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The map label for this gene is hemN [H]

Identifier: 209693845

GI number: 209693845

Start: 279179

End: 280552

Strand: Direct

Name: hemN [H]

Synonym: VSAL_I0233

Alternate gene names: 209693845

Gene position: 279179-280552 (Clockwise)

Preceding gene: 209693844

Following gene: 209693846

Centisome position: 8.4

GC content: 40.9

Gene sequence:

>1374_bases
ATGTCAAATCAACAAATAGTATGGGACCAAGCGATCATAGAGAAGTATAACTACTCTGGTCCTCGCTACACCTCTTACCC
AACCGCTTTAGAGTTTCATGAAGCGTATACTCCTGCTGAATTCGACATGGCGTGTACTCAGTATCCTGAGCGACCACTGT
CTCTGTATATCCATATCCCATTTTGTCACAAGCTGTGTTATTTCTGTGGCTGTAATAAAGTCATTACTCGTCATCATCAT
AAAGCGGACATCTACTTAGACTCATTAGAGTTAGAGATCCGTCAACGAGCGGCGTTATTTACCGATCGTAAAGTAACCCA
GTTGCATTTTGGTGGCGGCACACCAACGTTTTTAACGAAAATCCAATTTACTCGCTTAATGAATTTATTACGTGGTGAAT
TTCATTTTGAAGCGGGTGCTGAGATCAGTATCGAAGTTGATCCACGTGAAATTGAATTGGATATGTTGGATCACTTACGT
GATGAAGGGTTCAATCGTTTAAGTATTGGTGTTCAAGACTTTAATAAAGAAGTTCAGTTATTGGTTAACCGTGATCAAGA
TGAAGACTTTATTATCTCTATGGTGCAGCGAGCGAAAGAATTAGGTTTCCGTTCAACTAACCTTGATTTGATTTATGGTT
TACCAAAACAGTCCGCAGAATCATTAGCTATCACGATTAAGCGCGTACTTGAAATGGAACCGGGTCGTTTGTCTGTTTTT
AACTACGCACACATGCCAACCTTATTTGCTGCGCAGCGTAAAATTAAAGACGCCGATTTACCTGCACCCGCGGAAAAATT
GGTGATGCTGCAAGACACCATTGAAACGTTAACTGGTGCCGGTTACCAGTTTATCGGAATGGATCACTTTGCACTGCCTG
ATGATGAGTTAGCGGTCGCGCAACGTGATGGCATTCTTCACCGTAATTTCCAAGGTTACACCACTCAAGGTGAGTGTGAT
TTATTGGGTATGGGTGTATCAGCTATCTCTATGATTGGCGACAGTTACGCACAGAATAAGAAAGATTTAAAAGAATATTA
CGCACAGGTGGATGATAAGCGTCATGCATTGTGGCGTGGTGTTGCACTTGATCAGGATGATTTAATTCGTCGTGAAGTGA
TTAAACAGCTGATCTGTAATTTCACGCTAGATACTAAAGCGATTGAAGTCGAGTTTAATTTAGATTTTGCTACGTACTTT
GCAGAAGATTTTAAATTGCTGCAAACGTTTGTCGATGATGAGCTTGTGTCTCTGCATGAAGGCCGTATTGATGTTGAATT
ACGTGGTCGTTTATTGATCCGTAATATCTGTATGTGTTTTGATAAATACCTTCGTGACCGTGCGCGTCAACAGCAGTTTT
CTAGAGTTATCTAA

Upstream 100 bases:

>100_bases
TTTGATTGTTGCCTTGAAGTTATCGGCTTTAGAAGTGAGATGCTTTATTATGCAAGCATCAAGAAGCGTCTATTTAGATA
TCAAGAGATGGAAGTAAGTC

Downstream 100 bases:

>100_bases
GAGCGGATAAAGCGGGGAAGAGCGCTCCTATGGTCCTGTGAAAACCTGATTGTTTGCCTAAGAATTTGAAGCCGTAGGTG
TTATGCACCTGCGGTTTTTT

Product: coproporphyrinogen III oxidase

Products: NA

Alternate protein names: Coprogen oxidase; Coproporphyrinogenase [H]

Number of amino acids: Translated: 457; Mature: 456

Protein sequence:

>457_residues
MSNQQIVWDQAIIEKYNYSGPRYTSYPTALEFHEAYTPAEFDMACTQYPERPLSLYIHIPFCHKLCYFCGCNKVITRHHH
KADIYLDSLELEIRQRAALFTDRKVTQLHFGGGTPTFLTKIQFTRLMNLLRGEFHFEAGAEISIEVDPREIELDMLDHLR
DEGFNRLSIGVQDFNKEVQLLVNRDQDEDFIISMVQRAKELGFRSTNLDLIYGLPKQSAESLAITIKRVLEMEPGRLSVF
NYAHMPTLFAAQRKIKDADLPAPAEKLVMLQDTIETLTGAGYQFIGMDHFALPDDELAVAQRDGILHRNFQGYTTQGECD
LLGMGVSAISMIGDSYAQNKKDLKEYYAQVDDKRHALWRGVALDQDDLIRREVIKQLICNFTLDTKAIEVEFNLDFATYF
AEDFKLLQTFVDDELVSLHEGRIDVELRGRLLIRNICMCFDKYLRDRARQQQFSRVI

Sequences:

>Translated_457_residues
MSNQQIVWDQAIIEKYNYSGPRYTSYPTALEFHEAYTPAEFDMACTQYPERPLSLYIHIPFCHKLCYFCGCNKVITRHHH
KADIYLDSLELEIRQRAALFTDRKVTQLHFGGGTPTFLTKIQFTRLMNLLRGEFHFEAGAEISIEVDPREIELDMLDHLR
DEGFNRLSIGVQDFNKEVQLLVNRDQDEDFIISMVQRAKELGFRSTNLDLIYGLPKQSAESLAITIKRVLEMEPGRLSVF
NYAHMPTLFAAQRKIKDADLPAPAEKLVMLQDTIETLTGAGYQFIGMDHFALPDDELAVAQRDGILHRNFQGYTTQGECD
LLGMGVSAISMIGDSYAQNKKDLKEYYAQVDDKRHALWRGVALDQDDLIRREVIKQLICNFTLDTKAIEVEFNLDFATYF
AEDFKLLQTFVDDELVSLHEGRIDVELRGRLLIRNICMCFDKYLRDRARQQQFSRVI
>Mature_456_residues
SNQQIVWDQAIIEKYNYSGPRYTSYPTALEFHEAYTPAEFDMACTQYPERPLSLYIHIPFCHKLCYFCGCNKVITRHHHK
ADIYLDSLELEIRQRAALFTDRKVTQLHFGGGTPTFLTKIQFTRLMNLLRGEFHFEAGAEISIEVDPREIELDMLDHLRD
EGFNRLSIGVQDFNKEVQLLVNRDQDEDFIISMVQRAKELGFRSTNLDLIYGLPKQSAESLAITIKRVLEMEPGRLSVFN
YAHMPTLFAAQRKIKDADLPAPAEKLVMLQDTIETLTGAGYQFIGMDHFALPDDELAVAQRDGILHRNFQGYTTQGECDL
LGMGVSAISMIGDSYAQNKKDLKEYYAQVDDKRHALWRGVALDQDDLIRREVIKQLICNFTLDTKAIEVEFNLDFATYFA
EDFKLLQTFVDDELVSLHEGRIDVELRGRLLIRNICMCFDKYLRDRARQQQFSRVI

Specific function: Anaerobic transformation of coproporphyrinogen-III into protoporphyrinogen-IX [H]

COG id: COG0635

COG function: function code H; Coproporphyrinogen III oxidase and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the anaerobic coproporphyrinogen-III oxidase family [H]

Homologues:

Organism=Homo sapiens, GI8922911, Length=282, Percent_Identity=26.5957446808511, Blast_Score=88, Evalue=2e-17,
Organism=Escherichia coli, GI87082341, Length=457, Percent_Identity=71.3347921225383, Blast_Score=684, Evalue=0.0,
Organism=Escherichia coli, GI1789325, Length=243, Percent_Identity=32.5102880658436, Blast_Score=125, Evalue=7e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR004558
- InterPro:   IPR010723
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06969 HemN_C; PF04055 Radical_SAM [H]

EC number: =1.3.99.22 [H]

Molecular weight: Translated: 52860; Mature: 52729

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNQQIVWDQAIIEKYNYSGPRYTSYPTALEFHEAYTPAEFDMACTQYPERPLSLYIHIP
CCCCCEEHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCCHHCCHHHHCCCCCEEEEEECH
FCHKLCYFCGCNKVITRHHHKADIYLDSLELEIRQRAALFTDRKVTQLHFGGGTPTFLTK
HHHHHHHHHCCCHHHHCCCCCCEEEEEHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHH
IQFTRLMNLLRGEFHFEAGAEISIEVDPREIELDMLDHLRDEGFNRLSIGVQDFNKEVQL
HHHHHHHHHHHCCCEECCCCEEEEEECCHHHHHHHHHHHHHCCCCEEEECHHHHCCEEEE
LVNRDQDEDFIISMVQRAKELGFRSTNLDLIYGLPKQSAESLAITIKRVLEMEPGRLSVF
EECCCCCHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCEEEE
NYAHMPTLFAAQRKIKDADLPAPAEKLVMLQDTIETLTGAGYQFIGMDHFALPDDELAVA
EHHCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHH
QRDGILHRNFQGYTTQGECDLLGMGVSAISMIGDSYAQNKKDLKEYYAQVDDKRHALWRG
HHCCCEECCCCCCCCCCCCEEEECCHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHC
VALDQDDLIRREVIKQLICNFTLDTKAIEVEFNLDFATYFAEDFKLLQTFVDDELVSLHE
CCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHC
GRIDVELRGRLLIRNICMCFDKYLRDRARQQQFSRVI
CEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SNQQIVWDQAIIEKYNYSGPRYTSYPTALEFHEAYTPAEFDMACTQYPERPLSLYIHIP
CCCCEEHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCCHHCCHHHHCCCCCEEEEEECH
FCHKLCYFCGCNKVITRHHHKADIYLDSLELEIRQRAALFTDRKVTQLHFGGGTPTFLTK
HHHHHHHHHCCCHHHHCCCCCCEEEEEHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHH
IQFTRLMNLLRGEFHFEAGAEISIEVDPREIELDMLDHLRDEGFNRLSIGVQDFNKEVQL
HHHHHHHHHHHCCCEECCCCEEEEEECCHHHHHHHHHHHHHCCCCEEEECHHHHCCEEEE
LVNRDQDEDFIISMVQRAKELGFRSTNLDLIYGLPKQSAESLAITIKRVLEMEPGRLSVF
EECCCCCHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCEEEE
NYAHMPTLFAAQRKIKDADLPAPAEKLVMLQDTIETLTGAGYQFIGMDHFALPDDELAVA
EHHCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHH
QRDGILHRNFQGYTTQGECDLLGMGVSAISMIGDSYAQNKKDLKEYYAQVDDKRHALWRG
HHCCCEECCCCCCCCCCCCEEEECCHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHC
VALDQDDLIRREVIKQLICNFTLDTKAIEVEFNLDFATYFAEDFKLLQTFVDDELVSLHE
CCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHC
GRIDVELRGRLLIRNICMCFDKYLRDRARQQQFSRVI
CEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7768836; 8346018; 9278503 [H]