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The map label for this gene is rlmG

Identifier: 209694514

GI number: 209694514

Start: 1044383

End: 1045531

Strand: Reverse

Name: rlmG

Synonym: VSAL_I0947

Alternate gene names: 209694514

Gene position: 1045531-1044383 (Counterclockwise)

Preceding gene: 209694526

Following gene: 209694513

Centisome position: 31.44

GC content: 38.03

Gene sequence:

>1149_bases
ATGAAAACTGAATTATGTTTGTTTGATCGTACTCTCAATCTACAGCGTTACCCAAAACGTGCTCAAGAATTATTACAAGC
ATGGGATGCCGGTGATGAGTTCATCATTAAGCATGTAGAAGAAGAGCTAAACCTAGAAGACAATAAAAACATCCTTATCT
TAAATGATAATTTTGGTGCTTTATCTTGCTGGTTCTCTGAAAAGCACAATGTCACCATGATGACCGATTCGTTTGTTTCT
CAACGAGGTACATTAAAAAACCTACAACGTAATCAATGTAACCGCGTACAGTTGATCTCTTCAACGGAGGAAATGCCTGA
AGGTTTCGACCTTGTGATAATGCAAATCCCAAAAAACAATCGAATGCTCGCTTGGCAACTTCAACAGCTTCGCCAATCAA
TGACGGCTGAATGCCCAATTATTGCCGTAAATAAAGCGAAAGAAATACACAGCTCAACACTTGAAGTTTTTGAAGACTAT
TTAGGGGAAACCAAGACATCATTAGCTTGGAAAAAACACCGCCTAGTTTTCTCAAATGCAAATGCAACCAATCCACTAAC
CATTGCAGAAGCCGTGTGTTGGAGTGTTGATAATGAAGATATCGATCTGCTAAATTACCCTAATGTGTATTCAGGTGAGC
GTTTAGATCAAGGCGCTCGTTTTATGCTTGAACATATTCCAGTTGATGCAGAATTGCGTCATATCATCGACTTAGGTTGT
GGCAATGGTGTTTTGAGTGTTAAAGCTGCTCAACTAAATCCAGAAGCACGAATTACCTGCATAGATGAAAGCTTTATGGC
TGTAGAATCAGCTCGTCGTAATTTAGAAGTCAATCTAGGCAAAGAACGTCAATTTCAGTTTATTGCGAATAACTGTTTGG
ATGGATTTAAAAAACACAGCAGTTACTTAGTATTATGTAATCCTCCTTTTCATCAAGGCCAAGCCGTTACTGACCATATT
GCATGGCAAATGTTCTGTGATGCTAAGCACATCTTATGCAAAGAAGGGAAATTATTAGTTATTGGAAACCGTCATCTTGA
TTATGATGATAAATTATGTCGCTTGTTCGGTGAAGAAAACGTCACCACCATTGCTAGCAATTCCAAATTCGTTATTTTAG
AAGCCGTTAAAGCTGAAAAATCAAAATAA

Upstream 100 bases:

>100_bases
GAATCATATAAATCCCTTCTTTATTTCAATTCTTCTATTTCCACTTTATACACCATTTGCGACAATAGCCAGAAGCGTTC
CTTATTTGAAGATCCTATAT

Downstream 100 bases:

>100_bases
AAAGGATTCCCCAATGAAAAAGGCCATTATTGCCCTTTCTGCTCTATTTTTAGCTGCATGTAGTGCTCCTTCTGATCCTC
AGTTGGATATCCGACCTGTT

Product: methyltransferase

Products: NA

Alternate protein names: 23S rRNA m2G1835 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase rlmG

Number of amino acids: Translated: 382; Mature: 382

Protein sequence:

>382_residues
MKTELCLFDRTLNLQRYPKRAQELLQAWDAGDEFIIKHVEEELNLEDNKNILILNDNFGALSCWFSEKHNVTMMTDSFVS
QRGTLKNLQRNQCNRVQLISSTEEMPEGFDLVIMQIPKNNRMLAWQLQQLRQSMTAECPIIAVNKAKEIHSSTLEVFEDY
LGETKTSLAWKKHRLVFSNANATNPLTIAEAVCWSVDNEDIDLLNYPNVYSGERLDQGARFMLEHIPVDAELRHIIDLGC
GNGVLSVKAAQLNPEARITCIDESFMAVESARRNLEVNLGKERQFQFIANNCLDGFKKHSSYLVLCNPPFHQGQAVTDHI
AWQMFCDAKHILCKEGKLLVIGNRHLDYDDKLCRLFGEENVTTIASNSKFVILEAVKAEKSK

Sequences:

>Translated_382_residues
MKTELCLFDRTLNLQRYPKRAQELLQAWDAGDEFIIKHVEEELNLEDNKNILILNDNFGALSCWFSEKHNVTMMTDSFVS
QRGTLKNLQRNQCNRVQLISSTEEMPEGFDLVIMQIPKNNRMLAWQLQQLRQSMTAECPIIAVNKAKEIHSSTLEVFEDY
LGETKTSLAWKKHRLVFSNANATNPLTIAEAVCWSVDNEDIDLLNYPNVYSGERLDQGARFMLEHIPVDAELRHIIDLGC
GNGVLSVKAAQLNPEARITCIDESFMAVESARRNLEVNLGKERQFQFIANNCLDGFKKHSSYLVLCNPPFHQGQAVTDHI
AWQMFCDAKHILCKEGKLLVIGNRHLDYDDKLCRLFGEENVTTIASNSKFVILEAVKAEKSK
>Mature_382_residues
MKTELCLFDRTLNLQRYPKRAQELLQAWDAGDEFIIKHVEEELNLEDNKNILILNDNFGALSCWFSEKHNVTMMTDSFVS
QRGTLKNLQRNQCNRVQLISSTEEMPEGFDLVIMQIPKNNRMLAWQLQQLRQSMTAECPIIAVNKAKEIHSSTLEVFEDY
LGETKTSLAWKKHRLVFSNANATNPLTIAEAVCWSVDNEDIDLLNYPNVYSGERLDQGARFMLEHIPVDAELRHIIDLGC
GNGVLSVKAAQLNPEARITCIDESFMAVESARRNLEVNLGKERQFQFIANNCLDGFKKHSSYLVLCNPPFHQGQAVTDHI
AWQMFCDAKHILCKEGKLLVIGNRHLDYDDKLCRLFGEENVTTIASNSKFVILEAVKAEKSK

Specific function: Specifically methylates the guanosine in position 1835 (m2G1835) of 23S rRNA

COG id: COG2813

COG function: function code J; 16S RNA G1207 methylase RsmC

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RlmG family

Homologues:

Organism=Escherichia coli, GI87082206, Length=377, Percent_Identity=42.1750663129974, Blast_Score=294, Evalue=6e-81,
Organism=Escherichia coli, GI1790830, Length=296, Percent_Identity=28.3783783783784, Blast_Score=95, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMG_ALISL (B6EIC1)

Other databases:

- EMBL:   FM178379
- RefSeq:   YP_002262442.1
- ProteinModelPortal:   B6EIC1
- GeneID:   6986740
- GenomeReviews:   FM178379_GR
- KEGG:   vsa:VSAL_I0947
- HOGENOM:   HBG321931
- OMA:   NRHLGYH
- ProtClustDB:   CLSK874746
- GO:   GO:0005737
- HAMAP:   MF_01859
- InterPro:   IPR017237
- InterPro:   IPR007848
- PIRSF:   PIRSF037565

Pfam domain/function: PF05175 MTS

EC number: =2.1.1.174

Molecular weight: Translated: 43624; Mature: 43624

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTELCLFDRTLNLQRYPKRAQELLQAWDAGDEFIIKHVEEELNLEDNKNILILNDNFGA
CCCCEEEEECCCCHHHCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCEEEEECCCCE
LSCWFSEKHNVTMMTDSFVSQRGTLKNLQRNQCNRVQLISSTEEMPEGFDLVIMQIPKNN
EEEEECCCCCEEEEEHHHHHCCCHHHHHHHCCCCEEEEECCHHCCCCCCEEEEEEECCCC
RMLAWQLQQLRQSMTAECPIIAVNKAKEIHSSTLEVFEDYLGETKTSLAWKKHRLVFSNA
CEEHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHHCCCHHHHHHHEEEEEEECC
NATNPLTIAEAVCWSVDNEDIDLLNYPNVYSGERLDQGARFMLEHIPVDAELRHIIDLGC
CCCCCCHHHHHHHHCCCCCCEEEECCCCCCCCCHHCHHHHHHHHHCCCCHHHHHHHEECC
GNGVLSVKAAQLNPEARITCIDESFMAVESARRNLEVNLGKERQFQFIANNCLDGFKKHS
CCCEEEEEEEECCCCCEEEEECHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHHCCC
SYLVLCNPPFHQGQAVTDHIAWQMFCDAKHILCKEGKLLVIGNRHLDYDDKLCRLFGEEN
CEEEEECCCCCCCCHHHHHHHHHHHHCCHHHEECCCCEEEEECCCCCHHHHHHHHHCCCC
VTTIASNSKFVILEAVKAEKSK
EEEEECCCCEEEEEEECCCCCC
>Mature Secondary Structure
MKTELCLFDRTLNLQRYPKRAQELLQAWDAGDEFIIKHVEEELNLEDNKNILILNDNFGA
CCCCEEEEECCCCHHHCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCEEEEECCCCE
LSCWFSEKHNVTMMTDSFVSQRGTLKNLQRNQCNRVQLISSTEEMPEGFDLVIMQIPKNN
EEEEECCCCCEEEEEHHHHHCCCHHHHHHHCCCCEEEEECCHHCCCCCCEEEEEEECCCC
RMLAWQLQQLRQSMTAECPIIAVNKAKEIHSSTLEVFEDYLGETKTSLAWKKHRLVFSNA
CEEHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHHCCCHHHHHHHEEEEEEECC
NATNPLTIAEAVCWSVDNEDIDLLNYPNVYSGERLDQGARFMLEHIPVDAELRHIIDLGC
CCCCCCHHHHHHHHCCCCCCEEEECCCCCCCCCHHCHHHHHHHHHCCCCHHHHHHHEECC
GNGVLSVKAAQLNPEARITCIDESFMAVESARRNLEVNLGKERQFQFIANNCLDGFKKHS
CCCEEEEEEEECCCCCEEEEECHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHHCCC
SYLVLCNPPFHQGQAVTDHIAWQMFCDAKHILCKEGKLLVIGNRHLDYDDKLCRLFGEEN
CEEEEECCCCCCCCHHHHHHHHHHHHCCHHHEECCCCEEEEECCCCCHHHHHHHHHCCCC
VTTIASNSKFVILEAVKAEKSK
EEEEECCCCEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA