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The map label for this gene is opuD [H]

Identifier: 209693721

GI number: 209693721

Start: 132347

End: 133927

Strand: Direct

Name: opuD [H]

Synonym: VSAL_I0098

Alternate gene names: 209693721

Gene position: 132347-133927 (Clockwise)

Preceding gene: 209693720

Following gene: 209693724

Centisome position: 3.98

GC content: 43.26

Gene sequence:

>1581_bases
ATGAGTAGAAATAAGGATAAGTACAGTATAGATAACACGGATTATACTGTTGGACAGGATAACGTTCAAAAATGGGGATT
GGATATTCATAATACCGTCTTCGGCATTAGTGCTGGTTTAATTGCACTCTTCTTAATTGCGGTTGCAGTGACTGATCCAG
AAACCGCTAAGACGGTTCTAAATGGTTTAAAATCACAAATTATCAATGCATTTGATGGTTTGTTTATGTGGTCTGCGAAT
ATCTTTGTGGTTTTCTGCTTAGCATTAATTGTGTCTCCATTCGGTAAGATCCGCCTTGGTGGTGATAAAGCGAAACCCGA
TTATTCGATGATCTCTTGGATTGCGATGTTATTTGCTGCGGGCATGGGCATCGGTCTTATGTTCTGGGGGGTTGCTGAAC
CTGTTGCTTACTTTACCGGTTGGTTTGGAACGCCGTTAGGTGTTGAAGCTTATACTCCAGAAGCAAGGCAACTCGCGCTT
GGTGCGACCATGTTCCACTGGGGTATTCACCCGTGGGCTATTTATGGTGTCGTTGCTTTATCACTGGCCTTCTTTTCTTA
CAATAAAGGCTTACCGCTTTCTATGCGTTCAATCTTCTATCCAATTTTAGGGGATCGTACATGGGGGTGGCCTGGTCACA
TTGTTGATATTCTTGCCGTTCTTGCTACCTTATTTGGTCTTGCGACTTCTCTAGGTCTTGGTGCACAACAAGCAGCAAGT
GGCTTCCAGCATGTGTTTGGTATTGAAGGTGGCTTAGGTTTACAAGTTGCGATTATTTTCATTGTTACGTTACTTGCCGT
TGTGTCGGTAATGCGCGGTATTGATGGTGGCGTAAAAATTATCAGTAATATCAATATGATCATTGCGATAGTGTTTGTTG
TTTTTGTTGCTTTAGTTACCTTTGCGGTTTCTATGGGGGCGATCCCAACAACGATCATGGGTTATATTGAAAATATTATT
CCATTGAGTAATCCAAGTGGTCGTGATGATGAAGCGTGGATGCACGGTTGGACAGTGACTTACTGGGCGTGGTGGATTTC
ATGGTCTCCATTTGTTGGTATGTTTATCGCTCGAGTATCTCGTGGTCGTACGGTTCGTGAGTTCTTGATTGCGGTATTAG
TGGTTCCAACTGTCTTTACTGTGCTGTGGATGTCTACTTTTGGTGGCGTTGCGATTGATCAAGTGATGAATAATGTGGGT
ACATTGGGGACTGATGGATTAACCGACGTCTCTCTGGCGATGTTCCAAATGTTTGATGCTCTGCCTATGGGTAATGTTTT
ATCTATGATTGCTGTTGTTCTGGTGTTGGTCTTCTTTATTACCTCATCGGATTCAGGCTCATTGGTTATCGATAGTATTA
CCGCTGGTGGTAAAGTTGATGCGCCAATTCCACAACGTGTTTTTTGGGCGTTCATGGAAGGGGCAATTGCCGTTGCTCTA
TTATGGGTAGGTGGAACACAAGCGGTTGAAGCGCTTCAAGCAGGTGCGATTTCTACCGCACTGCCGTTTACCATTATTCT
ATTAGTGATGTGTGTCAGTTTGCTTATGGGCATGAGAACAGAGAAACATAATCGTTCTTAA

Upstream 100 bases:

>100_bases
AGTTAGCTTAGTTGTTAGGATCATCCTGTGTGTTTAAATATTTTAAAGCGAGTATTTGAATTAAACCAAAACAGAGAGCT
AAGGCTCGAAGTGAGGAATA

Downstream 100 bases:

>100_bases
TGTTTGAGTAACTGATAAATAAAAAGCCGATGAAGATTATTATCTTCATCGGCTTTTTTATTTCTTGCGGTTTCTATTGC
TTAATTATTGCCAGATTTTT

Product: transporter BCCT family

Products: Proton [Cytoplasm]; choline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 526; Mature: 525

Protein sequence:

>526_residues
MSRNKDKYSIDNTDYTVGQDNVQKWGLDIHNTVFGISAGLIALFLIAVAVTDPETAKTVLNGLKSQIINAFDGLFMWSAN
IFVVFCLALIVSPFGKIRLGGDKAKPDYSMISWIAMLFAAGMGIGLMFWGVAEPVAYFTGWFGTPLGVEAYTPEARQLAL
GATMFHWGIHPWAIYGVVALSLAFFSYNKGLPLSMRSIFYPILGDRTWGWPGHIVDILAVLATLFGLATSLGLGAQQAAS
GFQHVFGIEGGLGLQVAIIFIVTLLAVVSVMRGIDGGVKIISNINMIIAIVFVVFVALVTFAVSMGAIPTTIMGYIENII
PLSNPSGRDDEAWMHGWTVTYWAWWISWSPFVGMFIARVSRGRTVREFLIAVLVVPTVFTVLWMSTFGGVAIDQVMNNVG
TLGTDGLTDVSLAMFQMFDALPMGNVLSMIAVVLVLVFFITSSDSGSLVIDSITAGGKVDAPIPQRVFWAFMEGAIAVAL
LWVGGTQAVEALQAGAISTALPFTIILLVMCVSLLMGMRTEKHNRS

Sequences:

>Translated_526_residues
MSRNKDKYSIDNTDYTVGQDNVQKWGLDIHNTVFGISAGLIALFLIAVAVTDPETAKTVLNGLKSQIINAFDGLFMWSAN
IFVVFCLALIVSPFGKIRLGGDKAKPDYSMISWIAMLFAAGMGIGLMFWGVAEPVAYFTGWFGTPLGVEAYTPEARQLAL
GATMFHWGIHPWAIYGVVALSLAFFSYNKGLPLSMRSIFYPILGDRTWGWPGHIVDILAVLATLFGLATSLGLGAQQAAS
GFQHVFGIEGGLGLQVAIIFIVTLLAVVSVMRGIDGGVKIISNINMIIAIVFVVFVALVTFAVSMGAIPTTIMGYIENII
PLSNPSGRDDEAWMHGWTVTYWAWWISWSPFVGMFIARVSRGRTVREFLIAVLVVPTVFTVLWMSTFGGVAIDQVMNNVG
TLGTDGLTDVSLAMFQMFDALPMGNVLSMIAVVLVLVFFITSSDSGSLVIDSITAGGKVDAPIPQRVFWAFMEGAIAVAL
LWVGGTQAVEALQAGAISTALPFTIILLVMCVSLLMGMRTEKHNRS
>Mature_525_residues
SRNKDKYSIDNTDYTVGQDNVQKWGLDIHNTVFGISAGLIALFLIAVAVTDPETAKTVLNGLKSQIINAFDGLFMWSANI
FVVFCLALIVSPFGKIRLGGDKAKPDYSMISWIAMLFAAGMGIGLMFWGVAEPVAYFTGWFGTPLGVEAYTPEARQLALG
ATMFHWGIHPWAIYGVVALSLAFFSYNKGLPLSMRSIFYPILGDRTWGWPGHIVDILAVLATLFGLATSLGLGAQQAASG
FQHVFGIEGGLGLQVAIIFIVTLLAVVSVMRGIDGGVKIISNINMIIAIVFVVFVALVTFAVSMGAIPTTIMGYIENIIP
LSNPSGRDDEAWMHGWTVTYWAWWISWSPFVGMFIARVSRGRTVREFLIAVLVVPTVFTVLWMSTFGGVAIDQVMNNVGT
LGTDGLTDVSLAMFQMFDALPMGNVLSMIAVVLVLVFFITSSDSGSLVIDSITAGGKVDAPIPQRVFWAFMEGAIAVALL
WVGGTQAVEALQAGAISTALPFTIILLVMCVSLLMGMRTEKHNRS

Specific function: High-affinity uptake of glycine betaine [H]

COG id: COG1292

COG function: function code M; Choline-glycine betaine transporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the BCCT transporter (TC 2.A.15) family [H]

Homologues:

Organism=Escherichia coli, GI1786506, Length=510, Percent_Identity=34.7058823529412, Blast_Score=315, Evalue=5e-87,
Organism=Escherichia coli, GI1786224, Length=492, Percent_Identity=27.2357723577236, Blast_Score=170, Evalue=2e-43,
Organism=Escherichia coli, GI1788102, Length=438, Percent_Identity=28.9954337899543, Blast_Score=162, Evalue=4e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000060
- InterPro:   IPR018093 [H]

Pfam domain/function: PF02028 BCCT [H]

EC number: NA

Molecular weight: Translated: 56761; Mature: 56629

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: PS01303 BCCT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
SRNKDKYSIDNTDYTVGQDNVQKWGLDIHNTVFGISAGLIALFLIAVAVTDPETAKTVL
CCCCCCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH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HHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; choline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + choline [Periplasm] = Proton [Cytoplasm] + choline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8752321; 9387221; 9384377 [H]