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The map label for this gene is ispA [H]

Identifier: 209694502

GI number: 209694502

Start: 1032279

End: 1033175

Strand: Reverse

Name: ispA [H]

Synonym: VSAL_I0934

Alternate gene names: 209694502

Gene position: 1033175-1032279 (Counterclockwise)

Preceding gene: 209694503

Following gene: 209694501

Centisome position: 31.07

GC content: 40.36

Gene sequence:

>897_bases
ATGACTGATTCACTAAAACAACAACTGCCTATTTTTCAACAGCGTAACATTGAACATTTAAATCAATGGTTTGAAAAGAT
TGAAATTCAACAACAACCGCTTACTGATGCAATGAAATACGCATTACTATTAGGTGGCAAACGCGCACGTCCTTATCTCG
TCTATATTACGGCTAACATGTTAGGCATAGATAGTGAGCATGCAGATGCACCTGCGGCGGCAGTTGAATGTATTCATTCT
TACTCATTAATTCATGATGATTTACCAGCTATGGATAATGACGATTTACGTCGTGGTCATGCGACTTGTCATATTAAATT
CGATGAAGCAACAGCCATTCTCGCTGGCGATGCATTGCAAACCTTAGCCTTTGAAATTTTGGCTAACGGTACCCTAGCAG
AAGAAGCAGAATCCTCGCGTATTGATATGATCCGAGTATTATCTAAAGCATCAGGGGCATCAGGGATGTGTATGGGTCAA
TCACTTGACCTACAAGCCGAAGGGAAGCAAGTTGATTTACTCTCTTTAGAGCAAATCCATGCCAACAAAACCGGTGCATT
AATGAAAAGTGCCATTCAACTTGGTGCGTTATGTGCTGGTGAAAAAGGCAAACAAATACTTCCACAACTTGAAAAATACG
CAGACGCTATTGGTCTCGCCTTTCAAGTTCAAGATGATATCTTAGATATCACAAGTGACACCGAAACATTAGGAAAACCT
CAAGGCTCTGATATAGCCTTAGAGAAAAGTACCTATCCTGCTTTATTAGGCCTTGATGGTGCTAAACTGAAAGCAGAACA
ATTATACCAAACGGCTATCACTGCATTAGATGATATTCCATATAACACGGATGCAATGCAAGCGTTCGCTCAATACGTCA
TCGAGCGTAAAAACTAA

Upstream 100 bases:

>100_bases
GCACGAGCAGGTCAAGAAAAACTGACTCAAGCTGAACAACGTGTCGATATTCTCCTTCAAGCTGATGATAATGCAGAACT
AACCCCTTTTGATGCCAATG

Downstream 100 bases:

>100_bases
AACAGATAAGCGCTGAATAATTATGTCACTTGATATTTCAAAATACCCAATTCTGGCTTTGGCAAACACACCAGATGAAC
TACGTTCTTTGCCAAAAGAA

Product: geranyltranstransferase

Products: NA

Alternate protein names: FPP synthase; (2E,6E)-farnesyl diphosphate synthase; Geranyltranstransferase [H]

Number of amino acids: Translated: 298; Mature: 297

Protein sequence:

>298_residues
MTDSLKQQLPIFQQRNIEHLNQWFEKIEIQQQPLTDAMKYALLLGGKRARPYLVYITANMLGIDSEHADAPAAAVECIHS
YSLIHDDLPAMDNDDLRRGHATCHIKFDEATAILAGDALQTLAFEILANGTLAEEAESSRIDMIRVLSKASGASGMCMGQ
SLDLQAEGKQVDLLSLEQIHANKTGALMKSAIQLGALCAGEKGKQILPQLEKYADAIGLAFQVQDDILDITSDTETLGKP
QGSDIALEKSTYPALLGLDGAKLKAEQLYQTAITALDDIPYNTDAMQAFAQYVIERKN

Sequences:

>Translated_298_residues
MTDSLKQQLPIFQQRNIEHLNQWFEKIEIQQQPLTDAMKYALLLGGKRARPYLVYITANMLGIDSEHADAPAAAVECIHS
YSLIHDDLPAMDNDDLRRGHATCHIKFDEATAILAGDALQTLAFEILANGTLAEEAESSRIDMIRVLSKASGASGMCMGQ
SLDLQAEGKQVDLLSLEQIHANKTGALMKSAIQLGALCAGEKGKQILPQLEKYADAIGLAFQVQDDILDITSDTETLGKP
QGSDIALEKSTYPALLGLDGAKLKAEQLYQTAITALDDIPYNTDAMQAFAQYVIERKN
>Mature_297_residues
TDSLKQQLPIFQQRNIEHLNQWFEKIEIQQQPLTDAMKYALLLGGKRARPYLVYITANMLGIDSEHADAPAAAVECIHSY
SLIHDDLPAMDNDDLRRGHATCHIKFDEATAILAGDALQTLAFEILANGTLAEEAESSRIDMIRVLSKASGASGMCMGQS
LDLQAEGKQVDLLSLEQIHANKTGALMKSAIQLGALCAGEKGKQILPQLEKYADAIGLAFQVQDDILDITSDTETLGKPQ
GSDIALEKSTYPALLGLDGAKLKAEQLYQTAITALDDIPYNTDAMQAFAQYVIERKN

Specific function: Unknown

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786623, Length=297, Percent_Identity=55.8922558922559, Blast_Score=305, Evalue=2e-84,
Organism=Escherichia coli, GI1789578, Length=249, Percent_Identity=30.1204819277108, Blast_Score=103, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17505681, Length=185, Percent_Identity=27.5675675675676, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24651612, Length=186, Percent_Identity=29.0322580645161, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.10 [H]

Molecular weight: Translated: 32534; Mature: 32403

Theoretical pI: Translated: 4.50; Mature: 4.50

Prosite motif: PS00723 POLYPRENYL_SYNTHET_1 ; PS00444 POLYPRENYL_SYNTHET_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDSLKQQLPIFQQRNIEHLNQWFEKIEIQQQPLTDAMKYALLLGGKRARPYLVYITANM
CCCCHHHHCCHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCEEEEEEECE
LGIDSEHADAPAAAVECIHSYSLIHDDLPAMDNDDLRRGHATCHIKFDEATAILAGDALQ
EECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCEEEEEEECCCEEEEEHHHHH
TLAFEILANGTLAEEAESSRIDMIRVLSKASGASGMCMGQSLDLQAEGKQVDLLSLEQIH
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCEEEEEEHHHHH
ANKTGALMKSAIQLGALCAGEKGKQILPQLEKYADAIGLAFQVQDDILDITSDTETLGKP
CCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHEEEEECCHHEECCCCHHHHCCC
QGSDIALEKSTYPALLGLDGAKLKAEQLYQTAITALDDIPYNTDAMQAFAQYVIERKN
CCCCEEEECCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TDSLKQQLPIFQQRNIEHLNQWFEKIEIQQQPLTDAMKYALLLGGKRARPYLVYITANM
CCCHHHHCCHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCEEEEEEECE
LGIDSEHADAPAAAVECIHSYSLIHDDLPAMDNDDLRRGHATCHIKFDEATAILAGDALQ
EECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCEEEEEEECCCEEEEEHHHHH
TLAFEILANGTLAEEAESSRIDMIRVLSKASGASGMCMGQSLDLQAEGKQVDLLSLEQIH
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCEEEEEEHHHHH
ANKTGALMKSAIQLGALCAGEKGKQILPQLEKYADAIGLAFQVQDDILDITSDTETLGKP
CCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHEEEEECCHHEECCCCHHHHCCC
QGSDIALEKSTYPALLGLDGAKLKAEQLYQTAITALDDIPYNTDAMQAFAQYVIERKN
CCCCEEEECCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2089044; 9278503 [H]