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The map label for this gene is cysB [H]

Identifier: 209695183

GI number: 209695183

Start: 1785450

End: 1786424

Strand: Reverse

Name: cysB [H]

Synonym: VSAL_I1689

Alternate gene names: 209695183

Gene position: 1786424-1785450 (Counterclockwise)

Preceding gene: 209695185

Following gene: 209695182

Centisome position: 53.72

GC content: 40.72

Gene sequence:

>975_bases
ATGAAATTACAACAACTGAAATACATTGTTGAAGTGGTTAATCACAGCCTTAATGTGTCTTCAACTGCAGAGTCTTTGTA
TACCTCTCAGCCTGGTATTAGTAAGCAAGTTCGATTGTTAGAAGATGAACTGGGTGTGCAAATCTTTGAACGAAGTGGCA
AGCACCTTACTCGAGTTACGCCATCGGGTGAAGAGATCGTAAAAATATCTCGCGAGATTTTATCTCGAGTTGACAGTATC
AAAGCGGTGGCTGGTGAGCATACTCACCCAGAGAAAGGGTTGTTGAACATTACGACGACGCATACTCAGGCTCGTTATGC
TTTGCCGGATGTGATTAAAGGTTTTACTTCTCGTTATCCTCAAGTTTCTTTGCATATGCACCAAGGCACGCCAACGCAAA
TGTCAGATGCAATTGCAAAAGGCACGGCGGATTTTGCAATTGCAACAGAAGCGCTTCATTTATACCACGATGCCATTATG
TTGCCTTGTTATCACTGGAACCGCTCGATAGTAGTAAATAAGGATCATCCACTGGCACAGAAAAAACACGTGACAGTTCA
CGATCTTGCTGCTTATGAATTGGTTACTTATGTGTTTGGTTTTACTGGACGCTCGGAGTTAGACAGTGCGTTTAATAAAG
CAGGGTTAACACCTAAAATTGTCTTTACGGCTACCGATGCTGATGTCATTAAAACTTATGTTCGTTTAGGTATTGGTGTT
GGTGTTATAGCAAGCATGGCAATGGATCCTGAAAGAGATTCTGATCTAGTTGCTATAGATGCAAGTCATATATTTGATGC
AAGTACAACAACGATTGGCTTTAAGCGTGGTTCATTCCTAAAGAGTTATATGTATGACTTTATGGAACGTTTTGCACCAC
ATCTCACTCGCCCAATCGTAGAGCAAGCAACCATGCTTAAAAATAATTATGAAATAGAAGAGATGTTTAAAGACATTAAC
CTTCCTGTCCGTTAA

Upstream 100 bases:

>100_bases
ATTAAAGTTGATAAGGCTTTGAAAATAGGACTAAATAAGGATATAACTATATCGAATAAGTGACATAATTTTCGATGTAA
AGGATTACAGGAATAAAATA

Downstream 100 bases:

>100_bases
GATCGCCTCGATTTAATTTCTAGGGTGTCATCGGATGCAATCTCAGTTTTTACATATCGACCGTTTGTTAACTCAGACGG
TCTTTTTTTGGCAATTTTCT

Product: transcriptional regulator CysB

Products: NA

Alternate protein names: Cys regulon transcriptional activator [H]

Number of amino acids: Translated: 324; Mature: 324

Protein sequence:

>324_residues
MKLQQLKYIVEVVNHSLNVSSTAESLYTSQPGISKQVRLLEDELGVQIFERSGKHLTRVTPSGEEIVKISREILSRVDSI
KAVAGEHTHPEKGLLNITTTHTQARYALPDVIKGFTSRYPQVSLHMHQGTPTQMSDAIAKGTADFAIATEALHLYHDAIM
LPCYHWNRSIVVNKDHPLAQKKHVTVHDLAAYELVTYVFGFTGRSELDSAFNKAGLTPKIVFTATDADVIKTYVRLGIGV
GVIASMAMDPERDSDLVAIDASHIFDASTTTIGFKRGSFLKSYMYDFMERFAPHLTRPIVEQATMLKNNYEIEEMFKDIN
LPVR

Sequences:

>Translated_324_residues
MKLQQLKYIVEVVNHSLNVSSTAESLYTSQPGISKQVRLLEDELGVQIFERSGKHLTRVTPSGEEIVKISREILSRVDSI
KAVAGEHTHPEKGLLNITTTHTQARYALPDVIKGFTSRYPQVSLHMHQGTPTQMSDAIAKGTADFAIATEALHLYHDAIM
LPCYHWNRSIVVNKDHPLAQKKHVTVHDLAAYELVTYVFGFTGRSELDSAFNKAGLTPKIVFTATDADVIKTYVRLGIGV
GVIASMAMDPERDSDLVAIDASHIFDASTTTIGFKRGSFLKSYMYDFMERFAPHLTRPIVEQATMLKNNYEIEEMFKDIN
LPVR
>Mature_324_residues
MKLQQLKYIVEVVNHSLNVSSTAESLYTSQPGISKQVRLLEDELGVQIFERSGKHLTRVTPSGEEIVKISREILSRVDSI
KAVAGEHTHPEKGLLNITTTHTQARYALPDVIKGFTSRYPQVSLHMHQGTPTQMSDAIAKGTADFAIATEALHLYHDAIM
LPCYHWNRSIVVNKDHPLAQKKHVTVHDLAAYELVTYVFGFTGRSELDSAFNKAGLTPKIVFTATDADVIKTYVRLGIGV
GVIASMAMDPERDSDLVAIDASHIFDASTTTIGFKRGSFLKSYMYDFMERFAPHLTRPIVEQATMLKNNYEIEEMFKDIN
LPVR

Specific function: This protein is a positive regulator of gene expression for the cysteine regulon. The inducer for cysB is N-acetylserine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787530, Length=322, Percent_Identity=75.7763975155279, Blast_Score=527, Evalue=1e-151,
Organism=Escherichia coli, GI1788296, Length=313, Percent_Identity=39.6166134185303, Blast_Score=255, Evalue=2e-69,
Organism=Escherichia coli, GI1790399, Length=287, Percent_Identity=28.2229965156794, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI157672245, Length=235, Percent_Identity=27.6595744680851, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI1788887, Length=246, Percent_Identity=28.0487804878049, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI145693105, Length=203, Percent_Identity=24.1379310344828, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI1790262, Length=218, Percent_Identity=25.2293577981651, Blast_Score=74, Evalue=9e-15,
Organism=Escherichia coli, GI1787879, Length=256, Percent_Identity=26.5625, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1787806, Length=283, Percent_Identity=23.6749116607774, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788748, Length=258, Percent_Identity=22.8682170542636, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1789204, Length=233, Percent_Identity=24.0343347639485, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1787601, Length=280, Percent_Identity=22.8571428571429, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 36235; Mature: 36235

Theoretical pI: Translated: 7.09; Mature: 7.09

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLQQLKYIVEVVNHSLNVSSTAESLYTSQPGISKQVRLLEDELGVQIFERSGKHLTRVT
CCHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEC
PSGEEIVKISREILSRVDSIKAVAGEHTHPEKGLLNITTTHTQARYALPDVIKGFTSRYP
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCC
QVSLHMHQGTPTQMSDAIAKGTADFAIATEALHLYHDAIMLPCYHWNRSIVVNKDHPLAQ
EEEEEEECCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHEEEEECCCEEEEECCCCCCC
KKHVTVHDLAAYELVTYVFGFTGRSELDSAFNKAGLTPKIVFTATDADVIKTYVRLGIGV
CCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHH
GVIASMAMDPERDSDLVAIDASHIFDASTTTIGFKRGSFLKSYMYDFMERFAPHLTRPIV
HHHHHHHCCCCCCCCEEEEEHHHHCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHH
EQATMLKNNYEIEEMFKDINLPVR
HHHHHHHCCCCHHHHHHHCCCCCC
>Mature Secondary Structure
MKLQQLKYIVEVVNHSLNVSSTAESLYTSQPGISKQVRLLEDELGVQIFERSGKHLTRVT
CCHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEC
PSGEEIVKISREILSRVDSIKAVAGEHTHPEKGLLNITTTHTQARYALPDVIKGFTSRYP
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCC
QVSLHMHQGTPTQMSDAIAKGTADFAIATEALHLYHDAIMLPCYHWNRSIVVNKDHPLAQ
EEEEEEECCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHEEEEECCCEEEEECCCCCCC
KKHVTVHDLAAYELVTYVFGFTGRSELDSAFNKAGLTPKIVFTATDADVIKTYVRLGIGV
CCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHH
GVIASMAMDPERDSDLVAIDASHIFDASTTTIGFKRGSFLKSYMYDFMERFAPHLTRPIV
HHHHHHHCCCCCCCCEEEEEHHHHCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHH
EQATMLKNNYEIEEMFKDINLPVR
HHHHHHHCCCCHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]