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The map label for this gene is topA [H]

Identifier: 209694713

GI number: 209694713

Start: 1266401

End: 1269040

Strand: Direct

Name: topA [H]

Synonym: VSAL_I1164

Alternate gene names: 209694713

Gene position: 1266401-1269040 (Clockwise)

Preceding gene: 209694712

Following gene: 209694715

Centisome position: 38.09

GC content: 42.99

Gene sequence:

>2640_bases
ATGGGTAAATCTCTAGTTATCGTGGAGTCACCAGCCAAAGCTAAAACTATTAACAAATACCTTGGCAAAGACTTCATCGT
AAAATCCAGTGTAGGTCACGTTCGTGATTTGCCTATGGGTTCGACAAGCACCGGAAAGAAAACGGCGGCAGCGTCAACCA
AAGGTATGAGTGTTGAAGATAAAGCACGCATTAAGAAAGAAAGAGACAAGAAGAATCTGATCAATAAAATGGGGATTGAC
CCATATAACGATTGGGCGGCGAATTACCAAATTCTTCCTGGCAAAGAAAAAGTCGTAGCAGAATTACAAAAACTGGCTGA
AAATGCAGACACTATCTATCTCGCAACCGATTTAGACCGTGAAGGGGAAGCTATTGCGTGGCACCTTCGCGAGATCATCG
GTGGTGATGATTCACGCTATAAACGAGTAGTTTTTAACGAAATTACAAAAAATGCGATTCAACAAGCGTTTGAAACTCCA
GGCGAACTAAGTATGCCAGGGGTTAACGCACAGCAAGCACGTCGTTTCTTAGATCGTGTTGTAGGCTTCATGGCTTCTCC
GCTATTGTGGAAAAAAGTGGCTCGTGGTTTGTCTGCGGGTCGTGTTCAATCCGTTGCCGTGAAAGTTTTGGTTGAGCGTG
AGCGTGAAATCAATGCCTTTATTCCTGAAGAGTTCTGGGATATTCATGCAGATACACTGACGTCTGGAAAAGAAAATTTC
CGTCTGCAAGTTGCACAGCGTAATGGTTCGGTATTTAAACCACTGAATCAAGCGGATACAGAATCAGCGGTAAGTATTCT
TGAAAAAGCGGAATACGAAGTTTGTAAACGTGAAGACCGCCCAACGAAAAGTAAGCCATCGGCACCTTATATCACGTCAA
CACTGCAACAAGCAGCAAGTACACGCTTAGGTTACGGTGTGAAAAAGACCATGATGCTAGCTCAGCGCCTCTATGAGGGC
GGTTACATCACTTATATGCGTACTGACTCAACTAACTTAAGTAAAGAAGCTGTAGAGACGTTACGTGAGTTTATTGGTTC
TGAGTATGGCGAGAGCTATTTACCGGCTGCTCCAATTATTTATGGAAGCAAAGAAGGCGCACAAGAAGCTCACGAAGCGA
TTCGCCCTTCAAGTGTGGATGTGAAAGTAGATGACCTGCAAGGTATGGAAGCCGACGCTCATAAACTTTACAACCTAATT
TGGAATCAATTTGTGTCATGTCAAATGACGCCAGCACAATATGATTCAACCACATTGAGCGTAAAAGCCGATGAATTCAC
CTTAAAAGCGAAAGGTCGTATCCTTAAGTTTGATGGTTGGACTCGCGTGCAACGTCCAATGGGTAAAAATGAAGATCAAT
TGCTTCCTGCCGTTCAACTTGGCGATAAGCTAAAACTAGAACAGCTTGATCCGAAACAACACTTTACTAAGCCACCAGCG
CGTTTCACAGAAGCCGCATTGGTTAAAGAACTTGAGAAAAAAGGCATTGGTCGTCCATCGACTTACGCATCAATCATCTC
AACGATTCAAGACCGTGGCTACGTTCGTGTTGAAAGCCGTCGTTTCTATGCAGAAAAAATGGGCGAAATTGTTACTGACC
GTCTAAATCAATCGTTTGATGATTTGATGGATTACGATTTTACTGCTCGTATGGAAGGCAATTTGGATAAGATTGCTGAA
GGCAGCAAAGATTGGAAAGAAGTGCTAAATGCGTTCTTCCAAGACTTCACTGACGATATAGCAAAAGCTGAGCTTGATGA
ATCAGAAGGCGGCATGTCACCAAATAATATCGTAGAAACTGATATTAAATGTCCGACGTGTGACCGTAACATGGGTATTC
GTACTGCATCAACTGGGGTATTCCTTGGTTGTTCAGGTTACGCATTACCGCCAAAAGAGCGCTGTAAGAAAACGATTAAC
TTAGGTAATGAAGAGGGCATTATTAATGTTCTTGAAGAAGACGTAGAAACGGCCGCATTACGAGCCAAAAAACGTTGTCC
TAAGTGTGAAACAGCAATGGATCCTTACCTAATCGATCAAGATCGTAAACTGCATGTTTGTGGTAATAACCCGAACTGTG
ATGGTTATATTGTTGAGAAAGGTGAGTTCCAACTTAAAGGCTATGATGGACCCATTGTTGAGTGTGACAAATGTGGTTCT
GATATGGAACTTAAGAACGGTCGCTTTGGTAAATACATGGATTGTACGAGTGAGACGTGTAAAAACACACGTAAGATCCT
AAGAAATGGCGATGTTGCGCCACCAAAAGAAGATCCAGTACACCTTCCTGAATTGGAATGTGAAAAATCTGATGCGTACT
TTGTGTTGCGTGATGGTGCATCTGGGTTATTCTTAGCGGCAAGTACCTTCCCTAAATCGCGTGAAACACGAGCGCCATTA
GTGGAAGAGTTAGTTCGCTTTAAAGATCGTTTATCGCCTAAGTTTGTGTACTTAACGGAAGCGCCAACGCACGATCCAGA
CGGTAAACCAGCCGTAATTCGTTTTAGCCGTAAGACTAAAGAAAATTACGTTCGTACTGAAACTGATGGTAAGCCATCAG
GTTGGACGGCGTTATATCTTGATGGGAAATGGGCTGTTACCGATAAGCGTAAAAAGCCAAAAGCTGAGAAAGAGTCTTAA

Upstream 100 bases:

>100_bases
CCCTCAATAGTAATATATAGATAAAATCTCAATTTATAGCTGTGGATAATATTATTTTCGACAGTTTTGTATTTCAGCAC
GAGGGCGTTAGTGAGACATT

Downstream 100 bases:

>100_bases
TAATTAAAGACATTATAATCAGAAATAAATAGATGAGAGCGGCAGAAATGCGGCTCTTTTTTATGATACCACTGTCACAA
AATTTCATATTTATTCTTAT

Product: DNA topoisomerase I

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]

Number of amino acids: Translated: 879; Mature: 878

Protein sequence:

>879_residues
MGKSLVIVESPAKAKTINKYLGKDFIVKSSVGHVRDLPMGSTSTGKKTAAASTKGMSVEDKARIKKERDKKNLINKMGID
PYNDWAANYQILPGKEKVVAELQKLAENADTIYLATDLDREGEAIAWHLREIIGGDDSRYKRVVFNEITKNAIQQAFETP
GELSMPGVNAQQARRFLDRVVGFMASPLLWKKVARGLSAGRVQSVAVKVLVEREREINAFIPEEFWDIHADTLTSGKENF
RLQVAQRNGSVFKPLNQADTESAVSILEKAEYEVCKREDRPTKSKPSAPYITSTLQQAASTRLGYGVKKTMMLAQRLYEG
GYITYMRTDSTNLSKEAVETLREFIGSEYGESYLPAAPIIYGSKEGAQEAHEAIRPSSVDVKVDDLQGMEADAHKLYNLI
WNQFVSCQMTPAQYDSTTLSVKADEFTLKAKGRILKFDGWTRVQRPMGKNEDQLLPAVQLGDKLKLEQLDPKQHFTKPPA
RFTEAALVKELEKKGIGRPSTYASIISTIQDRGYVRVESRRFYAEKMGEIVTDRLNQSFDDLMDYDFTARMEGNLDKIAE
GSKDWKEVLNAFFQDFTDDIAKAELDESEGGMSPNNIVETDIKCPTCDRNMGIRTASTGVFLGCSGYALPPKERCKKTIN
LGNEEGIINVLEEDVETAALRAKKRCPKCETAMDPYLIDQDRKLHVCGNNPNCDGYIVEKGEFQLKGYDGPIVECDKCGS
DMELKNGRFGKYMDCTSETCKNTRKILRNGDVAPPKEDPVHLPELECEKSDAYFVLRDGASGLFLAASTFPKSRETRAPL
VEELVRFKDRLSPKFVYLTEAPTHDPDGKPAVIRFSRKTKENYVRTETDGKPSGWTALYLDGKWAVTDKRKKPKAEKES

Sequences:

>Translated_879_residues
MGKSLVIVESPAKAKTINKYLGKDFIVKSSVGHVRDLPMGSTSTGKKTAAASTKGMSVEDKARIKKERDKKNLINKMGID
PYNDWAANYQILPGKEKVVAELQKLAENADTIYLATDLDREGEAIAWHLREIIGGDDSRYKRVVFNEITKNAIQQAFETP
GELSMPGVNAQQARRFLDRVVGFMASPLLWKKVARGLSAGRVQSVAVKVLVEREREINAFIPEEFWDIHADTLTSGKENF
RLQVAQRNGSVFKPLNQADTESAVSILEKAEYEVCKREDRPTKSKPSAPYITSTLQQAASTRLGYGVKKTMMLAQRLYEG
GYITYMRTDSTNLSKEAVETLREFIGSEYGESYLPAAPIIYGSKEGAQEAHEAIRPSSVDVKVDDLQGMEADAHKLYNLI
WNQFVSCQMTPAQYDSTTLSVKADEFTLKAKGRILKFDGWTRVQRPMGKNEDQLLPAVQLGDKLKLEQLDPKQHFTKPPA
RFTEAALVKELEKKGIGRPSTYASIISTIQDRGYVRVESRRFYAEKMGEIVTDRLNQSFDDLMDYDFTARMEGNLDKIAE
GSKDWKEVLNAFFQDFTDDIAKAELDESEGGMSPNNIVETDIKCPTCDRNMGIRTASTGVFLGCSGYALPPKERCKKTIN
LGNEEGIINVLEEDVETAALRAKKRCPKCETAMDPYLIDQDRKLHVCGNNPNCDGYIVEKGEFQLKGYDGPIVECDKCGS
DMELKNGRFGKYMDCTSETCKNTRKILRNGDVAPPKEDPVHLPELECEKSDAYFVLRDGASGLFLAASTFPKSRETRAPL
VEELVRFKDRLSPKFVYLTEAPTHDPDGKPAVIRFSRKTKENYVRTETDGKPSGWTALYLDGKWAVTDKRKKPKAEKES
>Mature_878_residues
GKSLVIVESPAKAKTINKYLGKDFIVKSSVGHVRDLPMGSTSTGKKTAAASTKGMSVEDKARIKKERDKKNLINKMGIDP
YNDWAANYQILPGKEKVVAELQKLAENADTIYLATDLDREGEAIAWHLREIIGGDDSRYKRVVFNEITKNAIQQAFETPG
ELSMPGVNAQQARRFLDRVVGFMASPLLWKKVARGLSAGRVQSVAVKVLVEREREINAFIPEEFWDIHADTLTSGKENFR
LQVAQRNGSVFKPLNQADTESAVSILEKAEYEVCKREDRPTKSKPSAPYITSTLQQAASTRLGYGVKKTMMLAQRLYEGG
YITYMRTDSTNLSKEAVETLREFIGSEYGESYLPAAPIIYGSKEGAQEAHEAIRPSSVDVKVDDLQGMEADAHKLYNLIW
NQFVSCQMTPAQYDSTTLSVKADEFTLKAKGRILKFDGWTRVQRPMGKNEDQLLPAVQLGDKLKLEQLDPKQHFTKPPAR
FTEAALVKELEKKGIGRPSTYASIISTIQDRGYVRVESRRFYAEKMGEIVTDRLNQSFDDLMDYDFTARMEGNLDKIAEG
SKDWKEVLNAFFQDFTDDIAKAELDESEGGMSPNNIVETDIKCPTCDRNMGIRTASTGVFLGCSGYALPPKERCKKTINL
GNEEGIINVLEEDVETAALRAKKRCPKCETAMDPYLIDQDRKLHVCGNNPNCDGYIVEKGEFQLKGYDGPIVECDKCGSD
MELKNGRFGKYMDCTSETCKNTRKILRNGDVAPPKEDPVHLPELECEKSDAYFVLRDGASGLFLAASTFPKSRETRAPLV
EELVRFKDRLSPKFVYLTEAPTHDPDGKPAVIRFSRKTKENYVRTETDGKPSGWTALYLDGKWAVTDKRKKPKAEKES

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=614, Percent_Identity=25.2442996742671, Blast_Score=148, Evalue=3e-35,
Organism=Homo sapiens, GI4507635, Length=665, Percent_Identity=25.2631578947368, Blast_Score=136, Evalue=1e-31,
Organism=Escherichia coli, GI1787529, Length=866, Percent_Identity=69.9769053117783, Blast_Score=1284, Evalue=0.0,
Organism=Escherichia coli, GI1788061, Length=570, Percent_Identity=21.9298245614035, Blast_Score=103, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI32563869, Length=526, Percent_Identity=27.1863117870722, Blast_Score=132, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17555378, Length=510, Percent_Identity=24.3137254901961, Blast_Score=112, Evalue=8e-25,
Organism=Saccharomyces cerevisiae, GI6323263, Length=495, Percent_Identity=24.6464646464646, Blast_Score=100, Evalue=7e-22,
Organism=Drosophila melanogaster, GI24585251, Length=629, Percent_Identity=24.9602543720191, Blast_Score=132, Evalue=9e-31,
Organism=Drosophila melanogaster, GI24640096, Length=502, Percent_Identity=25.4980079681275, Blast_Score=122, Evalue=1e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR013498
- InterPro:   IPR005733
- InterPro:   IPR013263
- InterPro:   IPR006171 [H]

Pfam domain/function: PF08272 Topo_Zn_Ribbon; PF01131 Topoisom_bac; PF01751 Toprim; PF01396 zf-C4_Topoisom [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 98512; Mature: 98381

Theoretical pI: Translated: 7.56; Mature: 7.56

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKSLVIVESPAKAKTINKYLGKDFIVKSSVGHVRDLPMGSTSTGKKTAAASTKGMSVED
CCCCEEEEECCCHHHHHHHHHCCCEEEECCCCCHHCCCCCCCCCCCCHHHCCCCCCCCHH
KARIKKERDKKNLINKMGIDPYNDWAANYQILPGKEKVVAELQKLAENADTIYLATDLDR
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCEECCCHHHHHHHHHHHHCCCCEEEEEECCCC
EGEAIAWHLREIIGGDDSRYKRVVFNEITKNAIQQAFETPGELSMPGVNAQQARRFLDRV
CCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHH
VGFMASPLLWKKVARGLSAGRVQSVAVKVLVEREREINAFIPEEFWDIHADTLTSGKENF
HHHHHCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHCCHHHHCCCCCCE
RLQVAQRNGSVFKPLNQADTESAVSILEKAEYEVCKREDRPTKSKPSAPYITSTLQQAAS
EEEEEECCCCEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHH
TRLGYGVKKTMMLAQRLYEGGYITYMRTDSTNLSKEAVETLREFIGSEYGESYLPAAPII
HHHCCCHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEE
YGSKEGAQEAHEAIRPSSVDVKVDDLQGMEADAHKLYNLIWNQFVSCQMTPAQYDSTTLS
ECCCCCHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHCEEECCCCCCCEEEE
VKADEFTLKAKGRILKFDGWTRVQRPMGKNEDQLLPAVQLGDKLKLEQLDPKQHFTKPPA
EEECCEEEEECCCEEEECCCHHHHCCCCCCHHHCCHHHHCCCCCCHHCCCCHHHCCCCCH
RFTEAALVKELEKKGIGRPSTYASIISTIQDRGYVRVESRRFYAEKMGEIVTDRLNQSFD
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHCCHH
DLMDYDFTARMEGNLDKIAEGSKDWKEVLNAFFQDFTDDIAKAELDESEGGMSPNNIVET
HHHCCCCEEEECCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEC
DIKCPTCDRNMGIRTASTGVFLGCSGYALPPKERCKKTINLGNEEGIINVLEEDVETAAL
CCCCCCCCCCCCCEEECCCEEEECCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
RAKKRCPKCETAMDPYLIDQDRKLHVCGNNPNCDGYIVEKGEFQLKGYDGPIVECDKCGS
HHHHCCCCCHHCCCCEEECCCCEEEEECCCCCCCEEEEECCEEEEECCCCCEEECCCCCC
DMELKNGRFGKYMDCTSETCKNTRKILRNGDVAPPKEDPVHLPELECEKSDAYFVLRDGA
CCCCCCCCCCCEECHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCC
SGLFLAASTFPKSRETRAPLVEELVRFKDRLSPKFVYLTEAPTHDPDGKPAVIRFSRKTK
CCEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCEEEEEECHHC
ENYVRTETDGKPSGWTALYLDGKWAVTDKRKKPKAEKES
CCCEECCCCCCCCCEEEEEECCEEEECCCCCCCCCCCCC
>Mature Secondary Structure 
GKSLVIVESPAKAKTINKYLGKDFIVKSSVGHVRDLPMGSTSTGKKTAAASTKGMSVED
CCCEEEEECCCHHHHHHHHHCCCEEEECCCCCHHCCCCCCCCCCCCHHHCCCCCCCCHH
KARIKKERDKKNLINKMGIDPYNDWAANYQILPGKEKVVAELQKLAENADTIYLATDLDR
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCEECCCHHHHHHHHHHHHCCCCEEEEEECCCC
EGEAIAWHLREIIGGDDSRYKRVVFNEITKNAIQQAFETPGELSMPGVNAQQARRFLDRV
CCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHH
VGFMASPLLWKKVARGLSAGRVQSVAVKVLVEREREINAFIPEEFWDIHADTLTSGKENF
HHHHHCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHCCHHHHCCCCCCE
RLQVAQRNGSVFKPLNQADTESAVSILEKAEYEVCKREDRPTKSKPSAPYITSTLQQAAS
EEEEEECCCCEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHH
TRLGYGVKKTMMLAQRLYEGGYITYMRTDSTNLSKEAVETLREFIGSEYGESYLPAAPII
HHHCCCHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEE
YGSKEGAQEAHEAIRPSSVDVKVDDLQGMEADAHKLYNLIWNQFVSCQMTPAQYDSTTLS
ECCCCCHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHCEEECCCCCCCEEEE
VKADEFTLKAKGRILKFDGWTRVQRPMGKNEDQLLPAVQLGDKLKLEQLDPKQHFTKPPA
EEECCEEEEECCCEEEECCCHHHHCCCCCCHHHCCHHHHCCCCCCHHCCCCHHHCCCCCH
RFTEAALVKELEKKGIGRPSTYASIISTIQDRGYVRVESRRFYAEKMGEIVTDRLNQSFD
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHCCHH
DLMDYDFTARMEGNLDKIAEGSKDWKEVLNAFFQDFTDDIAKAELDESEGGMSPNNIVET
HHHCCCCEEEECCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEC
DIKCPTCDRNMGIRTASTGVFLGCSGYALPPKERCKKTINLGNEEGIINVLEEDVETAAL
CCCCCCCCCCCCCEEECCCEEEECCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
RAKKRCPKCETAMDPYLIDQDRKLHVCGNNPNCDGYIVEKGEFQLKGYDGPIVECDKCGS
HHHHCCCCCHHCCCCEEECCCCEEEEECCCCCCCEEEEECCEEEEECCCCCEEECCCCCC
DMELKNGRFGKYMDCTSETCKNTRKILRNGDVAPPKEDPVHLPELECEKSDAYFVLRDGA
CCCCCCCCCCCEECHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCC
SGLFLAASTFPKSRETRAPLVEELVRFKDRLSPKFVYLTEAPTHDPDGKPAVIRFSRKTK
CCEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCEEEEEECHHC
ENYVRTETDGKPSGWTALYLDGKWAVTDKRKKPKAEKES
CCCEECCCCCCCCCEEEEEECCEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]