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The map label for this gene is folC [H]

Identifier: 209694636

GI number: 209694636

Start: 1172671

End: 1173951

Strand: Direct

Name: folC [H]

Synonym: VSAL_I1077

Alternate gene names: 209694636

Gene position: 1172671-1173951 (Clockwise)

Preceding gene: 209694635

Following gene: 209694637

Centisome position: 35.27

GC content: 40.91

Gene sequence:

>1281_bases
ATGACAACATTTGATTCTCCGACAGCCACATCTGATTTATCAGTGTGGCTTCGTTATTTAGAAAGTATTCATACTAGTGC
GATTGATTTAGGCCTTGACCGTGTTCAGCGTGTCGCACAGAGAGCAAAATTGACAAAACCTGCCCCTACAGTGATTACTG
TTGCGGGAACCAATGGTAAAGGATCTACCTGTGCGTTAATGGAATCTATTTTACTCAATGCAGGTTACCGTGTTGGTGTT
TATAGTTCCCCTCATCTTATTCGTTACAATGAACGTATTCGTATCAATGGTAATGATCTATCTGATTCGCTTATCTCTCA
ATCTTTTGCTGCTATTGAAGCGTGGCGTAAAGACACACCATTAAGCCTTTTTGAATTTGGTACCTTAGCTGCGTTATGGG
CTTTTAAAGCGCATCAAGTGGATGTGCTATTGCTTGAAGTCGGTCTTGGTGGCCGTTTAGATGCAACCAATGTTGTTGAT
CATGATGTGTCTATTATCACAAGTTTAGCGGTTGATCATGTTGATTGGCTTGGTGATGATATTAATGTGATCGGTTTTGA
AAAAGCGGGGATATTCCGTTCAGACAAACCCGCAATATGTGGTCAACCTAATCCACCATCGAGCGTTGCTGCCCATGCAG
ATGATATTAATGCAGATTTGCATCAAGTAGGATATCAATTTACTTATAAGGTAAATGAAGATAATAAAACATGGTCTTGG
CAGAGTGGGGCATTTGACTTAACGGGTCTTCCTCTACCTAAATTACCGCTTGCTAATGCGGCAACGGCAATTATGGCGCT
TGGCTGTGCTGATTTAGAATTAACGGATGTTAATATTGTCGAAGGGTTGAAAGCCGCAAGTTTACCGGGTCGTATGCAAA
TACTAACCCGTGATGAAGAGAGTATTGAGCCACTTATTCTTTTAGATGTCGCACATAATCCACATTCTGCTCATTATCTA
GTACAACAAGTAAGACTGCGTTACCCAAATAAGAAAATTCATGCAGTAGTTGGTATGCTACATGATAAAGACATTGAATC
GACAGTGAATGAGTTCACGGGTGTTGTTGATGTTTGGTATCCTGCTTCTCTTTCTGGGCCCCGTGCCGCGAAAGCAAGTG
AACTTGTTGCATTTATAGAGCAAGAAACAACAGAATACAGTTCACCAGTTGAGGCATTTAAAGTTGCACTTCAACACGCT
GGTGATGATGAATTAATTTTAGTTTTTGGTTCTTTCCATACGGTTGGTGAAGTATGGAATTACTGGAAACAAGGATCGTG
A

Upstream 100 bases:

>100_bases
AAATCAAATGTAAATTGTCTTAGGGGGTTATTTCTTTCTTTAAATAAGTACCTTAAGTCAATATACTGTAACTACGTTCC
GTCAGTGAAGTAGTTGAACA

Downstream 100 bases:

>100_bases
GGTATATGAGTGTCTAGTCCATTTCAACGACGTTTAGTTGGCACCATTATTTTGGTTGCTATTGGAGTTATTGTATTACC
CGACTTATTTGATGGTAAGA

Product: FolC bifunctional protein (tetrahydrofolate synthase) (folylpolyglutamate synthase)

Products: NA

Alternate protein names: Folylpolyglutamate synthase; Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolylpolyglutamate synthase; Tetrahydrofolate synthase; Dihydrofolate synthase [H]

Number of amino acids: Translated: 426; Mature: 425

Protein sequence:

>426_residues
MTTFDSPTATSDLSVWLRYLESIHTSAIDLGLDRVQRVAQRAKLTKPAPTVITVAGTNGKGSTCALMESILLNAGYRVGV
YSSPHLIRYNERIRINGNDLSDSLISQSFAAIEAWRKDTPLSLFEFGTLAALWAFKAHQVDVLLLEVGLGGRLDATNVVD
HDVSIITSLAVDHVDWLGDDINVIGFEKAGIFRSDKPAICGQPNPPSSVAAHADDINADLHQVGYQFTYKVNEDNKTWSW
QSGAFDLTGLPLPKLPLANAATAIMALGCADLELTDVNIVEGLKAASLPGRMQILTRDEESIEPLILLDVAHNPHSAHYL
VQQVRLRYPNKKIHAVVGMLHDKDIESTVNEFTGVVDVWYPASLSGPRAAKASELVAFIEQETTEYSSPVEAFKVALQHA
GDDELILVFGSFHTVGEVWNYWKQGS

Sequences:

>Translated_426_residues
MTTFDSPTATSDLSVWLRYLESIHTSAIDLGLDRVQRVAQRAKLTKPAPTVITVAGTNGKGSTCALMESILLNAGYRVGV
YSSPHLIRYNERIRINGNDLSDSLISQSFAAIEAWRKDTPLSLFEFGTLAALWAFKAHQVDVLLLEVGLGGRLDATNVVD
HDVSIITSLAVDHVDWLGDDINVIGFEKAGIFRSDKPAICGQPNPPSSVAAHADDINADLHQVGYQFTYKVNEDNKTWSW
QSGAFDLTGLPLPKLPLANAATAIMALGCADLELTDVNIVEGLKAASLPGRMQILTRDEESIEPLILLDVAHNPHSAHYL
VQQVRLRYPNKKIHAVVGMLHDKDIESTVNEFTGVVDVWYPASLSGPRAAKASELVAFIEQETTEYSSPVEAFKVALQHA
GDDELILVFGSFHTVGEVWNYWKQGS
>Mature_425_residues
TTFDSPTATSDLSVWLRYLESIHTSAIDLGLDRVQRVAQRAKLTKPAPTVITVAGTNGKGSTCALMESILLNAGYRVGVY
SSPHLIRYNERIRINGNDLSDSLISQSFAAIEAWRKDTPLSLFEFGTLAALWAFKAHQVDVLLLEVGLGGRLDATNVVDH
DVSIITSLAVDHVDWLGDDINVIGFEKAGIFRSDKPAICGQPNPPSSVAAHADDINADLHQVGYQFTYKVNEDNKTWSWQ
SGAFDLTGLPLPKLPLANAATAIMALGCADLELTDVNIVEGLKAASLPGRMQILTRDEESIEPLILLDVAHNPHSAHYLV
QQVRLRYPNKKIHAVVGMLHDKDIESTVNEFTGVVDVWYPASLSGPRAAKASELVAFIEQETTEYSSPVEAFKVALQHAG
DDELILVFGSFHTVGEVWNYWKQGS

Specific function: Conversion of folates to polyglutamate derivatives [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932990, Length=302, Percent_Identity=32.7814569536424, Blast_Score=125, Evalue=9e-29,
Organism=Homo sapiens, GI66932984, Length=302, Percent_Identity=32.7814569536424, Blast_Score=125, Evalue=9e-29,
Organism=Escherichia coli, GI1788654, Length=418, Percent_Identity=49.7607655502392, Blast_Score=394, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI17553148, Length=474, Percent_Identity=28.9029535864979, Blast_Score=143, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI71984923, Length=468, Percent_Identity=29.0598290598291, Blast_Score=142, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI17553150, Length=468, Percent_Identity=29.0598290598291, Blast_Score=142, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6323760, Length=421, Percent_Identity=28.5035629453682, Blast_Score=129, Evalue=7e-31,
Organism=Saccharomyces cerevisiae, GI6324815, Length=180, Percent_Identity=35.5555555555556, Blast_Score=104, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24641571, Length=186, Percent_Identity=40.3225806451613, Blast_Score=119, Evalue=6e-27,
Organism=Drosophila melanogaster, GI24581568, Length=149, Percent_Identity=42.9530201342282, Blast_Score=102, Evalue=5e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17; =6.3.2.12 [H]

Molecular weight: Translated: 46556; Mature: 46425

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS01011 FOLYLPOLYGLU_SYNT_1 ; PS01012 FOLYLPOLYGLU_SYNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTFDSPTATSDLSVWLRYLESIHTSAIDLGLDRVQRVAQRAKLTKPAPTVITVAGTNGK
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCC
GSTCALMESILLNAGYRVGVYSSPHLIRYNERIRINGNDLSDSLISQSFAAIEAWRKDTP
CCHHHHHHHHHHCCCCEEEECCCCCEEEECCEEEECCCCHHHHHHHHHHHHHHHHCCCCC
LSLFEFGTLAALWAFKAHQVDVLLLEVGLGGRLDATNVVDHDVSIITSLAVDHVDWLGDD
CHHHHHHHHHHHHHHHHCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
INVIGFEKAGIFRSDKPAICGQPNPPSSVAAHADDINADLHQVGYQFTYKVNEDNKTWSW
CEEEEEECCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCHHHHCCEEEEEEECCCCCEEEE
QSGAFDLTGLPLPKLPLANAATAIMALGCADLELTDVNIVEGLKAASLPGRMQILTRDEE
CCCCEEECCCCCCCCCCHHHHHHHHHHCCCCCEEECCHHHHCCHHCCCCCCEEEEECCCC
SIEPLILLDVAHNPHSAHYLVQQVRLRYPNKKIHAVVGMLHDKDIESTVNEFTGVVDVWY
CCCCEEEEEECCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHCCEEEEEE
PASLSGPRAAKASELVAFIEQETTEYSSPVEAFKVALQHAGDDELILVFGSFHTVGEVWN
CCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEEEECHHHHHHHHH
YWKQGS
HHCCCC
>Mature Secondary Structure 
TTFDSPTATSDLSVWLRYLESIHTSAIDLGLDRVQRVAQRAKLTKPAPTVITVAGTNGK
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCC
GSTCALMESILLNAGYRVGVYSSPHLIRYNERIRINGNDLSDSLISQSFAAIEAWRKDTP
CCHHHHHHHHHHCCCCEEEECCCCCEEEECCEEEECCCCHHHHHHHHHHHHHHHHCCCCC
LSLFEFGTLAALWAFKAHQVDVLLLEVGLGGRLDATNVVDHDVSIITSLAVDHVDWLGDD
CHHHHHHHHHHHHHHHHCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
INVIGFEKAGIFRSDKPAICGQPNPPSSVAAHADDINADLHQVGYQFTYKVNEDNKTWSW
CEEEEEECCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCHHHHCCEEEEEEECCCCCEEEE
QSGAFDLTGLPLPKLPLANAATAIMALGCADLELTDVNIVEGLKAASLPGRMQILTRDEE
CCCCEEECCCCCCCCCCHHHHHHHHHHCCCCCEEECCHHHHCCHHCCCCCCEEEEECCCC
SIEPLILLDVAHNPHSAHYLVQQVRLRYPNKKIHAVVGMLHDKDIESTVNEFTGVVDVWY
CCCCEEEEEECCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHCCEEEEEE
PASLSGPRAAKASELVAFIEQETTEYSSPVEAFKVALQHAGDDELILVFGSFHTVGEVWN
CCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEEEECHHHHHHHHH
YWKQGS
HHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3040739; 3040734; 9205837; 9278503; 1989505 [H]