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The map label for this gene is purU [H]

Identifier: 209694483

GI number: 209694483

Start: 1012702

End: 1013535

Strand: Direct

Name: purU [H]

Synonym: VSAL_I0912

Alternate gene names: 209694483

Gene position: 1012702-1013535 (Clockwise)

Preceding gene: 209694477

Following gene: 209694486

Centisome position: 30.46

GC content: 37.53

Gene sequence:

>834_bases
ATGGAAAAGAAAATATTATTAACGGATTGCCCTGATTCTCGTGGGCTAATCGCCAAAATAACCAACATTTGTTACAAACA
TCAACTGAACATTGTTCACAATAATGAATACGTAGACAACGTTACGGGTCAATTTTTCATGAGAACGGAACTTGAAGGGA
TCTTTAATGATGAAACCTTCTTACTCGATATTGATCAAGCATTGCCGCCAGGCAGCCAACGAAAGTTAGTATTAGAACCA
AAAAAGAAAGTCGTTATTCTTGTCACTAAAGAAGCTCATTGTATTGGCGATATTCTAATCAAAGCCTATTCTGGTGCGAT
GAACATCGAGATCTCGGCGGTTATTGGCAACCACGATACATTAGGTGCCTTGATTGAGAAGTTTGATATTCCTTTTCATT
ATGTATCTCATGAAGGCTTGTCACGTGGCGAGCATGAAGACAAAATGCTAAGCATTATTCATTCATACGATCCTGAATAC
GTCGTTTTAGCAAAATACATGCGTGTACTTACCCCTGAATTTGTTGAGCAATTTCCAAAAAGAATCATCAATATCCATCA
CAGTTTCTTACCTGCATTCATTGGTGCAAAACCGTATCAGCAAGCTTATGATCGTGGTGTGAAAATCATTGGTGCCACAG
CACACTTTGTCACCAATAATTTAGATGAAGGTCCAATCATCAAACAAGATGTTATCCCTATTGATCATAACTTCAGTGCA
GAAGATATGGCAATGGCAGGACGTGACGTTGAGAAATCAGTATTAAGTAAAGCCTTAACAAAAGTTCTGAATGACCATGT
ATTTGTTTATGGTAATAAGACCGTTATTCTTTAA

Upstream 100 bases:

>100_bases
GATTTTTCAGGGCGAAAAACACTTTACGTTCTCTTCTTTTGTCGGTACAACCATAGACAGTCCAAATTTATAGACACACT
TTGTGAGCACGAGAGATATA

Downstream 100 bases:

>100_bases
ATAATGTCGTAAATACAAAAAAACGGAGATCATCATGATCTCCGTTTTTATTTGTTCTTAATTTGAATTCAATTATTGTG
CAGTAATAAACTCTATCGCG

Product: formyltetrahydrofolate deformylase

Products: NA

Alternate protein names: Formyl-FH(4) hydrolase [H]

Number of amino acids: Translated: 277; Mature: 277

Protein sequence:

>277_residues
MEKKILLTDCPDSRGLIAKITNICYKHQLNIVHNNEYVDNVTGQFFMRTELEGIFNDETFLLDIDQALPPGSQRKLVLEP
KKKVVILVTKEAHCIGDILIKAYSGAMNIEISAVIGNHDTLGALIEKFDIPFHYVSHEGLSRGEHEDKMLSIIHSYDPEY
VVLAKYMRVLTPEFVEQFPKRIINIHHSFLPAFIGAKPYQQAYDRGVKIIGATAHFVTNNLDEGPIIKQDVIPIDHNFSA
EDMAMAGRDVEKSVLSKALTKVLNDHVFVYGNKTVIL

Sequences:

>Translated_277_residues
MEKKILLTDCPDSRGLIAKITNICYKHQLNIVHNNEYVDNVTGQFFMRTELEGIFNDETFLLDIDQALPPGSQRKLVLEP
KKKVVILVTKEAHCIGDILIKAYSGAMNIEISAVIGNHDTLGALIEKFDIPFHYVSHEGLSRGEHEDKMLSIIHSYDPEY
VVLAKYMRVLTPEFVEQFPKRIINIHHSFLPAFIGAKPYQQAYDRGVKIIGATAHFVTNNLDEGPIIKQDVIPIDHNFSA
EDMAMAGRDVEKSVLSKALTKVLNDHVFVYGNKTVIL
>Mature_277_residues
MEKKILLTDCPDSRGLIAKITNICYKHQLNIVHNNEYVDNVTGQFFMRTELEGIFNDETFLLDIDQALPPGSQRKLVLEP
KKKVVILVTKEAHCIGDILIKAYSGAMNIEISAVIGNHDTLGALIEKFDIPFHYVSHEGLSRGEHEDKMLSIIHSYDPEY
VVLAKYMRVLTPEFVEQFPKRIINIHHSFLPAFIGAKPYQQAYDRGVKIIGATAHFVTNNLDEGPIIKQDVIPIDHNFSA
EDMAMAGRDVEKSVLSKALTKVLNDHVFVYGNKTVIL

Specific function: Produces formate from formyl-tetrahydrofolate. Provides the major source of formate for the purT-dependent synthesis of 5'-phosphoribosyl-N-formylglycinamide (FGAR) during aerobic growth. Has a role in regulating the one-carbon pool [H]

COG id: COG0788

COG function: function code F; Formyltetrahydrofolate hydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=190, Percent_Identity=26.3157894736842, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI209869995, Length=190, Percent_Identity=26.3157894736842, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI209869993, Length=190, Percent_Identity=26.3157894736842, Blast_Score=86, Evalue=3e-17,
Organism=Escherichia coli, GI1787483, Length=277, Percent_Identity=69.6750902527076, Blast_Score=417, Evalue=1e-118,
Organism=Escherichia coli, GI1788846, Length=103, Percent_Identity=38.8349514563107, Blast_Score=82, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17567511, Length=160, Percent_Identity=36.25, Blast_Score=102, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24582400, Length=196, Percent_Identity=26.0204081632653, Blast_Score=76, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR002376
- InterPro:   IPR004810 [H]

Pfam domain/function: PF01842 ACT; PF00551 Formyl_trans_N [H]

EC number: =3.5.1.10 [H]

Molecular weight: Translated: 31257; Mature: 31257

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKKILLTDCPDSRGLIAKITNICYKHQLNIVHNNEYVDNVTGQFFMRTELEGIFNDETF
CCCCEEEEECCCCCCHHHHHHHHHHHHEEEEEECCCCCCCCCCCEEEEEECCCEECCCEE
LLDIDQALPPGSQRKLVLEPKKKVVILVTKEAHCIGDILIKAYSGAMNIEISAVIGNHDT
EEEEHHCCCCCCCCEEEECCCCCEEEEEECCCHHHHHHHHHHHCCCEEEEEEEEECCCHH
LGALIEKFDIPFHYVSHEGLSRGEHEDKMLSIIHSYDPEYVVLAKYMRVLTPEFVEQFPK
HHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHCHHHHHHHHH
RIINIHHSFLPAFIGAKPYQQAYDRGVKIIGATAHFVTNNLDEGPIIKQDVIPIDHNFSA
HHHHHHHHHHHHHHCCCHHHHHHHCCEEEEEEEHHHHHCCCCCCCCCCCCCCCCCCCCCH
EDMAMAGRDVEKSVLSKALTKVLNDHVFVYGNKTVIL
HHHHHCCCHHHHHHHHHHHHHHHCCCEEEEECCEEEC
>Mature Secondary Structure
MEKKILLTDCPDSRGLIAKITNICYKHQLNIVHNNEYVDNVTGQFFMRTELEGIFNDETF
CCCCEEEEECCCCCCHHHHHHHHHHHHEEEEEECCCCCCCCCCCEEEEEECCCEECCCEE
LLDIDQALPPGSQRKLVLEPKKKVVILVTKEAHCIGDILIKAYSGAMNIEISAVIGNHDT
EEEEHHCCCCCCCCEEEECCCCCEEEEEECCCHHHHHHHHHHHCCCEEEEEEEEECCCHH
LGALIEKFDIPFHYVSHEGLSRGEHEDKMLSIIHSYDPEYVVLAKYMRVLTPEFVEQFPK
HHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHCHHHHHHHHH
RIINIHHSFLPAFIGAKPYQQAYDRGVKIIGATAHFVTNNLDEGPIIKQDVIPIDHNFSA
HHHHHHHHHHHHHHCCCHHHHHHHCCEEEEEEEHHHHHCCCCCCCCCCCCCCCCCCCCCH
EDMAMAGRDVEKSVLSKALTKVLNDHVFVYGNKTVIL
HHHHHCCCHHHHHHHHHHHHHHHCCCEEEEECCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]