Click here to switch to the map view.

The map label for this gene is rotA [H]

Identifier: 209694512

GI number: 209694512

Start: 1043240

End: 1043797

Strand: Reverse

Name: rotA [H]

Synonym: VSAL_I0945

Alternate gene names: 209694512

Gene position: 1043797-1043240 (Counterclockwise)

Preceding gene: 209694513

Following gene: 209694511

Centisome position: 31.39

GC content: 39.78

Gene sequence:

>558_bases
ATGAAACTAACATCAAAAGTGGCTCTTTTAATTGCCGCACTTGGCTTCAATCAATCAGCATTGGCTGAATCCGTAAAATT
TGATACCACATTAGGGAGCTTTACTATTGAGCTCAATGCAGAGAAAGCGCCAATTTCTGTCGCTAACTTTATTCGCTATG
TCGAAGATGGAAGCTACGTAGGAACGCAATTCCATCGAGTGATCCCTGGCTTTATGGCTCAAGGCGGCGGTTTTGATAAA
GACATGCTGAAAAAATCGAATTATGGCCCAATTAAAAACGAAGGTTATAACGGTTTAGGTAATGATACCGCTACGGTTGC
AATGGCTCGTACCAATAACCCGGATTCAGCAACTCGTCAGTTTTACATTAACTTAAATGACAATGATTTCTTAAACGCAA
ATCAACGTCCACCAGGTTATGCTGTTTTTGGTAAAGTAACCCAAGGTTTTGACATTATTCAAAAAATGGCCTCAAAACCA
ACCAAAGCCCTTGCCAATGGGATGCGTGATATTCCCGTTGAGCCAATTATCATAACTAAAGCAACATTGATTAAATAA

Upstream 100 bases:

>100_bases
AAGCAATTGAAGGCGTACTTAACGATCTTATCAATGCAGTATTAGATAAGATTGCCGTTGATGAAGAACTTCAAACCTAC
ATTAAAGGTAATTTCTAATC

Downstream 100 bases:

>100_bases
TCAAGTTAAGATAATCCAATTACAGAGCAGCTTTCGAGCTGCTCTGTTGTTTATGCTGAATTATTTCCCTCTTTTATTCC
TATTATAAGAATAACAAGGA

Product: peptidyl-prolyl cis-trans isomerase precursor

Products: NA

Alternate protein names: PPIase; Rotamase [H]

Number of amino acids: Translated: 185; Mature: 185

Protein sequence:

>185_residues
MKLTSKVALLIAALGFNQSALAESVKFDTTLGSFTIELNAEKAPISVANFIRYVEDGSYVGTQFHRVIPGFMAQGGGFDK
DMLKKSNYGPIKNEGYNGLGNDTATVAMARTNNPDSATRQFYINLNDNDFLNANQRPPGYAVFGKVTQGFDIIQKMASKP
TKALANGMRDIPVEPIIITKATLIK

Sequences:

>Translated_185_residues
MKLTSKVALLIAALGFNQSALAESVKFDTTLGSFTIELNAEKAPISVANFIRYVEDGSYVGTQFHRVIPGFMAQGGGFDK
DMLKKSNYGPIKNEGYNGLGNDTATVAMARTNNPDSATRQFYINLNDNDFLNANQRPPGYAVFGKVTQGFDIIQKMASKP
TKALANGMRDIPVEPIIITKATLIK
>Mature_185_residues
MKLTSKVALLIAALGFNQSALAESVKFDTTLGSFTIELNAEKAPISVANFIRYVEDGSYVGTQFHRVIPGFMAQGGGFDK
DMLKKSNYGPIKNEGYNGLGNDTATVAMARTNNPDSATRQFYINLNDNDFLNANQRPPGYAVFGKVTQGFDIIQKMASKP
TKALANGMRDIPVEPIIITKATLIK

Specific function: PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. This protein is not essential for growth. Presumably plays a role in signal transduction [H]

COG id: COG0652

COG function: function code O; Peptidyl-prolyl cis-trans isomerase (rotamase) - cyclophilin family

Gene ontology:

Cell location: Periplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase cyclophilin-type domain [H]

Homologues:

Organism=Homo sapiens, GI64276486, Length=129, Percent_Identity=37.2093023255814, Blast_Score=64, Evalue=8e-11,
Organism=Escherichia coli, GI1789763, Length=160, Percent_Identity=49.375, Blast_Score=157, Evalue=4e-40,
Organism=Escherichia coli, GI1786736, Length=163, Percent_Identity=46.6257668711656, Blast_Score=134, Evalue=4e-33,
Organism=Caenorhabditis elegans, GI17552780, Length=160, Percent_Identity=30, Blast_Score=67, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI71980594, Length=139, Percent_Identity=32.3741007194245, Blast_Score=65, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI71980590, Length=139, Percent_Identity=32.3741007194245, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI17986117, Length=165, Percent_Identity=29.0909090909091, Blast_Score=66, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015891
- InterPro:   IPR020892
- InterPro:   IPR002130 [H]

Pfam domain/function: PF00160 Pro_isomerase [H]

EC number: =5.2.1.8 [H]

Molecular weight: Translated: 20088; Mature: 20088

Theoretical pI: Translated: 9.76; Mature: 9.76

Prosite motif: PS00170 CSA_PPIASE_1 ; PS50072 CSA_PPIASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLTSKVALLIAALGFNQSALAESVKFDTTLGSFTIELNAEKAPISVANFIRYVEDGSYV
CCCHHHHHHHHHHHCCCHHHHHHHHCCCCEECEEEEEEECCCCCHHHHHHHHHHHCCCCC
GTQFHRVIPGFMAQGGGFDKDMLKKSNYGPIKNEGYNGLGNDTATVAMARTNNPDSATRQ
HHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCEE
FYINLNDNDFLNANQRPPGYAVFGKVTQGFDIIQKMASKPTKALANGMRDIPVEPIIITK
EEEEECCCCCCCCCCCCCCEEEECCHHCCHHHHHHHHCCCHHHHHCCCCCCCCCCEEEEE
ATLIK
EECCC
>Mature Secondary Structure
MKLTSKVALLIAALGFNQSALAESVKFDTTLGSFTIELNAEKAPISVANFIRYVEDGSYV
CCCHHHHHHHHHHHCCCHHHHHHHHCCCCEECEEEEEEECCCCCHHHHHHHHHHHCCCCC
GTQFHRVIPGFMAQGGGFDKDMLKKSNYGPIKNEGYNGLGNDTATVAMARTNNPDSATRQ
HHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCEE
FYINLNDNDFLNANQRPPGYAVFGKVTQGFDIIQKMASKPTKALANGMRDIPVEPIIITK
EEEEECCCCCCCCCCCCCCEEEECCHHCCHHHHHHHHCCCHHHHHCCCCCCCCCCEEEEE
ATLIK
EECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7948017 [H]