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The map label for this gene is pflA [H]

Identifier: 209695413

GI number: 209695413

Start: 2091688

End: 2092425

Strand: Reverse

Name: pflA [H]

Synonym: VSAL_I1953

Alternate gene names: 209695413

Gene position: 2092425-2091688 (Counterclockwise)

Preceding gene: 209695414

Following gene: 209695412

Centisome position: 62.93

GC content: 39.97

Gene sequence:

>738_bases
ATGTCTGTAGGTCGTATTCACTCTTTTGAATCTTGTGGTACCGTTGATGGTCCTGGTATTCGCTTTATCATTTTTTTACA
AGGCTGCTTAATGCGTTGTATGTATTGCCATAATCGCGATACTTGGGATACACATGAAGGCAAAGAAGTCTCTGTGACTG
AATTAATCGAAGAAGCCAAAAGCTACCGTCACTTTATGAAAGCGTCAGGTGGCGGCATTACCTGTTCTGGTGGTGAAGCC
ATGCTTCAACCAGAATTCGTACGTGATTTCTTTCAAGCAGCACAAGCCGAAGGCATGCACACGTGTTTAGATACCAATGG
CTATATTCGTAAGCATACTGATGTCGTTGATGAAGTTCTTGCTGCTTCTGATCTTGTTATGCTTGATATTAAGCACATGA
AAGATGAAATCCACCACGATTTTATTGGTGTATCAAATAGACGAACATTGGACTTTGCTCGTTACCTGCATAAGATCGGT
AAAAAAACATGGATCCGTTACGTAATTGTTCCTGGCTACACTGATGATGAAGAATCAGCACACATGCTAGGTGATTTCAT
TAAAGATATGGATAACATAGAGAAAGTAGAATTACTTCCATACCACCAACTTGGTGCCCATAAATGGGAAGCAATGGGTT
TAAAATATCCGCTTGATGGTGTTAATCCTCCTTCAAAAGAGACAATGGAAAAAATGGTTGCTATCCTAAGCCAATATCAC
GATAACGTTAGATACTGA

Upstream 100 bases:

>100_bases
TCCCCTCTTAAATCCCCTAATTTCTCTGTGGTTTGCCCTACTTAATTATCCGAAAGTGTAATAAAATGGCCTCATAATTA
TAGTTCGGAGAAATGTCGTC

Downstream 100 bases:

>100_bases
TCGTCGTCTCACCCCTAAATTTATACAAAATGCCCGACGACGGGCATTTTTATTGGAATAAATAATGTATCAATTAAGCT
TTTCACTACAAGAATTTCTC

Product: pyruvate formate lyase-activating enzyme 1

Products: NA

Alternate protein names: Formate-C-acetyltransferase-activating enzyme 1; PFL-activating enzyme 1 [H]

Number of amino acids: Translated: 245; Mature: 244

Protein sequence:

>245_residues
MSVGRIHSFESCGTVDGPGIRFIIFLQGCLMRCMYCHNRDTWDTHEGKEVSVTELIEEAKSYRHFMKASGGGITCSGGEA
MLQPEFVRDFFQAAQAEGMHTCLDTNGYIRKHTDVVDEVLAASDLVMLDIKHMKDEIHHDFIGVSNRRTLDFARYLHKIG
KKTWIRYVIVPGYTDDEESAHMLGDFIKDMDNIEKVELLPYHQLGAHKWEAMGLKYPLDGVNPPSKETMEKMVAILSQYH
DNVRY

Sequences:

>Translated_245_residues
MSVGRIHSFESCGTVDGPGIRFIIFLQGCLMRCMYCHNRDTWDTHEGKEVSVTELIEEAKSYRHFMKASGGGITCSGGEA
MLQPEFVRDFFQAAQAEGMHTCLDTNGYIRKHTDVVDEVLAASDLVMLDIKHMKDEIHHDFIGVSNRRTLDFARYLHKIG
KKTWIRYVIVPGYTDDEESAHMLGDFIKDMDNIEKVELLPYHQLGAHKWEAMGLKYPLDGVNPPSKETMEKMVAILSQYH
DNVRY
>Mature_244_residues
SVGRIHSFESCGTVDGPGIRFIIFLQGCLMRCMYCHNRDTWDTHEGKEVSVTELIEEAKSYRHFMKASGGGITCSGGEAM
LQPEFVRDFFQAAQAEGMHTCLDTNGYIRKHTDVVDEVLAASDLVMLDIKHMKDEIHHDFIGVSNRRTLDFARYLHKIGK
KTWIRYVIVPGYTDDEESAHMLGDFIKDMDNIEKVELLPYHQLGAHKWEAMGLKYPLDGVNPPSKETMEKMVAILSQYHD
NVRY

Specific function: Activation of pyruvate formate-lyase 1 under anaerobic conditions by generation of an organic free radical, using S- adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine [H]

COG id: COG1180

COG function: function code O; Pyruvate-formate lyase-activating enzyme

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the organic radical-activating enzymes family [H]

Homologues:

Organism=Escherichia coli, GI1787130, Length=243, Percent_Identity=66.6666666666667, Blast_Score=366, Evalue=1e-103,
Organism=Escherichia coli, GI1790389, Length=260, Percent_Identity=26.5384615384615, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI226510931, Length=179, Percent_Identity=32.4022346368715, Blast_Score=76, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR012838
- InterPro:   IPR001989
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =1.97.1.4 [H]

Molecular weight: Translated: 28009; Mature: 27878

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: PS01087 RADICAL_ACTIVATING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
5.3 %Met     (Translated Protein)
7.8 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
7.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HCCCC
>Mature Secondary Structure 
SVGRIHSFESCGTVDGPGIRFIIFLQGCLMRCMYCHNRDTWDTHEGKEVSVTELIEEAK
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHH
SYRHFMKASGGGITCSGGEAMLQPEFVRDFFQAAQAEGMHTCLDTNGYIRKHTDVVDEVL
HHHHHHHCCCCCEEECCCCCCCCHHHHHHHHHHHHHCCCHHHCCCCCCHHHHHHHHHHHH
AASDLVMLDIKHMKDEIHHDFIGVSNRRTLDFARYLHKIGKKTWIRYVIVPGYTDDEESA
HHCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHEEEEEEECCCCCCHHHH
HMLGDFIKDMDNIEKVELLPYHQLGAHKWEAMGLKYPLDGVNPPSKETMEKMVAILSQYH
HHHHHHHHHHHCCCCEEECCCHHHCCCHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
DNVRY
HCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]