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The map label for this gene is purF [H]

Identifier: 209694639

GI number: 209694639

Start: 1175118

End: 1176632

Strand: Direct

Name: purF [H]

Synonym: VSAL_I1080

Alternate gene names: 209694639

Gene position: 1175118-1176632 (Clockwise)

Preceding gene: 209694638

Following gene: 209694641

Centisome position: 35.34

GC content: 41.45

Gene sequence:

>1515_bases
ATGTGCGGTATTGTTGGAATCGTAGGCGCAACACCTGTAAACCAGTTAATCTATGACGCTTTGACTGTGTTACAACATCG
TGGCCAAGACGCTGCTGGTATTATTACCGTAGAAAGCAATCGTTTCCGTCTGAGAAAGGCGAACGGTTTGGTTAAAGATG
TATTTGAAGCAAAACACATGCAACGCCTAAAAGGTAACGTTGGTATCGGCCATGTTCGTTATCCTACTGCAGGCAGTTCT
AGTGCTTCTGAAGCTCAACCTTTCTATGTTAACTCACCATTTGGGATCACATTGGCGCACAATGGTAATCTAACCAATGC
GAATGAAATCCGTGAGACCTTATTTGAACAAGAACGTCGTCATGTTAATACAACGTCAGATTCAGAAGTATTATTAAATA
TTTTTGCTCATCATCTTGATTCAACCACTCATTACCCTCTTACTGCTGATGACATTTTTAAAACCGTCGCTAACGTGCAT
GAGCAAATTCGTGGTGCGTATGCGGTAACTGCAATGATCATCGGGCATGGTATGGTTGCATTCCGTGATCCAAATGGCAT
TCGTCCTTTGTGTTTAGGGAAGCGTGACGTTGAAGGTCGTACCGAGTACATGGTTGCGTCTGAATCTGTTGCTTTGGATG
CGGTTGGTTTTGATTTTGTTCGTGATGTCGCTCCTGGCGAAGCCATTTATGCGCCATTTGAAGGCGGATTGTATACACAA
CAATGTGCGCACGATCCAAAGCTGAATCCATGCATTTTCGAGTTTGTGTATTTTGCTCGTCCAGATTCTTTTATTGATAA
AATTTCGGTCTATAGCGCACGTCTTGAAATGGGCAAACGCTTAGGTGATAAAATTAAACGTGAGTGGGATGATCTCGACA
TTGATGTGGTTATTCCGATCCCTGAAACATCGTGTGATATTGCACTAGAAATCGCTCAAATTATTAATAAACCATACCGC
CAGGGGTTTGTGAAAAACCGTTATGTTGGTCGTACGTTTATTATGCCAGGTCAGCAAGAACGTAAAAAATCAGTACGTCG
AAAATTGAATGCAATCCGTTCTGAATTTAAAGATAAAAATGTACTGCTAGTCGACGATTCAATTGTTCGTGGCACAACAT
CAGAACAAATTATCCAAATGGCTCGTGATTCGGGTGCTAAAAATGTTTACATGGTGTCAGCAGCACCTGAGATTCGTTTC
CCTAACGTATACGGTATTGATATGCCGAGTGCAAACGAGTTGATTGCTCATGGTCGTGAAGTCGATGAAATCTGCCAGAA
AATTGGTGCAGATGCATTAATTTTCCAAGATTTATCTGATTTAGTTGATGCGGTTGGTTGTGGTAACCGAGAAGTTGAAC
AATTTGAAACATCGGTATTTAATGCACACTATGTTACCGGTGATGTAGATCAAAACTACCTAGAGTACCTCGAAAGTTTA
CGCGGTGATGATGCTAAGCAAGAAAGCGAAGCTCAAGATGTGGCAAGTTTAGATATTTACAATGAAGGCGTTTAA

Upstream 100 bases:

>100_bases
GTAGTAATTGAATGGTTCTTTGATTACTTAAAAGATACATCAAGTTTTCTTCCTGGCGCTAAATAGCGCCAGTTTTTTAT
CAAATAAAGGACTCTAGGAC

Downstream 100 bases:

>100_bases
ATTCGTTTAGCAATGAGCGTATTTTAATTACGTTACGCCTATAAAGTTAAAAAAGCCTGTGATGTATTCACAGGCTTTTT
TGTTATTTTGGAATATGATT

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 504; Mature: 504

Protein sequence:

>504_residues
MCGIVGIVGATPVNQLIYDALTVLQHRGQDAAGIITVESNRFRLRKANGLVKDVFEAKHMQRLKGNVGIGHVRYPTAGSS
SASEAQPFYVNSPFGITLAHNGNLTNANEIRETLFEQERRHVNTTSDSEVLLNIFAHHLDSTTHYPLTADDIFKTVANVH
EQIRGAYAVTAMIIGHGMVAFRDPNGIRPLCLGKRDVEGRTEYMVASESVALDAVGFDFVRDVAPGEAIYAPFEGGLYTQ
QCAHDPKLNPCIFEFVYFARPDSFIDKISVYSARLEMGKRLGDKIKREWDDLDIDVVIPIPETSCDIALEIAQIINKPYR
QGFVKNRYVGRTFIMPGQQERKKSVRRKLNAIRSEFKDKNVLLVDDSIVRGTTSEQIIQMARDSGAKNVYMVSAAPEIRF
PNVYGIDMPSANELIAHGREVDEICQKIGADALIFQDLSDLVDAVGCGNREVEQFETSVFNAHYVTGDVDQNYLEYLESL
RGDDAKQESEAQDVASLDIYNEGV

Sequences:

>Translated_504_residues
MCGIVGIVGATPVNQLIYDALTVLQHRGQDAAGIITVESNRFRLRKANGLVKDVFEAKHMQRLKGNVGIGHVRYPTAGSS
SASEAQPFYVNSPFGITLAHNGNLTNANEIRETLFEQERRHVNTTSDSEVLLNIFAHHLDSTTHYPLTADDIFKTVANVH
EQIRGAYAVTAMIIGHGMVAFRDPNGIRPLCLGKRDVEGRTEYMVASESVALDAVGFDFVRDVAPGEAIYAPFEGGLYTQ
QCAHDPKLNPCIFEFVYFARPDSFIDKISVYSARLEMGKRLGDKIKREWDDLDIDVVIPIPETSCDIALEIAQIINKPYR
QGFVKNRYVGRTFIMPGQQERKKSVRRKLNAIRSEFKDKNVLLVDDSIVRGTTSEQIIQMARDSGAKNVYMVSAAPEIRF
PNVYGIDMPSANELIAHGREVDEICQKIGADALIFQDLSDLVDAVGCGNREVEQFETSVFNAHYVTGDVDQNYLEYLESL
RGDDAKQESEAQDVASLDIYNEGV
>Mature_504_residues
MCGIVGIVGATPVNQLIYDALTVLQHRGQDAAGIITVESNRFRLRKANGLVKDVFEAKHMQRLKGNVGIGHVRYPTAGSS
SASEAQPFYVNSPFGITLAHNGNLTNANEIRETLFEQERRHVNTTSDSEVLLNIFAHHLDSTTHYPLTADDIFKTVANVH
EQIRGAYAVTAMIIGHGMVAFRDPNGIRPLCLGKRDVEGRTEYMVASESVALDAVGFDFVRDVAPGEAIYAPFEGGLYTQ
QCAHDPKLNPCIFEFVYFARPDSFIDKISVYSARLEMGKRLGDKIKREWDDLDIDVVIPIPETSCDIALEIAQIINKPYR
QGFVKNRYVGRTFIMPGQQERKKSVRRKLNAIRSEFKDKNVLLVDDSIVRGTTSEQIIQMARDSGAKNVYMVSAAPEIRF
PNVYGIDMPSANELIAHGREVDEICQKIGADALIFQDLSDLVDAVGCGNREVEQFETSVFNAHYVTGDVDQNYLEYLESL
RGDDAKQESEAQDVASLDIYNEGV

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=474, Percent_Identity=36.2869198312236, Blast_Score=269, Evalue=5e-72,
Organism=Escherichia coli, GI1788651, Length=505, Percent_Identity=77.4257425742574, Blast_Score=813, Evalue=0.0,
Organism=Escherichia coli, GI1790167, Length=222, Percent_Identity=30.1801801801802, Blast_Score=76, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17554892, Length=470, Percent_Identity=34.468085106383, Blast_Score=246, Evalue=2e-65,
Organism=Saccharomyces cerevisiae, GI6323958, Length=509, Percent_Identity=49.705304518664, Blast_Score=486, Evalue=1e-138,
Organism=Drosophila melanogaster, GI24659598, Length=483, Percent_Identity=34.5755693581781, Blast_Score=257, Evalue=1e-68,
Organism=Drosophila melanogaster, GI24659604, Length=480, Percent_Identity=34.5833333333333, Blast_Score=254, Evalue=1e-67,
Organism=Drosophila melanogaster, GI28573187, Length=487, Percent_Identity=33.4702258726899, Blast_Score=242, Evalue=3e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 56062; Mature: 56062

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGIVGIVGATPVNQLIYDALTVLQHRGQDAAGIITVESNRFRLRKANGLVKDVFEAKHM
CCCEEEECCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCEEEEHHCCHHHHHHHHHHH
QRLKGNVGIGHVRYPTAGSSSASEAQPFYVNSPFGITLAHNGNLTNANEIRETLFEQERR
HHHCCCCCCCEEECCCCCCCCCCCCCCEEECCCCEEEEEECCCCCCHHHHHHHHHHHHHH
HVNTTSDSEVLLNIFAHHLDSTTHYPLTADDIFKTVANVHEQIRGAYAVTAMIIGHGMVA
CCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEE
FRDPNGIRPLCLGKRDVEGRTEYMVASESVALDAVGFDFVRDVAPGEAIYAPFEGGLYTQ
EECCCCCCCCCCCCCCCCCCHHEEEECCCEEEEHHHHHHHHHCCCCCEEECCCCCCCCHH
QCAHDPKLNPCIFEFVYFARPDSFIDKISVYSARLEMGKRLGDKIKREWDDLDIDVVIPI
HHCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEC
PETSCDIALEIAQIINKPYRQGFVKNRYVGRTFIMPGQQERKKSVRRKLNAIRSEFKDKN
CCCCHHHHHHHHHHHCCHHHCCHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCC
VLLVDDSIVRGTTSEQIIQMARDSGAKNVYMVSAAPEIRFPNVYGIDMPSANELIAHGRE
EEEECCCHHCCCCHHHHHHHHHCCCCCEEEEEECCCCCCCCCEEECCCCCHHHHHHCCCC
VDEICQKIGADALIFQDLSDLVDAVGCGNREVEQFETSVFNAHYVTGDVDQNYLEYLESL
HHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHEEEEEECCCCHHHHHHHHHH
RGDDAKQESEAQDVASLDIYNEGV
CCCCCHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MCGIVGIVGATPVNQLIYDALTVLQHRGQDAAGIITVESNRFRLRKANGLVKDVFEAKHM
CCCEEEECCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCEEEEHHCCHHHHHHHHHHH
QRLKGNVGIGHVRYPTAGSSSASEAQPFYVNSPFGITLAHNGNLTNANEIRETLFEQERR
HHHCCCCCCCEEECCCCCCCCCCCCCCEEECCCCEEEEEECCCCCCHHHHHHHHHHHHHH
HVNTTSDSEVLLNIFAHHLDSTTHYPLTADDIFKTVANVHEQIRGAYAVTAMIIGHGMVA
CCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEE
FRDPNGIRPLCLGKRDVEGRTEYMVASESVALDAVGFDFVRDVAPGEAIYAPFEGGLYTQ
EECCCCCCCCCCCCCCCCCCHHEEEECCCEEEEHHHHHHHHHCCCCCEEECCCCCCCCHH
QCAHDPKLNPCIFEFVYFARPDSFIDKISVYSARLEMGKRLGDKIKREWDDLDIDVVIPI
HHCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEC
PETSCDIALEIAQIINKPYRQGFVKNRYVGRTFIMPGQQERKKSVRRKLNAIRSEFKDKN
CCCCHHHHHHHHHHHCCHHHCCHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCC
VLLVDDSIVRGTTSEQIIQMARDSGAKNVYMVSAAPEIRFPNVYGIDMPSANELIAHGRE
EEEECCCHHCCCCHHHHHHHHHCCCCCEEEEEECCCCCCCCCEEECCCCCHHHHHHCCCC
VDEICQKIGADALIFQDLSDLVDAVGCGNREVEQFETSVFNAHYVTGDVDQNYLEYLESL
HHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHEEEEEECCCCHHHHHHHHHH
RGDDAKQESEAQDVASLDIYNEGV
CCCCCHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3047685; 6443594; 6277938; 9205837; 9278503; 3040734; 7037784; 9333323; 9514258 [H]