAVKSQNGAAMSLGRTSTFLDIYIQRDIEAGLLTEELA
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCEEEEEEEECCCCCCHHHHHHH
QEMIDHFVMKLRMVRFLRTPEYDELFSGDPIWATESMGGMGLDGRTLVTRTNFRFLNSLY
HHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCEEECCCCCCCCCCCEEEEECCHHHHHHHH
TMGPSPEPNITVLWSEQLPVGFKRFCAKVSIDTSSIQYENDDLMRPDLGSDDYAIACCVS
HCCCCCCCCEEEEECCCCCCCHHHHHHEEEECCCCEEECCCCCCCCCCCCCCCEEEEEHH
PMIVGKQMQFFGARANLAKTMLYSINGGVDEKLKIQIGPKEAPMTDEVLDYAKVMDRLDH
HHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHH
FMDWLATQYVTALNSIHYMHDKYSYEASLMALHDRDVRRTMACGIAGLSVAADSLSAIKY
HHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHEE
ATVKPIRDENGIAIDFDIEGDYPKFGNNDARVDDIACELVSTFMGKIRKLTMYRNAIPTQ
EEECCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
SILTITSNVVYGKKTGNTPDGRQAGAPFAPGANPMHGRDEKGAVASLTSVGKLPFADAQD
HHHEECCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCC
GISYTFSIVPNALGKDDDNRRSNLAGLMDGYFHHEANVEGGQHLNVNVLNRETLEDAVKH
CCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCHHHHHHHHHC
PENYPQLTIRVSGYAVRFNSLTTEQQADVIARTFTATL
CCCCCEEEEEEEEEEEEECCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3053170; 8905232; 9278503; 2651404; 1310545; 9629924; 10504733 [H]