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The map label for this gene is menF [H]

Identifier: 209694656

GI number: 209694656

Start: 1194026

End: 1195348

Strand: Direct

Name: menF [H]

Synonym: VSAL_I1098

Alternate gene names: 209694656

Gene position: 1194026-1195348 (Clockwise)

Preceding gene: 209694654

Following gene: 209694657

Centisome position: 35.91

GC content: 40.59

Gene sequence:

>1323_bases
TTGTCCACTTTTCAAGATGCCATTGAATCGATTCGTCATCAAATCGCACAAGCAACATCATCTCATACCATTTGTATTGA
CTTAGATTGGACGGTTGGTGATCAACTTATTGATTGGCTTGAAGCGCAACCGATTTACCCTAAGTTTTATTGGCAATCTC
GTGATGGTGAAGAAGAAGTTGCGGTGTTAGGCCAAGTGAAGACCTTTACTGACCCAAAGGCGGCGCAACAAATATTAGCG
CCTCAACAACGAGTGTGGGGCGGGCGATCGTTTGATGGGCGTACTGAACGAAACCGTCGTTGTATGTCTTCTTTTTTCTT
TTTGCCTAAAGTTGAAATTACGCGTTTAAAATCATCGTGGCATTTCATGGTCAATTTGTCAGATGATAATTCTCGTTTAT
TGTCGACGTTAGATAAGTTAAGTGAGCAATTTACGCCATTATCTGAGTTGCATTGCCGTGTTCAACAACGGATCAATACG
CCTGAAAAAGAAGATTGGTCGAACATGGTCAATAAAGCGTTGACTGCCATCTCAAATAAGCAATTTGAAAAGGTGGTATT
AGCACGAAAAACAACACTAACACTTGACCAACCGTTGTCGTCTGCACAATTTTTAAAAGCGAGTCGTAAAGCCAATAGCC
ACAGTTTTCATTTTATGATGGCGTTGGATAGTGAACATTGTTTTATCGGTTCAACCCCTGAGCGTTTATATGTTCGTAAT
GGGTATGCGTTAAAAACAGAAGCGTTAGCGGGAACCATTGGTCGTGGTCATGATGAAGAAGAAGATGATCAACTTGCATC
ATGGTTAATCTCTGACAAGAAAAACCAATATGAAAACCGTCTTGTGGTGGATGATATTGTTAATCGCTTATCTAAATTCA
GTCTTTCAATGAAGGTAGCTGAAAAATCTGAGCTGGTTAAATTACGTAAAGTTCAACATTTAAAACGTCCAATTTCAGCT
GAAATTAATCAAGAATGCAGTGATTCTCAATTGCTTGATAATTTACAGCCAACAGCGGCTATTGCGGGGTTACCTCGCAT
GCCTGCATTGGACTTTATTATTGATAACGAACCTTTTGCTCGTGGTTGGTACAGTGGTGCCGTTGGTTTTTTAAGCCAAC
AACGCAGTGAGTTTTGTGTCGCGATCCGAAGCGCATTGGTTATGGGCAATAAACTTCATCTTTTTGCTGGTGCAGGTATC
GTTCCTGGTTCAGAGCCAAGCAGTGAATGGGATGAATTAGATAGAAAGACATCAACGTTATGCACCTTATTAGAATCTGA
TATTACAATGAATCCAGAAATTACGGAGTCGCAAGTCGCATGA

Upstream 100 bases:

>100_bases
CACTTTTGCTAGAATCACTACATCTAACAAGGAAATAGCAAGTAATTCGGCTTGTGTATAACGGTGCCATGATGGCTGAA
AACGATCAGGAGAGGTTAAT

Downstream 100 bases:

>100_bases
TGCAACCAGAGCAACAAGTTATGCTAAACCAAGTTTGGGCTGAATTAATTATTGAAGAATTGGTTCGAAATGGAGTAAAG
CATGTTTGTATTGCTCCGGG

Product: menaquinone-specific isochorismate synthase

Products: NA

Alternate protein names: Isochorismate mutase [H]

Number of amino acids: Translated: 440; Mature: 439

Protein sequence:

>440_residues
MSTFQDAIESIRHQIAQATSSHTICIDLDWTVGDQLIDWLEAQPIYPKFYWQSRDGEEEVAVLGQVKTFTDPKAAQQILA
PQQRVWGGRSFDGRTERNRRCMSSFFFLPKVEITRLKSSWHFMVNLSDDNSRLLSTLDKLSEQFTPLSELHCRVQQRINT
PEKEDWSNMVNKALTAISNKQFEKVVLARKTTLTLDQPLSSAQFLKASRKANSHSFHFMMALDSEHCFIGSTPERLYVRN
GYALKTEALAGTIGRGHDEEEDDQLASWLISDKKNQYENRLVVDDIVNRLSKFSLSMKVAEKSELVKLRKVQHLKRPISA
EINQECSDSQLLDNLQPTAAIAGLPRMPALDFIIDNEPFARGWYSGAVGFLSQQRSEFCVAIRSALVMGNKLHLFAGAGI
VPGSEPSSEWDELDRKTSTLCTLLESDITMNPEITESQVA

Sequences:

>Translated_440_residues
MSTFQDAIESIRHQIAQATSSHTICIDLDWTVGDQLIDWLEAQPIYPKFYWQSRDGEEEVAVLGQVKTFTDPKAAQQILA
PQQRVWGGRSFDGRTERNRRCMSSFFFLPKVEITRLKSSWHFMVNLSDDNSRLLSTLDKLSEQFTPLSELHCRVQQRINT
PEKEDWSNMVNKALTAISNKQFEKVVLARKTTLTLDQPLSSAQFLKASRKANSHSFHFMMALDSEHCFIGSTPERLYVRN
GYALKTEALAGTIGRGHDEEEDDQLASWLISDKKNQYENRLVVDDIVNRLSKFSLSMKVAEKSELVKLRKVQHLKRPISA
EINQECSDSQLLDNLQPTAAIAGLPRMPALDFIIDNEPFARGWYSGAVGFLSQQRSEFCVAIRSALVMGNKLHLFAGAGI
VPGSEPSSEWDELDRKTSTLCTLLESDITMNPEITESQVA
>Mature_439_residues
STFQDAIESIRHQIAQATSSHTICIDLDWTVGDQLIDWLEAQPIYPKFYWQSRDGEEEVAVLGQVKTFTDPKAAQQILAP
QQRVWGGRSFDGRTERNRRCMSSFFFLPKVEITRLKSSWHFMVNLSDDNSRLLSTLDKLSEQFTPLSELHCRVQQRINTP
EKEDWSNMVNKALTAISNKQFEKVVLARKTTLTLDQPLSSAQFLKASRKANSHSFHFMMALDSEHCFIGSTPERLYVRNG
YALKTEALAGTIGRGHDEEEDDQLASWLISDKKNQYENRLVVDDIVNRLSKFSLSMKVAEKSELVKLRKVQHLKRPISAE
INQECSDSQLLDNLQPTAAIAGLPRMPALDFIIDNEPFARGWYSGAVGFLSQQRSEFCVAIRSALVMGNKLHLFAGAGIV
PGSEPSSEWDELDRKTSTLCTLLESDITMNPEITESQVA

Specific function: Menaquinone biosynthesis. [C]

COG id: COG1169

COG function: function code HQ; Isochorismate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isochorismate synthase family [H]

Homologues:

Organism=Escherichia coli, GI87082077, Length=443, Percent_Identity=39.2776523702032, Blast_Score=299, Evalue=3e-82,
Organism=Escherichia coli, GI1786809, Length=272, Percent_Identity=31.6176470588235, Blast_Score=137, Evalue=2e-33,
Organism=Escherichia coli, GI1787518, Length=323, Percent_Identity=27.2445820433437, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI1788114, Length=224, Percent_Identity=27.6785714285714, Blast_Score=89, Evalue=7e-19,
Organism=Saccharomyces cerevisiae, GI6320935, Length=276, Percent_Identity=27.536231884058, Blast_Score=82, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005801
- InterPro:   IPR015890
- InterPro:   IPR004561 [H]

Pfam domain/function: PF00425 Chorismate_bind [H]

EC number: =5.4.4.2 [H]

Molecular weight: Translated: 49809; Mature: 49678

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTFQDAIESIRHQIAQATSSHTICIDLDWTVGDQLIDWLEAQPIYPKFYWQSRDGEEEV
CCHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHCCCCCCEEEECCCCCHHHH
AVLGQVKTFTDPKAAQQILAPQQRVWGGRSFDGRTERNRRCMSSFFFLPKVEITRLKSSW
HHHHHHHCCCCHHHHHHHHCCHHHHCCCCCCCCCCHHHHHHHHHHHCCCCHHHEEECCCE
HFMVNLSDDNSRLLSTLDKLSEQFTPLSELHCRVQQRINTPEKEDWSNMVNKALTAISNK
EEEEEECCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCH
QFEKVVLARKTTLTLDQPLSSAQFLKASRKANSHSFHFMMALDSEHCFIGSTPERLYVRN
HHHHHHHHHHHEEEECCCCCHHHHHHHHHCCCCCCEEEEEEECCCCEEECCCCCEEEEEC
GYALKTEALAGTIGRGHDEEEDDQLASWLISDKKNQYENRLVVDDIVNRLSKFSLSMKVA
CCEEEHHHHHHHCCCCCCCCHHHHHHHHHHHCCCHHHHCCEEHHHHHHHHHHHHHHHHHH
EKSELVKLRKVQHLKRPISAEINQECSDSQLLDNLQPTAAIAGLPRMPALDFIIDNEPFA
HHHHHHHHHHHHHHHCCCCHHHCCCCCHHHHHHCCCCHHHHHCCCCCCCEEEEECCCCHH
RGWYSGAVGFLSQQRSEFCVAIRSALVMGNKLHLFAGAGIVPGSEPSSEWDELDRKTSTL
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHH
CTLLESDITMNPEITESQVA
HHHHHCCCCCCCCCCHHCCC
>Mature Secondary Structure 
STFQDAIESIRHQIAQATSSHTICIDLDWTVGDQLIDWLEAQPIYPKFYWQSRDGEEEV
CHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHCCCCCCEEEECCCCCHHHH
AVLGQVKTFTDPKAAQQILAPQQRVWGGRSFDGRTERNRRCMSSFFFLPKVEITRLKSSW
HHHHHHHCCCCHHHHHHHHCCHHHHCCCCCCCCCCHHHHHHHHHHHCCCCHHHEEECCCE
HFMVNLSDDNSRLLSTLDKLSEQFTPLSELHCRVQQRINTPEKEDWSNMVNKALTAISNK
EEEEEECCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCH
QFEKVVLARKTTLTLDQPLSSAQFLKASRKANSHSFHFMMALDSEHCFIGSTPERLYVRN
HHHHHHHHHHHEEEECCCCCHHHHHHHHHCCCCCCEEEEEEECCCCEEECCCCCEEEEEC
GYALKTEALAGTIGRGHDEEEDDQLASWLISDKKNQYENRLVVDDIVNRLSKFSLSMKVA
CCEEEHHHHHHHCCCCCCCCHHHHHHHHHHHCCCHHHHCCEEHHHHHHHHHHHHHHHHHH
EKSELVKLRKVQHLKRPISAEINQECSDSQLLDNLQPTAAIAGLPRMPALDFIIDNEPFA
HHHHHHHHHHHHHHHCCCCHHHCCCCCHHHHHHCCCCHHHHHCCCCCCCEEEEECCCCHH
RGWYSGAVGFLSQQRSEFCVAIRSALVMGNKLHLFAGAGIVPGSEPSSEWDELDRKTSTL
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHH
CTLLESDITMNPEITESQVA
HHHHHCCCCCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8549818; 8764478; 9205837; 9278503; 2666397; 7984428 [H]