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The map label for this gene is braB [H]

Identifier: 209694764

GI number: 209694764

Start: 1326995

End: 1328293

Strand: Direct

Name: braB [H]

Synonym: VSAL_I1220

Alternate gene names: 209694764

Gene position: 1326995-1328293 (Clockwise)

Preceding gene: 209694763

Following gene: 209694765

Centisome position: 39.91

GC content: 42.88

Gene sequence:

>1299_bases
TTGAAAAAACACAACTTGCTTGCGATTGGTCTAATGACGTTTGCCATGTTCCTTGGTGCAGGAAACATCATATTCCCTCC
TTTTCTTGGCCTTGAAGCAGGCACTCAATTTCTCCCTGCAATGCTCGGCTTTTTACTTACCGCGGTTGGACTTCCAGCAT
TAACGCTGATTGTTTTAGGGCGACTGAGTAACAGTGGTCAACTAACTCAAGCGTTACCGAAACCATTGGCGATTGCTTTT
TGGGTATTACTTTTTATTGCAATTGGTCCTGCCTTCGGGATGCCACGAGCAGTAACGGTTGCGTATGAAATGGGGATTAA
GCCCTTCTTTACCGGTGACCACCTAATGCTTTTCTCGGTTATGTTTGTGGGAGCTACGTTATTGTTGGCTTTCCAAAGAG
GTAAGTTGGTGGATTACATTGGTAAAGTCATGACGCCACTGCTTGTTCTTATGCTAGCCGCGTTAGCGTTAGCGGCCATT
ATTACGCCATTAGGTGTTCTTGGTGAGCCGAGTGCCAGTTATCAGCATAAAGCCATCACAAGCGGTTTGATCCAAGGTTA
CATGACAATGGATGCGATTGCGGCTGTTGGATTTGGTTGGGTCATCATTAAAGCCATTCGTGATAAAGGGTGTGAATCAC
CAAAAGATGTTTTTCAAGCAACGCTTAAAGTAACGTTGATTTATGCGCTGCTAATGTCGGCGTGTTATTTAGCCATGGCT
TATGTTGGTGCGACATCAACGTCTATTGCTGAAGGCGCAACTAATGGTGGTGAATTACTAACGCGTTATGTTGCTGAGAT
ATTTGGACCACTAGGTCAATACTTATTAGCAGGTATTATTATTATGGCGTGTTTAACGACGACGGTAGGGTTGACGAATG
CATGTGCTGAGTACAGCCAACAAACGTTCCGCACTCCGTTTGCGATTACCGCGATTATCGTTACAGCGATGACAGGCGTT
GTTGCTAACTTCGGATTAGAGCAAATCTTGGCTATCAGCTTACCTGCTATACTCATATTATGTCCGGTAGCGATTGCTTT
AGTATTAGCGGCGTGGTTTTTACCTACGACAAAACACAATACTATTTCATACGTTAGTGTTGTGATGATTAGCGTTGTAT
TTGGTGGGTTAGATGCACTGCATATTTTGGGTTTATTGTCTGATTCTTTATCTGCATCATTAACTCATTATATTCCTCTC
TTTTCGGCTCATGCGAGTTGGTTTTTACCTGTAGTGATAACTGTATTTATTAGTCTATTACTAAACCGAAAATCGAGTGC
ATTAGAGTCATCGAACTAA

Upstream 100 bases:

>100_bases
GACGGAGGACTTCTTAATTTTCTTCTTCTTTTTTCAGCTAATCTGACGGTCCACAATAAAGCTAATAGGGCACAGCCCAA
TAGCAATAAGGACTTGTACA

Downstream 100 bases:

>100_bases
GACTAGATAAAGGCAAATAAAGCGGTTAATAGATAAGATCCCTATGAGTTTACATTAGGGATCATTCACTATTGAGAATG
TGCTTAAATCGCTAATTTAT

Product: branched chain amino acid transport system II carrier protein

Products: L-leucine [Cytoplasm]; L-valine [Cytoplasm]; Na (I) [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: LIV-II [H]

Number of amino acids: Translated: 432; Mature: 432

Protein sequence:

>432_residues
MKKHNLLAIGLMTFAMFLGAGNIIFPPFLGLEAGTQFLPAMLGFLLTAVGLPALTLIVLGRLSNSGQLTQALPKPLAIAF
WVLLFIAIGPAFGMPRAVTVAYEMGIKPFFTGDHLMLFSVMFVGATLLLAFQRGKLVDYIGKVMTPLLVLMLAALALAAI
ITPLGVLGEPSASYQHKAITSGLIQGYMTMDAIAAVGFGWVIIKAIRDKGCESPKDVFQATLKVTLIYALLMSACYLAMA
YVGATSTSIAEGATNGGELLTRYVAEIFGPLGQYLLAGIIIMACLTTTVGLTNACAEYSQQTFRTPFAITAIIVTAMTGV
VANFGLEQILAISLPAILILCPVAIALVLAAWFLPTTKHNTISYVSVVMISVVFGGLDALHILGLLSDSLSASLTHYIPL
FSAHASWFLPVVITVFISLLLNRKSSALESSN

Sequences:

>Translated_432_residues
MKKHNLLAIGLMTFAMFLGAGNIIFPPFLGLEAGTQFLPAMLGFLLTAVGLPALTLIVLGRLSNSGQLTQALPKPLAIAF
WVLLFIAIGPAFGMPRAVTVAYEMGIKPFFTGDHLMLFSVMFVGATLLLAFQRGKLVDYIGKVMTPLLVLMLAALALAAI
ITPLGVLGEPSASYQHKAITSGLIQGYMTMDAIAAVGFGWVIIKAIRDKGCESPKDVFQATLKVTLIYALLMSACYLAMA
YVGATSTSIAEGATNGGELLTRYVAEIFGPLGQYLLAGIIIMACLTTTVGLTNACAEYSQQTFRTPFAITAIIVTAMTGV
VANFGLEQILAISLPAILILCPVAIALVLAAWFLPTTKHNTISYVSVVMISVVFGGLDALHILGLLSDSLSASLTHYIPL
FSAHASWFLPVVITVFISLLLNRKSSALESSN
>Mature_432_residues
MKKHNLLAIGLMTFAMFLGAGNIIFPPFLGLEAGTQFLPAMLGFLLTAVGLPALTLIVLGRLSNSGQLTQALPKPLAIAF
WVLLFIAIGPAFGMPRAVTVAYEMGIKPFFTGDHLMLFSVMFVGATLLLAFQRGKLVDYIGKVMTPLLVLMLAALALAAI
ITPLGVLGEPSASYQHKAITSGLIQGYMTMDAIAAVGFGWVIIKAIRDKGCESPKDVFQATLKVTLIYALLMSACYLAMA
YVGATSTSIAEGATNGGELLTRYVAEIFGPLGQYLLAGIIIMACLTTTVGLTNACAEYSQQTFRTPFAITAIIVTAMTGV
VANFGLEQILAISLPAILILCPVAIALVLAAWFLPTTKHNTISYVSVVMISVVFGGLDALHILGLLSDSLSASLTHYIPL
FSAHASWFLPVVITVFISLLLNRKSSALESSN

Specific function: Component of the LIV-II transport system for branched- chain amino acids. BraB is specific for isoleucine, leucine and valine. The LIV-II transport system is coupled to sodium and lithium ions [H]

COG id: COG1114

COG function: function code E; Branched-chain amino acid permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the branched chain amino acid transporter family [H]

Homologues:

Organism=Escherichia coli, GI1786601, Length=440, Percent_Identity=35.9090909090909, Blast_Score=260, Evalue=1e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004685 [H]

Pfam domain/function: PF05525 Branch_AA_trans [H]

EC number: NA

Molecular weight: Translated: 45760; Mature: 45760

Theoretical pI: Translated: 8.45; Mature: 8.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKHNLLAIGLMTFAMFLGAGNIIFPPFLGLEAGTQFLPAMLGFLLTAVGLPALTLIVLG
CCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLSNSGQLTQALPKPLAIAFWVLLFIAIGPAFGMPRAVTVAYEMGIKPFFTGDHLMLFSV
HCCCCCCHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCHHHHHHH
MFVGATLLLAFQRGKLVDYIGKVMTPLLVLMLAALALAAIITPLGVLGEPSASYQHKAIT
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHH
SGLIQGYMTMDAIAAVGFGWVIIKAIRDKGCESPKDVFQATLKVTLIYALLMSACYLAMA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
YVGATSTSIAEGATNGGELLTRYVAEIFGPLGQYLLAGIIIMACLTTTVGLTNACAEYSQ
HHCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QTFRTPFAITAIIVTAMTGVVANFGLEQILAISLPAILILCPVAIALVLAAWFLPTTKHN
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
TISYVSVVMISVVFGGLDALHILGLLSDSLSASLTHYIPLFSAHASWFLPVVITVFISLL
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LNRKSSALESSN
HHCCHHHCCCCC
>Mature Secondary Structure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HHCCHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-leucine [Periplasm]; L-valine [Periplasm]; Na (I) [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-leucine [Periplasm] = Na (I) [Cytoplasm] + L-leucine [Cytoplasm] Na (I) [Periplasm] + L-valine [Periplasm] = Na (I) [Cytoplasm] + L-valine [Cytoplasm] Na (I) [Periplasm] + L-iso-leucine [Periplasm] = Na (I) [Cytoplasm] + L-iso-leuci

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2111004; 10984043 [H]