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The map label for this gene is metR [H]

Identifier: 209694439

GI number: 209694439

Start: 958840

End: 959751

Strand: Reverse

Name: metR [H]

Synonym: VSAL_I0860

Alternate gene names: 209694439

Gene position: 959751-958840 (Counterclockwise)

Preceding gene: 209694441

Following gene: 209694436

Centisome position: 28.86

GC content: 39.36

Gene sequence:

>912_bases
ATGATTGAATTAAAACACCTAAAAACCTTAGTCACACTTAGAGATACGGGATCATTAACCGCAACAGCAAATGCCCTACA
CCTCACTCAATCAGCACTGTCTCATCAATTAAAAGATTTTGAAACTCGCCTTGGTGGCACTGTTTTTTTACGAAAAACTC
GCCCAGTCAAATTTACGTCTGAAGGTGAAATACTGTTAACTCTTGCTGATGATATTTTGCCTAAAATGGCCAGAGCTGAA
TATGACATCGCAAGTTTAAAAGAAGATCTTCATGGAAGGCTGCACATGGCTATCGAATGTCATTCTTGCTTTCAATGGTT
AATGCCTGCAATTAAAGAGTATCAAGTCGGTTGGCCAGAAGTAGAATTGGATTTTTCATCTGGCTTTGGTTTTGAACCTT
TACCGGCATTAGTTAATGGAGATTTAGATCTGGTTATCACTTCTGATATTCAACCTCGTAATGAAGTACATTACGAACCT
TTGTTCGATTTTGAAATGCGCCTGGTCATGGCAACCAATCACCCTCTTGCAAGTAAAGAATACATTGAACCGGAAGATTT
GATCGATCAAACCATGATGACGTACCCAGTACAAAAGCAACGATTGGACGTGGTTAAGCATTTTTTATCGCCAAACAACG
TTGAACCCGCTAAATGGAAGCAGGCAGAAAACACATTAATGTTAATACAGATGGTATCCGCAGGATTAGGGGTTGCAGCA
TTGCCAAATTGGGCAATTAGCGAATTTTCACGACAGGGGTTGATTGAAAGTAAACCATTAGGAAAAGGGTTATGGCGAAG
ATTGTTCGCTGCCGTTAGAGACAGCGAACATGATAAAAAATATTTACAAGCATTTTTCTCTACTGCGAAACAGCAGAGCC
AGAGTCATTTAGATGGTATTAAAATGACTTAA

Upstream 100 bases:

>100_bases
AGTATTAACATCATTTAGCCGTCTAGATGTTTACTTTGAAGGTTTTTCACTTTACTGTGAAGATTATTGAACTGACTCAA
ACGGAACTTGAGGAATTATA

Downstream 100 bases:

>100_bases
ATGAGGTATGTTTAAAATCATTTATTCTTGAATTGATTCGTTAATGGGTCATATTTATAACCCAAGCCACTGAGCTTGTC
TTGAACTCCTTCTGTATCAA

Product: transcriptional activator MetR

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 303; Mature: 303

Protein sequence:

>303_residues
MIELKHLKTLVTLRDTGSLTATANALHLTQSALSHQLKDFETRLGGTVFLRKTRPVKFTSEGEILLTLADDILPKMARAE
YDIASLKEDLHGRLHMAIECHSCFQWLMPAIKEYQVGWPEVELDFSSGFGFEPLPALVNGDLDLVITSDIQPRNEVHYEP
LFDFEMRLVMATNHPLASKEYIEPEDLIDQTMMTYPVQKQRLDVVKHFLSPNNVEPAKWKQAENTLMLIQMVSAGLGVAA
LPNWAISEFSRQGLIESKPLGKGLWRRLFAAVRDSEHDKKYLQAFFSTAKQQSQSHLDGIKMT

Sequences:

>Translated_303_residues
MIELKHLKTLVTLRDTGSLTATANALHLTQSALSHQLKDFETRLGGTVFLRKTRPVKFTSEGEILLTLADDILPKMARAE
YDIASLKEDLHGRLHMAIECHSCFQWLMPAIKEYQVGWPEVELDFSSGFGFEPLPALVNGDLDLVITSDIQPRNEVHYEP
LFDFEMRLVMATNHPLASKEYIEPEDLIDQTMMTYPVQKQRLDVVKHFLSPNNVEPAKWKQAENTLMLIQMVSAGLGVAA
LPNWAISEFSRQGLIESKPLGKGLWRRLFAAVRDSEHDKKYLQAFFSTAKQQSQSHLDGIKMT
>Mature_303_residues
MIELKHLKTLVTLRDTGSLTATANALHLTQSALSHQLKDFETRLGGTVFLRKTRPVKFTSEGEILLTLADDILPKMARAE
YDIASLKEDLHGRLHMAIECHSCFQWLMPAIKEYQVGWPEVELDFSSGFGFEPLPALVNGDLDLVITSDIQPRNEVHYEP
LFDFEMRLVMATNHPLASKEYIEPEDLIDQTMMTYPVQKQRLDVVKHFLSPNNVEPAKWKQAENTLMLIQMVSAGLGVAA
LPNWAISEFSRQGLIESKPLGKGLWRRLFAAVRDSEHDKKYLQAFFSTAKQQSQSHLDGIKMT

Specific function: Control of the last step in methionine biosynthesis; metR is a positive activator of the metA, metE and metH genes. It is also a negative regulator of its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790262, Length=301, Percent_Identity=53.8205980066445, Blast_Score=346, Evalue=9e-97,
Organism=Escherichia coli, GI1786401, Length=267, Percent_Identity=26.2172284644195, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI145693105, Length=276, Percent_Identity=23.9130434782609, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1788887, Length=245, Percent_Identity=25.7142857142857, Blast_Score=78, Evalue=8e-16,
Organism=Escherichia coli, GI157672245, Length=231, Percent_Identity=23.8095238095238, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1790399, Length=256, Percent_Identity=25.390625, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1788297, Length=247, Percent_Identity=26.3157894736842, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1790208, Length=251, Percent_Identity=24.7011952191235, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1788748, Length=243, Percent_Identity=22.2222222222222, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI145693193, Length=267, Percent_Identity=25.4681647940075, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1787601, Length=177, Percent_Identity=24.8587570621469, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1787879, Length=260, Percent_Identity=21.9230769230769, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1788296, Length=185, Percent_Identity=27.5675675675676, Blast_Score=61, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34363; Mature: 34363

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: PS50931 HTH_LYSR ; PS00300 SRP54

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIELKHLKTLVTLRDTGSLTATANALHLTQSALSHQLKDFETRLGGTVFLRKTRPVKFTS
CCCHHHHHHHEEEECCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCEEECC
EGEILLTLADDILPKMARAEYDIASLKEDLHGRLHMAIECHSCFQWLMPAIKEYQVGWPE
CCCEEEEEHHHHHHHHHHHCCHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHCCCCCE
VELDFSSGFGFEPLPALVNGDLDLVITSDIQPRNEVHYEPLFDFEMRLVMATNHPLASKE
EEEECCCCCCCCCCHHHHCCCEEEEEECCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCC
YIEPEDLIDQTMMTYPVQKQRLDVVKHFLSPNNVEPAKWKQAENTLMLIQMVSAGLGVAA
CCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCHHHHHHCCHHHHHHHHHCCCCCHH
LPNWAISEFSRQGLIESKPLGKGLWRRLFAAVRDSEHDKKYLQAFFSTAKQQSQSHLDGI
CCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCC
KMT
CCC
>Mature Secondary Structure
MIELKHLKTLVTLRDTGSLTATANALHLTQSALSHQLKDFETRLGGTVFLRKTRPVKFTS
CCCHHHHHHHEEEECCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCEEECC
EGEILLTLADDILPKMARAEYDIASLKEDLHGRLHMAIECHSCFQWLMPAIKEYQVGWPE
CCCEEEEEHHHHHHHHHHHCCHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHCCCCCE
VELDFSSGFGFEPLPALVNGDLDLVITSDIQPRNEVHYEPLFDFEMRLVMATNHPLASKE
EEEECCCCCCCCCCHHHHCCCEEEEEECCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCC
YIEPEDLIDQTMMTYPVQKQRLDVVKHFLSPNNVEPAKWKQAENTLMLIQMVSAGLGVAA
CCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCHHHHHHCCHHHHHHHHHCCCCCHH
LPNWAISEFSRQGLIESKPLGKGLWRRLFAAVRDSEHDKKYLQAFFSTAKQQSQSHLDGI
CCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCC
KMT
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]