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The map label for this gene is sucB [H]

Identifier: 209694426

GI number: 209694426

Start: 943461

End: 944672

Strand: Direct

Name: sucB [H]

Synonym: VSAL_I0847

Alternate gene names: 209694426

Gene position: 943461-944672 (Clockwise)

Preceding gene: 209694425

Following gene: 209694427

Centisome position: 28.37

GC content: 41.75

Gene sequence:

>1212_bases
ATGACAATCGAAATTCTGGTTCCAGATTTACCTGAATCTGTAGCAGACGCTACTGTAGCTACATGGCATAAAAAACCTGG
TGATACAGTTGAGCGTGATGAAATACTTGTAGATATTGAAACAGATAAAGTGGTTCTTGAAGTACCGGCTCCTGAAGCTG
GTGTACTCGAAGCTATTTTTGAAGATGAAGGTGCGACGGTTCTTTCTAAACAGTTGCTTGCTAAAATCAAACTAGGTGCA
GTTGTTGGTGAGCCAACAAAAGATGTGACGAATGAGACGGAGTCTTCTCCTGATAAACGTCACACCGCATCGTTAGCAGA
AGAGAAAAATGACGCATTAAGCCCTGCGGTACGTCGTTTACTTGGTGAGCATGACATTAAAGCATCAGATGTAAAAGGCA
CTGGTGTTGGTGGTCGAATTACTCGTGAAGATGTTGATGCACATGTTGCAGCATTAAAAGCAACATCAGCAAAAGCCACG
GTATCTAATGAGCCAGCTGCGCCATTAGCTCATCGTAGCCAAAAGCGCGTACCAATGACTCGTCTTCGTAAAACAGTAGC
AAGACGTTTATTGGAAGCGAAAAACAGCACGGCAATGCTAACGACGTTTAATGAAGTAAACATGAAACCAATCATGGAAC
TTCGTAAGCAGTATCAAGAGCAGTTTGAAAAGCGTCACGGCACTCGTTTAGGTTTCATGTCTTTCTATGTGAAAGCGGTA
ACTGAAGCACTTAAACGCTACCCTGAAGTGAACGCATCCATTGATGGTGATGATATTATTTATCATAACTATTTTGATAT
TAGTATGGCGGTATCTACGCCACGTGGTTTAGTGACTCCAGTACTAAAAGACTGTGATGCACTTGGTTTTGCCGATATTG
AAAAAGGCATTAAAGAACTGGCGATTAAAGGCCGTGATGGAAAACTAGCGGTTGAAGATTTAATCGGTGGTAACTTTACT
ATCACTAATGGTGGTGTGTTTGGTTCGCTTATGTCGACACCAATTATTAACCCACCACAAGCGGCAATTTTAGGTATGCA
TAAAATCCAAGACCGTCCAATGGCTGTCAATGGAAAAGTAGAAATTCTACCAATGATGTACCTTGCACTCTCTTATGATC
ACCGTCTAATCGATGGTCGTGAATCAGTAGGTTTCCTTGTAACGATTAAAGAGTTACTTGAAGATCCTGCTCGCTTACTA
CTAGACGTTTAG

Upstream 100 bases:

>100_bases
CCGGCAGTTGGCTATATGTCAGTGCACGTGAAACAACAAAAAGCGTTAGTTGAAGACGCTCTGACCCTAGATAAGAACTA
GAAAGTATAGGAAAACGGAT

Downstream 100 bases:

>100_bases
TAATAAATATCTAGAAATAAACAAAGGCTATCATGCTCACGGTAGCCTCTTATTCAACTTCATTATTGGATAAAAATAGA
AAGATGCCTTAGAGCGTCTT

Product: dihydrolipoamide succinyltransferase

Products: NA

Alternate protein names: 2-oxoglutarate dehydrogenase complex component E2; OGDC-E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 403; Mature: 402

Protein sequence:

>403_residues
MTIEILVPDLPESVADATVATWHKKPGDTVERDEILVDIETDKVVLEVPAPEAGVLEAIFEDEGATVLSKQLLAKIKLGA
VVGEPTKDVTNETESSPDKRHTASLAEEKNDALSPAVRRLLGEHDIKASDVKGTGVGGRITREDVDAHVAALKATSAKAT
VSNEPAAPLAHRSQKRVPMTRLRKTVARRLLEAKNSTAMLTTFNEVNMKPIMELRKQYQEQFEKRHGTRLGFMSFYVKAV
TEALKRYPEVNASIDGDDIIYHNYFDISMAVSTPRGLVTPVLKDCDALGFADIEKGIKELAIKGRDGKLAVEDLIGGNFT
ITNGGVFGSLMSTPIINPPQAAILGMHKIQDRPMAVNGKVEILPMMYLALSYDHRLIDGRESVGFLVTIKELLEDPARLL
LDV

Sequences:

>Translated_403_residues
MTIEILVPDLPESVADATVATWHKKPGDTVERDEILVDIETDKVVLEVPAPEAGVLEAIFEDEGATVLSKQLLAKIKLGA
VVGEPTKDVTNETESSPDKRHTASLAEEKNDALSPAVRRLLGEHDIKASDVKGTGVGGRITREDVDAHVAALKATSAKAT
VSNEPAAPLAHRSQKRVPMTRLRKTVARRLLEAKNSTAMLTTFNEVNMKPIMELRKQYQEQFEKRHGTRLGFMSFYVKAV
TEALKRYPEVNASIDGDDIIYHNYFDISMAVSTPRGLVTPVLKDCDALGFADIEKGIKELAIKGRDGKLAVEDLIGGNFT
ITNGGVFGSLMSTPIINPPQAAILGMHKIQDRPMAVNGKVEILPMMYLALSYDHRLIDGRESVGFLVTIKELLEDPARLL
LDV
>Mature_402_residues
TIEILVPDLPESVADATVATWHKKPGDTVERDEILVDIETDKVVLEVPAPEAGVLEAIFEDEGATVLSKQLLAKIKLGAV
VGEPTKDVTNETESSPDKRHTASLAEEKNDALSPAVRRLLGEHDIKASDVKGTGVGGRITREDVDAHVAALKATSAKATV
SNEPAAPLAHRSQKRVPMTRLRKTVARRLLEAKNSTAMLTTFNEVNMKPIMELRKQYQEQFEKRHGTRLGFMSFYVKAVT
EALKRYPEVNASIDGDDIIYHNYFDISMAVSTPRGLVTPVLKDCDALGFADIEKGIKELAIKGRDGKLAVEDLIGGNFTI
TNGGVFGSLMSTPIINPPQAAILGMHKIQDRPMAVNGKVEILPMMYLALSYDHRLIDGRESVGFLVTIKELLEDPARLLL
DV

Specific function: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components:2- oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2)

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI19923748, Length=257, Percent_Identity=56.8093385214008, Blast_Score=293, Evalue=2e-79,
Organism=Homo sapiens, GI31711992, Length=432, Percent_Identity=30.0925925925926, Blast_Score=175, Evalue=6e-44,
Organism=Homo sapiens, GI203098753, Length=459, Percent_Identity=28.5403050108932, Blast_Score=168, Evalue=7e-42,
Organism=Homo sapiens, GI203098816, Length=459, Percent_Identity=28.322440087146, Blast_Score=167, Evalue=1e-41,
Organism=Homo sapiens, GI110671329, Length=421, Percent_Identity=27.790973871734, Blast_Score=157, Evalue=1e-38,
Organism=Homo sapiens, GI260898739, Length=161, Percent_Identity=39.1304347826087, Blast_Score=110, Evalue=3e-24,
Organism=Escherichia coli, GI1786946, Length=403, Percent_Identity=76.6749379652606, Blast_Score=624, Evalue=1e-180,
Organism=Escherichia coli, GI1786305, Length=432, Percent_Identity=31.712962962963, Blast_Score=169, Evalue=4e-43,
Organism=Caenorhabditis elegans, GI25146366, Length=399, Percent_Identity=43.609022556391, Blast_Score=322, Evalue=3e-88,
Organism=Caenorhabditis elegans, GI17537937, Length=417, Percent_Identity=26.6187050359712, Blast_Score=167, Evalue=8e-42,
Organism=Caenorhabditis elegans, GI17560088, Length=437, Percent_Identity=31.1212814645309, Blast_Score=167, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI17538894, Length=320, Percent_Identity=31.875, Blast_Score=137, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6320352, Length=407, Percent_Identity=41.7690417690418, Blast_Score=313, Evalue=4e-86,
Organism=Saccharomyces cerevisiae, GI6324258, Length=449, Percent_Identity=28.0623608017817, Blast_Score=151, Evalue=1e-37,
Organism=Drosophila melanogaster, GI24645909, Length=229, Percent_Identity=57.6419213973799, Blast_Score=279, Evalue=2e-75,
Organism=Drosophila melanogaster, GI18859875, Length=438, Percent_Identity=28.9954337899543, Blast_Score=161, Evalue=9e-40,
Organism=Drosophila melanogaster, GI24582497, Length=292, Percent_Identity=31.1643835616438, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI20129315, Length=292, Percent_Identity=30.8219178082192, Blast_Score=134, Evalue=1e-31,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053
- InterPro:   IPR006255 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.61 [H]

Molecular weight: Translated: 44065; Mature: 43934

Theoretical pI: Translated: 5.70; Mature: 5.70

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIEILVPDLPESVADATVATWHKKPGDTVERDEILVDIETDKVVLEVPAPEAGVLEAIF
CEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCEEEEECCCCCCHHHHHHH
EDEGATVLSKQLLAKIKLGAVVGEPTKDVTNETESSPDKRHTASLAEEKNDALSPAVRRL
CCCCCHHHHHHHHHHHHHHHEECCCCHHHCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHH
LGEHDIKASDVKGTGVGGRITREDVDAHVAALKATSAKATVSNEPAAPLAHRSQKRVPMT
HCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCCCCCCHH
RLRKTVARRLLEAKNSTAMLTTFNEVNMKPIMELRKQYQEQFEKRHGTRLGFMSFYVKAV
HHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
TEALKRYPEVNASIDGDDIIYHNYFDISMAVSTPRGLVTPVLKDCDALGFADIEKGIKEL
HHHHHHCCCCCCCCCCCCEEEEEEEEEEEEECCCCCHHHHHHHHCCCCCHHHHHHHHHHH
AIKGRDGKLAVEDLIGGNFTITNGGVFGSLMSTPIINPPQAAILGMHKIQDRPMAVNGKV
EECCCCCCEEHHHHCCCCEEEECCCHHHHHHHCCCCCCCHHHHHHHHHHCCCCEEECCCE
EILPMMYLALSYDHRLIDGRESVGFLVTIKELLEDPARLLLDV
EHHHHHHHHHHCCCCEECCCCCCCCEEEHHHHHCCHHHHHCCC
>Mature Secondary Structure 
TIEILVPDLPESVADATVATWHKKPGDTVERDEILVDIETDKVVLEVPAPEAGVLEAIF
EEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCEEEEECCCCCCHHHHHHH
EDEGATVLSKQLLAKIKLGAVVGEPTKDVTNETESSPDKRHTASLAEEKNDALSPAVRRL
CCCCCHHHHHHHHHHHHHHHEECCCCHHHCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHH
LGEHDIKASDVKGTGVGGRITREDVDAHVAALKATSAKATVSNEPAAPLAHRSQKRVPMT
HCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCCCCCCHH
RLRKTVARRLLEAKNSTAMLTTFNEVNMKPIMELRKQYQEQFEKRHGTRLGFMSFYVKAV
HHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
TEALKRYPEVNASIDGDDIIYHNYFDISMAVSTPRGLVTPVLKDCDALGFADIEKGIKEL
HHHHHHCCCCCCCCCCCCEEEEEEEEEEEEECCCCCHHHHHHHHCCCCCHHHHHHHHHHH
AIKGRDGKLAVEDLIGGNFTITNGGVFGSLMSTPIINPPQAAILGMHKIQDRPMAVNGKV
EECCCCCCEEHHHHCCCCEEEECCCHHHHHHHCCCCCCCHHHHHHHHHHCCCCEEECCCE
EILPMMYLALSYDHRLIDGRESVGFLVTIKELLEDPARLLLDV
EHHHHHHHHHHCCCCEECCCCCCCCEEEHHHHHCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]