Click here to switch to the map view.

The map label for this gene is gcvA [H]

Identifier: 209694794

GI number: 209694794

Start: 1359896

End: 1360810

Strand: Reverse

Name: gcvA [H]

Synonym: VSAL_I1252

Alternate gene names: 209694794

Gene position: 1360810-1359896 (Counterclockwise)

Preceding gene: 209694800

Following gene: 209694790

Centisome position: 40.92

GC content: 38.36

Gene sequence:

>915_bases
ATGGCCATTAATCATAAAAATATTCCTTCTCTGAGTTGCCTAATTGTATTTGAAGCAGCTTCTAGGTTAGGAACGTTGAC
TAAAGCGGCTGATGAATTATGTGTCACCCCTACAGCGGTTAGTAAGCAAATAAAAAAGTTAGAAGCCTTTTTAAATACTC
AGTTATTCACTCGCGGTAAACAAGGTGTAGAGCTAACCCCTAAAGGCGCACTTTATTTAAACAGTGTGATTGAAGCGTTA
GAGATACTATCAAGTCAGCGCATACCAATGGATGATGAACATAACCCCCTCCCCCTAAATATAGAAATAGGAACGTGTTT
TTCTCACTTTTGGTTGCTGCCTAAATTGGATGATTTTCGTAATAAATACCCCGAGATCACATTGAATTTAGTGATTAATA
ATGACAGGAACATTGGCGATAATGCGGATTATGATATGGCCTTTTTCTATTCATCTATGGATTCAATCAATAAAAATAAT
CATTTACTCTTTGCTGAGAAAGTCCTACTCGTATGCAGTCCCGATTTTCTAAAAAAACGACCTGAATGTACTGATTTATC
TCAGCTTTGGCAGCAGCCTGTTATTATGCTGAAAGAGACCTTATCATTTTGGGAAGATTGGCAATCGTGGGCTGATGTGT
ATGGAATCGAATACCAACCGCCTCAAAATGTTATTCAAGTTGAGGATCAAGTTTCCGTTATTCAAGCCGCGGTTAATGAT
GCGGGAATTGCACTAGCTTGGGATTGGCATGTAAGGGAATTAATAGAACAAGGGAGGTTGGTTGCGTTAACCAAGCCGGT
TGAATTTAACACGAATGCCTTCTTTTTATCGGTTTCAGAAGACAGTGATCATTTCGCTTCTCAACTCTTTGTAGATTGGA
TTATTTCTACTGAACGTGCCTATTTGAATTCATAA

Upstream 100 bases:

>100_bases
ATAATAGAACTGATATCAACATCATAGAAGGCTAGCGTTGTGCGTAAACCGCTTTTTAGTTAACATATCCTTAACTAAAA
GTTCAGAATAGGTGTCAGTC

Downstream 100 bases:

>100_bases
AAAAGCCCTAATAGACGTATTATTCCATTAAGGCTTTCGTTGTGTATGTTTGTTGTTCACATCAATTTACAAGAATCAGT
TACGTAATGATTTCATCTGT

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 304; Mature: 303

Protein sequence:

>304_residues
MAINHKNIPSLSCLIVFEAASRLGTLTKAADELCVTPTAVSKQIKKLEAFLNTQLFTRGKQGVELTPKGALYLNSVIEAL
EILSSQRIPMDDEHNPLPLNIEIGTCFSHFWLLPKLDDFRNKYPEITLNLVINNDRNIGDNADYDMAFFYSSMDSINKNN
HLLFAEKVLLVCSPDFLKKRPECTDLSQLWQQPVIMLKETLSFWEDWQSWADVYGIEYQPPQNVIQVEDQVSVIQAAVND
AGIALAWDWHVRELIEQGRLVALTKPVEFNTNAFFLSVSEDSDHFASQLFVDWIISTERAYLNS

Sequences:

>Translated_304_residues
MAINHKNIPSLSCLIVFEAASRLGTLTKAADELCVTPTAVSKQIKKLEAFLNTQLFTRGKQGVELTPKGALYLNSVIEAL
EILSSQRIPMDDEHNPLPLNIEIGTCFSHFWLLPKLDDFRNKYPEITLNLVINNDRNIGDNADYDMAFFYSSMDSINKNN
HLLFAEKVLLVCSPDFLKKRPECTDLSQLWQQPVIMLKETLSFWEDWQSWADVYGIEYQPPQNVIQVEDQVSVIQAAVND
AGIALAWDWHVRELIEQGRLVALTKPVEFNTNAFFLSVSEDSDHFASQLFVDWIISTERAYLNS
>Mature_303_residues
AINHKNIPSLSCLIVFEAASRLGTLTKAADELCVTPTAVSKQIKKLEAFLNTQLFTRGKQGVELTPKGALYLNSVIEALE
ILSSQRIPMDDEHNPLPLNIEIGTCFSHFWLLPKLDDFRNKYPEITLNLVINNDRNIGDNADYDMAFFYSSMDSINKNNH
LLFAEKVLLVCSPDFLKKRPECTDLSQLWQQPVIMLKETLSFWEDWQSWADVYGIEYQPPQNVIQVEDQVSVIQAAVNDA
GIALAWDWHVRELIEQGRLVALTKPVEFNTNAFFLSVSEDSDHFASQLFVDWIISTERAYLNS

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=303, Percent_Identity=32.3432343234323, Blast_Score=124, Evalue=8e-30,
Organism=Escherichia coli, GI1788706, Length=255, Percent_Identity=28.6274509803922, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1786448, Length=290, Percent_Identity=25.8620689655172, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1786401, Length=291, Percent_Identity=25.7731958762887, Blast_Score=81, Evalue=8e-17,
Organism=Escherichia coli, GI1789440, Length=258, Percent_Identity=29.4573643410853, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1786508, Length=259, Percent_Identity=26.6409266409266, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI157672245, Length=224, Percent_Identity=25.4464285714286, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1790262, Length=259, Percent_Identity=25.0965250965251, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34617; Mature: 34486

Theoretical pI: Translated: 4.49; Mature: 4.49

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAINHKNIPSLSCLIVFEAASRLGTLTKAADELCVTPTAVSKQIKKLEAFLNTQLFTRGK
CCCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCC
QGVELTPKGALYLNSVIEALEILSSQRIPMDDEHNPLPLNIEIGTCFSHFWLLPKLDDFR
CCCEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCHHHHHCCCCCCHHHH
NKYPEITLNLVINNDRNIGDNADYDMAFFYSSMDSINKNNHLLFAEKVLLVCSPDFLKKR
CCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEEEEEEECCHHHHCC
PECTDLSQLWQQPVIMLKETLSFWEDWQSWADVYGIEYQPPQNVIQVEDQVSVIQAAVND
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEHHHHHHHHHHHHCC
AGIALAWDWHVRELIEQGRLVALTKPVEFNTNAFFLSVSEDSDHFASQLFVDWIISTERA
CCEEEEEHHHHHHHHHCCCEEEEECCEEECCCEEEEEECCCCHHHHHHHHHHHHHHHHHH
YLNS
HCCC
>Mature Secondary Structure 
AINHKNIPSLSCLIVFEAASRLGTLTKAADELCVTPTAVSKQIKKLEAFLNTQLFTRGK
CCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCC
QGVELTPKGALYLNSVIEALEILSSQRIPMDDEHNPLPLNIEIGTCFSHFWLLPKLDDFR
CCCEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCHHHHHCCCCCCHHHH
NKYPEITLNLVINNDRNIGDNADYDMAFFYSSMDSINKNNHLLFAEKVLLVCSPDFLKKR
CCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEEEEEEECCHHHHCC
PECTDLSQLWQQPVIMLKETLSFWEDWQSWADVYGIEYQPPQNVIQVEDQVSVIQAAVND
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEHHHHHHHHHHHHCC
AGIALAWDWHVRELIEQGRLVALTKPVEFNTNAFFLSVSEDSDHFASQLFVDWIISTERA
CCEEEEEHHHHHHHHHCCCEEEEECCEEECCCEEEEEECCCCHHHHHHHHHHHHHHHHHH
YLNS
HCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]