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The map label for this gene is proS

Identifier: 209694468

GI number: 209694468

Start: 992979

End: 994694

Strand: Direct

Name: proS

Synonym: VSAL_I0892

Alternate gene names: 209694468

Gene position: 992979-994694 (Clockwise)

Preceding gene: 209694467

Following gene: 209694470

Centisome position: 29.86

GC content: 45.28

Gene sequence:

>1716_bases
ATGCGCACTAGCAAATACCTTCTTTCAACACTGAAGGAGACTCCAAACGACGCAGAAGTAGTGAGCCACCAGCTCATGCT
ACGTGCAGGTATGATCCGTCGTCTAGCTTCAGGTTTATATACTTGGCTACCGACTGGTCTACGTGTATTGCGTAAAGTCG
AAAATATCGTTCGTCAAGAGATCGACAATGCAGGTGCAATCGAAACGTTGATGCCCGTAGTTCAGCCGTTTGAGCTTTGG
GAAGAAACAGGCCGTTCTGAAGAGATGGGGCCTGAACTGCTTCGTTTTACTGACCGTCATGCTCGTCCATTTGTACTTAG
CCCAACGGCTGAAGAAGTTATCACTAGCCTTGTGCGTAACGAAGTGAACTCTTACAAGCAACTTCCGCTAAACCTATACC
AAATCCAAACTAAATTCCGTGATGAACGTCGTCCACGCTTTGGTGTAATGCGTGCACGTGAATTCTGCATGATGGATGCG
TACAGCTTCGATATCGATAAAGAAGGCCTACAAAAGTCTTACGATGCGATGCACGATGCTTACTGCAGAGCATTCGACCG
TATGGGTCTTGAGTACCGTCCAGTATTGGCTGACTCAGGCGCTATCGGCGGCAGTGGTTCTCAAGAGTTCCACGTACTTG
CAGAAAGCGGCGAAGATTTAATCGCATTCTCAACAGAATCTGATTACGCTGCAAACATCGAAAAAGCAGAAGCACTAGCA
CCGACAACTGAACGTGCTGCTCCTACTCAAGAGATGACAACGGTTGATACACCAAATGCAAAAACAATTGCAGAATTGGT
TGAGCAACACGGTATCGCAATTGAAAAAACAGTGAAAACATTATTTGTTAAAGCATCTGATGAAGTTGATGCTGATATCA
TTGCACTTATCATCCGTGGTGATCACGAGCTTAACGAAGTTAAAGCTGAAAACCTTCCACACGTTTTATCTCCACTAGAG
ATGGCTGACGAAGCACAACTTCGTGATCTAATCGGTGCTGGTGCTGGTTCTCTTGGTCCTGTAGGCCTAGAACTGCCATT
CATCGTTGACCGCTCTGTTGCGGTAATGAGTGATTTTAGTACAGGCGCAAACATCGATGGTAAACACTTCTTTGGTGTGA
ACTGGGATCGTGATGTACAGCTTGGTCAAATTGAAGATTTACGCAGCGTAGTTGAAGGTGACCTAAGCCCATGTGGCCAA
GGTACTCTACAACTGAAACGCGGTATCGAAGTCGGTCACATCTTCCAATTAGGTACAGCCTACTCAGAAGCAATGAACTG
TGGCGTACTTGATGCTAACGGTAAAAACTCTATCCTTGAGATGGGTTGTTACGGTATCGGTGTTTCACGTGTTGTTGCAT
CAGCTATCGAGCAAAACAATGACGAATACGGCATTGTATGGCCAGAAGCGCTTGCACCATTTACGGTTGCTATCGTTCCA
ATGAACATGTACAAATCTGATCGCGTTAAAGAAGCGGCAGAAAAACTGTATGCAGAATTAACAGCGATGGGTATCGACGT
TCTATTTGATGATCGTAAAGAGCGCCCAGGTGTTATGTTCAAGGATATCGAGCTTATCGGTATTCCACATACCGTTGTGA
TCGGCGATCGTAGCATGGACGAAGGTAACTTTGAGTATAAGAACCGTCGTGCAAACAGCAAAGAAGTAATCGAAATTGCA
AACATCGTTGAGCACATTAAAGCACAACTAAGCTAA

Upstream 100 bases:

>100_bases
GCCCATTCTTAAAGAACATACTCTTACTAAAATTGAACTCTCGGTTTAACTGTTTAAATCGAGCTATTCCAATATTCATA
ACTAACAAATGGATGAAAAC

Downstream 100 bases:

>100_bases
TTCGACACTTCATCAAACACAAAAAGCTAAGTCGTATGACTTAGCTTTTTTTATGCATTAAATCAGATAAATTAACTGAG
TAAGCTGCGATGTACCTTAA

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS

Number of amino acids: Translated: 571; Mature: 571

Protein sequence:

>571_residues
MRTSKYLLSTLKETPNDAEVVSHQLMLRAGMIRRLASGLYTWLPTGLRVLRKVENIVRQEIDNAGAIETLMPVVQPFELW
EETGRSEEMGPELLRFTDRHARPFVLSPTAEEVITSLVRNEVNSYKQLPLNLYQIQTKFRDERRPRFGVMRAREFCMMDA
YSFDIDKEGLQKSYDAMHDAYCRAFDRMGLEYRPVLADSGAIGGSGSQEFHVLAESGEDLIAFSTESDYAANIEKAEALA
PTTERAAPTQEMTTVDTPNAKTIAELVEQHGIAIEKTVKTLFVKASDEVDADIIALIIRGDHELNEVKAENLPHVLSPLE
MADEAQLRDLIGAGAGSLGPVGLELPFIVDRSVAVMSDFSTGANIDGKHFFGVNWDRDVQLGQIEDLRSVVEGDLSPCGQ
GTLQLKRGIEVGHIFQLGTAYSEAMNCGVLDANGKNSILEMGCYGIGVSRVVASAIEQNNDEYGIVWPEALAPFTVAIVP
MNMYKSDRVKEAAEKLYAELTAMGIDVLFDDRKERPGVMFKDIELIGIPHTVVIGDRSMDEGNFEYKNRRANSKEVIEIA
NIVEHIKAQLS

Sequences:

>Translated_571_residues
MRTSKYLLSTLKETPNDAEVVSHQLMLRAGMIRRLASGLYTWLPTGLRVLRKVENIVRQEIDNAGAIETLMPVVQPFELW
EETGRSEEMGPELLRFTDRHARPFVLSPTAEEVITSLVRNEVNSYKQLPLNLYQIQTKFRDERRPRFGVMRAREFCMMDA
YSFDIDKEGLQKSYDAMHDAYCRAFDRMGLEYRPVLADSGAIGGSGSQEFHVLAESGEDLIAFSTESDYAANIEKAEALA
PTTERAAPTQEMTTVDTPNAKTIAELVEQHGIAIEKTVKTLFVKASDEVDADIIALIIRGDHELNEVKAENLPHVLSPLE
MADEAQLRDLIGAGAGSLGPVGLELPFIVDRSVAVMSDFSTGANIDGKHFFGVNWDRDVQLGQIEDLRSVVEGDLSPCGQ
GTLQLKRGIEVGHIFQLGTAYSEAMNCGVLDANGKNSILEMGCYGIGVSRVVASAIEQNNDEYGIVWPEALAPFTVAIVP
MNMYKSDRVKEAAEKLYAELTAMGIDVLFDDRKERPGVMFKDIELIGIPHTVVIGDRSMDEGNFEYKNRRANSKEVIEIA
NIVEHIKAQLS
>Mature_571_residues
MRTSKYLLSTLKETPNDAEVVSHQLMLRAGMIRRLASGLYTWLPTGLRVLRKVENIVRQEIDNAGAIETLMPVVQPFELW
EETGRSEEMGPELLRFTDRHARPFVLSPTAEEVITSLVRNEVNSYKQLPLNLYQIQTKFRDERRPRFGVMRAREFCMMDA
YSFDIDKEGLQKSYDAMHDAYCRAFDRMGLEYRPVLADSGAIGGSGSQEFHVLAESGEDLIAFSTESDYAANIEKAEALA
PTTERAAPTQEMTTVDTPNAKTIAELVEQHGIAIEKTVKTLFVKASDEVDADIIALIIRGDHELNEVKAENLPHVLSPLE
MADEAQLRDLIGAGAGSLGPVGLELPFIVDRSVAVMSDFSTGANIDGKHFFGVNWDRDVQLGQIEDLRSVVEGDLSPCGQ
GTLQLKRGIEVGHIFQLGTAYSEAMNCGVLDANGKNSILEMGCYGIGVSRVVASAIEQNNDEYGIVWPEALAPFTVAIVP
MNMYKSDRVKEAAEKLYAELTAMGIDVLFDDRKERPGVMFKDIELIGIPHTVVIGDRSMDEGNFEYKNRRANSKEVIEIA
NIVEHIKAQLS

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids su

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily

Homologues:

Organism=Homo sapiens, GI34303926, Length=226, Percent_Identity=42.9203539823009, Blast_Score=201, Evalue=2e-51,
Organism=Escherichia coli, GI1786392, Length=570, Percent_Identity=68.7719298245614, Blast_Score=824, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115532348, Length=264, Percent_Identity=37.5, Blast_Score=169, Evalue=3e-42,
Organism=Caenorhabditis elegans, GI193203271, Length=105, Percent_Identity=37.1428571428571, Blast_Score=79, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6320931, Length=547, Percent_Identity=30.7129798903108, Blast_Score=239, Evalue=7e-64,
Organism=Drosophila melanogaster, GI24656200, Length=225, Percent_Identity=36.4444444444444, Blast_Score=156, Evalue=3e-38,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): SYP_ALISL (B6EHZ8)

Other databases:

- EMBL:   FM178379
- RefSeq:   YP_002262396.1
- GeneID:   6986711
- GenomeReviews:   FM178379_GR
- KEGG:   vsa:VSAL_I0892
- HOGENOM:   HBG403504
- OMA:   DFVLGPT
- ProtClustDB:   PRK09194
- GO:   GO:0005737
- HAMAP:   MF_01569
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- InterPro:   IPR007214
- Gene3D:   G3DSA:3.40.50.800
- Gene3D:   G3DSA:3.90.960.10
- PRINTS:   PR01046
- TIGRFAMs:   TIGR00409

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF04073 YbaK; SSF52954 Anticodon_bd; SSF55826 YbaK/aa-tRNA-synth-assoc-reg

EC number: =6.1.1.15

Molecular weight: Translated: 63369; Mature: 63369

Theoretical pI: Translated: 4.57; Mature: 4.57

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTSKYLLSTLKETPNDAEVVSHQLMLRAGMIRRLASGLYTWLPTGLRVLRKVENIVRQE
CCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IDNAGAIETLMPVVQPFELWEETGRSEEMGPELLRFTDRHARPFVLSPTAEEVITSLVRN
HCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCEEECCCHHHHHHHHHHH
EVNSYKQLPLNLYQIQTKFRDERRPRFGVMRAREFCMMDAYSFDIDKEGLQKSYDAMHDA
HHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
YCRAFDRMGLEYRPVLADSGAIGGSGSQEFHVLAESGEDLIAFSTESDYAANIEKAEALA
HHHHHHHCCCEEECEEECCCCCCCCCCCCEEEEECCCCCEEEEECCCCCCCCCHHHHHCC
PTTERAAPTQEMTTVDTPNAKTIAELVEQHGIAIEKTVKTLFVKASDEVDADIIALIIRG
CCCHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHEEECCCCCCCEEEEEEEEC
DHELNEVKAENLPHVLSPLEMADEAQLRDLIGAGAGSLGPVGLELPFIVDRSVAVMSDFS
CCHHHHHHHCCCCHHHCHHHHHHHHHHHHHHHCCCCCCCCCCEECCEEECCCHHHHCCCC
TGANIDGKHFFGVNWDRDVQLGQIEDLRSVVEGDLSPCGQGTLQLKRGIEVGHIFQLGTA
CCCCCCCCEEECCCCCCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHCCCCHHHHHHHHHH
YSEAMNCGVLDANGKNSILEMGCYGIGVSRVVASAIEQNNDEYGIVWPEALAPFTVAIVP
HHHHCCCCEECCCCCCCHHHHCCHHCCHHHHHHHHHHCCCCCEEEECHHHHCCEEEEEEE
MNMYKSDRVKEAAEKLYAELTAMGIDVLFDDRKERPGVMFKDIELIGIPHTVVIGDRSMD
HHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCEEECEEEEECCEEEEECCCCCC
EGNFEYKNRRANSKEVIEIANIVEHIKAQLS
CCCCCHHHCCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRTSKYLLSTLKETPNDAEVVSHQLMLRAGMIRRLASGLYTWLPTGLRVLRKVENIVRQE
CCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IDNAGAIETLMPVVQPFELWEETGRSEEMGPELLRFTDRHARPFVLSPTAEEVITSLVRN
HCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCEEECCCHHHHHHHHHHH
EVNSYKQLPLNLYQIQTKFRDERRPRFGVMRAREFCMMDAYSFDIDKEGLQKSYDAMHDA
HHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
YCRAFDRMGLEYRPVLADSGAIGGSGSQEFHVLAESGEDLIAFSTESDYAANIEKAEALA
HHHHHHHCCCEEECEEECCCCCCCCCCCCEEEEECCCCCEEEEECCCCCCCCCHHHHHCC
PTTERAAPTQEMTTVDTPNAKTIAELVEQHGIAIEKTVKTLFVKASDEVDADIIALIIRG
CCCHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHEEECCCCCCCEEEEEEEEC
DHELNEVKAENLPHVLSPLEMADEAQLRDLIGAGAGSLGPVGLELPFIVDRSVAVMSDFS
CCHHHHHHHCCCCHHHCHHHHHHHHHHHHHHHCCCCCCCCCCEECCEEECCCHHHHCCCC
TGANIDGKHFFGVNWDRDVQLGQIEDLRSVVEGDLSPCGQGTLQLKRGIEVGHIFQLGTA
CCCCCCCCEEECCCCCCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHCCCCHHHHHHHHHH
YSEAMNCGVLDANGKNSILEMGCYGIGVSRVVASAIEQNNDEYGIVWPEALAPFTVAIVP
HHHHCCCCEECCCCCCCHHHHCCHHCCHHHHHHHHHHCCCCCEEEECHHHHCCEEEEEEE
MNMYKSDRVKEAAEKLYAELTAMGIDVLFDDRKERPGVMFKDIELIGIPHTVVIGDRSMD
HHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCEEECEEEEECCEEEEECCCCCC
EGNFEYKNRRANSKEVIEIANIVEHIKAQLS
CCCCCHHHCCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA